Transgenic approaches for optogenetics Tim Murphy UBC.
... Cre activity can vary depending if transgene is inherited from the male or female parent. For example, EIIa-Cre, B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (003724), converts floxed alleles to complete knockout alleles because Cre is expressed throughout the mouse, but it is significantly more efficient when tr ...
... Cre activity can vary depending if transgene is inherited from the male or female parent. For example, EIIa-Cre, B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (003724), converts floxed alleles to complete knockout alleles because Cre is expressed throughout the mouse, but it is significantly more efficient when tr ...
Linkage, Recombination, and the Mapping of Genes on Chromosomes
... • Mitotic recombination: Rarely, recombination occurs during mitosis. In multicellular organisms, mitotic recombination can produce genetic mosaicism in which different cells have different genotypes. ...
... • Mitotic recombination: Rarely, recombination occurs during mitosis. In multicellular organisms, mitotic recombination can produce genetic mosaicism in which different cells have different genotypes. ...
Complete Characterization of the 3 Mouse Hereditary Hemochromatosis HFE Gene and
... FIG. 4. Splicing forms of HFE gene in the HepG2 cell line. The initially reported HFE cDNA (U60319) structure is represented at the top. Hatched boxes represent the coding HFE region. The ATG, the Stop codon and the polyadenylation site are indicated. Abbreviations inside the exons: L, peptide leade ...
... FIG. 4. Splicing forms of HFE gene in the HepG2 cell line. The initially reported HFE cDNA (U60319) structure is represented at the top. Hatched boxes represent the coding HFE region. The ATG, the Stop codon and the polyadenylation site are indicated. Abbreviations inside the exons: L, peptide leade ...
Article On the Complexity of Chloroplast RNA
... 2008), a possibility that we like to call the “spoiled kid hypothesis”. In this view, mutations accumulate over time in the chloroplast genome, compensated by suppressor mutations in the nucleus, driving an evolutionary ratchet. Such suppressors may be essential for optimal chloroplast function to e ...
... 2008), a possibility that we like to call the “spoiled kid hypothesis”. In this view, mutations accumulate over time in the chloroplast genome, compensated by suppressor mutations in the nucleus, driving an evolutionary ratchet. Such suppressors may be essential for optimal chloroplast function to e ...
Extrapolation to the whole human genome
... pseudogenes based on whether there is a continuous span of homology that is >70% of the length of the closest matching human protein (i.e. with introns removed), or whether there is evidence of polyadenylation. We have applied our approach to chromosomes 21 and 22, the first parts of the human genom ...
... pseudogenes based on whether there is a continuous span of homology that is >70% of the length of the closest matching human protein (i.e. with introns removed), or whether there is evidence of polyadenylation. We have applied our approach to chromosomes 21 and 22, the first parts of the human genom ...
Global Transcriptional Profiling Reveals Distinct Functions of Thymic
... regardless of age (Figure 1B), demonstrating that each stromal subset maintains its transcriptional identity during thymic involution. Stromal subsets at 6 months of age were further from their 1- and 3-month counterparts, indicative of age-related transcriptional changes in the data. Interestingly, ...
... regardless of age (Figure 1B), demonstrating that each stromal subset maintains its transcriptional identity during thymic involution. Stromal subsets at 6 months of age were further from their 1- and 3-month counterparts, indicative of age-related transcriptional changes in the data. Interestingly, ...
The photosynthetic apparatus of Prochlorococcus
... In open ocean ecosystems, carbon fixation is dominated by the closely related marine cyanobacteria Prochlorococcus and Synechococcus. Together they have been shown to contribute between 32 - 80% of the primary production in the oligotrophic oceans (Goericke and Welschmeyer, 1993; Li, 1995; Liu et al ...
... In open ocean ecosystems, carbon fixation is dominated by the closely related marine cyanobacteria Prochlorococcus and Synechococcus. Together they have been shown to contribute between 32 - 80% of the primary production in the oligotrophic oceans (Goericke and Welschmeyer, 1993; Li, 1995; Liu et al ...
