Isolation of Vibrio harveyi Acyl Carrier Protein and the fabG, acpP
... thetase (29). Final purification was 1,700-fold, with a typical yield of 60 mg of ACP per kilogram (wet weight) of V. harveyi cells. Purified V. harveyi ACP migrated as a single 20-kDa band on a sodium dodecyl sulfate (SDS)-polyacrylamide gel, similar to E. coli ACP (Mr, 8,847), which is known to ex ...
... thetase (29). Final purification was 1,700-fold, with a typical yield of 60 mg of ACP per kilogram (wet weight) of V. harveyi cells. Purified V. harveyi ACP migrated as a single 20-kDa band on a sodium dodecyl sulfate (SDS)-polyacrylamide gel, similar to E. coli ACP (Mr, 8,847), which is known to ex ...
Slide 1
... hnRNP and SR proteins... Multimerization of hnRNP A1 from a high affinity ESS can be sterically blocked by interaction of SF2/ASF with ESE”. Show predicted results of gel mobility shift experiments. ...
... hnRNP and SR proteins... Multimerization of hnRNP A1 from a high affinity ESS can be sterically blocked by interaction of SF2/ASF with ESE”. Show predicted results of gel mobility shift experiments. ...
Bovine Peptidoglycan Recognition Protein
... bPGRP-S for 60 min at 37°C with continuous agitation. Candida albicans 16820, Cryptococcus neoformans 271a, S. aureus 502a, and Escherichia coli ML35 were suspended in 10 mM PIPES and 5 mM glucose (pH 7.4). Similar incubations were conducted with S. typhimurium 10428 PhoP⫺ and Listeria monocytogenes ...
... bPGRP-S for 60 min at 37°C with continuous agitation. Candida albicans 16820, Cryptococcus neoformans 271a, S. aureus 502a, and Escherichia coli ML35 were suspended in 10 mM PIPES and 5 mM glucose (pH 7.4). Similar incubations were conducted with S. typhimurium 10428 PhoP⫺ and Listeria monocytogenes ...
THE THREE-DIMENSIONAL STRUCTURE OF PROTEINS
... Chapter 2, this entropy term is the major thermodynamic driving force for the association of hydrophobic groups in aqueous solution. Hydrophobic amino acid side chains therefore tend to be clustered in a protein’s interior, away from water. Under physiological conditions, the formation of hydrogen b ...
... Chapter 2, this entropy term is the major thermodynamic driving force for the association of hydrophobic groups in aqueous solution. Hydrophobic amino acid side chains therefore tend to be clustered in a protein’s interior, away from water. Under physiological conditions, the formation of hydrogen b ...
Protein splicing of PRP8 mini-inteins from species of the genus
... GST-Intein-His-tag (GIH) precursor to the 32.2-kDa GHspliced product and the 17.7–18.8-kDa excised intein (I). Cleavage at the N-terminal splice junction would be expected to result in the production of G (29.3 kDa) and IH (20.6 to 21.7 kDa). SDS-PAGE analysis resulted in bands consistent with the f ...
... GST-Intein-His-tag (GIH) precursor to the 32.2-kDa GHspliced product and the 17.7–18.8-kDa excised intein (I). Cleavage at the N-terminal splice junction would be expected to result in the production of G (29.3 kDa) and IH (20.6 to 21.7 kDa). SDS-PAGE analysis resulted in bands consistent with the f ...
C7orf30 is necessary for biogenesis of the large
... S. cerevisiae Atp25p (Genbank Accession No. NP_013816) and human C7orf30 (Genbank Accession No. NP_612455) were extracted from the PFAM database (42) and aligned using CustalW2. The boundaries of the sequence segment used in the alignment are indicated. Colons and dots denote chemical similarity bet ...
... S. cerevisiae Atp25p (Genbank Accession No. NP_013816) and human C7orf30 (Genbank Accession No. NP_612455) were extracted from the PFAM database (42) and aligned using CustalW2. The boundaries of the sequence segment used in the alignment are indicated. Colons and dots denote chemical similarity bet ...
Protein sequence analysis
... penalised. If you don't like long gaps, just increase the extension gap penalty. Usually you will expect a few long gaps rather than many short gaps, so the gap extension penalty should be lower than the gap penalty. An exception is where one or both sequences are single reads with possible sequenci ...
... penalised. If you don't like long gaps, just increase the extension gap penalty. Usually you will expect a few long gaps rather than many short gaps, so the gap extension penalty should be lower than the gap penalty. An exception is where one or both sequences are single reads with possible sequenci ...
