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Leeds Protein Production Facility Tris buffered saline Tris buffered saline (TBS) is used for any number of buffering/washing situations, most notably during western blots. TBS is made up as follows: o 50 mM Tris o 150 mM NaCl o Adjust pH to 7.5 with HCl TBS can also have Tween 20 added to it to make TBST, which is used in the washing of membranes for western blots To make TBST: o Add 1:1000 v/v Tween 20 to TBS o E.g. add 1 ml Tween 20 to 1 l TBS Last updated July 2016 Dr Brian Jackson, PPF Manager