Genome-scale CRISPR pooled screens
... identifying genetic drivers of metastasis in an in vivo screen [20], and understanding gene networks in immune cells [21,22]. In addition, two catalytically inactive versions of Cas9 with arrays of transcriptional activation domains (in conjunction with sgRNA libraries targeting promoter regions) ha ...
... identifying genetic drivers of metastasis in an in vivo screen [20], and understanding gene networks in immune cells [21,22]. In addition, two catalytically inactive versions of Cas9 with arrays of transcriptional activation domains (in conjunction with sgRNA libraries targeting promoter regions) ha ...
Malaysian Guidelines for Stem Cell Research and Therapy
... tract plus various accessory organs like the lungs and liver. Much of the proposed research on stem cells centers upon the early human embryo. ...
... tract plus various accessory organs like the lungs and liver. Much of the proposed research on stem cells centers upon the early human embryo. ...
Live Cell Luciferase Assay Kit
... intensity quickly (within 10-30 min.), and the immediate measurement of the luminescence signals is recommended. SOLUTION ASSAY One method of assaying the expression of the luc gene in transiently transfected cell lines is by lysis of the recombinant cells and assay of the supernatant cell suspensio ...
... intensity quickly (within 10-30 min.), and the immediate measurement of the luminescence signals is recommended. SOLUTION ASSAY One method of assaying the expression of the luc gene in transiently transfected cell lines is by lysis of the recombinant cells and assay of the supernatant cell suspensio ...
Summary and Discussion English
... of metaphases of human lymphocytes using whole chromosome and band specific DNA probes revealed that chromosome 9 homologues were frequently involved in MMC induced chromatid exchanges and all of these exchanges were formed between the paracentromeric heterochromatic regions (9q12-13). In contrast, ...
... of metaphases of human lymphocytes using whole chromosome and band specific DNA probes revealed that chromosome 9 homologues were frequently involved in MMC induced chromatid exchanges and all of these exchanges were formed between the paracentromeric heterochromatic regions (9q12-13). In contrast, ...
Rare coding SNP in DZIP1 gene associated with late
... Firstly, PD would be defined in terms of the PD-state, a characteristic deviation from the normal expression program of a healthy cell. Singular cellular manifestations of PD would therefore be de-emphasized in favor of this systems-level definition. Crucially, the PD-state would be a generic cell s ...
... Firstly, PD would be defined in terms of the PD-state, a characteristic deviation from the normal expression program of a healthy cell. Singular cellular manifestations of PD would therefore be de-emphasized in favor of this systems-level definition. Crucially, the PD-state would be a generic cell s ...
word
... are selectively discarded from the genome during development. Three types of tissue-specific chromosome elimination events occur in sciarids. During early cleavages, one or two paternal chromosomes is/are discarded from somatic cells of embryos, depending on the sex of the embryo. In early germ cell ...
... are selectively discarded from the genome during development. Three types of tissue-specific chromosome elimination events occur in sciarids. During early cleavages, one or two paternal chromosomes is/are discarded from somatic cells of embryos, depending on the sex of the embryo. In early germ cell ...
On the origin and frequency of Y chromosome deletions responsible
... explain the transmission of an expanded deletion from a fertile father to an infertile son (Kobayashi et al., 1994). Genetic factors regulating chromosomal associations and chiasma formation during meiosis may thus predispose some men to disturbances in the onset and normality of meiotic pairing. In ...
... explain the transmission of an expanded deletion from a fertile father to an infertile son (Kobayashi et al., 1994). Genetic factors regulating chromosomal associations and chiasma formation during meiosis may thus predispose some men to disturbances in the onset and normality of meiotic pairing. In ...
Datasheet - Santa Cruz Biotechnology, Inc.
... The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p5 ...
... The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p5 ...
Li, H., and Baker, B. S.
... necessary for male sexual behavior (Hall, 1994; Ito et al., 1996; Ryner et al., 1996; Taylor et al., 1994) and the development of a male-specific abdominal muscle, the Muscle of Lawrence (MOL) (Gailey et al., 1991; Ito et al., 1996; Lawrence and Johnston, 1986; Ryner et al., 1996). The female-specif ...
... necessary for male sexual behavior (Hall, 1994; Ito et al., 1996; Ryner et al., 1996; Taylor et al., 1994) and the development of a male-specific abdominal muscle, the Muscle of Lawrence (MOL) (Gailey et al., 1991; Ito et al., 1996; Lawrence and Johnston, 1986; Ryner et al., 1996). The female-specif ...
