Non-Protein Nitrogen(NPN) Compounds
... Low protein dietary intake Liver disease (lack of synthesis) Severe vomiting and/or diarrhea (loss) Increase protein synthesis ...
... Low protein dietary intake Liver disease (lack of synthesis) Severe vomiting and/or diarrhea (loss) Increase protein synthesis ...
Cholecystokinin and Trypsin Responses of Larval Red Drum
... our lab has begun to investigate the role of the digestive hormone cholecystokinin (CCK). While there are a number of other labs also investigating CCK and other digestive hormones such as bombesin, PPY, and gastrin; research into the roles of these hormones in fish is still in its infancy. Previous ...
... our lab has begun to investigate the role of the digestive hormone cholecystokinin (CCK). While there are a number of other labs also investigating CCK and other digestive hormones such as bombesin, PPY, and gastrin; research into the roles of these hormones in fish is still in its infancy. Previous ...
Comparative proteomic network signatures in seminal plasma of
... cocktail (Roche, Indianapolis, IN, USA) in phosphate buffer saline in order to prevent proteolysis during sample handling and again centrifuged at ~10,000×g for 30 min to get rid of any cellular debris. Protein concentration in the seminal plasma was determined using bicinchoninic acid (BCA) kit (Th ...
... cocktail (Roche, Indianapolis, IN, USA) in phosphate buffer saline in order to prevent proteolysis during sample handling and again centrifuged at ~10,000×g for 30 min to get rid of any cellular debris. Protein concentration in the seminal plasma was determined using bicinchoninic acid (BCA) kit (Th ...
Noni Juice - Healthoracle.org
... these proteins and so therefore has a wide range of biological activities. Heinicke hypothesizes that xeronine will interact with the dysfunctional protein and make it fold into its proper conformation, which inevitably results in a properly functioning protein. Therefore, whenever a problem arises ...
... these proteins and so therefore has a wide range of biological activities. Heinicke hypothesizes that xeronine will interact with the dysfunctional protein and make it fold into its proper conformation, which inevitably results in a properly functioning protein. Therefore, whenever a problem arises ...
Metabolic and physiological interdependencies in the
... subjected to a combination of differential pelleting and rate-zonal centrifugation using density gradients on board the research vessel to separate the symbionts and host components from each other (Figure 2). The composition of putative host- and symbiont-enriched fractions after centrifugation was ...
... subjected to a combination of differential pelleting and rate-zonal centrifugation using density gradients on board the research vessel to separate the symbionts and host components from each other (Figure 2). The composition of putative host- and symbiont-enriched fractions after centrifugation was ...
Localization of Low-sulfur Keratin Proteins in the Wool Follicle Using
... with the panel of monoclonal antibodies was investigated using indirect immunofluorescent. The results are listed in Table I and shown schematically in Fig. 3. Despite the disadvantage that the monoclonal antibodies all recognize more than one protein component, the studies indicate a specific patte ...
... with the panel of monoclonal antibodies was investigated using indirect immunofluorescent. The results are listed in Table I and shown schematically in Fig. 3. Despite the disadvantage that the monoclonal antibodies all recognize more than one protein component, the studies indicate a specific patte ...
MND Australia International Research Update December 2016
... systems, a cascade of special enzymes direct little proteins, called ubiquitin, to unwanted or old proteins to target them for removal. This cascade begins with an E1 enzyme that activates the ubiquitin, followed by an E2 enzyme that works with an E3 enzyme to bind ubiquitin to its target protein. T ...
... systems, a cascade of special enzymes direct little proteins, called ubiquitin, to unwanted or old proteins to target them for removal. This cascade begins with an E1 enzyme that activates the ubiquitin, followed by an E2 enzyme that works with an E3 enzyme to bind ubiquitin to its target protein. T ...
NHS ester - BroadPharm
... protonated, and no modification takes place. At pH that is higher than optimal, the quick hydrolysis of NHS ester can result in low yield of modification. Optimal pH value for the modification is 8.3-8.5. Water is the most common solvent for labeling. If NHS ester being used is poorly soluble in wat ...
... protonated, and no modification takes place. At pH that is higher than optimal, the quick hydrolysis of NHS ester can result in low yield of modification. Optimal pH value for the modification is 8.3-8.5. Water is the most common solvent for labeling. If NHS ester being used is poorly soluble in wat ...
New insight into plant intramembrane proteases
... plant and its responses to a changeable environment. Recent research has shown that proteases are not only engaged in quality control and protein turnover processes but also participate in the process which is known as regulated membrane proteolysis (RIP). Four families of integral membrane protease ...
... plant and its responses to a changeable environment. Recent research has shown that proteases are not only engaged in quality control and protein turnover processes but also participate in the process which is known as regulated membrane proteolysis (RIP). Four families of integral membrane protease ...
PowerPoint 演示文稿
... transcipts and (2) many mRNAs for hormones in eukaryotes (e.g. pro-opiomelanocortin) are translated to give a single polypeptide chain that is cleaved subsequently by specific proteases to produce multiple mature protein from one translation product. The parent polypeptide is called polyprotein. ...
... transcipts and (2) many mRNAs for hormones in eukaryotes (e.g. pro-opiomelanocortin) are translated to give a single polypeptide chain that is cleaved subsequently by specific proteases to produce multiple mature protein from one translation product. The parent polypeptide is called polyprotein. ...
Proteins and their Ligands: Their Importance and how to Crystallize
... The importance of structural biology has been highlighted in the past few years not only as part of drug discovery programs in the pharmaceutical industry but also by structural genomics programs. Although the function of a protein can be studied by several biochemical and or biophysical techniques ...
