BMC Developmental Biology
... Moreover, Eya genes are associated with the proper development of muscles. Drosophila eya mutants show musculature defects [16]; mouse embryos deficient for Eya1 and Six1 have a complete absence of all hypaxial muscle and a severe reduction of epaxial muscle [17]. While neither in Eya1-/- nor in Eya ...
... Moreover, Eya genes are associated with the proper development of muscles. Drosophila eya mutants show musculature defects [16]; mouse embryos deficient for Eya1 and Six1 have a complete absence of all hypaxial muscle and a severe reduction of epaxial muscle [17]. While neither in Eya1-/- nor in Eya ...
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... alleles of the PEGs. However, a deletion on the paternal chromosome does not have this effect (Lin et al., 2003). The mechanism by which the IG-DMR regulates Dlk1-Gtl2 imprinting is unclear. It is likely that the IG-DMR acts as a positive regulator activating Gtl2 transcription and its downstream no ...
... alleles of the PEGs. However, a deletion on the paternal chromosome does not have this effect (Lin et al., 2003). The mechanism by which the IG-DMR regulates Dlk1-Gtl2 imprinting is unclear. It is likely that the IG-DMR acts as a positive regulator activating Gtl2 transcription and its downstream no ...
Synthetic Chemical Inducers and Genetic
... can be metabolized further by an assimilatory aerobic pathway, to pyruvate via lactate, or an anaerobic pathway, to propane-1− 2-diol.42,43 This efficient metabolism of L-rhamnose gives rise to the one major limitation of rhaBAD as an expression system: a decrease in inducer concentration over time, w ...
... can be metabolized further by an assimilatory aerobic pathway, to pyruvate via lactate, or an anaerobic pathway, to propane-1− 2-diol.42,43 This efficient metabolism of L-rhamnose gives rise to the one major limitation of rhaBAD as an expression system: a decrease in inducer concentration over time, w ...
Control of the acetamidase gene of Mycobacterium smegmatis by
... The mode of regulation of the M. smegmatis acetamidase is complex, involving three regulatory proteins and four promoters. The construction of several deletion strains missing di¡erent combinations of the acetamidase regulatory genes has enabled us to con¢rm that AmiA, C and D are all involved in th ...
... The mode of regulation of the M. smegmatis acetamidase is complex, involving three regulatory proteins and four promoters. The construction of several deletion strains missing di¡erent combinations of the acetamidase regulatory genes has enabled us to con¢rm that AmiA, C and D are all involved in th ...
Seed-Specific Gene Activation Mediated by the Cre//ox Site
... activation of a 8-glucuronidase marker gene, by removal of a loxbounded blocking fragment, allowed the visualization of the activation process. By using seed-specific promoters, the timing and efficiency of gene activation could be followed within the developing tobacco (Nicotiana tabacum) embryo. T ...
... activation of a 8-glucuronidase marker gene, by removal of a loxbounded blocking fragment, allowed the visualization of the activation process. By using seed-specific promoters, the timing and efficiency of gene activation could be followed within the developing tobacco (Nicotiana tabacum) embryo. T ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... promoter regions is generally thought to repress gene expression, while gene body methylation is generally associated with active gene expression and has been suggested to regulate splicing 6-8. In most cell types, the majority of CpGs in the genome (on average 70-80%) is typically methylated 9. Of ...
... promoter regions is generally thought to repress gene expression, while gene body methylation is generally associated with active gene expression and has been suggested to regulate splicing 6-8. In most cell types, the majority of CpGs in the genome (on average 70-80%) is typically methylated 9. Of ...
1 Tuning of recombinant protein expression in Escherichia
... polymerases on DNA and of ribosomes on mRNA are known to depend on the transcription and translation initiation rates, which, in turn, are determined by the strengths of the promoter and of the ribosome binding site (RBS). For example, it was shown that the use of a strong RBS with a high initiation ...
