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- Iranian Journal of Analytical Chemistry
- Iranian Journal of Analytical Chemistry

... and pharmaceutical compounds and also can be applied in synthesize of oligopeptides or other larger size biomolecules. They have also found applications in such areas as the biodegradable plastics industry [3], drug delivery systems [4] or in stereoselective laboratory synthesis [5]. Amino acids con ...
Purification and Characterization of
Purification and Characterization of

The Synthesis of Enzymes Concerned in Bacterio
The Synthesis of Enzymes Concerned in Bacterio

NTPase/helicase of Flaviviridae: inhibitors and inhibition of the
NTPase/helicase of Flaviviridae: inhibitors and inhibition of the

... et al., 1996) have shown that removal of the NH2-terminal 148 amino acids enhanced the NTPase activity of JEV NTPase/helicase. On the other hand, a comparative study with recombinant full-length NS3 and its isolated NH2- and COOH-terminal domains, performed by Gallinari et al. (1998), did not reveal ...
Amino acid and soluble nitrogen evolution throughout ripening of
Amino acid and soluble nitrogen evolution throughout ripening of

... when studying the quality of Kefalograviera cheese produced from raw and refrigerated ewe’s milk, concluded that cold storage accelerated flavor development and enhanced flavor of the low-fat variety. Witting (1981) reported increased coagulation time for refrigerated milk, thus producing a less firm c ...
THE INFLUENCE OF NUTRITIONAL PHOSPHATE DEPRIVATION ARABIDOPSIS THALIANA
THE INFLUENCE OF NUTRITIONAL PHOSPHATE DEPRIVATION ARABIDOPSIS THALIANA

... pathogen responses. The relationship between mRNA levels and relative amounts of selected secretome proteins was assessed. The results indicate that transcriptional control is but one of many factors contributing to Arabidopsis Pi starvation responses and highlight the importance of parallel biochem ...
Bio 226: Cell and Molecular Biology
Bio 226: Cell and Molecular Biology

... Assimilating N and S is very expensive! • Reducing NO3- to NH4+ costs 8 e- (1 NADPH + 6 Fd) • Assimilating NH4+ into amino acids also costs ATP + e• Nitrogen fixation costs 16 ATP + 8 e• SO42- reduction to S2- costs 8 e- + 2ATP • S2- assimilation into Cysteine costs 2 more e• Most explosives are bas ...
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... even-chain fatty acids and ketone body not replenish ...
Altered pathogenicity, immunity, tissue tropism and 3` genome
Altered pathogenicity, immunity, tissue tropism and 3` genome

... Coronaviruses belong to the family Coronaviridae and the order Nidovirales, and are classified into three groups based on the absence of genetic and antigenic relationships between the species of the different groups [1, 2]. They are known to cause upper and lower respiratory diseases, gastroenterit ...
BIOSYNTHESIS OF AMINO ACIDS, NUCLEOTIDES, AND
BIOSYNTHESIS OF AMINO ACIDS, NUCLEOTIDES, AND

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Acetaminophen and Salicylates Toxicity and Management
Acetaminophen and Salicylates Toxicity and Management

Cardiac mitochondrial matrix and respiratory complex - AJP
Cardiac mitochondrial matrix and respiratory complex - AJP

... assumed that when movement is required at subunit interfaces during the catalytic cycle, one or more phosphorylation events could potentially block activity. Phosphorylation sites close to the entry or exit points for protons in respiratory enzymes are also potentially relevant if they can block acc ...
Chylomicron Remnants and Nonesterified Fatty Involved in Lipogenesis in Rats
Chylomicron Remnants and Nonesterified Fatty Involved in Lipogenesis in Rats

... indicated in the figure legends. Total RNA was isolated using Tri-Reagent (Ambion) according to the manufacturer’s instructions. RNA (150 ng) was DNase I-treated and the amount of mRNA for G6PD, SREBP-1c, fatty acid synthase (FAS),6 acetyl-CoA carboxylase-1 (ACC-1), ATPcitrate lyase (ATP-CL), stearo ...
Cholecystokinin in White Sea Bream: Molecular Cloning, Regional
Cholecystokinin in White Sea Bream: Molecular Cloning, Regional

