Deep Insight Section Genomic Imprinting: Parental differentiation of the genome
... parental lineage long after pronuclear fusion in the zygote, while the majority of alleles "forget" from which parent they were inherited. This entails dissecting the unique physical chromatin structure and epigenetic DNA modifications, as well as the enzymatic processes that propagate them. The rel ...
... parental lineage long after pronuclear fusion in the zygote, while the majority of alleles "forget" from which parent they were inherited. This entails dissecting the unique physical chromatin structure and epigenetic DNA modifications, as well as the enzymatic processes that propagate them. The rel ...
SICKLE CELL ANEMIA
... and swimming in non-heated water for prolonged periods. Successful treatment of painful episodes include the use of acetaminophen or a nonsteroidal agent early in the course of pain, followed by acetaminophen with codeine and short- or long-acting oral opioids, or hospitalization with IV administrat ...
... and swimming in non-heated water for prolonged periods. Successful treatment of painful episodes include the use of acetaminophen or a nonsteroidal agent early in the course of pain, followed by acetaminophen with codeine and short- or long-acting oral opioids, or hospitalization with IV administrat ...
Lineage-specific Gene Expression in the Sea
... can be traced back to particular sets of early blastomeres. Classical cell lineage and experimental studies (H6rstadius 1939; for review, see Angerer and Davidson 1984) have shown that certain of these lineages appear to be specified, at least in part, in consequence of the maternal components inher ...
... can be traced back to particular sets of early blastomeres. Classical cell lineage and experimental studies (H6rstadius 1939; for review, see Angerer and Davidson 1984) have shown that certain of these lineages appear to be specified, at least in part, in consequence of the maternal components inher ...
CHAPTER THREE CYCLIN TRANSFORMATION OF BANANA
... Plasmid DNA was transferred into E. coli (strain JM 109) using the heat shock method. The procedure involved placing 100 µl of the competent bacteria cells from -80oC storage and thawing them on ice for 30 min. One microgram of plasmid DNA was added to the bacteria cells, gently mixed and left to st ...
... Plasmid DNA was transferred into E. coli (strain JM 109) using the heat shock method. The procedure involved placing 100 µl of the competent bacteria cells from -80oC storage and thawing them on ice for 30 min. One microgram of plasmid DNA was added to the bacteria cells, gently mixed and left to st ...
Promoter-trapping in Saccharomyces cerevisiae
... in a variety of organisms (17). To avoid a sequence bias towards genomic recognition sites of the co-transformed endonuclease, we here investigate whether g-irradiation is suitable for facilitating NHI. Within the yeast genome, DSB induced by sparsely ionising radiation appear to be distributed rand ...
... in a variety of organisms (17). To avoid a sequence bias towards genomic recognition sites of the co-transformed endonuclease, we here investigate whether g-irradiation is suitable for facilitating NHI. Within the yeast genome, DSB induced by sparsely ionising radiation appear to be distributed rand ...
Analysis of cell division parameters and cell cycle gene expression
... cycle phase durations. Subsequently, this model system was used to follow the transcription profile of key cell cycle genes during a complete cultivation cycle. According to the calculated changes in the relative division rate over time, the cell cycle genes could be classified into four groups base ...
... cycle phase durations. Subsequently, this model system was used to follow the transcription profile of key cell cycle genes during a complete cultivation cycle. According to the calculated changes in the relative division rate over time, the cell cycle genes could be classified into four groups base ...
Suppression of the Malignant Phenotype in Somatic Cell Hybrids
... Tumorigenicity in Nude Mice. The parental cell lines (BL60P7, IARC 277) and the hybrid clones were tested for their tumorigenic potential in nude mice by inoculation of 1 x IO7 cells into each flank of preirradiated nude mice (29). No differ ence was seen between the in vivo growth pattern of the hy ...
... Tumorigenicity in Nude Mice. The parental cell lines (BL60P7, IARC 277) and the hybrid clones were tested for their tumorigenic potential in nude mice by inoculation of 1 x IO7 cells into each flank of preirradiated nude mice (29). No differ ence was seen between the in vivo growth pattern of the hy ...
Epigenetic mechanisms regulate placental c-myc
... sequences (E-boxes) via its bHLH domain recruiting histone acetyltransferases and further, dimerizing with its partner Max, with its leucine zipper domain, thus regulating the expression of a large number of downstream target genes (Gearhart et al., 2007) involved in cell growth and proliferation. O ...
