Table S3 Compounds in the isogenic cell line high throughput drug
... Intraperitoneal (IP) and oral dasatinib treatment at a range of concentrations cause target inhibition (reduction in phosphorylated-SRC). Animals were treated with varying doses of dasatinib by either oral gavage or IP injection for 72 hours. Tumour and normal tissue was then collected and lysates m ...
... Intraperitoneal (IP) and oral dasatinib treatment at a range of concentrations cause target inhibition (reduction in phosphorylated-SRC). Animals were treated with varying doses of dasatinib by either oral gavage or IP injection for 72 hours. Tumour and normal tissue was then collected and lysates m ...
Document
... Epigenetic machinery was established by Life for ‘genome protection’ purposes first. In the further course of evolution, however, the mechanisms of processing of epigenetic information - which allow recognition of different epigenetic marks and channeling the signals encoded in these marks along app ...
... Epigenetic machinery was established by Life for ‘genome protection’ purposes first. In the further course of evolution, however, the mechanisms of processing of epigenetic information - which allow recognition of different epigenetic marks and channeling the signals encoded in these marks along app ...
Implications of DNA replication for eukaryotic gene expression
... in a transcriptionally active state are now clear (Fig. 1; and Brown, 1984; Mitchell and Tjian, 1989). The initial direct binding of transcription factors to DNA is rapid, the sequestration of non-DNA binding factors is relatively slow. In vitro, the process of assembling a complete transcription co ...
... in a transcriptionally active state are now clear (Fig. 1; and Brown, 1984; Mitchell and Tjian, 1989). The initial direct binding of transcription factors to DNA is rapid, the sequestration of non-DNA binding factors is relatively slow. In vitro, the process of assembling a complete transcription co ...
DNA methylation profiling identifies epigenetic dysregulation in
... in CGI shores (Doi et al, 2009; Irizarry et al, 2009). We used bioinformatic tools to determine the distance of differentially methylated CpGs to the nearest CGI. Utilising CGI prediction (Bock et al, 2007), we found that B50% of the differentially methylated CpG sites are located 42 kb from the nea ...
... in CGI shores (Doi et al, 2009; Irizarry et al, 2009). We used bioinformatic tools to determine the distance of differentially methylated CpGs to the nearest CGI. Utilising CGI prediction (Bock et al, 2007), we found that B50% of the differentially methylated CpG sites are located 42 kb from the nea ...
The Modular Structure and Function of the Wheat HI Promoter with S
... TH315 protein with other plant His showed that similarity was rather restricted to the central globular domain (Fig. 3A), ranging from 80% for wcHl-1 (Yang et al. 1991) to 50% for Arabidopsis Hisl-3 (Ascenzi and Gantt 1997). In a phylogenetic tree, plant HI proteins can be classified into at least t ...
... TH315 protein with other plant His showed that similarity was rather restricted to the central globular domain (Fig. 3A), ranging from 80% for wcHl-1 (Yang et al. 1991) to 50% for Arabidopsis Hisl-3 (Ascenzi and Gantt 1997). In a phylogenetic tree, plant HI proteins can be classified into at least t ...
Genome engineering of mammalian haploid embryonic stem cells
... promoter (Mali et al., 2013). To examine the efficiency of knockout of each gene, haploid ESCs were co-transfected with the Cas9 expression vector and a sgRNA vector targeting Tet1, Tet2 orTet3, along with a puromycin marker. Transfected cells were treated with 1 µg/ml puromycin for 48 h. Each targe ...
... promoter (Mali et al., 2013). To examine the efficiency of knockout of each gene, haploid ESCs were co-transfected with the Cas9 expression vector and a sgRNA vector targeting Tet1, Tet2 orTet3, along with a puromycin marker. Transfected cells were treated with 1 µg/ml puromycin for 48 h. Each targe ...
How different is anatomy?
... None of the model organism databases used standard terminology to describe biological function Drosophila sequence was imminent Largest genome sequenced at that time Two weeks, 3 dozen scientists, all new software How could we organize the annotation? ...
... None of the model organism databases used standard terminology to describe biological function Drosophila sequence was imminent Largest genome sequenced at that time Two weeks, 3 dozen scientists, all new software How could we organize the annotation? ...
神经发生的分子机制（景乃禾）
... FGFs are not acting as direct neural inducers in the early postimplantation mouse embryo. ...
... FGFs are not acting as direct neural inducers in the early postimplantation mouse embryo. ...
Osteoclasts
... DC-STAMP (Dcst) and cathepsin K (CtsK). In addition, osteoclast-specific Jmjd5 KO mice were generated by crossing Jmjd5-flox mice with CtsK-Cre mice; reduced bone mass in the femurs of these KO mice (16-week-old) was observed, as compared with WT mice. These results clearly indicate that epigenetic ...