Molecular Mechanisms of Long Noncoding RNAs
... et al., 2009). Kcnq1ot1 is a 90 kb lncRNA expressed from the paternal allele that directs silencing of a cluster of genes in the imprinted Kcnq1 domain (Pandey et al., 2008). Kcnq1ot1 interacts with the histone methyltransferases G9a and PRC2, effectively forming a repression domain in cis to its tr ...
... et al., 2009). Kcnq1ot1 is a 90 kb lncRNA expressed from the paternal allele that directs silencing of a cluster of genes in the imprinted Kcnq1 domain (Pandey et al., 2008). Kcnq1ot1 interacts with the histone methyltransferases G9a and PRC2, effectively forming a repression domain in cis to its tr ...
DNA interference: DNA-induced gene silencing in the
... 5.5 h post-fertilization (hpf) (figure 3a). This phenotype was in accord with the result of RNAi-mediated knockdown of Ziclike1 (figure 3a). To investigate the effect of PCR-AChE (covering 819 bp), the amount of AChE protein was monitored by histochemical staining in 7 hpf larvae. In uninjected cont ...
... 5.5 h post-fertilization (hpf) (figure 3a). This phenotype was in accord with the result of RNAi-mediated knockdown of Ziclike1 (figure 3a). To investigate the effect of PCR-AChE (covering 819 bp), the amount of AChE protein was monitored by histochemical staining in 7 hpf larvae. In uninjected cont ...
De Novo Nonsense Mutations in KAT6A, a Lysine Acetyl
... and Stanley F. Nelson1,10,* Chromatin remodeling through histone acetyltransferase (HAT) and histone deactylase (HDAC) enzymes affects fundamental cellular processes including the cell-cycle, cell differentiation, metabolism, and apoptosis. Nonsense mutations in genes that are involved in histone ac ...
... and Stanley F. Nelson1,10,* Chromatin remodeling through histone acetyltransferase (HAT) and histone deactylase (HDAC) enzymes affects fundamental cellular processes including the cell-cycle, cell differentiation, metabolism, and apoptosis. Nonsense mutations in genes that are involved in histone ac ...
Chromoplast Differentiation: Current Status and
... central core and are surrounded by a layer of polar lipids, which in turn are surrounded by an outer layer of the plastoglobulin fibrillin. In fact, fibrillin, which is highly expressed in ripening fruit, allows the sequestration of lycopene in the form of crystals within membrane structures. The sequ ...
... central core and are surrounded by a layer of polar lipids, which in turn are surrounded by an outer layer of the plastoglobulin fibrillin. In fact, fibrillin, which is highly expressed in ripening fruit, allows the sequestration of lycopene in the form of crystals within membrane structures. The sequ ...
Pax6 lights-up the way for eye development Ruth Ashery
... OV invaginates to form the optic cup (Figure 4c). This step is completely dependent on the development of a lens placode as demonstrated by analysis of the Le-mutant embryos where the loss of Pax6 activity in the SE resulted in genetic ablation of the lens placode (Figure 4c,d). In Le-mutants the op ...
... OV invaginates to form the optic cup (Figure 4c). This step is completely dependent on the development of a lens placode as demonstrated by analysis of the Le-mutant embryos where the loss of Pax6 activity in the SE resulted in genetic ablation of the lens placode (Figure 4c,d). In Le-mutants the op ...
... demonstrated. Genetics, that is the DNA sequence with its individual variants, is inherited from the parents and will remain intact for the whole lifespan: it will participate to the individual variation of the phenotype, thus, to disease causation, through functional variants that are shared by T2D ...
PDF version - EpiGeneSys
... Whilst great insights into the structure and properties of chromatin have been gained using chromatin samples extracted from native sources, analyses of such material have limitations because of their inherent heterogeneity. Native chromatin samples contain an ensemble of different core histones, li ...
... Whilst great insights into the structure and properties of chromatin have been gained using chromatin samples extracted from native sources, analyses of such material have limitations because of their inherent heterogeneity. Native chromatin samples contain an ensemble of different core histones, li ...
Characterization of two rice DNA methyltransferases
... transcription factors that require contact with the major groove of the double helix. Alternatively, cytosine methylation can cause structural changes of chromatin that limit promoter accessibility by transcriptional machinery. The discovery of several methyl binding domain (MBD)containing proteins ...
... transcription factors that require contact with the major groove of the double helix. Alternatively, cytosine methylation can cause structural changes of chromatin that limit promoter accessibility by transcriptional machinery. The discovery of several methyl binding domain (MBD)containing proteins ...