PolyWAX LP ERLIC instructions
... B in no less than 20’ if the sample is likely to contain peptides with at least 3 phosphate groups. For analysis of tryptic digests containing large numbers of phosphate groups, running a longer gradient and collecting more fractions will result in significantly more phosphopeptides being identified ...
... B in no less than 20’ if the sample is likely to contain peptides with at least 3 phosphate groups. For analysis of tryptic digests containing large numbers of phosphate groups, running a longer gradient and collecting more fractions will result in significantly more phosphopeptides being identified ...
Function and biotechnology of extremophilic enzymes in low water
... Enzymes from extremophilic microorganisms usually catalyze chemical reactions in non-standard conditions. Such conditions promote aggregation, precipitation, and denaturation, reducing the activity of most non-extremophilic enzymes, frequently due to the absence of sufficient hydration. Some extremo ...
... Enzymes from extremophilic microorganisms usually catalyze chemical reactions in non-standard conditions. Such conditions promote aggregation, precipitation, and denaturation, reducing the activity of most non-extremophilic enzymes, frequently due to the absence of sufficient hydration. Some extremo ...
No Slide Title
... gp40 and gp15, are products of proteolytic cleavage of a 49 kDa precursor protein expressed in intracellular stages. The Cpgp40/15 locus is highly polymorphic ...
... gp40 and gp15, are products of proteolytic cleavage of a 49 kDa precursor protein expressed in intracellular stages. The Cpgp40/15 locus is highly polymorphic ...
H +
... ions down either a concentration gradient, in the case of water, or an electric potential gradient, in the case of certain ions from an area of higher concentration to lower concentration. While water molecules can directly cross the membrane by simple diffusion, as mentioned above, their transport ...
... ions down either a concentration gradient, in the case of water, or an electric potential gradient, in the case of certain ions from an area of higher concentration to lower concentration. While water molecules can directly cross the membrane by simple diffusion, as mentioned above, their transport ...
Title Optimization of Amino Acid Parameters for Correspondence of
... from the Ca coordinatesby picking up C„, successivelygreater than 0.6 for more than ten residues. Then these selected segments shown in Table IV are superposed with. each other, so that all these corresponding segments have the good structure correspondences with r.m.s. deviation, 1.42A, on average. ...
... from the Ca coordinatesby picking up C„, successivelygreater than 0.6 for more than ten residues. Then these selected segments shown in Table IV are superposed with. each other, so that all these corresponding segments have the good structure correspondences with r.m.s. deviation, 1.42A, on average. ...
Template-Synthesized Protein Nanotubes
... after deposition of 6-layer GOD nanotubes within the pores. Evidence for nanotube deposition can be seen by the decrease in the average pore diameter after deposition. An approximate measure of the wall thickness of the nanotubes can be obtained from such images; wall thickness of ∼15 nm and ∼30 nm ...
... after deposition of 6-layer GOD nanotubes within the pores. Evidence for nanotube deposition can be seen by the decrease in the average pore diameter after deposition. An approximate measure of the wall thickness of the nanotubes can be obtained from such images; wall thickness of ∼15 nm and ∼30 nm ...
the elastin gene
... partially solubilise the protein? How does increased crosslinking affect keratin's properties? 7. Keratin is the main component of hair. How are individual keratin molecules organised in a hair fibre? 8. Changes in keratin structure underlie temporary and permanent waving of hair. How? 9. If your ha ...
... partially solubilise the protein? How does increased crosslinking affect keratin's properties? 7. Keratin is the main component of hair. How are individual keratin molecules organised in a hair fibre? 8. Changes in keratin structure underlie temporary and permanent waving of hair. How? 9. If your ha ...
HEALTH BENEFITS OF PULSES
... draining the pulses every 4 to 8 hours and this process usually takes 1 to 5 days. Sprouting pulses is vital to render them edible in a raw state and make them more easily digestible. Interestingly, sprouted pulses can significantly diminish polyphenols and tannins, and the protein, carbohydrates an ...
... draining the pulses every 4 to 8 hours and this process usually takes 1 to 5 days. Sprouting pulses is vital to render them edible in a raw state and make them more easily digestible. Interestingly, sprouted pulses can significantly diminish polyphenols and tannins, and the protein, carbohydrates an ...
a i5388e
... Incredibly rich in their nutritional value, pulses are small but densely packed with proteins – double that found in wheat and three times that of rice. Unlike animal food sources of protein such as beef or milk, pulses do not contain residues of hormones or antibiotics used in animal production, bu ...
