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Techniques in Mouse
Techniques in Mouse

... you are interested and a second that contains gene of interest flanked by loxP sites. The gene will only be deleted where Cre is expressed. – Can also activate genes: In second line place stop signal flanked by loxP between 5’ regulatory element and gene. When stop signal is removed gene will be act ...
PDF
PDF

... The DNA inside a cell is packaged very tightly into chromosomes. Within a human cell, 23 pairs of chromosomes fit in a structure that is one-tenth the width of a human hair, but if you unwound the chromosomes, the DNA would be six feet long. All living things contain DNA recipes and use them to make ...
U(x) - Bioconductor
U(x) - Bioconductor

... (RNA-seq) in single cells. Exploratory studies have already led to insights into the dynamics of differentiation, cellular responses to stimulation and the stochastic nature of transcription. We are entering an era of single-cell transcriptomics that holds promise to substantially impact biology and ...
DNA -- The Double Helix
DNA -- The Double Helix

... The rungs of the ladder are pairs of 4 types of nitrogen bases. The bases are known by their coded letters A, G, T, C. These bases always bond in a certain way. Adenine will only bond to thymine. Guanine will only bond with cytosine. The bases can occur in any order along a strand of DNA. The order ...
PowerPoint 演示文稿
PowerPoint 演示文稿

...  modulating chromatin accessibility during transcription, replication, recombination and repair;  required for re-establishing chromatin structure on a local basis after transcription of a gene or after the repair of a DNA double-strand break;  act during DNA replication when the cellular hitone ...
File
File

... • All genes switched off • As the embryo grows, genes switch on as cells become specialised • In adults there is regular replacement of worn out cells. These can only develop into cells of a particular organ, so some genes must be switched on. ...
Syllabus in English, valid from Autumn 2015
Syllabus in English, valid from Autumn 2015

... Biochemistry, Cell and Molecular Biology 30 credits and additionally 15 credits in Molecular Biology/Molecular Life Sciences. (Three credits corresponds to approximately two weeks full-time studies). Swedish upper secondary school course English B/English 6 or equivalent. Course structure Examinatio ...
B2 Topic 1: The building blocks of cells Light microscope Light
B2 Topic 1: The building blocks of cells Light microscope Light

... 1. Use adult stem cells to make cloned embryos the embryonic stem cells could then be extracted from the clones without any natural embryos having to be killed 2. Turn specialised body cells into stem cells by reprogramming them – if this works, it will help to completely avoid the ethical problem o ...
college-prep biology midterm review
college-prep biology midterm review

... The number of chromosomes in each of your somatic cells and gametes; the number of genes in each somatic cell; definition of the word, “gene” The number of autosomes and sex chromosomes in each of your somatic cells and gametes What a karyotype, homologous chromosomes, chorionic villus sampling, and ...
The Prize for the Best Pluripotent Stem Cell Goes To………
The Prize for the Best Pluripotent Stem Cell Goes To………

... iPSCs aberrantly expressed more genes than NT ESCs as compared to IVF ESCs Overall, their findings suggest that transcription factor-mediated iPSC-generation suffers from incomplete epigenetic reprogramming, while NT-ESCs are highly similar to IVF ESC controls. Specifically, iPSCs suffer the persist ...
Cell Division and Fertilization
Cell Division and Fertilization

... Compare the DNA in the nucleus of this cell with the DNA of a cell in interphase. Do you notice how the DNA molecules are shorter and fatter (more visible). We call them chromosomes now. Did you notice that not all chromosomes are the same size? ...
Stem Cell Research
Stem Cell Research

... • Embryonic Stem Cells – Transforms into any type of cell – In a Petri dish • Umbilical Cord (“Child”) Stem Cells – From placenta / umbilical cord – “Stem Cell Bank” • Adult Stem Cells – More specialized stem cells – Difficult to locate, slower growth rate, higher rejection rate ...
CP Biology
CP Biology

... d. all of these ______ 6) If the chromatid labeled C has a gene sequence that codes for normal hemoglobin, which of the following chromatids will USUALLY have the exact same gene sequence? a. A b. B c. D d. all of these 7. Is the homologous pair of chromosomes above in a dividing or non-dividing cel ...
Viruses and Bacteria
Viruses and Bacteria

