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Role of Water Mediated Interactions in Protein
Role of Water Mediated Interactions in Protein

... of the search problem: only six rotational-translation degrees of freedom are lost during the association process. For instance, by constructing a grid in this space (given accurate interaction potentials), the protein docking problem can apparently be solved in a brute force manner. Starting from t ...
Chapter 3 Amino Acids, Peptides and Proteins
Chapter 3 Amino Acids, Peptides and Proteins

... • To monitor this reaction, we need to design an assay that allows us to observe the catalytic effect of our enzyme. • To develop an enzyme assay we must know the following: – The overall equation for the catalyzed reaction – An analytical procedure for determining the disappearance of substrate or ...
nutrient composition of dandelions and its potential as human food
nutrient composition of dandelions and its potential as human food

... walls. Normally herbivores assimilate the plant proteins and man consumes the herbivore protein and avoids the cellulose. This detour through the food chain is inefficient as only 2-20% of the plant protein fed to animals is recoverable as animal protein for human nutrition. The yield would be much ...
View/Open
View/Open

... Swine: Distiller’s dried grain by-products can be used as a protein/energy source in growing pigs and reproducing sows at limited inclusion rates. v DDGS contain approximately 27 percent crude protein, but are low in lysine and to a lesser extent threonine, leucine, isoleucine, and tryptophan v DDG ...
$doc.title

... The wondrous variety of colours displayed by flowers is due to four structurally distinct classes of pigments, namely flavonoids, carotenoids, betalains and chlorophyll. Chlorophyll is very rare as a flower pigment, and is only present in the handful of flowers that show a slight green hue (Eugster ...
sample written evaluation
sample written evaluation

... What are the results? Metabolic Costs of Amino Acid Biosynthesis Metabolic costs of amino acids are presented in table 1. The costs range from 11.7 to 74.3 and are calculated as: # of phosphate bonds + # of hydrogen atoms lost to the metabolic pathway by the diversion of the precursor metabolite. Fi ...
FC Chimeric Fusion Protein Purified Human
FC Chimeric Fusion Protein Purified Human

... (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissues and cells including the thymus, liver, ovary and lung. FasL is a member of the TNF cytokine family that induces apoptosis by binding to Fas, its cell-surface receptor ...
What is sequence alignment - department of computer & electrical
What is sequence alignment - department of computer & electrical

... If they are similar, then they might have similar structure and/or function. Identify conserved patterns (motifs) ...
Green Fluorescent Protein: A Reporter Molecule
Green Fluorescent Protein: A Reporter Molecule

... Study of biological processes (example: synthesis of proteins) ...
Homology between the DNA-binding domain of the GCN4
Homology between the DNA-binding domain of the GCN4

... thought to be mediated through a helix-turn-helix structure (13-16). Schemes for the prediction of secondary structure also reveal a strong preference for a-helical conformation in the carboxyl-terminal region of jun, which is homologous to the GCN4 DNA-binding domain. We suggest that the jun protei ...
Optimal Dietary Protein Levels in Juvenile Electric Blue Cichlid
Optimal Dietary Protein Levels in Juvenile Electric Blue Cichlid

... feeding trial, fish were fed to visual satiation and achieved maximum growth by controlling feed intake. In general, the feed intake dropped as the dietary protein level increased. This is in agreement with results for discus (Chong et al., 2000). Likewise, the FCR improved as the protein level incr ...
Protein Structure Prediction
Protein Structure Prediction

... The final network yields an overall prediction accuracy of 75.5% when tested by sevenfold cross-validation on a set of 426 non-homologous protein chains. The corresponding Qpred., Qobs. and MCC values are 49.8%, 72.3% and 0.43 respectively and are the best among all the previously published -turn p ...
Evolutionary predictions of binding surfaces and interactions Olivier
Evolutionary predictions of binding surfaces and interactions Olivier

... properties directly impact ligand interaction and transformation. Many important applications follow from their identification, including drug design, engineering protein mimetics and elucidating molecular pathways through site-directed mutagenesis [1,2••]. Furthermore, the proper identification of ...
search_2009
search_2009

