Cloning and Characterization of Novel CIS Family
... phosphorylation of the cytoplasmic domains and subsequent recruitment of various signaling proteins to the receptor complex [2]. Among these proteins are members of the STAT family of transcription factors [3]. The tyrosine-phosphorylated STATs form homoor hetero-dimers and translocate into the nucl ...
... phosphorylation of the cytoplasmic domains and subsequent recruitment of various signaling proteins to the receptor complex [2]. Among these proteins are members of the STAT family of transcription factors [3]. The tyrosine-phosphorylated STATs form homoor hetero-dimers and translocate into the nucl ...
Rb is required for progression through myogenic differentiation but
... (MHC) is reduced. In addition, serum restimulation of these differentiated pRb-deficient myoblasts results in BrdU incorporation and, thus, S-phase entry and DNA synthesis. However, these cells are unable to enter mitosis. Moreover, forced expression of MyoD in a variety of Rb/ fibroblastic cells ...
... (MHC) is reduced. In addition, serum restimulation of these differentiated pRb-deficient myoblasts results in BrdU incorporation and, thus, S-phase entry and DNA synthesis. However, these cells are unable to enter mitosis. Moreover, forced expression of MyoD in a variety of Rb/ fibroblastic cells ...
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... coding S. pombe’s single Argonaute, Dicer, and RNAdependent RNA polymerase homologs, leads to loss of silencing of heterochromatic repeats at the centromere. The Drosophila piwi mutation also affects transcriptional gene silencing (Pal-Bhadra et al. 2002). In addition, a Tetrahymena piwi-related gen ...
... coding S. pombe’s single Argonaute, Dicer, and RNAdependent RNA polymerase homologs, leads to loss of silencing of heterochromatic repeats at the centromere. The Drosophila piwi mutation also affects transcriptional gene silencing (Pal-Bhadra et al. 2002). In addition, a Tetrahymena piwi-related gen ...
Describe the process of cell division in prokaryotic cells.
... chromosomes are photographed when they are highly condensed, then photos of the individual chromosomes are cut out and arranged in order of decreasing size: ...
... chromosomes are photographed when they are highly condensed, then photos of the individual chromosomes are cut out and arranged in order of decreasing size: ...
Unusual mutations in high functioning fragile X males
... available signals of repeats between 45 and 300, this sample is probably representative of premutations and full mutations in the given size interval. Expansion size was measured as CGG repeat index27 given by the difference in size (base pairs) of normal and mutant bands, dividing by 3, and adding ...
... available signals of repeats between 45 and 300, this sample is probably representative of premutations and full mutations in the given size interval. Expansion size was measured as CGG repeat index27 given by the difference in size (base pairs) of normal and mutant bands, dividing by 3, and adding ...
Apoptosis Gene Expression Profiling of Ex Vivo
... paraffin. Low labeling indices were observed under different storage conditions as assessed by immunostaining immunostaining with caspasecaspase-3 (range 0.0%0.0%1.2%) and a DNA fragmentation assay (TUNEL, range 0.0%0.0%-2.3%). Cellular results were confirmed at the gene expression level using the H ...
... paraffin. Low labeling indices were observed under different storage conditions as assessed by immunostaining immunostaining with caspasecaspase-3 (range 0.0%0.0%1.2%) and a DNA fragmentation assay (TUNEL, range 0.0%0.0%-2.3%). Cellular results were confirmed at the gene expression level using the H ...
Genome-scale profiling of histone H3.3 replacement patterns
... modification and of active transcription on tiling arrays. For chromosome arm 2L, data sets are available from chromatin immunoprecipitation experiments assayed on tiling microarrays at 1.5-kb resolution3,15. Both dimethylation at Lys4 of H3 (H3K4me2) and RNA polymerase II (Pol II; using an antibody ...
... modification and of active transcription on tiling arrays. For chromosome arm 2L, data sets are available from chromatin immunoprecipitation experiments assayed on tiling microarrays at 1.5-kb resolution3,15. Both dimethylation at Lys4 of H3 (H3K4me2) and RNA polymerase II (Pol II; using an antibody ...
Insertion of liver enriched transcription
... (EBV)-based vector, p706. The results of transfection revealed that unexpectedly the HNF-4 binding sites in these constructs act as a repressor rather than enhancer of the liver-specific expression of the luciferase gene. Key words: Liver, transcription-factor-HNF-4, simian virus (Sv40), vector ...
... (EBV)-based vector, p706. The results of transfection revealed that unexpectedly the HNF-4 binding sites in these constructs act as a repressor rather than enhancer of the liver-specific expression of the luciferase gene. Key words: Liver, transcription-factor-HNF-4, simian virus (Sv40), vector ...