... gastro-intestinal tract (GIT), as well as the central and peripheral nervous system in both mammalian and non-mammalian species. In the digestive organs, CCK is synthesized by specific endocrine cells scattered in the intestinal mucosa, as a prepro-CCK polypeptide that is enzymatically cleaved post- ...
Analysis of the bipartite networks of domain compositions and
Analysis of the bipartite networks of domain compositions and

... the substrates and enzymes of reactions and the metabolic pathways they belong to. Components of the same enzyme complex are treated as co-occurring proteins in the same reaction, while distinct proteins catalyzing the same reaction are treated as alternative enzymes. 1661 E. coli reactions and 1313 ...
A genetically encoded fluorescent tRNA is active in live
A genetically encoded fluorescent tRNA is active in live

... live cells. The principles of developing this tRNA fusion are accessible to all cells amendable to genetic engineering. In this work, we made tRNA fusion with a Spinach aptamer. Recent in vitro selections have isolated RNA aptamers that bind to a range of synthetic GFP-like fluorophores, generating ...
Lactobacilli carry cryptic genes encoding peptidase
Lactobacilli carry cryptic genes encoding peptidase

... analysis. With reverse transcription-PCR, however, the presence of monocistronic orfZ transcripts was established. The orfZ gene could also be overexpressed in E. coli using the vector pKK223-3. The size of t h e protein synthesized, 41 kDa, confirmed the molecular mass of OrfZ calculated according ...
Biochi~ic~a - ScienceDirect
Biochi~ic~a - ScienceDirect

... Isozymic forms of glycogen phosphorylase Glycogen phosphorylase has three isozymic forms: these are known as the muscle, liver and brain isozymes. The muscle form has been assigned to the long arm of chromosome 11 by high resolution chromosome sorting and DNA spot-blot analysis [4]. The liver form i ...
Identification of a Cis-Acting Element of ART1, a
Identification of a Cis-Acting Element of ART1, a

... both internally and externally. Internal detoxification is achieved by sequestration of Al into the vacuoles and chelation with organic acids including citrate and oxalate, which is seen in some Al-accumulating plants such as hydrangea (Hydrangea macrophylla) and buckwheat (Fagopyrum esculentum; Ma, ...
FORMATTED - revised ENZYMology
FORMATTED - revised ENZYMology

... Kuhne ( 1878) used the term enzyme to distinguish this unorganized ferments. In Greek enzyme means ‘in yeast’. Concrete evidence for this assumption was provided by Buchner in 1897. He showed the production of alcohols from sugars using cell free extract of yeast. Emil Fischer (1894) showed the spec ...
STARCH AND OTHER POLYSACCHARIDES
STARCH AND OTHER POLYSACCHARIDES

... Starch synthase (ADP-1-Glc-1-4-α-glucan-4-D-glucosyltransferase) occurs, like the above one, in many isoforms of various localisation. Different isoform structures result from corresponding gene modifications, which controlled their synthesis during evolution; this depends on plant family or even sp ...
Answers to Quiz Questions
Answers to Quiz Questions

... All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, 999 Riverview Drive, Suite 208, Totowa, NJ 07512 USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with ...
Three-dimensional Structure of Goose
Three-dimensional Structure of Goose

... activity six to seven times greater than hen egg-white lysozyme. Black swan goose-type lysozyme consists of 185 amino acid residues (Mr = 20 400) and its amino acid sequence (Simpson et al. 1980) shows no homology with either hen egg-white or phage T4lysozymes, but closely resembles the Embden goose ...
The Role of Different Sugars, Amino Acids and Few Other
The Role of Different Sugars, Amino Acids and Few Other

... acids requirement of different H. pylori strains, but in general, most of the strains have an absolute requirement for 7-8 amino acids including arginine, histidine, isoleucine, leucine, methionine, phenylalanine and all of the strains require valine. Some amino acids such as tyrosine, cysteine and ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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