Tumor metastasis-associated human MTA1 gene and its MTA1
... Using differential cDNA library screening techniques based on metastatic and nonmetastatic rat mammary adenocarcinoma cell lines, we previously cloned and sequenced the metastasis-associated gene mta1. Using homology to the rat mta1 gene, we cloned the human MTA1 gene and found it to be over-express ...
... Using differential cDNA library screening techniques based on metastatic and nonmetastatic rat mammary adenocarcinoma cell lines, we previously cloned and sequenced the metastasis-associated gene mta1. Using homology to the rat mta1 gene, we cloned the human MTA1 gene and found it to be over-express ...
cis-Regulatory Elements and trans-Acting Factors
... negative regulatory element could bind a specific nuclear protein, present in the submandibular gland, resulting in the inhibition of Reni expression in this tissue.4 Second, the high expression of submandibular Ren2 could be due to the nonfunctionality of the negative regulatory element.4 Recently, ...
... negative regulatory element could bind a specific nuclear protein, present in the submandibular gland, resulting in the inhibition of Reni expression in this tissue.4 Second, the high expression of submandibular Ren2 could be due to the nonfunctionality of the negative regulatory element.4 Recently, ...
1 Depleting gene activities in early Drosophila embryos
... signaling (Peifer et al., 1991) (Figure 3). Similarly, most F1 embryos (95%) from mattub-Gal4 females crossed to UAS-shRNA-N (line HMS0009), but not from the reverse cross, showed a neurogenic phenotype (Figure 3). Note that the VALIUM22 line against N (GL00092) showed lower F1 lethality (10%), most ...
... signaling (Peifer et al., 1991) (Figure 3). Similarly, most F1 embryos (95%) from mattub-Gal4 females crossed to UAS-shRNA-N (line HMS0009), but not from the reverse cross, showed a neurogenic phenotype (Figure 3). Note that the VALIUM22 line against N (GL00092) showed lower F1 lethality (10%), most ...
PDF - Oxford Academic - Oxford University Press
... dominant optic neuropathy but no spasticity linked to the SPG7 locus. We identified two SPG7 mutations in 23/134 spastic patients, 21% of the patients selected according to phenotype but only 8% of those referred directly. Our results confirm the pathogenicity of Ala510Val, which was the most freque ...
... dominant optic neuropathy but no spasticity linked to the SPG7 locus. We identified two SPG7 mutations in 23/134 spastic patients, 21% of the patients selected according to phenotype but only 8% of those referred directly. Our results confirm the pathogenicity of Ala510Val, which was the most freque ...
DROSOPHILA: GENETICS MEETS BEHAVIOUR
... Box 1 | Behaviour as a phenotype for genetic analysis Behaviour, arguably one of the most complex phenotypes, has been considered to be the action of an animal in response to its internal and external environment. However, this definition of behaviour is vague and not limited to behavioural phenotyp ...
... Box 1 | Behaviour as a phenotype for genetic analysis Behaviour, arguably one of the most complex phenotypes, has been considered to be the action of an animal in response to its internal and external environment. However, this definition of behaviour is vague and not limited to behavioural phenotyp ...
Nucleic Acids Research, 32: D489-D492 (2004).
... that Alu subfamilies have originated through successive waves of ®xation from sequential small subsets of active Alu sequences. The oldest Alu-related elements are the monomeric FAM, FRAM and FLAM sequences. The oldest Alu dimeric subfamilies are Alu-Jo and Alu-Jb, estimated to be ~80 million years ...
... that Alu subfamilies have originated through successive waves of ®xation from sequential small subsets of active Alu sequences. The oldest Alu-related elements are the monomeric FAM, FRAM and FLAM sequences. The oldest Alu dimeric subfamilies are Alu-Jo and Alu-Jb, estimated to be ~80 million years ...
The Genome of a Mongolian Individual Reveals
... and population data are lacking. They are increasingly necessary to explore characteristics of population evolution, disease, and personal healthcare. In this study, we sequenced the genome of a representative Mongolian male individual with high coverage (>100) by using the next sequencing technolo ...
... and population data are lacking. They are increasingly necessary to explore characteristics of population evolution, disease, and personal healthcare. In this study, we sequenced the genome of a representative Mongolian male individual with high coverage (>100) by using the next sequencing technolo ...
Mutation, Mutagens, and DNA Repair
... the integrity of bacterial DNA is confirmed by the observation that dam- strains of E. coli have increased rates of spontaneous mutation. The mismatch repair system can act at a distance - in other words, a mismatch can be repaired even though the nearest hemimethylated site is 1000 bp away. Repair ...
... the integrity of bacterial DNA is confirmed by the observation that dam- strains of E. coli have increased rates of spontaneous mutation. The mismatch repair system can act at a distance - in other words, a mismatch can be repaired even though the nearest hemimethylated site is 1000 bp away. Repair ...