Dual roles of lineage restricted transcription factors
... was also described that MITF recruits the SWI/SNF complex to the promoters of differentiation-related targets Tyrosinase and TRP1, but not to cell maintenance genes TBX2 and BCL2.27,28 This mechanism is suggested to drive selective expression of MITF target genes. SWI/SNF complexes are ATP-dependent ...
... was also described that MITF recruits the SWI/SNF complex to the promoters of differentiation-related targets Tyrosinase and TRP1, but not to cell maintenance genes TBX2 and BCL2.27,28 This mechanism is suggested to drive selective expression of MITF target genes. SWI/SNF complexes are ATP-dependent ...
Treatment Planning Considerations and Modifications
... and can lasts from hours to days (Matsui, 2008). There are several ways to minimize or even eliminate sickle crises, which include keeping the body hydrated, avoiding sources of infection, and preventing a loss of oxygen from the tissues. Emotional stress, high altitudes, and challenging physical ac ...
... and can lasts from hours to days (Matsui, 2008). There are several ways to minimize or even eliminate sickle crises, which include keeping the body hydrated, avoiding sources of infection, and preventing a loss of oxygen from the tissues. Emotional stress, high altitudes, and challenging physical ac ...
Transcription Factor Positive Regulatory Domain 4 (PRDM4) recruits Protein Arginine
... neurogenesis can be recapitulated by individua l mouse cortical NSCs isolated at embryonic day 10 (E10) and cultured at low density in vitro (2,3). This suggests that the temporal program of NSC division and cell fate specification is cell-intrinsic, but the molecular nature of the program is not kn ...
... neurogenesis can be recapitulated by individua l mouse cortical NSCs isolated at embryonic day 10 (E10) and cultured at low density in vitro (2,3). This suggests that the temporal program of NSC division and cell fate specification is cell-intrinsic, but the molecular nature of the program is not kn ...
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... There is reason to believe that the connection between Argonaute family members and stem cell character might also exist in mammals. Human Hiwi is expressed in primitive hematopoietic progenitors (CD34+ bone marrow stem cells) but not in more differentiated hematopoietic lineages. In fact, loss of H ...
... There is reason to believe that the connection between Argonaute family members and stem cell character might also exist in mammals. Human Hiwi is expressed in primitive hematopoietic progenitors (CD34+ bone marrow stem cells) but not in more differentiated hematopoietic lineages. In fact, loss of H ...
Leukaemia Section Chronic myelogenous leukaemia (CML) Atlas of Genetics and Cytogenetics
... lead to restoration of PP2A levels and inhibition of several signalling pathways controlled by BCR-ABL, including AKT, Jak2, STAT and Myc. The global result in different models leads to the reversion of BC phenotype (reduced proliferation, increased differentiation) even in imatinib resistant (inclu ...
... lead to restoration of PP2A levels and inhibition of several signalling pathways controlled by BCR-ABL, including AKT, Jak2, STAT and Myc. The global result in different models leads to the reversion of BC phenotype (reduced proliferation, increased differentiation) even in imatinib resistant (inclu ...
GIGAS CELL1, a Novel Negative Regulator of the
... the mutant genome. We found base substitutions in At3g57860 in both gig1-1 and gig1-2 (Figure 5A). The gig1-1 mutation changes Val at position 42 into Met, while, in gig1-2, the G-to-A substitution at the 39 splice site of intron 2 results in splicing variants encoding truncated GIG1 (see Supplement ...
... the mutant genome. We found base substitutions in At3g57860 in both gig1-1 and gig1-2 (Figure 5A). The gig1-1 mutation changes Val at position 42 into Met, while, in gig1-2, the G-to-A substitution at the 39 splice site of intron 2 results in splicing variants encoding truncated GIG1 (see Supplement ...
MCB317 Topic 10, part 4, A Story of Txn Sp14
... histones bind and on which bases and strands of the DNA faces outward on the nucleosome surface and which face inward ...
... histones bind and on which bases and strands of the DNA faces outward on the nucleosome surface and which face inward ...
Gene expression regulation and the lactase gene
... Fig. 22. Structure and biosynthesis of the lactase protein. Biosynthesis of the lactase protein. In differentiated small intestinal enterocytes the 49.3 kb lactase gene is transcribed into a 6.2 kb mRNA (step a). Translation of lactase mRNA results in the pre-pro-LPH which is translocated over the e ...
... Fig. 22. Structure and biosynthesis of the lactase protein. Biosynthesis of the lactase protein. In differentiated small intestinal enterocytes the 49.3 kb lactase gene is transcribed into a 6.2 kb mRNA (step a). Translation of lactase mRNA results in the pre-pro-LPH which is translocated over the e ...