... polymerases on DNA and of ribosomes on mRNA are known to depend on the transcription and translation initiation rates, which, in turn, are determined by the strengths of the promoter and of the ribosome binding site (RBS). For example, it was shown that the use of a strong RBS with a high initiation ...
Glioma heterogeneity and the LAT-1
... SLC3A2 Increased expression in GBM compared with normal brain and Low grade glioma Up-regulation is associated with a significantly worse prognosis when considering all glioma Particular prognostic significance in oligodendroglioma. SLC7A5 Expression not upregulated in GBM increased expres ...
... SLC3A2 Increased expression in GBM compared with normal brain and Low grade glioma Up-regulation is associated with a significantly worse prognosis when considering all glioma Particular prognostic significance in oligodendroglioma. SLC7A5 Expression not upregulated in GBM increased expres ...
Cloning and expression analysis of follicle
... (7TM) helices and a carboxy-terminal intracellular tail (Hsu et al. 2000). The cDNA and sequences of the GTHs receptor have been determined for a number of fish species, such as Oncorhynchus masou rhodurus (Oba et al. 1999b) and later from many other teleost species such as Clarias gariepinus (Boger ...
... (7TM) helices and a carboxy-terminal intracellular tail (Hsu et al. 2000). The cDNA and sequences of the GTHs receptor have been determined for a number of fish species, such as Oncorhynchus masou rhodurus (Oba et al. 1999b) and later from many other teleost species such as Clarias gariepinus (Boger ...
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... the LIM domains on Xlim-1; Ldb1 (also known as NLI and CLIM2) being one such factor (Agulnick et al., 1996; Jurata et al., 1996; Bach et al., 1997). Ldb1 contains a selfdimerization domain and a LIM interaction domain (Jurata and Gill, 1997; Breen et al., 1998), and a dimer of Ldb1 has been shown to ...
... the LIM domains on Xlim-1; Ldb1 (also known as NLI and CLIM2) being one such factor (Agulnick et al., 1996; Jurata et al., 1996; Bach et al., 1997). Ldb1 contains a selfdimerization domain and a LIM interaction domain (Jurata and Gill, 1997; Breen et al., 1998), and a dimer of Ldb1 has been shown to ...
A Dnmt2-like protein mediates DNA methylation in
... were spotted onto a membrane and stained for 5methylcytosine and DNA, respectively. This revealed a clearly differential staining pattern for 5-methylcytosine, while the DNA staining pattern was equal for all samples (Fig. 1E). As an additional control for the specificity of our immunostaining proto ...
... were spotted onto a membrane and stained for 5methylcytosine and DNA, respectively. This revealed a clearly differential staining pattern for 5-methylcytosine, while the DNA staining pattern was equal for all samples (Fig. 1E). As an additional control for the specificity of our immunostaining proto ...
Phox2b and Midkine/Alk signaling in the control of sympathetic
... proteins, ectopically expressed in proliferating, embryonic sympathetic neurons. Phox2bwt displays a strong antiproliferative effect, which is lost in all Phox2bmut NB variants analyzed. In contrast, Phox2bmut elicits an increase in sympathetic neuron proliferation, both in vitro and in vivo. The el ...
... proteins, ectopically expressed in proliferating, embryonic sympathetic neurons. Phox2bwt displays a strong antiproliferative effect, which is lost in all Phox2bmut NB variants analyzed. In contrast, Phox2bmut elicits an increase in sympathetic neuron proliferation, both in vitro and in vivo. The el ...
NIH Public Access
... offspring over which both paternal and maternal parents are competing. A decade ago, a specific class of mutants was identified that shows fertilization-independent seed development (for review, see [2]). Later studies revealed that these mutants are impaired in the Polycomb group (PcG) complex in t ...
... offspring over which both paternal and maternal parents are competing. A decade ago, a specific class of mutants was identified that shows fertilization-independent seed development (for review, see [2]). Later studies revealed that these mutants are impaired in the Polycomb group (PcG) complex in t ...