... The importance of structural biology has been highlighted in the past few years not only as part of drug discovery programs in the pharmaceutical industry but also by structural genomics programs. Although the function of a protein can be studied by several biochemical and or biophysical techniques ...
Sequence-based predictions of membrane-protein topology, homology and insertion
... taken place, each of which is required for the development, survival and propagation of the cell and the genetic material it carries. Key players in this chemical factory of life are the proteins, catalyzing the specific conversion of substrates to products, regulating each others' concentrations, t ...
... taken place, each of which is required for the development, survival and propagation of the cell and the genetic material it carries. Key players in this chemical factory of life are the proteins, catalyzing the specific conversion of substrates to products, regulating each others' concentrations, t ...
Article
... Beans were called "poor man's meat" because until the mid-twentieth century, when meat was still a scarce and precious commodity, legumes were the main source of protein, especially for people who, living far from the sea, could not take advantage of fishing. The proteins of legumes are not as compl ...
... Beans were called "poor man's meat" because until the mid-twentieth century, when meat was still a scarce and precious commodity, legumes were the main source of protein, especially for people who, living far from the sea, could not take advantage of fishing. The proteins of legumes are not as compl ...
Determination of protein regions responsible for interactions of
... [11]. CD63 is a member of the tetraspanin family, in which most members are cell-surface proteins that are characterized by the presence of four transmembrane segments [12]. The tetraspanin proteins mediate signal transduction events that play roles in the regulation of cell development, activation, ...
... [11]. CD63 is a member of the tetraspanin family, in which most members are cell-surface proteins that are characterized by the presence of four transmembrane segments [12]. The tetraspanin proteins mediate signal transduction events that play roles in the regulation of cell development, activation, ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
... 33 kDa protein was cleaved at 16Y by chymotrypsin and at 18E by V8 protease (Eaton-Rye and Murata 1989), while the cyanobacterial 33 kDa protein was cleaved primarily at 156F and 190F by chymotrypsin (Motoki et al. 1998). Another line of evidence suggesting a possible difference in the structure of ...
... 33 kDa protein was cleaved at 16Y by chymotrypsin and at 18E by V8 protease (Eaton-Rye and Murata 1989), while the cyanobacterial 33 kDa protein was cleaved primarily at 156F and 190F by chymotrypsin (Motoki et al. 1998). Another line of evidence suggesting a possible difference in the structure of ...
Document
... • Used with cellular component terms • To describe proteins that are transiently or peripherally associated with an organelle or complex ...
... • Used with cellular component terms • To describe proteins that are transiently or peripherally associated with an organelle or complex ...
The potato tuber mitochondrial proteome
... likely that an in-depth proteomic study of one type of plant mitochondria would identify ...
... likely that an in-depth proteomic study of one type of plant mitochondria would identify ...
(PSD) November 2015 PBAC Meeting
... In consideration of the submission, the Nutritional Products Working Party (NPWP) noted: This product has a similar proportion of amino acids to the appropriate comparator, PKU Cooler 15®; but it does not contain choline. There is an RDI level for choline, and patients on a PKU-suitable diet are l ...
... In consideration of the submission, the Nutritional Products Working Party (NPWP) noted: This product has a similar proportion of amino acids to the appropriate comparator, PKU Cooler 15®; but it does not contain choline. There is an RDI level for choline, and patients on a PKU-suitable diet are l ...
ANALYSIS OF A BACTERIAL SERINE/THREONINE KINASE
... noted for its regulation of dsbA expression in this organism. The crystal structure of RdoA’s homologue, YihE from Escherichia coli, revealed a basic bi-lobal kinase domain that is a hallmark of the eukaryotic Ser/Thr, Tyr protein kinase superfamily. YihE however, bears the greatest structural simil ...
... noted for its regulation of dsbA expression in this organism. The crystal structure of RdoA’s homologue, YihE from Escherichia coli, revealed a basic bi-lobal kinase domain that is a hallmark of the eukaryotic Ser/Thr, Tyr protein kinase superfamily. YihE however, bears the greatest structural simil ...
Partial Purification and Characterization of the Maize Mitochondrial
... immunodetection of proteins bound to nitrocellulose membranes were performed as previously described (Luethy et al., 1995a). E1a monoclonal antibodies were raised against maize protein (Luethy et al., 1995a). E1b polyclonal antibodies were raised against recombinant Arabidopsis thaliana protein (M. ...
... immunodetection of proteins bound to nitrocellulose membranes were performed as previously described (Luethy et al., 1995a). E1a monoclonal antibodies were raised against maize protein (Luethy et al., 1995a). E1b polyclonal antibodies were raised against recombinant Arabidopsis thaliana protein (M. ...
Co-translational Folding
... The mechanism by which it happens The short time span in which it happens – How an amino acid sequence folds into unique 3-D shape? – How can native conformation be found and recognized? – The entire duration of the folding process varies dramatically depending on the protein of interest – Slowest f ...
... The mechanism by which it happens The short time span in which it happens – How an amino acid sequence folds into unique 3-D shape? – How can native conformation be found and recognized? – The entire duration of the folding process varies dramatically depending on the protein of interest – Slowest f ...
Product_Cheluminate_100915
... The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline condition followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. In the second step, ...
... The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline condition followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. In the second step, ...
Data-driven docking for the study of biomolecular complexes
... Utrecht University, 3584CH, Utrecht, The Netherlands. Email: [email protected] With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their ofte ...
... Utrecht University, 3584CH, Utrecht, The Netherlands. Email: [email protected] With the presently available amount of genetic information, a lot of attention focuses on systems biology and in particular on biomolecular interactions. Considering the huge number of such interactions, and their ofte ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.