...  The trp operon is a repressible operon  An inducible operon is one that is usually off; a molecule called an inducer inactivates the repressor and turns on transcription  The classic example of an inducible operon is the lac operon, which contains genes coding for enzymes in hydrolysis and metab ...
Fall Semester Exam Review PDF
Fall Semester Exam Review PDF

... 16. What is active transport? What type of energy is used for active transport? 17. What is passive transport? What type of energy is used for passive transport? 18. When a substance is being moved into a cell, against the concentration gradient, such as A in the image below is this active or passiv ...
Life Science vocabulary
Life Science vocabulary

... clone An offspring produced by asexual reproduction that is genetically identifcal, either naturally or through artificial processes. egg cell A cell produced by a female that contains half of the number of chromosomes present in other body cells. A female reproductive cell. fertilization The union ...
Exam 4 Key Fa08
Exam 4 Key Fa08

... 3. A sequence of 3 nitrogenous bases in mRNA that code for a specific amino acid. (1 pt) [codon] 4. Mis-folded protein that causes degenerative brain diseases. (1 pt) [prion] 5. The progressive restriction of the developmental potential of a cell to only one specific fate. (1 pt) [determination] 6. ...
PPT File
PPT File

... • An interesting model for studying the role of environmental factors in epigenetic modifications – Epigenetically concordant at birth in most cases – Differences (DNA methylation and histone modifications) accumulate with age – Greatest in those who had lived together for the smallest amount of tim ...
Supplemetal Figures Legend
Supplemetal Figures Legend

... Figure S4. Knockdown of DNMT1 in U251 cells by using siRNA. DNMT1 siRNA or negative control (NC) siRNA (10nM) were transfected into U251 cells by using Lipofectamine® RNAiMAX at day 1, day 3 and day 5 after seeded into 24-well plates. Total RNA was isolated 48h after the last transfection. DNMT1 mR ...
Biology 2 2.6 2.8 cycle sheet
Biology 2 2.6 2.8 cycle sheet

... INTERPRET data on the effects of exercise on the human body. ...
Patterns in Development
Patterns in Development

... • Identity of the segments are determined by homeotic genes – specify the appendages that form on the individual segments ...
Big Idea #3
Big Idea #3

... is turned of f. When a repressor is absent, the gene is turned on.  Inducible operons: usually turned off and only become active when the repressor is lifted.  Repressible operons: usually turned on and only become inactivated when the repressor binds. ...
Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... In development there are many branching processes, whereby a given cell gives rise to two independent cell types. Such a process can be regarded as a developmental switch in which one daughter gains a new phenotype, or potential phenotype, whereas the other retains its original phenotype. This is a ...
B3 * student gap fill
B3 * student gap fill

... 1. Bacteria are very simple and have no n_____ or m______ – their DNA is in one loop called a plasmid 2. Plants have c_______ for photosynthesis, v_____ (middle) for support and a c___ w____ 3. Mass can be measured by an increase in height, w______ w_____ or dry m_______ 4. It is better to measu ...
nature v. nurture
nature v. nurture

... diseases. "This could lead to far-reaching revelations about how our environment breeds predispositions for lots of diseases, like diabetes, cancer and heart disease." Human cells have tens of thousands of genes inside them, each with its own job, such as producing energy or overseeing cell division ...
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Epigenetics in stem-cell differentiation

Embryonic stem cells are capable of self-renewing and differentiating to the desired fate depending on its position within the body. Stem cell homeostasis is maintained through epigenetic mechanisms that are highly dynamic in regulating the chromatin structure as well as specific gene transcription programs. Epigenetics has been used to refer to changes in gene expression, which are heritable through modifications not affecting the DNA sequence.The mammalian epigenome undergoes global remodeling during early stem cell development that requires commitment of cells to be restricted to the desired lineage. There has been multiple evidence suggesting that the maintenance of the lineage commitment of stem cells are controlled by epigenetic mechanisms such as DNA methylation, histone modifications and regulation of ATP-dependent remolding of chromatin structure. Based on the histone code hypothesis, distinct covalent histone modifications can lead to functionally distinct chromatin structures that influence the fate of the cell.This regulation of chromatin through epigenetic modifications is a molecular mechanism that will determine whether the cell will continue to differentiate into the desired fate. A research study performed by Lee et al. examined the effects of epigenetic modifications on the chromatin structure and the modulation of these epigenetic markers during stem cell differentiation through in vitro differentiation of murine embryonic stem (ES) cells.
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