Genomic Analysis of Arabidopsis Thaliana
... In addition to the many advantages described above, the rapid rise of Arabidopsis as a preferred plant model is based upon its small genome size and few repetitive sequences. The framework for Arabadopsis genomics was set by early kinetic analyses of total Arabidopsis DNA in 1984. Most of the genome ...
... In addition to the many advantages described above, the rapid rise of Arabidopsis as a preferred plant model is based upon its small genome size and few repetitive sequences. The framework for Arabadopsis genomics was set by early kinetic analyses of total Arabidopsis DNA in 1984. Most of the genome ...
CHAPTER 21 Chromosomal Mutations
... iii. Chemicals. iv.Transposable elements. v. Errors in recombination. b. Deletions do not revert, because the DNA is missing. ...
... iii. Chemicals. iv.Transposable elements. v. Errors in recombination. b. Deletions do not revert, because the DNA is missing. ...
A Genetic Linkage Map of Mouse Chromosome 10
... possible that the mouse 3' cDNA probe detected a Bcr-related locus in the mouse rather than the structural gene. We mapped the mouse Bcr cDNA probe using multiple restriction enzymes (data not shown), but all polymorphisms segregated to the same locus on chromosome 10. Probes derived from exon 1 of ...
... possible that the mouse 3' cDNA probe detected a Bcr-related locus in the mouse rather than the structural gene. We mapped the mouse Bcr cDNA probe using multiple restriction enzymes (data not shown), but all polymorphisms segregated to the same locus on chromosome 10. Probes derived from exon 1 of ...
Figures and figure supplements
... explaining the somewhat high correlation with H3K27me3 (see Figure 1—source data 4). However, this does not affect the conclusions of the paper in any way. (B) Adult chromatin state annotations on a predominantly silenced region. For the definition of chromatin states see Figure 1A. Coding genes (pu ...
... explaining the somewhat high correlation with H3K27me3 (see Figure 1—source data 4). However, this does not affect the conclusions of the paper in any way. (B) Adult chromatin state annotations on a predominantly silenced region. For the definition of chromatin states see Figure 1A. Coding genes (pu ...
Regulation of Bacterial Conjugation in Microaerobiosis by
... strain to strain using a cotransducible Tn10dCm element, as previously described (Camacho et al. 2005b). Whenever a Tn10dCm insertion was linked (.60%) to the point mutation, the boundaries of the Tn10dCm element were sequenced (Torreblanca et al. 1999). Primers for chromosome walking and serial DNA ...
... strain to strain using a cotransducible Tn10dCm element, as previously described (Camacho et al. 2005b). Whenever a Tn10dCm insertion was linked (.60%) to the point mutation, the boundaries of the Tn10dCm element were sequenced (Torreblanca et al. 1999). Primers for chromosome walking and serial DNA ...
DNA phosphorothioation inStreptomyces lividans: mutational
... complementation experiments using plasmids expressing individual Dnd proteins. This was achieved by the construction of individual dnd gene expression plasmids using pHZ1272 [18], an E. coli-Streptomyces shuttle expression vector derived from pIJ6021 with a strong thiostrepton-inducible PtipA promot ...
... complementation experiments using plasmids expressing individual Dnd proteins. This was achieved by the construction of individual dnd gene expression plasmids using pHZ1272 [18], an E. coli-Streptomyces shuttle expression vector derived from pIJ6021 with a strong thiostrepton-inducible PtipA promot ...
MicroRNA Analysis
... critical to stop the stem-cell-like divisions of seam cells and induce their fully differentiated state. Reduced let-7 expression is associated with human cancers and cancer stem cells, thus suggesting that let-7 in humans also promotes terminal differentiation and is a tumor suppressor. • 1998-Fire ...
... critical to stop the stem-cell-like divisions of seam cells and induce their fully differentiated state. Reduced let-7 expression is associated with human cancers and cancer stem cells, thus suggesting that let-7 in humans also promotes terminal differentiation and is a tumor suppressor. • 1998-Fire ...
