Distribution of Chromosome Constitutive
... the current study shows that one-half of each X chromo some is totally heterochromatic. With thymidine-3H and autoradiographic technique, only one of the X chromo somes is late labeling and becomes completely inactive in a random fashion as suggested by Lyon (15). In 2 of the tumors studied, BP45t a ...
... the current study shows that one-half of each X chromo some is totally heterochromatic. With thymidine-3H and autoradiographic technique, only one of the X chromo somes is late labeling and becomes completely inactive in a random fashion as suggested by Lyon (15). In 2 of the tumors studied, BP45t a ...
Biochemical and genetic characterization of the
... complex. In agreement with our previous study (21), the M1-2B extract contained two species of DNA ligase that could be resolved by hydroxyapatite chromatography (Fig. 2B, lanes 1 and 2). Cdc9 DNA ligase, which is eluted from the hydroxyapatite column by 200 mM KPO4, was present at similar levels in ...
... complex. In agreement with our previous study (21), the M1-2B extract contained two species of DNA ligase that could be resolved by hydroxyapatite chromatography (Fig. 2B, lanes 1 and 2). Cdc9 DNA ligase, which is eluted from the hydroxyapatite column by 200 mM KPO4, was present at similar levels in ...
DNA Structure: Gumdrop Modeling Student Version
... This packaging not only helps the DNA to fit into the nucleus, it also helps to control which parts of the DNA are expressed, or turned on. Think about it – a blood cell and a muscle cell look very different, and have very different jobs to do (see the pictures below), but they have the exact same D ...
... This packaging not only helps the DNA to fit into the nucleus, it also helps to control which parts of the DNA are expressed, or turned on. Think about it – a blood cell and a muscle cell look very different, and have very different jobs to do (see the pictures below), but they have the exact same D ...
Unearthing the Roles of Imprinted Genes in the Placenta
... The development of lineage-specific cells begins with the differentiation of the trophoblast lineage and the inner cell mass [16]. This event depends on epigenetic modifications that control the expression of particular genes, allowing cells to develop and differentiate into specific cells and tissues ...
... The development of lineage-specific cells begins with the differentiation of the trophoblast lineage and the inner cell mass [16]. This event depends on epigenetic modifications that control the expression of particular genes, allowing cells to develop and differentiate into specific cells and tissues ...
AML1-ETO expression is directly involved in the development of
... Result conDetection of AML1-ETO expression in the spleen and bone marrow cells from transgenic mice without ENU treatment (Tg), leukemic transgenic mice (Tg -ENU-AML or Tg-ENU-ALL), and wild-type (WT) mice. Representative results are shown. Kasumi-1 cell line RNA was used as a positive control. (A) ...
... Result conDetection of AML1-ETO expression in the spleen and bone marrow cells from transgenic mice without ENU treatment (Tg), leukemic transgenic mice (Tg -ENU-AML or Tg-ENU-ALL), and wild-type (WT) mice. Representative results are shown. Kasumi-1 cell line RNA was used as a positive control. (A) ...
Methyltransferase G9A regulates T cell differentiation during
... CD4+ T cells (Figure 1G), the total number of Foxp3+ cells was comparable in mice that received G9afl/fl and G9a–/– T cells (Figure 2D). Functionally, we failed to observe any differences in the frequency or suppressive capacity of thymus-derived Tregs between G9afl/fl and G9a–/– mice (Figure 2, E a ...
... CD4+ T cells (Figure 1G), the total number of Foxp3+ cells was comparable in mice that received G9afl/fl and G9a–/– T cells (Figure 2D). Functionally, we failed to observe any differences in the frequency or suppressive capacity of thymus-derived Tregs between G9afl/fl and G9a–/– mice (Figure 2, E a ...
supplemental figure legend and Materials and Methods
... CCND1 expression among small cell carcinoma cases (#22 and 24) compared to most non-small cell CRPC cases (with the exception of #11, 32, 25, as described in A). Supplementary Figure S3: Short-term and neo-adjuvant androgen deprivation therapy (ADT) has not exhibited direct effects on CCND1, CDKN2A, ...
... CCND1 expression among small cell carcinoma cases (#22 and 24) compared to most non-small cell CRPC cases (with the exception of #11, 32, 25, as described in A). Supplementary Figure S3: Short-term and neo-adjuvant androgen deprivation therapy (ADT) has not exhibited direct effects on CCND1, CDKN2A, ...