S2 File.
... Fig A: Results of optical density measurement (top) and the crystal violet assay (bottom) for replicates (n = 3) of the wells that were harvested for RNA sequencing. Wells without NaCl addition were harvested after 1 day of incubation, while wells with NaCl addition were harvested after 2 days, to ...
... Fig A: Results of optical density measurement (top) and the crystal violet assay (bottom) for replicates (n = 3) of the wells that were harvested for RNA sequencing. Wells without NaCl addition were harvested after 1 day of incubation, while wells with NaCl addition were harvested after 2 days, to ...
ELMER: An R/Bioconductor Tool Inferring Regulatory Element
... to identify enhancers, and correlates enhancer state with expression of nearby genes to identify one or more transcriptional targets. Transcription factor (TF) binding site analysis of enhancers is coupled with expression analysis of all TFs to infer upstream regulators. This package can be easily a ...
... to identify enhancers, and correlates enhancer state with expression of nearby genes to identify one or more transcriptional targets. Transcription factor (TF) binding site analysis of enhancers is coupled with expression analysis of all TFs to infer upstream regulators. This package can be easily a ...
Rapid Selection of Multiple Gene Integrant for the Production of
... acid and DNA sequence of them are different and this was the only reason of expression level difference. The previous reports about the expression of hirudin variants were studied about only one variant at one time even though two of them were tested in one organism. Several cases about the correlat ...
... acid and DNA sequence of them are different and this was the only reason of expression level difference. The previous reports about the expression of hirudin variants were studied about only one variant at one time even though two of them were tested in one organism. Several cases about the correlat ...
The role of regulatory variation in complex traits and
... homologous chromosome. Therefore, cis-eQTLs can be detected in heterozygous individuals by quantifying the relative expression levels of the two alleles, for example, by counting the number of times each allele is observed in RNA sequencing data. If there is an imbalance in the expression levels of ...
... homologous chromosome. Therefore, cis-eQTLs can be detected in heterozygous individuals by quantifying the relative expression levels of the two alleles, for example, by counting the number of times each allele is observed in RNA sequencing data. If there is an imbalance in the expression levels of ...
as a PDF
... the MLE protein (encoded by maleless), as well as the acetylation pattern of histone H4, in male germline cells. Although MLE is known to be involved in X chromosome hypertranscription in somatic cells, presumably through the recruitment of histone acetylation factors [31,32], it does not associate ...
... the MLE protein (encoded by maleless), as well as the acetylation pattern of histone H4, in male germline cells. Although MLE is known to be involved in X chromosome hypertranscription in somatic cells, presumably through the recruitment of histone acetylation factors [31,32], it does not associate ...
Notch activity induces Nodal expression and mediates the
... 3H). Taken together, our results show that there are two conserved RBPjk recognition sites in the Nodal promoter, and that both sites are required for perinodal expression of Nodal in the mouse embryo. One of the earliest determinants of LR establishment in the chick embryo is the left-sided express ...
... 3H). Taken together, our results show that there are two conserved RBPjk recognition sites in the Nodal promoter, and that both sites are required for perinodal expression of Nodal in the mouse embryo. One of the earliest determinants of LR establishment in the chick embryo is the left-sided express ...
Aberrant DNA methylation in cancer: potential clinical
... cell division, because it exhibits much higher activity on hemimethylated DNA than on unmethylated DNA (Ref. 11). Subsequently two more enzymes – DNMT3a and 3b – were also cloned (Ref. 12). Unlike DNMT1, these show no preference for hemimethylated DNA, and, based on inactivation of the DNMT3a and 3b ...
... cell division, because it exhibits much higher activity on hemimethylated DNA than on unmethylated DNA (Ref. 11). Subsequently two more enzymes – DNMT3a and 3b – were also cloned (Ref. 12). Unlike DNMT1, these show no preference for hemimethylated DNA, and, based on inactivation of the DNMT3a and 3b ...