In Silico method for identification of MHC class I
... Before the algorithm was applied to mammalian species in which the CD1 molecules had never been studied before, we first determined the sensitivity and accuracy of the developed method. To do this, the second and third exons of human CD1a, CD1b, CD1c and CD1d were used as input for ...
... Before the algorithm was applied to mammalian species in which the CD1 molecules had never been studied before, we first determined the sensitivity and accuracy of the developed method. To do this, the second and third exons of human CD1a, CD1b, CD1c and CD1d were used as input for ...
Oliver, B., Kim, Y.-J., and Baker, B. S.
... Steinmann-Zwicky, 1992). The ovaries of wild-type females contain 16-cell cysts of germ-line cells each of which arises from a stem cell by four nuclear divisions with incomplete cytokinesis (King, 1970). As each cyst matures, one cyst cell becomes an oocyte while the remaining fifteen become polypl ...
... Steinmann-Zwicky, 1992). The ovaries of wild-type females contain 16-cell cysts of germ-line cells each of which arises from a stem cell by four nuclear divisions with incomplete cytokinesis (King, 1970). As each cyst matures, one cyst cell becomes an oocyte while the remaining fifteen become polypl ...
... Differential gene expression results in cell diversity, although how different cell identities are established early in development and maintained throughout life is still poorly understood. Most of the transcription factors required for early developmental decisions are expressed transiently, but t ...
Gene Duplication - Semantic Scholar
... (or genome) duplication. Zhang 2003 summarizes the main features of these mechanisms. Kaessmann, et al. 2009 provides detailed information about retroposition-mediated gene duplication. Most genetics textbooks, including Klug, et al. 2009, describe detailed mechanisms ...
... (or genome) duplication. Zhang 2003 summarizes the main features of these mechanisms. Kaessmann, et al. 2009 provides detailed information about retroposition-mediated gene duplication. Most genetics textbooks, including Klug, et al. 2009, describe detailed mechanisms ...
Sporulation in BhciZZus subtilis 168. Control of
... temperature with a solution of p-nitrophenyl phosphate (15mg/ml in I -0M-diethanolamineHCI buffer, pH 10.0).Colonies with low alkaline phosphatase activity remained white, while normal colonies immediately turned a bright yellow. The white colonies were picked, streaked on glucose-glutamate minimal ...
... temperature with a solution of p-nitrophenyl phosphate (15mg/ml in I -0M-diethanolamineHCI buffer, pH 10.0).Colonies with low alkaline phosphatase activity remained white, while normal colonies immediately turned a bright yellow. The white colonies were picked, streaked on glucose-glutamate minimal ...
Article Positive and Purifying Selection on the Drosophila Y
... of genetic differentiation between the sex chromosomes and certainly contrasts with other systems such as mammals in which at least some sequence homology has been retained between the X and the Y over the length of the chromosomes (Lahn and Page 1999). Second, gene content of the Y chromosome appea ...
... of genetic differentiation between the sex chromosomes and certainly contrasts with other systems such as mammals in which at least some sequence homology has been retained between the X and the Y over the length of the chromosomes (Lahn and Page 1999). Second, gene content of the Y chromosome appea ...
Document
... Wednesday, January 28th Restate and answer on your openers page. 1. If quick growing fur (Q) is an incomplete dominant trait over slow growing fur (S). What type of trait would you expect an organism with the genotype QS to have? 2. Use a Punnett Square to figure out what percent chance two hybrid p ...
... Wednesday, January 28th Restate and answer on your openers page. 1. If quick growing fur (Q) is an incomplete dominant trait over slow growing fur (S). What type of trait would you expect an organism with the genotype QS to have? 2. Use a Punnett Square to figure out what percent chance two hybrid p ...
HOM-C/Hox genes and four interacting loci
... a support cell. The ends of the rays are located at reproducible positions in the cuticle, giving the male a characteristic, species-specific posterior morphology (Fig. 1D). In our previous studies we described the cellular mechanisms involved in the development of the adult ray pattern, and demonst ...