... 3. Decrease the Expect value to 1.0 or lower  eliminates many hits and concentrate on results which are more likely to contain large coding regions and genomic fragments. 4. Processing multiple query sequences in one run can be much faster than processing them with separate runs because the databas ...
Is β-pleated sheet the molecular conformation which dictates
Is β-pleated sheet the molecular conformation which dictates

... (Willis, 1999) and are not included in our analysis. Rebers and Riddiford (1988) suggested that the consensus would turn out to be a region of structural importance. Subsequently, Andersen et al. (1995) suggested that the motif might be involved in protein/chitin interaction. The modified R&R Consen ...
Identification of Genes Potentially Regulated by Human
Identification of Genes Potentially Regulated by Human

... phenomena like senescence, growth-inhibition and mitochondrial dynamics, little is known about the specific network of genes that are involved in these processes or which might be dysregulated when hPNPaseold-35 is aberrantly expressed. Moreover, when evaluating its function as an exoribonuclease, w ...
Protein © 2009 Cengage - Wadsworth
Protein © 2009 Cengage - Wadsworth

... Protein turnover is the continual making and breaking down of protein.  Amino acid pool is the supply of amino acids that are available.  Amino acids from food are called ...
HiTrap Chelating HP 1 ml and 5 ml
HiTrap Chelating HP 1 ml and 5 ml

... excellent method for purifying recombinant proteins such as poly-histidine fusions, as well as many natural proteins. Metalloproteins are not usually suitable candidates for purification since they tend to scavenge the metal ions from the column. His-tagged protein purifications can be grouped into ...
Exploring Prostate Proteins and Antibodies
Exploring Prostate Proteins and Antibodies

... We have compiled a list of a selected number of antibodies against prostate specific proteins. Explore the links to the Human Protein Atlas in the table below, and see the tissue expression for the respective proteins in various tissues illustrated with RNA FPKM values as well as staining intensity ...
Evidence That Plant K+ Channel Proteins Have
Evidence That Plant K+ Channel Proteins Have

... A full-length cDNA encoding the bovine brain /3 subunit (KJ2) was recently cloned (Scott et al., 1994) and used to screen a rat brain cDNA library (Rettig et al., 1994). Two clones showing sequence homology were identified: rat K,Pl and rat K$2. One of the rat cDNAs, KvP2, encodes a deduced amino ac ...
Isolation of a New Member of the $100 Protein Family: Amino Acid
Isolation of a New Member of the $100 Protein Family: Amino Acid

... revealed that S100 is a member of a larger protein family that includes the cystic fibrosis antigen (6) also termed mrp-8 (44), the light chain of the cytoskeletal protein calpactin (8, 12, 18, 23), and predicted proteins derived from RNA abundantly expressed in fibroblasts (termed pEL98 [21], 18A2 ...
GFP-labelled Rubisco and aspartate aminotransferase are present
GFP-labelled Rubisco and aspartate aminotransferase are present

... amino acid linker peptide (GSGGG) was inserted between ASP5 and GFP to promote proper folding and activity of both proteins. The Rubisco small subunit construct consisted of the rbcS-3A coding region of P. sativum (Fluhr et al., 1986) followed by cyan ¯uorescent protein (mCFP) (Haseloff, 1999). This ...
Amino_Acids_and_Protein_Background_Info_
Amino_Acids_and_Protein_Background_Info_

... acids. Other animal and some plant proteins from corn, wheat, gelatin, soybeans, peanuts, potatoes, poultry, fish, and red meats in various combinations, can also provide the complete proteins that contain the amino acids essential to the body's health and well-being. (See PROTEIN IN FOODS in Resour ...
A Drosophila Third Chromosome Minute Locus Encodes
A Drosophila Third Chromosome Minute Locus Encodes

... M i n u t e s are non-additive in their phenotypic effect, i. e., the phenotype of a M I / + ; M 2 / + fly is not more extreme than the phenotypeof any ofthe single mutants. He concluded that the genes code for proteins with similar function (s) . The non-additive property of this type of mutations ...
Emerging real-time PCR applications.
Emerging real-time PCR applications.

... detect rare mutations, such as some somatic mutations, or mutations in shredded tumour cells in lymph or peripheral blood of cancer patients, or mutations in fetal cells present in peripheral blood of pregnant women. These targets are present in about one per 1,000-10,000 wild type sequences. An app ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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