Widespread Organ Expression of the Rat Proenkephalin Gene
... The recent demonstration of proenkephalin expression by developing astrocytes and neurons (4, 5, 10) suggests that the peptide products derived from this precursor may regulate cell differentiation or proliferation within the brain. Growth-promoting effects of enkephalins and other opioid peptides o ...
... The recent demonstration of proenkephalin expression by developing astrocytes and neurons (4, 5, 10) suggests that the peptide products derived from this precursor may regulate cell differentiation or proliferation within the brain. Growth-promoting effects of enkephalins and other opioid peptides o ...
Yeast whole-genome analysis of conserved regulatory motifs
... The NF-κB motif is enriched in H3K4me2 regions found uniquely in GM12878 cells It is likewise enriched in the uniquely bound regions for other active marks Conversely, it is enriched in the uniquely unbound regions for the repressive mark H3K27me3 We find that NF-κB is also over expressed in GM12878 ...
... The NF-κB motif is enriched in H3K4me2 regions found uniquely in GM12878 cells It is likewise enriched in the uniquely bound regions for other active marks Conversely, it is enriched in the uniquely unbound regions for the repressive mark H3K27me3 We find that NF-κB is also over expressed in GM12878 ...
Mitosis and the Cell Cycle
... of a multicellular organism are formed during growth and development. Cell division is used for repair and replacement of cells and tissues during one's lifetime. Asexual reproduction, a means of making more individuals for many groups of organisms, is also accomplished by cell division. In our disc ...
... of a multicellular organism are formed during growth and development. Cell division is used for repair and replacement of cells and tissues during one's lifetime. Asexual reproduction, a means of making more individuals for many groups of organisms, is also accomplished by cell division. In our disc ...
Flexible friends
... To do this, the researchers removed the inner cell mass and grew the cells in a flat dish create a cell line, a set of genetically identical cells. This required a nutrient-rich liquid and some mouse cells to serve as feeder or support cells. The isolated cells grew and grew. Indeed, part of the def ...
... To do this, the researchers removed the inner cell mass and grew the cells in a flat dish create a cell line, a set of genetically identical cells. This required a nutrient-rich liquid and some mouse cells to serve as feeder or support cells. The isolated cells grew and grew. Indeed, part of the def ...
Genetic and epigenetic processes in seed development Allan R
... functions in the developing endosperm: repression of genes required for the initiation of endosperm development, organization of the endosperm anterior–posterior axis, and the number of divisions of the endosperm nuclei [15•]. In addition, embryos inheriting a maternal copy of fis1 or fis2 rarely de ...
... functions in the developing endosperm: repression of genes required for the initiation of endosperm development, organization of the endosperm anterior–posterior axis, and the number of divisions of the endosperm nuclei [15•]. In addition, embryos inheriting a maternal copy of fis1 or fis2 rarely de ...
FREE FULL TEXT PDF
... roots due to both decreased cell production and mature cell length (West et al., 2004), indicating severe disruption of mitotic activity. Therefore we used a transgenic line (Designated A1) containing a CYCB1::GUS construct as a reporter line for mitotic activity to analyze the effect of ABA on cell ...
... roots due to both decreased cell production and mature cell length (West et al., 2004), indicating severe disruption of mitotic activity. Therefore we used a transgenic line (Designated A1) containing a CYCB1::GUS construct as a reporter line for mitotic activity to analyze the effect of ABA on cell ...
Student Investigations
... In a research paper, choose an animal and compare its chromosome number beyond what was taught. to humans and predict why there is a difference. I can: Make predictions about how various genetic changes can influence generations. I can do everything at a 3.0, and I can demonstrate partial success at ...
... In a research paper, choose an animal and compare its chromosome number beyond what was taught. to humans and predict why there is a difference. I can: Make predictions about how various genetic changes can influence generations. I can do everything at a 3.0, and I can demonstrate partial success at ...
Assembly in G1 phase and long-term stability are unique
... of localization of the same protein synthesized and deposited into chromatin at different times during the cell cycle. Our H3.1-SNAP cell line therefore provides a powerful and accessible tool for marking S-phase progression without the need for an inducible expression system. In contrast, H3.3 (Ray ...
... of localization of the same protein synthesized and deposited into chromatin at different times during the cell cycle. Our H3.1-SNAP cell line therefore provides a powerful and accessible tool for marking S-phase progression without the need for an inducible expression system. In contrast, H3.3 (Ray ...