Rat embryonic ectoderm as renal isograft - Development
... the Miillerian ducts in the male mouse embryos, may involve this mechanism (Trelstad, Hayashi, Hayashi & Donahoe, 1982; Paranko, Pelliniemi & Foidart, 1984). On the other hand, the formation of mesenchyme through an analogoue of the primitive streak is always attained when the early mouse embryo (or ...
... the Miillerian ducts in the male mouse embryos, may involve this mechanism (Trelstad, Hayashi, Hayashi & Donahoe, 1982; Paranko, Pelliniemi & Foidart, 1984). On the other hand, the formation of mesenchyme through an analogoue of the primitive streak is always attained when the early mouse embryo (or ...
quality - National Center for Biomedical Ontology
... Evidence of discoverability - enables positive outputs that were unanticipated Continual, iterative process that occurs at each stage of development and growth, fully integrated into the lifecycle Evidence of community acceptance, that more data is being provided continuously Contains suffic ...
... Evidence of discoverability - enables positive outputs that were unanticipated Continual, iterative process that occurs at each stage of development and growth, fully integrated into the lifecycle Evidence of community acceptance, that more data is being provided continuously Contains suffic ...
TARGETING YOUR DNA WITH THE CRE/LOX SYSTEM
... disadvantages of the Cre/lox system are that not all tissue specific promoters are perfectly specific [4]. Basal levels of expression in other cell types can sometimes cause unintended gene expression. In addition, the establishment of transgenic systems with inserted genes requires a significant am ...
... disadvantages of the Cre/lox system are that not all tissue specific promoters are perfectly specific [4]. Basal levels of expression in other cell types can sometimes cause unintended gene expression. In addition, the establishment of transgenic systems with inserted genes requires a significant am ...
The CpG island methylator phenotype (CIMP) in
... promoter CpG island sites, resulting in the inactivation of several tumor suppressor genes or other tumor-related genes (17). Role of Epigenetics in normal cells and cancer cells Epigenetic regulation of gene expression is a general key mechanism that is operative in normal tissues and has an import ...
... promoter CpG island sites, resulting in the inactivation of several tumor suppressor genes or other tumor-related genes (17). Role of Epigenetics in normal cells and cancer cells Epigenetic regulation of gene expression is a general key mechanism that is operative in normal tissues and has an import ...
Gene Section POU4F1 (POU class 4 homeobox 1) in Oncology and Haematology
... whilst antagonising p53 mediated expression of proapoptotic target genes, Bax and Noxa. Brn-3a other interacting partner includes Rin1 (on target gene, Egr1), HIPK1 (alters TrkA expression), EWS - Fli1 fusion protein (represses Brn-3a mediated effects on survival / differentiation genes). In additio ...
... whilst antagonising p53 mediated expression of proapoptotic target genes, Bax and Noxa. Brn-3a other interacting partner includes Rin1 (on target gene, Egr1), HIPK1 (alters TrkA expression), EWS - Fli1 fusion protein (represses Brn-3a mediated effects on survival / differentiation genes). In additio ...
RNAi in Plants: An Argonaute-Centered View
... 2015). It has been proposed that the chaperone machinery supports a loading-competent state of fly Ago2, allowing it to accept siRNA duplexes from Dcr-2-R2D2 (Miyoshi et al., 2010; Iwasaki et al., 2015). In plants, studies using tobacco BY-2 lysate demonstrated that HSP90 binds to Arabidopsis AGO1 an ...
... 2015). It has been proposed that the chaperone machinery supports a loading-competent state of fly Ago2, allowing it to accept siRNA duplexes from Dcr-2-R2D2 (Miyoshi et al., 2010; Iwasaki et al., 2015). In plants, studies using tobacco BY-2 lysate demonstrated that HSP90 binds to Arabidopsis AGO1 an ...
In vitro fertilization – a tool to dissect cell specification from a
... of their expression. It is generally accepted that a wide range of developmental events are epigenetically controlled. A characteristic feature of such underlying processes is that it is not associated with changes in DNA sequences but is under the control of heritable changes in gene expression. Im ...
... of their expression. It is generally accepted that a wide range of developmental events are epigenetically controlled. A characteristic feature of such underlying processes is that it is not associated with changes in DNA sequences but is under the control of heritable changes in gene expression. Im ...
Supplementary Information (doc 7548K)
... that was heterozygous by exome sequencing (minimum coverage of 10 reads with >3 reads for each allele) showed 80% usage of a single allele. Patient samples where GATA2 gene expression was too low to assess allelic usage or where only homozygous SNPs were present were reported as non-informative (NI ...
... that was heterozygous by exome sequencing (minimum coverage of 10 reads with >3 reads for each allele) showed 80% usage of a single allele. Patient samples where GATA2 gene expression was too low to assess allelic usage or where only homozygous SNPs were present were reported as non-informative (NI ...