Prader-Willi and Angelman syndromes: Sister imprinted disorders
... Read-through transcript Read-through transcript ...
... Read-through transcript Read-through transcript ...
PINK1- and Parkin- mediated mitophagy at a glance
... deletions in mitochondrial DNA (Bender et al., 2006; Kraytsberg et al., 2006) have also been identified in subtantia nigral neurons of PD patients. Although it is still not known why dopaminergic neurons appear to be more susceptible to mitochondrial damage than other tissues, one indication is that ...
... deletions in mitochondrial DNA (Bender et al., 2006; Kraytsberg et al., 2006) have also been identified in subtantia nigral neurons of PD patients. Although it is still not known why dopaminergic neurons appear to be more susceptible to mitochondrial damage than other tissues, one indication is that ...
The obstruction of adaptation in diploids by recessive deleterious
... to be much larger than that of the beneficial mutation (sb sd), and, for the final approximation, we further assume the beneficial mutation to be completely dominant and the deleterious mutation to be completely recessive (hb ≈ 1, hd ≈ 0). We emphasize that our model and analytic predictions can b ...
... to be much larger than that of the beneficial mutation (sb sd), and, for the final approximation, we further assume the beneficial mutation to be completely dominant and the deleterious mutation to be completely recessive (hb ≈ 1, hd ≈ 0). We emphasize that our model and analytic predictions can b ...
p53, the Cellular Gatekeeper Review for Growth and Division
... followed by a reduction to homozygosity. More rarely, deletions or chain-termination mutations in the p53 gene indicate that the null phenotype predisposes to cancer, as has been observed in mice with a homozygous p53 null mutation (Donehower et al., 1992). There have been some suggestions that the ...
... followed by a reduction to homozygosity. More rarely, deletions or chain-termination mutations in the p53 gene indicate that the null phenotype predisposes to cancer, as has been observed in mice with a homozygous p53 null mutation (Donehower et al., 1992). There have been some suggestions that the ...
Cloning of the Papaya Chromoplast-Specific
... Our group constructed a high-density genetic map of papaya using amplified fragment length polymorphism (AFLP) markers, and the flesh color locus was mapped near the end of linkage group 7 (LG7) and the two flanking markers were 3.4 and 3.7 centimorgan (cM), respectively (Ma et al., 2004). These clo ...
... Our group constructed a high-density genetic map of papaya using amplified fragment length polymorphism (AFLP) markers, and the flesh color locus was mapped near the end of linkage group 7 (LG7) and the two flanking markers were 3.4 and 3.7 centimorgan (cM), respectively (Ma et al., 2004). These clo ...
The genetic basis of inherited anomalies of the teeth: Part 1: Clinical
... epidermal growth factor (EGF), and the hedgehog (Hh) and wingless (Wnt) families [6]. In addition to these signals, the model proposed by Thesleff [7] also includes several genes, which are regulated by the signals in the responding tissues (Fig. 1). Mutations in many of these genes already have bee ...
... epidermal growth factor (EGF), and the hedgehog (Hh) and wingless (Wnt) families [6]. In addition to these signals, the model proposed by Thesleff [7] also includes several genes, which are regulated by the signals in the responding tissues (Fig. 1). Mutations in many of these genes already have bee ...
Review: Is genetic screening for hemochromatosis worthwhile?
... detecting subjects with delayed or incomplete penetrance, allowing diagnosis at an early age and treatment to prevent clinically significant iron overload [62]. However, not all subjects with iron overload carry the C282Y mutation. This mutation is mainly found in Caucasians. This limits the applicat ...
... detecting subjects with delayed or incomplete penetrance, allowing diagnosis at an early age and treatment to prevent clinically significant iron overload [62]. However, not all subjects with iron overload carry the C282Y mutation. This mutation is mainly found in Caucasians. This limits the applicat ...