Genetic polymorphism of epoxide hydrolase and glutathione S-transferase in COPD S-L. Cheng
... Hardy-Weinberg equilibrium was tested for all polymorphisms and no obvious deviation was found. On multivariate analysis of the four genes, the frequency of the GSTM1-null genotype was significantly higher in the patients with COPD than in the control subjects (61.4 versus 42.5%) (table 2). The odds ...
... Hardy-Weinberg equilibrium was tested for all polymorphisms and no obvious deviation was found. On multivariate analysis of the four genes, the frequency of the GSTM1-null genotype was significantly higher in the patients with COPD than in the control subjects (61.4 versus 42.5%) (table 2). The odds ...
Gene Expression
... Pairing of complementary bases is the key to the transfer of information from DNA to RNA and from RNA to protein Polarities of DNA, RNA, and polypeptides help guide the mechanisms of gene expression Gene expression requires input of energy and participation of specific proteins and macromolecular as ...
... Pairing of complementary bases is the key to the transfer of information from DNA to RNA and from RNA to protein Polarities of DNA, RNA, and polypeptides help guide the mechanisms of gene expression Gene expression requires input of energy and participation of specific proteins and macromolecular as ...
Bone Marrow Failure
... Explanation: Longstanding cytopenias with idiopathic macrocytosis are frequent features of inherited marrow failure syndromes. Leukoplakia with an increased risk of squamous cell carcinoma is associated with dyskeratosis congenita and the family history is consistent with an autosomal dominant patt ...
... Explanation: Longstanding cytopenias with idiopathic macrocytosis are frequent features of inherited marrow failure syndromes. Leukoplakia with an increased risk of squamous cell carcinoma is associated with dyskeratosis congenita and the family history is consistent with an autosomal dominant patt ...
How dormant origins promote complete genome replication
... With these considerations in mind, we recently modelled the behaviour of origin activation within a single 250 kb origin cluster [41]. Origins were assigned a certain initiation probability per unit time and were then activated stochastically during S phase (Figure 4a). Model parameters (mean origin ...
... With these considerations in mind, we recently modelled the behaviour of origin activation within a single 250 kb origin cluster [41]. Origins were assigned a certain initiation probability per unit time and were then activated stochastically during S phase (Figure 4a). Model parameters (mean origin ...
Mechanisms and impact of genetic recombination in the evolution of
... base sequence are known as random mutations. The rate at which random mutations occur varies between bacteria due to the differences in fidelity of DNA polymerases. Spontaneous mutations are either maintained or discarded in the population depending on the fitness cost of the new genotypes [26]. Advan ...
... base sequence are known as random mutations. The rate at which random mutations occur varies between bacteria due to the differences in fidelity of DNA polymerases. Spontaneous mutations are either maintained or discarded in the population depending on the fitness cost of the new genotypes [26]. Advan ...
Gene Section ALK (anaplastic lymphoma kinase) Atlas of Genetics and Cytogenetics
... NPM1 fused to the 562 C-term aminoacids of ALK (i.e. composed of the oligomerization domain and the metal binding site of NPM1, and the entire cytoplasmic portion of ALK); no apparent expres-sion of the ALK/NPM1 counterpart. Characteristic localisation both in the cytoplasm and in the nucleus, due t ...
... NPM1 fused to the 562 C-term aminoacids of ALK (i.e. composed of the oligomerization domain and the metal binding site of NPM1, and the entire cytoplasmic portion of ALK); no apparent expres-sion of the ALK/NPM1 counterpart. Characteristic localisation both in the cytoplasm and in the nucleus, due t ...
Test Info Sheet
... 3. Genetic counseling 4. Prenatal diagnosis for known familial mutation(s) in at-risk pregnancies Methods: Using genomic DNA, coding exons and flanking splice junctions of the genes on this panel are enriched using a proprietary targeted capture method developed by GeneDx. The products are sequenced ...
... 3. Genetic counseling 4. Prenatal diagnosis for known familial mutation(s) in at-risk pregnancies Methods: Using genomic DNA, coding exons and flanking splice junctions of the genes on this panel are enriched using a proprietary targeted capture method developed by GeneDx. The products are sequenced ...
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... and Bourgeois, supra), and is itself rapidly converted to chromosome to improve control of heterologous gene glucose and galactose. expression from a lac operated promoter (as long as a Another control element of the lac operon is catabo promoter is present to drive lacIZYA transcription). It lite r ...
... and Bourgeois, supra), and is itself rapidly converted to chromosome to improve control of heterologous gene glucose and galactose. expression from a lac operated promoter (as long as a Another control element of the lac operon is catabo promoter is present to drive lacIZYA transcription). It lite r ...
Conspiracy of silence among repeated transgenes
... comparable impact in agriculture and medicine. Researchers usually assume that an integrated transgene’s behavior reflects normal influences at the site of insertion. However, genomes have mechanisms for recognizing potentially threatening sequence elements, like transposons, and silencing them. Cyt ...