... Incredibly rich in their nutritional value, pulses are small but densely packed with proteins – double that found in wheat and three times that of rice. Unlike animal food sources of protein such as beef or milk, pulses do not contain residues of hormones or antibiotics used in animal production, bu ...
Slides
... We have a large number of predominantly apo form structures being determined by structural genomics and functionally driven structure determination ...
... We have a large number of predominantly apo form structures being determined by structural genomics and functionally driven structure determination ...
Responses of Growing Broilers to Diets with Increased Sulfur Amino
... similar to that of a commercial operation with normal environmental conditions for temperature and ventilation, but having 24 h of light per day. Corn, soybean meal, and poultry by-product meal (viscera and feet plus head) were obtained from a commercial integrator in the amount needed to compose th ...
... similar to that of a commercial operation with normal environmental conditions for temperature and ventilation, but having 24 h of light per day. Corn, soybean meal, and poultry by-product meal (viscera and feet plus head) were obtained from a commercial integrator in the amount needed to compose th ...
Protein structure
... The extent of the improvements in the Xray crystallographic method have meant that the rate-limiting steps in structure determination are now the ability to grow well-diffracting crystals, and therefore, the availability of crystallizable samples. The considerable difficulties associated with the ne ...
... The extent of the improvements in the Xray crystallographic method have meant that the rate-limiting steps in structure determination are now the ability to grow well-diffracting crystals, and therefore, the availability of crystallizable samples. The considerable difficulties associated with the ne ...
Hemoglobin
... deprivation oxymyoglobin releases its bound oxygen which is then used for metabolic purposes. The tertiary structure of myoglobin is that of a typical water soluble globular protein. Its secondary structure is unusual in that it contains a very high proportion (75%) of α-helical secondary structure. ...
... deprivation oxymyoglobin releases its bound oxygen which is then used for metabolic purposes. The tertiary structure of myoglobin is that of a typical water soluble globular protein. Its secondary structure is unusual in that it contains a very high proportion (75%) of α-helical secondary structure. ...
ARTÍCULOS
... genes (pET plasmids) were introduced into BL21(DE3)(pLysE) by transformation. One-liter cultures were grown at 30°C to an optical density at 600 nm of 1.0. At this point, IPTG (isopropyl--D-galactopyranoside) was added to 0.5 mM, and incubation was carried out for 15 min. Cells were then pelleted b ...
... genes (pET plasmids) were introduced into BL21(DE3)(pLysE) by transformation. One-liter cultures were grown at 30°C to an optical density at 600 nm of 1.0. At this point, IPTG (isopropyl--D-galactopyranoside) was added to 0.5 mM, and incubation was carried out for 15 min. Cells were then pelleted b ...
9c63$$mr30 Black separation
... was separated by ultracentrifugation at 150,000g for 2 hours. This soluble cell fraction was used for the isolation of cystalysin by solid (70% to 90%) (NH4)2SO4 (AS) precipitation. DEAE-sephacel chromatography. Approximately 15 mL of a 70% – 90% AS fraction was applied to a DEAE-sephacel column (2. ...
... was separated by ultracentrifugation at 150,000g for 2 hours. This soluble cell fraction was used for the isolation of cystalysin by solid (70% to 90%) (NH4)2SO4 (AS) precipitation. DEAE-sephacel chromatography. Approximately 15 mL of a 70% – 90% AS fraction was applied to a DEAE-sephacel column (2. ...
mTORC kinase assays
... wash buffer 3 times. For mTORC1 kinase assay, add a wash with CHAPS wash buffer with 500mM NaCl to remove PRAS40. 5. The immunoprecipitates are washed once in kinase wash buffer. Resuspend the immunoprecipitates in kinase wash buffer and make 10-12 aliquots (~2.5 ul beads per reaction). Spin, and re ...
... wash buffer 3 times. For mTORC1 kinase assay, add a wash with CHAPS wash buffer with 500mM NaCl to remove PRAS40. 5. The immunoprecipitates are washed once in kinase wash buffer. Resuspend the immunoprecipitates in kinase wash buffer and make 10-12 aliquots (~2.5 ul beads per reaction). Spin, and re ...
Mitochondrial protein acetylation regulates metabolism
... whom correspondence should be addressed (email matthew.hirschey@duke. edu). ...
... whom correspondence should be addressed (email matthew.hirschey@duke. edu). ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.