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... its other end a specific sequence of three nucleotides 2) Ribosome (rRNAs) latches on one end of the mRNA trundles through it and picks up tRNAs loaded with amino acids 3) In the ribosome tRNAs attach to a matching segment of the mRNA. The amino acids link together to extend the growing protein chai ...
Semester 2 Final Review
Semester 2 Final Review

... 28. What happens during translation and transcription? What happens first? 29. Use your genetic code chart to translate the mRNA into amino acids: AUGUUUCAGUGA 30. Why is tRNA important in translation? 31. How many amino acids does this DNA sequence represent: TAAAGGCCC? 32. What is the ratio of A:T ...
CH. 12.3 : DNA, RNA, and Protein
CH. 12.3 : DNA, RNA, and Protein

... The Genetic Code The nucleotide sequence transcribed from DNA to a strand of messenger RNA acts as a genetic message, the complete information for the building of a protein. Virtually all organisms share the same genetic code. ...
Examination 3
Examination 3

... The usual enzymes can not extend the new DNA strand The telomere prevents erosion of chromosome ends during rounds of replication Uses RNA, made of protein, to add to the chromosome Why is telomerase an important enzyme? What does it use as a template for adding additional DNA bases? Base pairing (F ...
Lecture 27
Lecture 27

... • Leading strand is continuously copied from the 3’ to 5’ parental template in the 5’ to 3’ direction • Lagging strand is discontinuously replicated in pieces from the 5’ to 3’ parental strands. • E. coli has 2 DNA polymerases necessary for survival. DNA polymerase III (Pol III) synthesizes the lead ...
BIOTECHNOLOGY -intentional manipulation of
BIOTECHNOLOGY -intentional manipulation of

... • Genetically modified organisms (GMOs) are organisms whose genes have been directly manipulated by scientists, often by inserting or deleting one or more genes. Inserted genes are typically from another species. ...
Genetic Engineering
Genetic Engineering

... to form recombinant DNA. 3. The recombinant DNA is inserted into the new organism, which uses the gene. ...
0101BWhat characterizes a prokaryotic cell
0101BWhat characterizes a prokaryotic cell

... d) the number of isotopes of the atom b) the number of electrons in the outermost shell e) none of the above is correct c) the number of neutrons in the nucleus __21) The atomic number of nitrogen is 7. 15N is heavier than 14N because 15N has: a) eight protons b) eight neutrons c) seven protons d) s ...
Exempt Dealing Application Form - Macquarie University
Exempt Dealing Application Form - Macquarie University

... 4. Describe the host organism or tissue to be used – include the genus, species and strain where applicable. If not a commonly used laboratory strain, include the name of the strain from which it is derived. ...
enzymes!!!!! - Highline Public Schools
enzymes!!!!! - Highline Public Schools

... Lipo=fat  Also known as fats.  Lipids are mostly long chains of carbon and hydrogen.  Some also have a little oxygen.  Examples: ...
Lecture ** - Telomeres
Lecture ** - Telomeres

... a) 5'->3' DNA replication of "lagging strand" b) How does cell discriminate between DNA double-strand breaks (which are also “ends”) and the natural ends of chromosomes? The solution: telomeres! a) Telomere structure: Repetitive DNA sequences at ends of chromosomes • telomeric heterochromatin (gene ...
Enzyme Mechanisms - Illinois Institute of Technology
Enzyme Mechanisms - Illinois Institute of Technology

... the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesis because the 3’ position is blocked. See figs. 11.3 & 11.4 for how these are read out ...
genes notes
genes notes

... Can be arranged in an infinite number of ways. Within these molecules is the genetic code that determines all the characteristics of an organism. Different segments of the chromosomes control different traits that are expressed in the organism. ...
Word Doc - SEA
Word Doc - SEA

File - Mrs. Badger`s Honors Biology Class
File - Mrs. Badger`s Honors Biology Class

... replication (nucleus) transcription (nucleus) translation (cytoplasm) Contains the sugar ribose Has the bases A, C, G, and T Typically single-stranded RNA polymerase A large transcription complex, including RNA polymerase and other proteins, assembles at the start of a gene and begins to unwind the ...
Chemistry-Chapter 2 Lecture Notes Page
Chemistry-Chapter 2 Lecture Notes Page

... - Primary Level = order of amino acids in polypeptide - Secondary Level = twisting & folding of chain, held by hydrogen bonds - Tertiary Level = 3 Dimensional shape, determine function - Quaternary Level = multiple polypeptide chains ...
MBLG1001 Lecture 9 The Flow of Genetic Information Replication
MBLG1001 Lecture 9 The Flow of Genetic Information Replication

... • The DNA one cell division after medium change would be composed of H:H and L:L in equal proportions. • In the second generation there should be 3 L:L to 1 H:H. • The third generation..7 L:L and 1 H:H ...
CHAPTER 14
CHAPTER 14

... mRNA molecules would bind to this column because they have a polyA tail. The string of adenine nucleotides in the polyA tail is complementary to stretch of thymine in the poly-dT column, so the two would hydrogen bond to each other. To purify mRNAs, one begins with a sample of cells; the cells need ...
Restriction fragment length polymorphism
Restriction fragment length polymorphism

... Tools and technique of molecular biology ...
Bacterial Transformation
Bacterial Transformation

... Vehicles for foreign DNA Plasmid DNA usually contains genes for one or more traits that may be beneficial to bacterial survival Bacteria can transfer plasmids back and forth, allowing them to share beneficial genes Allows for adaptation to new environments Recent occurrence of bacterial antibiotic r ...
Only One Strand of DNA Is Translated
Only One Strand of DNA Is Translated

... separated into two strands during the transcription stage of protein synthesis, only one strand was copied by mRNA to make a protein. ...
Nucleoside Phosphoramidate Monoesters: Potential
Nucleoside Phosphoramidate Monoesters: Potential

... are coupled ...
RODENT GENOTYPING The proper identification of transgenic
RODENT GENOTYPING The proper identification of transgenic

... genotype is determined by analysis of DNA extracted from tissues of young mice. Analysis by the Polymerase Chain Reaction (PCR) requires the least amount of DNA. DNA for PCR analysis can be obtained by a variety of techniques that vary in terms of the discomfort the animal may experience. When anima ...
File
File

... Under natural conditions, plasmids are transmitted to a new host by a process called bacterial conjugation. In the laboratory, plasmid DNA can be introduced into bacteria by the artificial process of transformation. In this process, bacteria are treated with divalent cations (Ca2+) to make them temp ...
Honors_Genetics_B_Student_Notes
Honors_Genetics_B_Student_Notes

... - used to improve quality of livestock and crops - used to improve disease resistance to increase crop production (ex: Bt corn) ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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