... a support cell. The ends of the rays are located at reproducible positions in the cuticle, giving the male a characteristic, species-specific posterior morphology (Fig. 1D). In our previous studies we described the cellular mechanisms involved in the development of the adult ray pattern, and demonst ...
Exclusion of known corneal dystrophy genes in an autosomal
... underlying causative genetic mechanism in a three-generation pedigree affected with a unique anterior membrane corneal dystrophy characterized by early onset recurrent corneal erosions, small discrete focal opacities at the level of Bowman layer and anterior stroma, anterior stromal flecks, and prom ...
... underlying causative genetic mechanism in a three-generation pedigree affected with a unique anterior membrane corneal dystrophy characterized by early onset recurrent corneal erosions, small discrete focal opacities at the level of Bowman layer and anterior stroma, anterior stromal flecks, and prom ...
Expression of the six chromate ion transporter
... digestions and by sequencing inserts using M13 forward/reverse universal primers. DNA fragments containing the chr genes were obtained by digestions with HindIII/XbaI or HindIII/EcoRI endonucleases and subcloned into the corresponding sites of pACYC184 or pUCP20 vectors. E. coli W3110 cells were tra ...
... digestions and by sequencing inserts using M13 forward/reverse universal primers. DNA fragments containing the chr genes were obtained by digestions with HindIII/XbaI or HindIII/EcoRI endonucleases and subcloned into the corresponding sites of pACYC184 or pUCP20 vectors. E. coli W3110 cells were tra ...
Agilent Whole Human Genome Oligo Microarray Kit
... Designed to truly represent the known genes in the human genome and their resulting transcripts, Agilent’s Whole Human Genome Oligo microarray is comprised of approximately 41,000 (60-mer) oligonucleotide probes, which span conserved exons across the transcripts of the targeted full-length genes. Th ...
... Designed to truly represent the known genes in the human genome and their resulting transcripts, Agilent’s Whole Human Genome Oligo microarray is comprised of approximately 41,000 (60-mer) oligonucleotide probes, which span conserved exons across the transcripts of the targeted full-length genes. Th ...
Oncogenomics
Oncogenomics is a relatively new sub-field of genomics that applies high throughput technologies to characterize genes associated with cancer. Oncogenomics is synonymous with ""cancer genomics"". Cancer is a genetic disease caused by accumulation of mutations to DNA leading to unrestrained cell proliferation and neoplasm formation. The goal of oncogenomics is to identify new oncogenes or tumor suppressor genes that may provide new insights into cancer diagnosis, predicting clinical outcome of cancers, and new targets for cancer therapies. The success of targeted cancer therapies such as Gleevec, Herceptin, and Avastin raised the hope for oncogenomics to elucidate new targets for cancer treatment.Besides understanding the underlying genetic mechanisms that initiates or drives cancer progression, one of the main goals of oncogenomics is to allow for the development of personalized cancer treatment. Cancer develops due to an accumulation of mutations in DNA. These mutations accumulate randomly, and thus, different DNA mutations and mutation combinations exist between different individuals with the same type of cancer. Thus, identifying and targeting specific mutations which have occurred in an individual patient may lead to increased efficacy of cancer therapy.The completion of the Human Genome Project has greatly facilitated the field of oncogenomics and has increased the abilities of researchers to find cancer causing genes. In addition, the sequencing technologies now available for sequence generation and data analysis have been applied to the study of oncogenomics. With the amount of research conducted on cancer genomes and the accumulation of databases documenting the mutational changes, it has been predicted that the most important cancer-causing mutations, rearrangements, and altered expression levels will be cataloged and well characterized within the next decade.Cancer research may look either on the genomic level at DNA mutations, the epigenetic level at methylation or histone modification changes, the transcription level at altered levels of gene expression, or the protein level at altered levels of protein abundance and function in cancer cells. Oncogenomics focuses on the genomic, epigenomic, and transcript level alterations in cancer.