Comparative Analysis of Paired-and Homeodomain
... fusion protein has been well documented through a series of ectopic PAX3-FKHR expression studies in different cell culture systems [5-9]. More recently, a conditional knock-in strategy targeting PAX3-FKHR expression in differentiated muscle cells demonstrates that PAX3-FKHR plays an essential role i ...
... fusion protein has been well documented through a series of ectopic PAX3-FKHR expression studies in different cell culture systems [5-9]. More recently, a conditional knock-in strategy targeting PAX3-FKHR expression in differentiated muscle cells demonstrates that PAX3-FKHR plays an essential role i ...
Information homeostasis as a fundamental principle governing the
... population of cells. An optimal strategy, according to the model, to control cancer would be to match the composition of nucleotide and amino-acid pools so that genetic information can be expressed with acceptable error rate. Indications of onset of cancer Based on our hypothesis we suggest that fol ...
... population of cells. An optimal strategy, according to the model, to control cancer would be to match the composition of nucleotide and amino-acid pools so that genetic information can be expressed with acceptable error rate. Indications of onset of cancer Based on our hypothesis we suggest that fol ...
June BI5
... A third gene controls the production of the pigment melanin. Mice which are homozygous for the recessive allele, m, of this gene have white fur and pink eyes. They are said to be albino. (i) ...
... A third gene controls the production of the pigment melanin. Mice which are homozygous for the recessive allele, m, of this gene have white fur and pink eyes. They are said to be albino. (i) ...
Tracheary Element Differentiation
... in the Arabidopsis stem add complexity to the data analysis, a point illustrated by the observation that the expression of more than 5000 genes, around 20% of the genome, is altered during stem development (28). However, detailed bioinformatic analysis has identified candidates for many aspects of xy ...
... in the Arabidopsis stem add complexity to the data analysis, a point illustrated by the observation that the expression of more than 5000 genes, around 20% of the genome, is altered during stem development (28). However, detailed bioinformatic analysis has identified candidates for many aspects of xy ...
Introduction to yeast genetics
... 3. If these genes are essential for cell cycle progression, how will we pick mutants if they are all dead? 4. How do we know which genes have the mutations? ...
... 3. If these genes are essential for cell cycle progression, how will we pick mutants if they are all dead? 4. How do we know which genes have the mutations? ...
Matt Lordo STEP Proposal 2013-2014 Personal Statement My name
... observed, it will be concluded that the CM is affecting PTEN at the transcriptional level. If there is a decrease in protein but not at the mRNA level, it will be concluded that factors in the CM are destabilizing PTEN at the protein level. Following analysis of the conditioned media samples, the ep ...
... observed, it will be concluded that the CM is affecting PTEN at the transcriptional level. If there is a decrease in protein but not at the mRNA level, it will be concluded that factors in the CM are destabilizing PTEN at the protein level. Following analysis of the conditioned media samples, the ep ...
Gene Therapy Deffination Is one of the applications of genetic
... cause to disrupt the gene inhibitor of tumor suppressor gene to unleash the oncogene and the damage the latter are more likely than The first and possibly damage the latter is the possibility that the working gene is given in the cells of other non-target cells, which is caused by the bad consequenc ...
... cause to disrupt the gene inhibitor of tumor suppressor gene to unleash the oncogene and the damage the latter are more likely than The first and possibly damage the latter is the possibility that the working gene is given in the cells of other non-target cells, which is caused by the bad consequenc ...
Regulated Lentiviral Packaging Cell Line Devoid of Most Viral cis
... HIV-1 packaging cell line capable of expressing all of the HIV-1 structural, enzymatic, and regulatory proteins but lacking majority of the cis-acting sequences. The use of an inducible expression system circumvented the cytotoxic and cytostatic effects associated with the expression of some of the ...
... HIV-1 packaging cell line capable of expressing all of the HIV-1 structural, enzymatic, and regulatory proteins but lacking majority of the cis-acting sequences. The use of an inducible expression system circumvented the cytotoxic and cytostatic effects associated with the expression of some of the ...
Components of the transcriptional Mediator complex
... mutants, the polarity of the division is reversed (Herman and Horvitz, 1994). It has been proposed that the LIN-44 signal, which acts through the LIN-17 receptor, provides polarity to cells that undergo asymmetric division (Sawa et al., 1996). The Wnt pathway, which controls the polarity of the T ce ...
... mutants, the polarity of the division is reversed (Herman and Horvitz, 1994). It has been proposed that the LIN-44 signal, which acts through the LIN-17 receptor, provides polarity to cells that undergo asymmetric division (Sawa et al., 1996). The Wnt pathway, which controls the polarity of the T ce ...