... sequences (E-boxes) via its bHLH domain recruiting histone acetyltransferases and further, dimerizing with its partner Max, with its leucine zipper domain, thus regulating the expression of a large number of downstream target genes (Gearhart et al., 2007) involved in cell growth and proliferation. O ...
... software tools, methods, and standards for flow cytometry, and include bioinformaticists, computational statisticians, software and hardware developers and clinician scientists, from both academia and industry (details of the FICCS meeting on page 5). Contact Ryan Brinkman at [email protected] for more ...
Chapter 20 PPT
... incompatibility issues by using eukaryotic cells, such as yeasts, as hosts for cloning and expressing genes • Even yeasts may not possess the proteins required to modify expressed mammalian proteins properly • In such cases, cultured mammalian or insect cells may be used to express and study protein ...
... incompatibility issues by using eukaryotic cells, such as yeasts, as hosts for cloning and expressing genes • Even yeasts may not possess the proteins required to modify expressed mammalian proteins properly • In such cases, cultured mammalian or insect cells may be used to express and study protein ...
DNA Methylation Maintains Allele-specific KIR Gene Expression in
... To investigate expression of other KIR alleles, we further examined donor K, who is heterozygous at the 3DL1, 3DL2, and 2DL4 loci. After amplification with gene-specific primers, bulk RT-PCR products were sequenced. The presence of one or two signals at polymorphic sites indicated monoallelic and b ...
... To investigate expression of other KIR alleles, we further examined donor K, who is heterozygous at the 3DL1, 3DL2, and 2DL4 loci. After amplification with gene-specific primers, bulk RT-PCR products were sequenced. The presence of one or two signals at polymorphic sites indicated monoallelic and b ...
International Cell Line Authentication Committee ICLAC Guide to
... specify suppliers. Suppliers can be found online (e.g., by entering “cell line str profiling” or “cell line authentication” into your search engine). It is essential that the supplier chosen provides a rapid and reliable service with appropriate accreditation and quality assurance. The supplier shou ...
... specify suppliers. Suppliers can be found online (e.g., by entering “cell line str profiling” or “cell line authentication” into your search engine). It is essential that the supplier chosen provides a rapid and reliable service with appropriate accreditation and quality assurance. The supplier shou ...
Paneth cell phenotype is associated with clinical outcome
... NOD2 risk variants may be associated with an abnormal granule phenotype in the Paneth cells of CD patients ...
... NOD2 risk variants may be associated with an abnormal granule phenotype in the Paneth cells of CD patients ...
Argonaute2 Is Essential for Mammalian
... Gastrulation is a developmental phase that delineates the three embryonic germ layers: ectoderm, endoderm, and mesoderm. The gene Brachyury is essential for mesoderm development, and shorttail mice, which were later found to be carrying a Brachyury mutation, have been known since 1927. In this study ...
... Gastrulation is a developmental phase that delineates the three embryonic germ layers: ectoderm, endoderm, and mesoderm. The gene Brachyury is essential for mesoderm development, and shorttail mice, which were later found to be carrying a Brachyury mutation, have been known since 1927. In this study ...
- Horizon Discovery
... NanoLuc® luciferase is a novel, small, bright luciferase reporter. It is one third the size and 100 times brighter than the most commonly used luciferase, firefly. These features enable the development of homogenous assays for primary and secondary screening, reporting on pathways via the endogenous ...
... NanoLuc® luciferase is a novel, small, bright luciferase reporter. It is one third the size and 100 times brighter than the most commonly used luciferase, firefly. These features enable the development of homogenous assays for primary and secondary screening, reporting on pathways via the endogenous ...
University of Birmingham Immunolabelling of human metaphase
... Our previously published work described how immunofluorescence microscopy could be used to provide an overview of the distribution of histone modifications across human metaphase chromosomes. Using metaphase chromosome spreads from lymphoblastoid cell lines (LCL) of normal karyotype and antisera to ...
... Our previously published work described how immunofluorescence microscopy could be used to provide an overview of the distribution of histone modifications across human metaphase chromosomes. Using metaphase chromosome spreads from lymphoblastoid cell lines (LCL) of normal karyotype and antisera to ...
DNA Structure: Gumdrop Modeling Student Advanced Version
... This packaging not only helps the DNA to fit into the nucleus, it also helps to control which parts of the DNA are expressed, or turned on. Think about it – a blood cell and a muscle cell look very different, and have very different jobs to do (see the pictures below), but they have the exact same D ...