... comparable impact in agriculture and medicine. Researchers usually assume that an integrated transgene’s behavior reflects normal influences at the site of insertion. However, genomes have mechanisms for recognizing potentially threatening sequence elements, like transposons, and silencing them. Cyt ...
Article Why There Are No Essential Genes on
... are differences between functions coded for by mobile genes and those in the “core” genome and that these differences can be seen between plasmids and chromosomes. In particular, it has been suggested that essential genes, such as those involved in the formation of structural proteins or in basic me ...
... are differences between functions coded for by mobile genes and those in the “core” genome and that these differences can be seen between plasmids and chromosomes. In particular, it has been suggested that essential genes, such as those involved in the formation of structural proteins or in basic me ...
Introduction: - Statistical Science
... Figure SF1. This figure compares the empirical null and observed distributions in the Diabetes example for a randomly generated collection of 1000 gene sets (top) and the functional gene sets (S2 database) before and after normalization (i.e., area under positive and negative density distributions e ...
... Figure SF1. This figure compares the empirical null and observed distributions in the Diabetes example for a randomly generated collection of 1000 gene sets (top) and the functional gene sets (S2 database) before and after normalization (i.e., area under positive and negative density distributions e ...
The Large Loop Repair and Mismatch Repair Pathways
... in the RAD1-dependent LLR pathway springs from the known enzymatic roles of those proteins during mitotic DNA repair and the observed effects on meiotic recombination and DNA repair upon deletion of the LLR genes (Kirkpatrick and Petes 1997). Given the characterized activities of Rad1/10p and Msh2/3 ...
... in the RAD1-dependent LLR pathway springs from the known enzymatic roles of those proteins during mitotic DNA repair and the observed effects on meiotic recombination and DNA repair upon deletion of the LLR genes (Kirkpatrick and Petes 1997). Given the characterized activities of Rad1/10p and Msh2/3 ...
Click
... On this new results page it should be easily possible to locate the results for BRCA1 and all other OMIM accessions that contain BRCA1 in the name or file. Recall that we looked at other resources that could be obtained from OMIM. Most important of these is the ENTREZ Gene page. This page can be di ...
... On this new results page it should be easily possible to locate the results for BRCA1 and all other OMIM accessions that contain BRCA1 in the name or file. Recall that we looked at other resources that could be obtained from OMIM. Most important of these is the ENTREZ Gene page. This page can be di ...
Exome sequencing as a tool for Mendelian disease gene discovery
... The systematic identification of rare alleles (that is, with a minor allele frequency (MAF) ≤ 1%) associated with common traits typically requires resequencing instead of genotyping83 and has therefore been challenging. Such studies have largely been limited to assessing rare variants that have been ...
... The systematic identification of rare alleles (that is, with a minor allele frequency (MAF) ≤ 1%) associated with common traits typically requires resequencing instead of genotyping83 and has therefore been challenging. Such studies have largely been limited to assessing rare variants that have been ...
Oncogenomics
Oncogenomics is a relatively new sub-field of genomics that applies high throughput technologies to characterize genes associated with cancer. Oncogenomics is synonymous with ""cancer genomics"". Cancer is a genetic disease caused by accumulation of mutations to DNA leading to unrestrained cell proliferation and neoplasm formation. The goal of oncogenomics is to identify new oncogenes or tumor suppressor genes that may provide new insights into cancer diagnosis, predicting clinical outcome of cancers, and new targets for cancer therapies. The success of targeted cancer therapies such as Gleevec, Herceptin, and Avastin raised the hope for oncogenomics to elucidate new targets for cancer treatment.Besides understanding the underlying genetic mechanisms that initiates or drives cancer progression, one of the main goals of oncogenomics is to allow for the development of personalized cancer treatment. Cancer develops due to an accumulation of mutations in DNA. These mutations accumulate randomly, and thus, different DNA mutations and mutation combinations exist between different individuals with the same type of cancer. Thus, identifying and targeting specific mutations which have occurred in an individual patient may lead to increased efficacy of cancer therapy.The completion of the Human Genome Project has greatly facilitated the field of oncogenomics and has increased the abilities of researchers to find cancer causing genes. In addition, the sequencing technologies now available for sequence generation and data analysis have been applied to the study of oncogenomics. With the amount of research conducted on cancer genomes and the accumulation of databases documenting the mutational changes, it has been predicted that the most important cancer-causing mutations, rearrangements, and altered expression levels will be cataloged and well characterized within the next decade.Cancer research may look either on the genomic level at DNA mutations, the epigenetic level at methylation or histone modification changes, the transcription level at altered levels of gene expression, or the protein level at altered levels of protein abundance and function in cancer cells. Oncogenomics focuses on the genomic, epigenomic, and transcript level alterations in cancer.