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Nature Medicine Research Highlights: New from NPG
Nature Medicine Research Highlights: New from NPG

... the authors showed that in the absence of telomerase the time to tumor emergence is increased, whereas reactivating telomerase in previously telomerase-deficient cells shortens the tumor latency and results in more aggressive malignancy. Hu et al. found that turning off telomerase in lymphoma cells ...
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DNA - Community College of Rhode Island
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... ▪ The structure of DNA is a double helix or "twisted ladder" structure. The sides are composed of alternating phosphate-sugar groups and "rungs of the DNA ladder" are composed of complementary nitrogenous base pairs (always adenine, A, to thymine, T, and cytosine, C, to guanine, G) joined by weak hy ...
CH 14 Gene Expression: From Gene to Protein and
CH 14 Gene Expression: From Gene to Protein and

... that lacks a follicle – there is no _________________ DNA, but there is mitochondrial DNA in it!!!! Extra Info / FYI: Reading the DNA code to locate genes can become even more complicated than you think. For Example, the DNA code for my Bacteriophage Virus (Pipefish) had to be read to ID the genes. ...
The Operon - dl.edi
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... Absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. The activity of RNA polymerase also depends on the presence of another DNAbinding protein called catabolite activator protein or CAP. Like the lac repressor, CAP has two types of binding sites ...
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... and subjected to gPCR using specific primers for both CA2 and CAL2 or CAL1 genes and T-DNA border primers (LB). Double homozygous plants were identified for ca2cal2 and ca2cal1 double mutants. (b) Transcript levels of WT and double mutant ca2cal1 plants were assessed by RT-PCR at 40 cycles using spe ...
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... anaerobic process during which carbohydrates are broken down for energy production. We can detect whether a specific carbohydrate is fermented by looking for common end products of fermentation. Acid end products and/or acid and gas end products will distinguish these bacteria: Bacillus subtilis, Es ...
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Lecture 7 Manipulation of gene expression and secretion of foreign

... the translational level. The most active construct contained seven enhancer elements and directed much higher level of foreign gene expression in both transgenic tobacco and rice plants than when the 355 promoter alone was used. These promoter constructs directed a wide range of foreign gene express ...
DNA, Proteins, and Biotechnology
DNA, Proteins, and Biotechnology

... Outline the use of polymerase chain reaction (PCR) to copy and amplify minute quantities of DNA. State that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size. State that gel electrophoresis of DNA is used in DNA profiling. Describe the appl ...
Regulating Evolution - Nicolas Gompel`s lab
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... The expression of a gene entails the transcription of the DNA sequence into a messenger RNA (mRNA) version and the translation of that mRNA into a protein sequence. The expression of most genes is regulated at the transcriptional level— cells do not waste energy making ­mRNAs and proteins they do no ...
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... proteins, the rest includes RNA coding, promoter, terminators etc. In contrast, the Human genome: 3,000,000,000 base pairs and about 25,000 genes. Only 2% of the Human genome codes for proteins. The rest is……RNA regulatory network? Human genes are also segmented into Exon and Introns, with alternati ...
unit 7 exam study guide
unit 7 exam study guide

... 20. If the sequence of nucleotides on the original DNA strand was A – G – G – C – T – A, what would be the nucleotide sequence on the complementary strand of DNA? 21. Define DNA replication. 22. Why must DNA be replicated? 23. Explain the term “semi-conservative” and how it applies to replication. 2 ...
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Protein Sequencing

... Further, the accuracy of each cycle is 98%. So after 60 steps the accuracy is less than 30%. Thus, this method cannot be used for sequencing of proteins larger than 50 amino acids. In case of larger proteins it has to be broken down to short peptide fragments using cleavage proteases such as trypsin ...
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Final Exam answer key

... a. (4 pts) Which enzymes would you use to cut the fragment and the vector to be sure the gene was cloned into the vector so that the protein could be expressed from the promoter on the vector. Explain your choice. BamH1 and HindIII will cut the insert and the vector in the correct places. Because th ...
- Cal State LA - Instructional Web Server
- Cal State LA - Instructional Web Server

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Point mutation



A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.
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