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recombinant DNA. Lesson Overview
recombinant DNA. Lesson Overview

Remember: Condensation makes bonds: Hydrolysis breaks bonds.
Remember: Condensation makes bonds: Hydrolysis breaks bonds.

... • “Organic compounds are based on carbon and are found in living things.” (1) 2. List three carbon-containing groups or molecules that are not organic. There are many organic molecules in living things. The same (or very similar) molecules are used in many different living things for the same purpos ...
10/24 - bio.utexas.edu
10/24 - bio.utexas.edu

... several different methods all involving perturbing the bacterial membrane: •Electroporation ...
Concept of DNA and RNA
Concept of DNA and RNA

... composed of DNA (or RNA) inside a protein coat/shell called a capsid. It was also known that viruses replicate by taking over the host cell metabolic functions to make more virus. We are used to thinking and talking about viruses, which invade our bodies and make us sick, but there are other, differ ...
copyrighted material
copyrighted material

... that consist of a single unit (monomer) repeated many times. Four of carbon’s six electrons are available to form bonds with other atoms. Thus, you will always see four lines connecting a carbon atom to other atoms, each line representing a pair of shared electrons (one electron from carbon and one ...
The hunt for dim mutants - University of Oregon (SPUR)
The hunt for dim mutants - University of Oregon (SPUR)

... So far I have tested about 120 N51 cross progeny for DNA methylation. Of those 120, only about 30 look to have the genotype. There is a problem with this method, it takes too long to gather enough progeny for testing. We are getting false positives. There needs to be a faster way to obtain mutants f ...
Chapter10_Outline
Chapter10_Outline

... Genomics and Proteomics • The field of genomics deals with the DNA sequence, organization, function, and evolution of genomes • Proteomics aims to identify all the proteins in a cell or organism including any posttranslationally modified forms, as well as their cellular localization, functions, and ...
1 - Biology Mad
1 - Biology Mad

... One strand labelled with a radioactive phosphorus atom, 32 P Piece of DNA The strands are separated and the one ...
Exam 1
Exam 1

... ---The A280 of the undiluted protein solution is 30 x 0.25 = 7.5. This is at 10-fold more concentrated than desired. Mix 0.1 mL protein solution and 0.9 mL of water to give an A280 = 0.75. ---At pH 10 the amino terminus is largely dissociated and uncharged, therefore, there are no strictly electrost ...
(HSV) PCR, CSF
(HSV) PCR, CSF

... HSV DNA may not be detectable in the early acute stages of the CNS disease. In addition, in some cases, after initial detection (positive result),HSV DNA may be present in CSF specimens for 3-4 weeks after initial presentation of symptoms. DNA levels may fall to undetectable levels with time. •Speci ...
Nonstandard amino acids are found in modified proteins
Nonstandard amino acids are found in modified proteins

... For a protein with 100 aa, number of possible aa sequences = 20100 ≈ 10130 For comparison, there are ~1080 atoms in the (observable) universe! Additional variability can come from: • Variation in chain length • Variation in number of chains • Protein modifications • Binding of prosthetic groups ...
Information. How to bring your samples
Information. How to bring your samples

... IIBm Genomics Core Facility (SQP) Gemma Rodríguez- Tarduchy, PhD. SQP Headmaster. Date: 26/07/14. Rev: 00 ...
Do microRNAs induced by Viral Hemorrhagic Septicemia virus in
Do microRNAs induced by Viral Hemorrhagic Septicemia virus in

... septicemia virus (VHSV) infection. These miRNAs were also up regulated in the liver and muscle (vaccination site) of fish vaccinated with a DNA vaccine expressing the VHSV glycoprotein gene. Recent studies further indicate that the expression of these miRNAs is induced by interferons. In order to an ...
chapter 23
chapter 23

... of cellulose make up plant cell walls. More than 50% of the total organic matter in the world is cellulose. People cannot digest cellulose, but when we eat fiber, which is cellulose, it speeds the movement of food through the digestive tract. Microorganisms that can digest cellulose are present in t ...
Amino acids have many roles in living organisms
Amino acids have many roles in living organisms

... For a protein with 100 aa, number of possible aa sequences = 20100 ≈ 10130 For comparison, there are ~1080 atoms in the (observable) universe! Additional variability can come from: • Variation in chain length • Variation in number of chains • Protein modifications • Binding of prosthetic groups ...
Foundations in Microbiology
Foundations in Microbiology

... results from the combination of two or more atoms • Compounds – molecules that are combinations of 2 or more different elements • Chemical bonds – when 2 or more atoms share, donate or accept electrons to form molecules and compounds – 3 types: covalent, ionic, and hydrogen ...
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File

... molecules that compose living organisms. Clarification: Carbohydrates are limited to general structural formulas of simple sugars and polymers of those sugars and their functions as short- and long-term energy storage molecules as well as structural components of cell walls. Lipids are limited to ge ...
BI0I 121 cel]
BI0I 121 cel]

... The 4 nucleic acid bases combine in 2-letter combinations that define different amino acids. The 4 nucleic acid bases combine in 3-letter sequences that define different amino acids. Triplets of the 2-letter nucleic acid bases are translated into the 20 different amino acids. The 4 bases each specif ...
15 N
15 N

... varies from species to species  all 4 bases not in equal quantity  bases present in characteristic ratio ...
Ninth Grade Biology Unit 3 – Growth and Heredity Asexual and
Ninth Grade Biology Unit 3 – Growth and Heredity Asexual and

... Differentiated Instructional Strategies: (References: Science Formative Assessment by Page Keeley; Differentiated Instructional Strategies by Gayle Gregory and Carolyn Chapman) o Questionnaire: Give students a short questionnaire to see what they know about genetic engineering (cloning, stem cells). ...
Heredity – the passing of traits from parents to offspring
Heredity – the passing of traits from parents to offspring

... Heredity – the passing of traits from parents to offspring. Genetics – the study of heredity DNA – cellular material with all the information about what traits a living thing may have Chromosome – thread like structures that organize DNA, each chromosome is a long DNA molecule (about 1.5 meters!) Ge ...
DNA Structure Review
DNA Structure Review

... • The smaller DNA fragments will move at a faster rate on the gel plate than the larger ones. • The fragments are then transferred to a nylon membrane in a process called Southern blotting. • To visualize the RFLPs, the nylon sheet is treated with radioactive probes containing a base sequence comple ...
Vectors
Vectors

... Human proteins produce fewer side effects than proteins from other animals (e.g. pork insulin vs. human insulin) -- Hormones or hormone-like compounds -- Enzymes ...
MutaGEL® r-Vitamin D3
MutaGEL® r-Vitamin D3

... The kit MutaGEL r-vitamin D3 contains a set of primer for amplification of the specific DNA sequence within the human vitamin D3 receptor gene VD3R. Amplificates of variing genotypes (start codon polymorphism) are characterized by subsequent specific restriction enzyme digestion. The rare variant (f ...
Carbon Macromolecules
Carbon Macromolecules

... 2. One carbon atom can bond to another carbon atom, which gives carbon the ability to form chains that are almost unlimited in length. Carbon has the ability to form millions of different large and complex structures. ...
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Nucleic acid analogue



Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.
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