... DC-STAMP (Dcst) and cathepsin K (CtsK). In addition, osteoclast-specific Jmjd5 KO mice were generated by crossing Jmjd5-flox mice with CtsK-Cre mice; reduced bone mass in the femurs of these KO mice (16-week-old) was observed, as compared with WT mice. These results clearly indicate that epigenetic ...
Modeling Fragile X Syndrome Using Human Pluripotent Stem Cells
... expansion with an active FMR1 gene, it recurrently resulted in hypermethylation and complete inactivation of FMR1 in the newly established iPSC clones [34]. This led to the conclusion that transcription factor reprogramming may incorrectly hypermethylate this region provided that the CGGs are suffic ...
... expansion with an active FMR1 gene, it recurrently resulted in hypermethylation and complete inactivation of FMR1 in the newly established iPSC clones [34]. This led to the conclusion that transcription factor reprogramming may incorrectly hypermethylate this region provided that the CGGs are suffic ...
activator
... • Chemical modifications to histones and DNA of chromatin influence both chromatin structure and gene expression ...
... • Chemical modifications to histones and DNA of chromatin influence both chromatin structure and gene expression ...
Gene Section RASSF6 (Ras association (RalGDS/AF-6) domain family member 6)
... demonstrates that Hippo is present in Drosophila cells in two pools; an active form associated with Salvador and an inactive form associated with dRASSF (Polesello et al., 2006). Furthermore, RNAi-mediated dRASSF depletion led to a marked increase in Hippo activation following Staurosporine (STS) tr ...
... demonstrates that Hippo is present in Drosophila cells in two pools; an active form associated with Salvador and an inactive form associated with dRASSF (Polesello et al., 2006). Furthermore, RNAi-mediated dRASSF depletion led to a marked increase in Hippo activation following Staurosporine (STS) tr ...
The possibilities of practical application of transgenic mammalian
... cell-descended genes. The process of epigenomically dependent reprogramming is related to the stable erasure (“vanishing”) of donor cell nuclear DNA-epigenetic status and turning back (“molecular zeroing/nulling”) the somatogenic “transcriptional and translational clock”. This contributes to recapit ...
... cell-descended genes. The process of epigenomically dependent reprogramming is related to the stable erasure (“vanishing”) of donor cell nuclear DNA-epigenetic status and turning back (“molecular zeroing/nulling”) the somatogenic “transcriptional and translational clock”. This contributes to recapit ...
Maintenance of DNA Methylation during the Arabidopsis Life Cycle
... methylation. However, it is still not clear to what extent similar cis-acting elements may be part of a conserved molecular mechanism controlling maternally imprinted genes. In this work, we show that the Polycomb group gene FERTILIZATIONINDEPENDENT SEED2 (FIS2) is imprinted. Maintenance of FIS2 imp ...
... methylation. However, it is still not clear to what extent similar cis-acting elements may be part of a conserved molecular mechanism controlling maternally imprinted genes. In this work, we show that the Polycomb group gene FERTILIZATIONINDEPENDENT SEED2 (FIS2) is imprinted. Maintenance of FIS2 imp ...
Work Force Development: Stem Cell Primer - Bio-Link
... obtained from multiple sources. Embryonic-like cells can be generated from morula cells (a pre-blastula embryo of 8 cells), from naturally-occurring tumors like teratomas (so-called “embryonal carcinoma” or EC cells) or from primordial germ cells (which are termed “embryonic germ” or EG cells). Embr ...
... obtained from multiple sources. Embryonic-like cells can be generated from morula cells (a pre-blastula embryo of 8 cells), from naturally-occurring tumors like teratomas (so-called “embryonal carcinoma” or EC cells) or from primordial germ cells (which are termed “embryonic germ” or EG cells). Embr ...
Differentiation of plant cells during symbiotic nitrogen fixation
... nodulins have been shown to be expressed in other plant tissues when assayed with more sensitive expression analysis techniques such as RT-PCR (e.g. [50]). In this respect the original nodulin concept is over-simplistic; in reality there are probably few genes that are expressed exclusively in nodul ...
... nodulins have been shown to be expressed in other plant tissues when assayed with more sensitive expression analysis techniques such as RT-PCR (e.g. [50]). In this respect the original nodulin concept is over-simplistic; in reality there are probably few genes that are expressed exclusively in nodul ...
MicroRNAs act sequentially and asymmetrically to
... homeobox gene expression • Correct lim-6 expression requires a regulated balance of the ceh-36 activator homeobox gene and the cog-1 repressor gene. • Loss of lim-6 could either mean an increase in expression of the ceh-36 activator or a decrease in expression of the cog-1 repressor. • loss of die-1 ...
