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Chapter 23
Chapter 23

... acetyl-CoA units and one propionyl-CoA. The 7 acetyl-CoA units are metabolized in the citric acid cycle with the following stoichiometry: 7 Acetyl-CoA + 14 O2 + 70 ADP + 70 Pi 7 CoA + 77 H2O + 14 CO2 + 70 ATP In producing the 7 acetyl-CoAs, 7 -carbons had to be oxidized, and only 6 of these by the ...
Characteristics of Phenylacrylic Acid Decarboxylase
Characteristics of Phenylacrylic Acid Decarboxylase

... of   undesired   side-­reactions   (e.g.,   decomposition,   isomerization,   racemization)   and   reduction   of   the   energy   burden   of   traditional   synthesis.   Enzymes   can   also   catalyze   almost   every   type   of   organic   reaction,   and   even   some   that  are  not  fou ...
Regulation of metabolism by dietary carbohydrates in two lines of
Regulation of metabolism by dietary carbohydrates in two lines of

... acids, relatively low number of insulin receptors, lack of inhibition of endogenous glucose production and poor hepatic lipogenesis from glucose were proved to be true (Mommsen and Plisetskaya, 1991; Hemre and Kahrs, 1997; Navarro et al., 1999; Panserat et al., 2000b; Enes et al., 2009); reduced per ...
Identification of proteins localized to the contractile vacuole of
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... osmotic stress 2.1 Contractile vacuoles and osmotic stress The contractile vacuole complex is an organelle that controls the intracellular water balance by accumulating and expelling excess water from the cell. Recent work has shown that the contractile vacuole is composed of a two compartment syste ...
Covalently Bonded Platinum(II) Complexes of [alpha]
Covalently Bonded Platinum(II) Complexes of [alpha]

... the N termini of a-amino acids (route B, Scheme 2). Thus, platination of 2 by the same procedure as that used for the pincer-bound a-amino acids (vide supra) led to the metalated aldehyde 5 in 76 % yield. Subsequently, 5 was treated with the respective a-amino esters in the presence of triethylamine ...
bme-biochem-3-kh-enzymes-9
bme-biochem-3-kh-enzymes-9

... Enzymes are mostly proteins They are highly specific to a reaction They catalyze many reactions including breaking down nutrients, storing and releasing energy, creating new molecules, and coordinating biological reactions. Enzymes use an active site, but can be affected by bonding at other areas ...
SNAREs
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Study: The Effects of Whey Protein Concentrate vs. Whey Protein
Study: The Effects of Whey Protein Concentrate vs. Whey Protein

... for better health.   Formulas containing whey protein concentrate can mix into fluids with the use of a  blender, hand blender or wire whisk. Whey protein concentrate contains less protein per gram than  ...
What is an enzyme? Function of enzymes
What is an enzyme? Function of enzymes

... enzyme cannot change conformations to the transition state. Therefore, enzymes must actually be complementary to the transition state so the reaction may proceed. This idea was researched by Haldane in 1930, and Linus Pauling in 1946. This idea led the Induced Fit theory, postulated by Daniel Ko ...
Advantages of compound-specific stable isotope
Advantages of compound-specific stable isotope

... Increasing interest in the ability of plants to take up amino acids has given rise to questions on the accuracy of the commonly used bulk method to measure and calculate amino acid uptake. This method uses bulk measurements of 13C and 15N enrichment in plant tissues after application of duallabelled ...
PFK - ePrints USM
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... They can be contrasted with thermophiles, which thrive at unusually hot temperatures. Despite the fact that a much greater proportion of the earth environment is cold rather than hot, much less is known about psychrophilic, cold-adapted microorganisms compared with thermophiles living at high temper ...
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Characterization and Surface Properties of Amino-Acid
Characterization and Surface Properties of Amino-Acid

... acid in solution before and after equilibration with CHA particles was measured by reaction with a dye and subsequent detection by spectrophotometry (see details below). The difference between these concentrations was taken as the amount adsorbed onto the CHA surface. For each of the amino acids 5 m ...
Targeting Acetyl-CoA Carboxylases: Small
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... (WO05113069 and US20050267221) from turmeric [80]. The natural products inhibit ACC activity by three modes. Curcumin phosphorylates and inactivates ACC via activating AMPK [81-83], Moiramide B and Andrimid act as a CT inhibitor, and other natural products inhibit the BC activity by interacting with ...
Lecture on BLAST
Lecture on BLAST

... custom position-specific score matrix • Bootstrapping results to find very related sequences • Megablast: • Search longer sequences with fewer differences • WU-BLAST: (Wash U BLAST) • Optimized, added features ...
CH - IS MU
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... The degradation of amino acids usually begins with deamination. However, transamination or oxidative deamination is not the first reaction in catabolism of eight amino acids: Serine and threonine are deaminated by dehydration, and histidine undergoes deamination by desaturation (both reactions were ...
Host Factors in the Replication of Positive
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... recruitment of LC3 to form a complete autophagosome-like vesicle. Subsequently, viral RdRp and other components of viral RC are recruited to these vesicles for viral replication. Importantly, poliovirus may have evolved to block subsequent maturation and degradation of these autolysosomal-like membr ...
CELLULAR RESPIRATION
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... CELLULAR RESPIRATION Energy-Releasing Pathways Anaerobic Definition Energy exchange occurring in the cell cytoplasm that does not use oxygen as the final electron acceptor. Aerobic Definition Energy exchange occurring in the mitochondria using oxygen as the final electron acceptor. ...
R Is for Arginine
R Is for Arginine

... the plasma l-arginine comes either from the diet or from recycling/proteolysis of cellular proteins, with a smaller fraction synthesized from other amino acids or recycled from l-arginine (at least in most cell types). The traffic of extracellular l-arginine (and other cationic amino acids, includin ...
R Is for Arginine Metabolism of Arginine Takes off Again, in New
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... the plasma l-arginine comes either from the diet or from recycling/proteolysis of cellular proteins, with a smaller fraction synthesized from other amino acids or recycled from l-arginine (at least in most cell types). The traffic of extracellular l-arginine (and other cationic amino acids, includin ...
cell biology - Bio
cell biology - Bio

... The overall theme for the book is the cell as the unit of life. We begin (Chapters 1–3) by describing the components of the cell as seen under the microscope. We then (Chapters 4–8) turn to the central dogma of molecular biology and describe how DNA is used to make RNA which in turn is used to make ...
Autophagy and pancreatitis
Autophagy and pancreatitis

Membrane transporters in a human genome
Membrane transporters in a human genome

1. Sources of cholesterol • diet ~ 30 % • biosynthesis ~ 70% 2. Types
1. Sources of cholesterol • diet ~ 30 % • biosynthesis ~ 70% 2. Types

... to cleave the pre-portion is inhibited. • When the free cholesterol falls, the protease is active and the pre- SREBP-1 is cleaved to liberate a fragment SREBP-1 that enters the nucleus. This factor then binds as a dimmer to SRE-1(sterol responsive element) of the HMG CoA reductase and LDL receptor g ...
Biochemical Tests of Renal Function
Biochemical Tests of Renal Function

... collect urine (usually for 24-hours) to determine the amount of creatinine that was removed from the blood over a given time interval.  Clearance is defined as the (hypothetical) quantity of blood or plasma completely cleared of a substance per unit of time.  The most frequently used clearance tes ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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