Immunodeficiency Agammaglobulinemia, the First Primary
... would allow a better understanding of the functional significance of the BTK protein domains. More than 800 mutations have been identified that are scattered throughout the BTK gene and include missense, nonsense, and insertion/deletion mutations; however, no straightforward structure–function corre ...
... would allow a better understanding of the functional significance of the BTK protein domains. More than 800 mutations have been identified that are scattered throughout the BTK gene and include missense, nonsense, and insertion/deletion mutations; however, no straightforward structure–function corre ...
Plxdc2 Is a Mitogen for Neural Progenitors Suzanne F. C. Miller-Delaney
... Expression of diffusible molecules controlling both proliferation and cell fate specification from defined organising centres in the developing nervous system underlies the coordination of differentiation and growth of different brain regions. Many important families of secreted molecules involved i ...
... Expression of diffusible molecules controlling both proliferation and cell fate specification from defined organising centres in the developing nervous system underlies the coordination of differentiation and growth of different brain regions. Many important families of secreted molecules involved i ...
Imaging fertilization in flowering plants, not so
... HTR10 does not occur after fertilization of the central cell, suggesting that the two fertilization events are not equivalent in terms of chromatin dynamics (Ingouff et al., 2007). Using the ubiquitously expressed centromeric histone variant H3 (CENH3), in vivo imaging enabled the earliest timing of ...
... HTR10 does not occur after fertilization of the central cell, suggesting that the two fertilization events are not equivalent in terms of chromatin dynamics (Ingouff et al., 2007). Using the ubiquitously expressed centromeric histone variant H3 (CENH3), in vivo imaging enabled the earliest timing of ...
Cell Biology - Assays Kits
... performed visually by microscopy, but this is time consuming and not convenient for numerous samples, nor possible if cells are cultured on support that disagregate (ceramics, HA). Counting is now also performed in automates. Tritiated thimidine method, based on the incorporation of H3-thymidine int ...
... performed visually by microscopy, but this is time consuming and not convenient for numerous samples, nor possible if cells are cultured on support that disagregate (ceramics, HA). Counting is now also performed in automates. Tritiated thimidine method, based on the incorporation of H3-thymidine int ...
Hammond 1 Regulation of gene expression during flocculation in
... vector with cloned promoter regions was also digested with two different restriction enzymes: Xho-1 and EcoR1-HF (New England Biolabs) under conditions specified by the manufacturer (New England Biolabs). This mixture was placed at 37ºC for 1 hour and then analyzed by agarose gel electrophoresis. Th ...
... vector with cloned promoter regions was also digested with two different restriction enzymes: Xho-1 and EcoR1-HF (New England Biolabs) under conditions specified by the manufacturer (New England Biolabs). This mixture was placed at 37ºC for 1 hour and then analyzed by agarose gel electrophoresis. Th ...
1 X chromosome crossover formation and genome stability in
... germ cells in most metazoans also intentionally create double-strand breaks (DSBs) to promote DNA exchange between parental chromosomes, a process known as crossing over. Homologous recombination is employed in the repair of both genotoxic lesions and programmed DSBs and many of the core DNA repair ...
... germ cells in most metazoans also intentionally create double-strand breaks (DSBs) to promote DNA exchange between parental chromosomes, a process known as crossing over. Homologous recombination is employed in the repair of both genotoxic lesions and programmed DSBs and many of the core DNA repair ...
53 Gene Targeting in Human Somatic Cells
... DNA, i.e., the entire human genome (Figure 53–2, iv), that are required to localize the incoming DNA to its specific, cognate chromosomal counterpart.40 In humans, there are at least seven Rad51 family members and almost all of them have been implicated in some aspect of HR and also in human disease ...
... DNA, i.e., the entire human genome (Figure 53–2, iv), that are required to localize the incoming DNA to its specific, cognate chromosomal counterpart.40 In humans, there are at least seven Rad51 family members and almost all of them have been implicated in some aspect of HR and also in human disease ...
upstream sequence of a differentiation
... Fig. 1. Identification of rabbit K3 cDNA clones by hybrid selection. Total RNAs of a confluent culture of rabbit corneal epithelial cells were hybridized with linearized, filter-immobilized cDNA clones (B13 and B3). The hybrid-selected mRNAs were then eluted, in vitro translated in the presence of [ ...
... Fig. 1. Identification of rabbit K3 cDNA clones by hybrid selection. Total RNAs of a confluent culture of rabbit corneal epithelial cells were hybridized with linearized, filter-immobilized cDNA clones (B13 and B3). The hybrid-selected mRNAs were then eluted, in vitro translated in the presence of [ ...