... homeobox gene expression • Correct lim-6 expression requires a regulated balance of the ceh-36 activator homeobox gene and the cog-1 repressor gene. • Loss of lim-6 could either mean an increase in expression of the ceh-36 activator or a decrease in expression of the cog-1 repressor. • loss of die-1 ...
The Effects of Plasmid on Genotype and Phenotype
... you can readily appreciate how this type of gene can cause serious medical problems when it occurs in pathogenic bacteria. For this reason, the plasmids such as pUC 18 which are used in recombinant DNA experiments were designed so that they cannot be exchanged with other bacteria except by special t ...
... you can readily appreciate how this type of gene can cause serious medical problems when it occurs in pathogenic bacteria. For this reason, the plasmids such as pUC 18 which are used in recombinant DNA experiments were designed so that they cannot be exchanged with other bacteria except by special t ...
The appearance of secondary cell walls was a - Plant-o
... A perplexing biopolymer: how autofluorescence is uncovering clues to sporopollenin composition and assembly Teagen D. Quilichini, A. Lacey Samuels, and Carl J. Douglas Department of Botany, University of British Columbia, Vancouver, Canada The highly resistant biopolymer, sporopollenin, gives the ou ...
... A perplexing biopolymer: how autofluorescence is uncovering clues to sporopollenin composition and assembly Teagen D. Quilichini, A. Lacey Samuels, and Carl J. Douglas Department of Botany, University of British Columbia, Vancouver, Canada The highly resistant biopolymer, sporopollenin, gives the ou ...
Protocol
... chemical synthesis (for siRNA) and vector-based expression (for shRNA) [8-12]. While effective in triggering RNAi, the synthetic siRNAs are expensive and only mediate transient knockdown effect. In contrast, the promoter driven expression of short hairpin RNAs (shRNAs) in cells is more cost-effectiv ...
... chemical synthesis (for siRNA) and vector-based expression (for shRNA) [8-12]. While effective in triggering RNAi, the synthetic siRNAs are expensive and only mediate transient knockdown effect. In contrast, the promoter driven expression of short hairpin RNAs (shRNAs) in cells is more cost-effectiv ...
The molecular biology of sex determination and sexual development
... Our research examines the molecular events underlying the development of sex in birds. We wish to learn more about the mechanism of sex determination in birds also find markers that will assist in the early identification of avian sex. The chicken is a very suitable model for scientific study becaus ...
... Our research examines the molecular events underlying the development of sex in birds. We wish to learn more about the mechanism of sex determination in birds also find markers that will assist in the early identification of avian sex. The chicken is a very suitable model for scientific study becaus ...
General Pathology
... Oncogenic RNA cancers in humens. Human T-cell leukemia virus type 1 (HTLV-1) . causes a form of T-cell leukemia/lymphoma. In addition there is onchogenic DNA virus.,helicobacter pylori. 3)Age Age has an important influence on the likehood of being affected with cancer.Most of the cases occur after t ...
... Oncogenic RNA cancers in humens. Human T-cell leukemia virus type 1 (HTLV-1) . causes a form of T-cell leukemia/lymphoma. In addition there is onchogenic DNA virus.,helicobacter pylori. 3)Age Age has an important influence on the likehood of being affected with cancer.Most of the cases occur after t ...
Morphine Modulates Mouse Hippocampal Progenitor Cell Lineages
... induce an irreversible switch from NPCs to astrocytes in a way independently of STAT3 [18]. This ability to promote astrogliogenesis was further shown to depend on CSL-mediated glial gene activation [19]. Prospero homeobox protein 1 (Prox1) is a transcription factor necessary for progenitor cell pro ...
... induce an irreversible switch from NPCs to astrocytes in a way independently of STAT3 [18]. This ability to promote astrogliogenesis was further shown to depend on CSL-mediated glial gene activation [19]. Prospero homeobox protein 1 (Prox1) is a transcription factor necessary for progenitor cell pro ...
PDF
... centromere protein (INCENP) and plays a role during both gametophytic differentiation and postfertilization development. Disruption of WYR affects cell fate establishment in the female gametophyte and progression through PMI during pollen development. Consistent with the developmental phenotypes of ...
... centromere protein (INCENP) and plays a role during both gametophytic differentiation and postfertilization development. Disruption of WYR affects cell fate establishment in the female gametophyte and progression through PMI during pollen development. Consistent with the developmental phenotypes of ...
Embryonic Stem Cell Culture Conditions Support Distinct States
... with later stages of development (E4.5), at which point embryonic cells are more restricted in their developmental potential. Thus, ESC culture systems are not equivalent, but support cell types that resemble distinct developmental stages. Cells derived in one condition can be reprogrammed to anothe ...
... with later stages of development (E4.5), at which point embryonic cells are more restricted in their developmental potential. Thus, ESC culture systems are not equivalent, but support cell types that resemble distinct developmental stages. Cells derived in one condition can be reprogrammed to anothe ...