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Gene Expression
Gene Expression

... • Transcription moves coded information from DNA to the ribosome by creating an mRNA copy of a gene. • In translation, a ribosome “reads” the mRNA code and uses the information to assemble a chain of amino acids to make a protein. ...
gene expression - cloudfront.net
gene expression - cloudfront.net

... process, which begins with the arrival of the mRNA molecule at the ribosome. While the mRNA was being synthesized, tRNA molecules were uniting with their specific amino acids according to the activity of specific enzymes. The tRNA molecules then began transporting their amino acids to the ribosomes ...
Prepractical demo_SF_Class_2009
Prepractical demo_SF_Class_2009

...   Restriction Enzymes cleave DNA at a specific sequence (e.g., 5’ GAATTC 3’, 5’ CATATG 3’, 5’ CTGCAG 3’) Can you see anything unusual about these sequences? Why don’t they cleave the bacteria’s DNA?   We want an enzyme that will cleave one allele of the TAS2R38 sequence but not the other - need po ...
F. Mutation and Repair 1. Background on DNA Mutations
F. Mutation and Repair 1. Background on DNA Mutations

Biochemistry
Biochemistry

... have been linked and a water lost The bond holding the sugars together is a glycosidic bond Isomers—same chemical formula with different structures ...
Chapter 5
Chapter 5

... independent of DNA mutation but can also be the underlying cause 1. DNA damage is simply a chemical alteration to DNA, whereas DNA mutation is a change in one or more base pairs 2. DNA damage becomes DNA mutation when DNA replication proceeds without repairing the damage or by means of error-prone D ...
DNA Extraction Lab
DNA Extraction Lab

... Mashed Strawberry Filtered Strawberry Strawberry with Extraction Solution Strawberry with Isopropyl Alcohol DNA ...
What is the type of bond between Oxygen and Hydrogen in water?
What is the type of bond between Oxygen and Hydrogen in water?

... What type of bond involves the gain or loss of electrons? ...
Kein Folientitel
Kein Folientitel

... The first useful feature of pBR 322 is its size. As outlined before, a cloning vector ought to be less than 10 kb, to avoid problems such as DNA breakdown during purification. The size of 4363 bp means that not only the vector itself can be purified with ease, but so can recombinant DNA molecules co ...
Biomolecule Reading
Biomolecule Reading

... “R” groups are very small, others are large, and others form chains and rings. The sequence and shapes of the “R” groups control the shape and function of the protein. 27. How many different amino acids are there? 28. What part of the amino acid varies from one amino acid to another? 29. What determ ...
GENETICS VOCABULARY STUDY GUIDE Chapter 2 – section 3 1
GENETICS VOCABULARY STUDY GUIDE Chapter 2 – section 3 1

... 13. The  scientific  study  of  heredity.   ...
PowerPoint Presentation - Chapter 17 From Gene to Protein.
PowerPoint Presentation - Chapter 17 From Gene to Protein.

... sequence of amino acids making up the polypeptide chain.  It takes at least 300 nucleotides to code for a polypeptide that is 100 amino acids ...
Gene expression powerpoint
Gene expression powerpoint

... b. DNA is found in the nucleus c. Protein synthesis occurs in the ...
PCR-assay of intragenic DNA lesions induced by ionizing radiation
PCR-assay of intragenic DNA lesions induced by ionizing radiation

... 3. Project Summary: 3.1. Goal of Project: The goal of the Project is to detect the nature and location of DNA alterations induced by γ-rays and neutrons at the regulatory and coding parts of yellow gene Drosophila melanogaster. 3.2. Background and Topicality of Project: A large body of experimental ...
DNA VACCINES
DNA VACCINES

... improve the immunogenicity of DNA vaccines. The most frequently used approach is to co administer the antigen-expressing plasmid together with a plasmid encoding an immunostimulatory function, for example, the cDNA for a selected cytokine, chemokine, growth factor, or proapoptotic gene. The immunoge ...
Buffering Capacity
Buffering Capacity

... • To calculate ΔpHacid and ΔpHbase for a buffer, find the absolute value of the difference between the initial (0 drops) and final (30 drops) values for both probes. Record these values in table 1. • To determine and then rank the buffering capacities, calculate the ΔpHtotal by adding the ΔpHacid an ...
BIOL 212 General Genetics
BIOL 212 General Genetics

Protein Synthesis Lesson Plan
Protein Synthesis Lesson Plan

... Based on what we discussed previously, who can tell me how nitrogen bases along a gene serve as a genetic code? How is this gene sequence important in determining proteins? Whole group discussion: Students will brainstorm for a few minutes and present reasons. The teacher will display manipulatives, ...
Assignment #1
Assignment #1

... 3) A multicellular organism develops from a single zygote, and its phenotype(12) depends on its genotype(13), which is established at fertilization(14). a. How to predict the probable outcome of phenotypes in a genetic cross from the genotypes of the parents and mode of inheritance (autosomal(15) or ...
Whittier Union High School District
Whittier Union High School District

... 50. The process of information encoded in DNA being rewritten as mRNA is called transcription. 51. Determine the mRNA sequence that would be produced from this original template DNA strand. DNA = 5` A T G G C T A ...
File
File

... base pairing • The importance of such complementary base pairing is that each strand of DNA can act as template to direct the synthesis of other strand similar to its complementary one. • Thus nucleic acids are uniquely capable of directing their own self replication. • The diameter of the helix cou ...
Guide to preparation of liquid biopsies for nucleic acid extraction
Guide to preparation of liquid biopsies for nucleic acid extraction

... 3.   Don’t waste your time on integrity and concentration. Nucleic acid extraction from liquid biopsies typically results in low amounts of fragmented RNA or DNA. As such RNA/DNA concentration measurements are generally not reliable, we standardize our workflows based on volume input. Furthermore, w ...
CHAPTER 3 THE CHEMISTRY OF ORGANIC MOLECULES
CHAPTER 3 THE CHEMISTRY OF ORGANIC MOLECULES

... transport, defense, regulation, and motion. Proteins are polymers of amino acids. A polypeptide is a long chain of amino acids joined by peptide bonds. An amino acid consists of a hydrogen, a carboxyl group, an amino group, and a variable R group that are all attached to a central carbon atom. There ...
1.1 Biological Background
1.1 Biological Background

... anticodon. The anticodon is a sequence of three RNA bases. On the other side, the tRNA holds an amino acid. The many-to-one mapping from anticodons to amino acids de ned by tRNA molecules is the universal genetic code (see below). ...
Document
Document

... base) daughter strands from the molecule being sequenced that differ by one nucleotide in length, and 2) separation of the fragments by electrophoresis. The sequence then is read from the positions of consecutive fragments on the gel. Termination at each base is accomplished using dideoxyribonucleos ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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