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NSCLC with high PD-L1 expression on tumor cells or tumor
NSCLC with high PD-L1 expression on tumor cells or tumor

... Background: Programmed death-ligand 1 (PD-L1) expression on tumor cells (TC) or tumor-infiltrating immune cells (IC) is associated with OS, PFS and ORR in pts with advanced NSCLC treated with atezolizumab (anti-PDL1, MPDL3280A; Spigel et al, Spira et al, ASCO 2015), indicating that PD-L1 expression ...
Name: Block: ______ Chapter 2 Cell Growth and Reproduction
Name: Block: ______ Chapter 2 Cell Growth and Reproduction

... posterior will not grow a new anterior. This tells us that the earthworm is capable of ____________________ but not reproduction by ____________________. 40. If you left a sandwich in your locker over the weekend, by Monday, the bread would be covered with a fuzzy, black material that, if touched, w ...
Mechanism of Induction: Induced Pluripotent Stem Cells (iPSCs)
Mechanism of Induction: Induced Pluripotent Stem Cells (iPSCs)

... diseases. A large number of reports have established both scope and potential of Embryonic Stem Cells (ESCs) which are pluripotent cells, which can give rise to all three germ layers: ectoderm, mesoderm, and endoderm and can be used to regenerate the damaged tissues or specific cell lineage. However ...
Using real time RT-PCR analysis to determine multiple gene
Using real time RT-PCR analysis to determine multiple gene

... 2004 very little is known about the molecular pathways regulating gonad development for the limited number of genes that have been identified. As the list of genes increases, studying their interactions will become even more daunting. One approach to investigate gene interactions in genital ridge de ...
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Slides - Workforce Development in Stem Cell Research

... 5. The genetic code uses groups of three bases (CCG, GUU) to encode each amino acid of a protein chain 6. Groups of three bases specify unique amino acids ...
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... G1: the cell is most active metabolically, growing and building proteins appropriate for that cell. Cell may be “arrested” in this stage and not divide again (neurons, muscle). If so, it is more appropriately said that the cell has entered the G0 stage. The cell also ‘proof-reads’ and repairs DNA du ...
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Drosophila Embryonic Cell-Cycle Mutants

... divisions in most embryonic cells, the postblastoderm divisions. At this developmental transition, the cells of the epidermis exit the cell cycle, cells in larval tissues enter the endocycle (an S-G cycle that increases ploidy), whereas mitotic divisions continue in the nervous system. During larval ...
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Plasmids - canesbio

... Studying the Expression of Interacting Groups of Genes Automation has allowed scientists to measure expression of thousands of genes at one time using DNA microarray assays. DNA microarray assays compare patterns of gene expression in different tissues, at different times, or under different condit ...
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... • the cytoplasm of the cell is divided in half and the cell membrane grows to enclose each cell, forming two separate cells as a result. • The end result of mitosis and cytokinesis is two genetically identical cells where only one cell ...
Lin, R., C. D. Allis and S. J. Elledge. 1996. PAT1
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... the yeast histone H1 homologue or other genes that are suppressible by chicken H1 protein. Cells with lethal mutations in the desired gene are unable to grow in the absence of the plasmid carrying chH1 and can be identified by screening for mutants that are unable to lose pMW29H1 in the absence of n ...
Cell division and inheritance
Cell division and inheritance

... Candidates should develop an understanding of the relationship from the molecular level upwards between genes, chromosomes, nuclei and cells and relate these to tissues, organs and systems. For Foundation Tier, knowledge of meiosis is restricted to where the process occurs and that gametes are produ ...
Retinoid-induced apoptosis in normal and neoplastic tissues
Retinoid-induced apoptosis in normal and neoplastic tissues

... positive transcription factors. In addition to their direct effects on transcription, ligand-activated RARs modulate the activity of other transcription factors such as AP-1 (this is termed cross-coupling) (Schule et al, 1991). Activated RARs inhibit the activity of the transcription factors and the ...
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... more similar to the hESCs than to the mESCs. Despite a great potential of differentiation both in vitro with embryoid bodies (EB) and in vivo with teratoma formation, these EpiSCs are unable to form chimeras either through direct injection into blastocysts or using the morula aggregation protocol. M ...
Drosophila Embryonic Cell Cycle Mutants
Drosophila Embryonic Cell Cycle Mutants

... the  embryonic  mitotic  divisions  in  these  mutants.    Because  larval  tissues  grow  by   increasing  ploidy,  and  thus  cell  size,  cell  division  defects  in  the  imaginal  tissues  are   not  manifested  until  the  imaginal ...
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2.0 IN VITRO 2.1

... constructs have also been transfected with a plasmid coding for ...
Genetic alterations and DNA repair in human carcinogenesis
Genetic alterations and DNA repair in human carcinogenesis

... lead to characteristic patterns of mutations. These “genotoxic,” direct-acting carcinogens form covalent adducts with DNA, which cause mutations during DNA replication. The link between mutagenesis and carcinogenesis is also supported by the observation that DNA repair defects are associated with an ...
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Epigenetics in stem-cell differentiation

Embryonic stem cells are capable of self-renewing and differentiating to the desired fate depending on its position within the body. Stem cell homeostasis is maintained through epigenetic mechanisms that are highly dynamic in regulating the chromatin structure as well as specific gene transcription programs. Epigenetics has been used to refer to changes in gene expression, which are heritable through modifications not affecting the DNA sequence.The mammalian epigenome undergoes global remodeling during early stem cell development that requires commitment of cells to be restricted to the desired lineage. There has been multiple evidence suggesting that the maintenance of the lineage commitment of stem cells are controlled by epigenetic mechanisms such as DNA methylation, histone modifications and regulation of ATP-dependent remolding of chromatin structure. Based on the histone code hypothesis, distinct covalent histone modifications can lead to functionally distinct chromatin structures that influence the fate of the cell.This regulation of chromatin through epigenetic modifications is a molecular mechanism that will determine whether the cell will continue to differentiate into the desired fate. A research study performed by Lee et al. examined the effects of epigenetic modifications on the chromatin structure and the modulation of these epigenetic markers during stem cell differentiation through in vitro differentiation of murine embryonic stem (ES) cells.
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