... This packaging not only helps the DNA to fit into the nucleus, it also helps to control which parts of the DNA are expressed, or turned on. Think about it – a blood cell and a muscle cell look very different, and have very different jobs to do (see the pictures below), but they have the exact same D ...
Olivier Pourquie. 2003. The Segmentation Clock: Converting
... natic Fringe (5, 6) and the Notch ligand deltaC (9). Their cycling behavior in the PSM is regulated at the transcriptional level (13, 14). All these genes oscillate largely in synchrony in the PSM, suggesting that they are downstream of a common cycling activator. The mechanism driving the oscillati ...
... natic Fringe (5, 6) and the Notch ligand deltaC (9). Their cycling behavior in the PSM is regulated at the transcriptional level (13, 14). All these genes oscillate largely in synchrony in the PSM, suggesting that they are downstream of a common cycling activator. The mechanism driving the oscillati ...
The role of the SRY gene in determing sex.
... The patients DNA and control samples were tested by PCR(Polymerase chain reaction)amplification in figure A. Figure B shows fluorescent microscopy on the patients DNA. Fluorescence in situ hybridsation of patients DNA showed a green fluorescence on the short arm of the X chromosome. This gen was tra ...
... The patients DNA and control samples were tested by PCR(Polymerase chain reaction)amplification in figure A. Figure B shows fluorescent microscopy on the patients DNA. Fluorescence in situ hybridsation of patients DNA showed a green fluorescence on the short arm of the X chromosome. This gen was tra ...
Sex Chromosomes
... Xist (X inactivation specific transcript) • A large (17kb in human) untranslated RNA transcript in the nucleus • Exclusively expressed from the inactive X • Coats the inactive X in somatic cells of females • Required in cis for the initiation of X inactivation • Contains a short repeat (RepA) at it ...
... Xist (X inactivation specific transcript) • A large (17kb in human) untranslated RNA transcript in the nucleus • Exclusively expressed from the inactive X • Coats the inactive X in somatic cells of females • Required in cis for the initiation of X inactivation • Contains a short repeat (RepA) at it ...
Birth of fertile bimaternal offspring following
... not H19-DMR deletion, was suffcient to produce fullterm bimaternal pups, indicating that the Dlk1-Dio3 region has a more profound effect on fetal growth. Though both the 15 kb and 5 kb regions contained the ICR of the H19-Igf2 cluster, only the 15 kb deletion could reactivate Igf2 expression and sup ...
... not H19-DMR deletion, was suffcient to produce fullterm bimaternal pups, indicating that the Dlk1-Dio3 region has a more profound effect on fetal growth. Though both the 15 kb and 5 kb regions contained the ICR of the H19-Igf2 cluster, only the 15 kb deletion could reactivate Igf2 expression and sup ...
DNA
... • To overcome differences in promoters and other DNA control sequences, scientists usually employ an expression vector, a cloning vector that contains a highly active bacterial promoter 表現載體使用適當的細菌啟動子 ...
... • To overcome differences in promoters and other DNA control sequences, scientists usually employ an expression vector, a cloning vector that contains a highly active bacterial promoter 表現載體使用適當的細菌啟動子 ...
Transient cold shock enhances zinc-finger nuclease
... be broadly applied to increase the cellular activity of a ZFN pair across a variety of experimental settings would facilitate application of this promising technology. To this end, much effort has been devoted to developing general strategies for improving ZFN function, including the design of new i ...
... be broadly applied to increase the cellular activity of a ZFN pair across a variety of experimental settings would facilitate application of this promising technology. To this end, much effort has been devoted to developing general strategies for improving ZFN function, including the design of new i ...
The ROOT HAIRLESS 1 gene encodes a nuclear protein
... The epidermis of Arabidopsis wild-type primary roots, in which some cells grow hairs and others remain hairless in a position-dependent manner, has become an established model system to study cell differentiation. Here we present a molecular analysis of the RHL1 (ROOT HAIRLESS 1) gene that, if mutat ...
... The epidermis of Arabidopsis wild-type primary roots, in which some cells grow hairs and others remain hairless in a position-dependent manner, has become an established model system to study cell differentiation. Here we present a molecular analysis of the RHL1 (ROOT HAIRLESS 1) gene that, if mutat ...
blumberg-lab.bio.uci.edu
... nucleosome remodeling ● Contains key protein in chromosome structure ● Genes are linked to behavior and tissue specific processes ...
... nucleosome remodeling ● Contains key protein in chromosome structure ● Genes are linked to behavior and tissue specific processes ...