PDF
... for hearing studies at Johns Hopkins University in Baltimore, MD, with the approval of their respective Internal Animal Care and Use Committees. Both colonies were established from random-bred, short- and long-haired cats obtained from commercial vendors and were maintained as closed colonies throug ...
... for hearing studies at Johns Hopkins University in Baltimore, MD, with the approval of their respective Internal Animal Care and Use Committees. Both colonies were established from random-bred, short- and long-haired cats obtained from commercial vendors and were maintained as closed colonies throug ...
ABSTRACT Using a bioinformatics approach to identify genes that
... The project herein describes a process that can be used to identify genes that have possible association with RP through comparing similar gene sets and identifying genes in those similar gene sets that are currently not associated with RP. These genes can then be further studied to determine their ...
... The project herein describes a process that can be used to identify genes that have possible association with RP through comparing similar gene sets and identifying genes in those similar gene sets that are currently not associated with RP. These genes can then be further studied to determine their ...
Category 2000
... with many functions, but is primarily involved in fat and energy metabolism. Therefore, it is a very interesting candidate gene for meat quality, which is one of the most important criteria in pig selection today. PPARGC1A however, does not have the same function in every tissue and there is still m ...
... with many functions, but is primarily involved in fat and energy metabolism. Therefore, it is a very interesting candidate gene for meat quality, which is one of the most important criteria in pig selection today. PPARGC1A however, does not have the same function in every tissue and there is still m ...
Archives of Microbiology
... Alternative nitrogenases are generally repressed by Mo, but expressed in the presence of tungsten even in Mo-medium (Raina et al. 1992; Thiel et al. 2002b). Remarkably, V-nitrogenases evolve three times more molecular hydrogen and have a higher energy demand than the Mo-enzyme (Eady 2003). At presen ...
... Alternative nitrogenases are generally repressed by Mo, but expressed in the presence of tungsten even in Mo-medium (Raina et al. 1992; Thiel et al. 2002b). Remarkably, V-nitrogenases evolve three times more molecular hydrogen and have a higher energy demand than the Mo-enzyme (Eady 2003). At presen ...
Oncogenomics
Oncogenomics is a relatively new sub-field of genomics that applies high throughput technologies to characterize genes associated with cancer. Oncogenomics is synonymous with ""cancer genomics"". Cancer is a genetic disease caused by accumulation of mutations to DNA leading to unrestrained cell proliferation and neoplasm formation. The goal of oncogenomics is to identify new oncogenes or tumor suppressor genes that may provide new insights into cancer diagnosis, predicting clinical outcome of cancers, and new targets for cancer therapies. The success of targeted cancer therapies such as Gleevec, Herceptin, and Avastin raised the hope for oncogenomics to elucidate new targets for cancer treatment.Besides understanding the underlying genetic mechanisms that initiates or drives cancer progression, one of the main goals of oncogenomics is to allow for the development of personalized cancer treatment. Cancer develops due to an accumulation of mutations in DNA. These mutations accumulate randomly, and thus, different DNA mutations and mutation combinations exist between different individuals with the same type of cancer. Thus, identifying and targeting specific mutations which have occurred in an individual patient may lead to increased efficacy of cancer therapy.The completion of the Human Genome Project has greatly facilitated the field of oncogenomics and has increased the abilities of researchers to find cancer causing genes. In addition, the sequencing technologies now available for sequence generation and data analysis have been applied to the study of oncogenomics. With the amount of research conducted on cancer genomes and the accumulation of databases documenting the mutational changes, it has been predicted that the most important cancer-causing mutations, rearrangements, and altered expression levels will be cataloged and well characterized within the next decade.Cancer research may look either on the genomic level at DNA mutations, the epigenetic level at methylation or histone modification changes, the transcription level at altered levels of gene expression, or the protein level at altered levels of protein abundance and function in cancer cells. Oncogenomics focuses on the genomic, epigenomic, and transcript level alterations in cancer.