HEMAGGLUTINATION BY PURIFIED TYPE I ESCHERICHIA
... made in PBS-BSA so t h a t 25 pl r e m a i n e d in each well. To each of these wells was t h e n added 25 pl of a 0.25% concentration of the test erythrocytes, the trays were sealed with acetate tape, agitated for 30 s and incubated at 24°C for 2-3 h, at which time end points were recorded. If end ...
... made in PBS-BSA so t h a t 25 pl r e m a i n e d in each well. To each of these wells was t h e n added 25 pl of a 0.25% concentration of the test erythrocytes, the trays were sealed with acetate tape, agitated for 30 s and incubated at 24°C for 2-3 h, at which time end points were recorded. If end ...
Caspaar Bijleveld and Math JH Geelen
... [lO,ll]. These difficulties can be circumvented by coupling the carboxylation reaction with the fatty acid synthase reaction. In this coupled system the rate of formation of labelled malonyl-CoA from radioactive acetyl-CoA is measured by determining the incorporation of 14C into long-chain fatty aci ...
... [lO,ll]. These difficulties can be circumvented by coupling the carboxylation reaction with the fatty acid synthase reaction. In this coupled system the rate of formation of labelled malonyl-CoA from radioactive acetyl-CoA is measured by determining the incorporation of 14C into long-chain fatty aci ...
Malate Dehydrogenases – Structure and Function
... Primary and secondary structure Malate dehydrogenases belong to the NAD-dependent dehydrogenases, which are one of the largest and best-studied families of nucleotide-binding proteins: over 100 different members have already been identified. In general, dehydrogenases are large protein molecules, e. ...
... Primary and secondary structure Malate dehydrogenases belong to the NAD-dependent dehydrogenases, which are one of the largest and best-studied families of nucleotide-binding proteins: over 100 different members have already been identified. In general, dehydrogenases are large protein molecules, e. ...
Fatty Acid Oxid
... Free fatty acids, which in solution have detergent properties, are transported in the blood bound to albumin, a serum protein produced by the liver. Several proteins have been identified that facilitate transport of long chain fatty acids into cells, including the plasma membrane protein CD36. ...
... Free fatty acids, which in solution have detergent properties, are transported in the blood bound to albumin, a serum protein produced by the liver. Several proteins have been identified that facilitate transport of long chain fatty acids into cells, including the plasma membrane protein CD36. ...
Communication
... damage in vivo, we developed a quantitative assay for measuring levels of 3-nitrotyrosine. The method combines gas chromatography with stable isotope dilution mass spectrometry (GC-MS). Using this assay, we first investigated the relative yields of protein-bound oxidation products in bovine serum al ...
... damage in vivo, we developed a quantitative assay for measuring levels of 3-nitrotyrosine. The method combines gas chromatography with stable isotope dilution mass spectrometry (GC-MS). Using this assay, we first investigated the relative yields of protein-bound oxidation products in bovine serum al ...
Methionhe synthesis in Proteus mirabilis
... Professor Fredericq, Likge) the more selective method of Gorini & Kaufnian (1960) was used. The nutritional requirements of mutants were determined auxanographically (Lederberg, 1946); sulphur sources were applied in drops containing 3 mg. S/ml. The abbreviations metF, cysE, hserlmet, etc., are used ...
... Professor Fredericq, Likge) the more selective method of Gorini & Kaufnian (1960) was used. The nutritional requirements of mutants were determined auxanographically (Lederberg, 1946); sulphur sources were applied in drops containing 3 mg. S/ml. The abbreviations metF, cysE, hserlmet, etc., are used ...
RNA–Amino Acid Binding - University of Colorado-MCDB
... multiple directional bonds to RNA. Recurrence of interactions between a-carbon substituents and an array of Gcontaining base pairs in the lysine riboswitch confirms that such interactions are probable, independent of mooring of the amino acid to the RNA site via an adenosine residue, as occurs in SA ...
... multiple directional bonds to RNA. Recurrence of interactions between a-carbon substituents and an array of Gcontaining base pairs in the lysine riboswitch confirms that such interactions are probable, independent of mooring of the amino acid to the RNA site via an adenosine residue, as occurs in SA ...
Amino Acid Sequences and Evolutionary Relationships - Parkway C-2
... How do similarities in amino acid sequences of various species provide evidence for evolution? An important technique used in determining evolutionary relationships is the biochemical comparison of organisms. Organisms that are not similar in physical appearance may show similarity in amino acid seq ...
... How do similarities in amino acid sequences of various species provide evidence for evolution? An important technique used in determining evolutionary relationships is the biochemical comparison of organisms. Organisms that are not similar in physical appearance may show similarity in amino acid seq ...
Dietary Supplements and Military Divers
... to ensure that they were safe and wholesome and that labeling was truthful and not misleading. The 1958 Food Additive Amendments to the Federal Food, Drug, and Cosmetic Act (FD&C Act) were a key regulation for ensuring safety of all new ingredients, including those used in dietary supplements. Howev ...
... to ensure that they were safe and wholesome and that labeling was truthful and not misleading. The 1958 Food Additive Amendments to the Federal Food, Drug, and Cosmetic Act (FD&C Act) were a key regulation for ensuring safety of all new ingredients, including those used in dietary supplements. Howev ...
Increase of Melanogenesis in the Presence of Fatty Acids
... active site. This conformational change enhances the affinity of active site towards its substrate which results in a more kinetically favorable reaction. On the other hand, conformational studies have revealed that, in addition to its active site, tyrosinase posses an effector site to which the eff ...
... active site. This conformational change enhances the affinity of active site towards its substrate which results in a more kinetically favorable reaction. On the other hand, conformational studies have revealed that, in addition to its active site, tyrosinase posses an effector site to which the eff ...
Title
... http://media.hsls.pitt.edu/media/molbiovideos/metacore1.swf http://media.hsls.pitt.edu/media/molbiovideos/metacore2.swf http://www.hsls.pitt.edu/molbio ...
... http://media.hsls.pitt.edu/media/molbiovideos/metacore1.swf http://media.hsls.pitt.edu/media/molbiovideos/metacore2.swf http://www.hsls.pitt.edu/molbio ...
The Minimal Nutritional Requirements of Organisms
... They would grow in the ammonia basal medium (6) plus nicotinic acid, with the addition of either glutamic acid, lactate, pyruvate, citrate or a-ketoglutarate. Glucose would not support growth; this was to be expected as the species does not ferment glucose, In simple mixtures of amino acids glutamic ...
... They would grow in the ammonia basal medium (6) plus nicotinic acid, with the addition of either glutamic acid, lactate, pyruvate, citrate or a-ketoglutarate. Glucose would not support growth; this was to be expected as the species does not ferment glucose, In simple mixtures of amino acids glutamic ...
Full-Text PDF
... of LUCA (Last Universal Cellular Ancestor), along with the universal conservation of the translation machinery [29]. However, LUCA certainly was not the first life form and most likely, not even the first cellular organism, only an evolutionary bottleneck. The early stages of cellular and especially ...
... of LUCA (Last Universal Cellular Ancestor), along with the universal conservation of the translation machinery [29]. However, LUCA certainly was not the first life form and most likely, not even the first cellular organism, only an evolutionary bottleneck. The early stages of cellular and especially ...
Specificity of the Organic Acid Activation of
... et al., 1994; Hoefnagel et al., 1995). Since partially purified AOX from Arum and soybean (Glycine max [L.]) cotyledons is stimulated and stabilized by pyruvate (Zhang et al., 1996), it is obvious that pyruvate reacts directly with the AOX protein. In soybean, it has been shown that pyruvate generat ...
... et al., 1994; Hoefnagel et al., 1995). Since partially purified AOX from Arum and soybean (Glycine max [L.]) cotyledons is stimulated and stabilized by pyruvate (Zhang et al., 1996), it is obvious that pyruvate reacts directly with the AOX protein. In soybean, it has been shown that pyruvate generat ...
Biosynthesis of Isoprenoids
... Ionization of the enzyme-bound LPP intermediate promotes cyclization to a sixmembered ring carbocation (the α-terpinyl cation), which may undergo additional electrophilic cyclizations, hydride shifts, or other rearrangements before the reaction is terminated by deprotonation of the carbocation or ca ...
... Ionization of the enzyme-bound LPP intermediate promotes cyclization to a sixmembered ring carbocation (the α-terpinyl cation), which may undergo additional electrophilic cyclizations, hydride shifts, or other rearrangements before the reaction is terminated by deprotonation of the carbocation or ca ...
SURVEY AND SUMMARY A profusion of upstream open reading
... at 16%, a 4-fold relative repression of main ORF initiation. When initiation of uORF translation leads to a physical barrier, for instance ribosome stalling, repression of main ORF initiation is, of course, much greater. Particular nascent peptide sequences, while still within the ribosome, can lead ...
... at 16%, a 4-fold relative repression of main ORF initiation. When initiation of uORF translation leads to a physical barrier, for instance ribosome stalling, repression of main ORF initiation is, of course, much greater. Particular nascent peptide sequences, while still within the ribosome, can lead ...
Modeling tRNA*s Translator Function
... • Go to the tRNA activity page at http://pdb101.rcsb.org/learn/resource/trna-activity-page or search for “Paper Model of tRNA – RCSB Protein Data Bank”. • Scroll down to section #3 titled, “Explore Atomic Structure of tRNA” and select the “Model color scheme” option for coloring the interactive mode ...
... • Go to the tRNA activity page at http://pdb101.rcsb.org/learn/resource/trna-activity-page or search for “Paper Model of tRNA – RCSB Protein Data Bank”. • Scroll down to section #3 titled, “Explore Atomic Structure of tRNA” and select the “Model color scheme” option for coloring the interactive mode ...
Carbohydrate-Based Mimetics in Drug Design: Sugar Amino Acids
... molecular recognition. In order to perform these functions, the correct folding of the biopolymers creating the active site is crucial, since any kind of interaction is observed only if the reactive groups are positioned in the correct spatial orientation to each other. Thus, the development of smal ...
... molecular recognition. In order to perform these functions, the correct folding of the biopolymers creating the active site is crucial, since any kind of interaction is observed only if the reactive groups are positioned in the correct spatial orientation to each other. Thus, the development of smal ...
PKU
... But the results are worth it--a healthy baby. Babies whose mothers have phe levels greater than 6 mg/dl may have babies with severe problems. The babies are born smaller than expected with small heads. The babies do not develop normally. Some of the babies have life-threatening heart problems. The c ...
... But the results are worth it--a healthy baby. Babies whose mothers have phe levels greater than 6 mg/dl may have babies with severe problems. The babies are born smaller than expected with small heads. The babies do not develop normally. Some of the babies have life-threatening heart problems. The c ...
Vitamins
... vitamin naturally by itself. This is vitamin D. All other vitamins that the body requires to function properly have to be derived from the diet. Lack of vitamins can have a serious affect on your health and may end in metabolic and other dysfunctions. ...
... vitamin naturally by itself. This is vitamin D. All other vitamins that the body requires to function properly have to be derived from the diet. Lack of vitamins can have a serious affect on your health and may end in metabolic and other dysfunctions. ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.