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Design and Evolution of Artificial M13 Coat Proteins
Design and Evolution of Artificial M13 Coat Proteins

... phage particle, it resembles ACP-7 because it contains a cytoplasmic amino terminus with a positively-charged pattern similar to the carboxy terminus of P8 (Rapoza & Webster, 1995). The bacteriophage Pf3 major coat protein resembles M13 P8 both in its membrane-associated form and also as a component ...
GmPep914, an Eight-Amino Acid Peptide Isolated
GmPep914, an Eight-Amino Acid Peptide Isolated

... All of the above peptide signals were purified from crude plant leaf extracts based on their ability to interact with suspension cells, thereby causing a rapid increase in the extracellular pH of the media (Felix and Boller, 1995). An alkalinization assay was developed in the Ryan laboratory that ha ...
Hybrid Antibiotics
Hybrid Antibiotics

... increasing number of laboratories equipped with suitable methodology for their preparation. Biologically active secondary metabolites produced by microorganisms usually have complex structures so that their chemical synthesis is difficult and costly. The chance of derivatives of biologically active ...
A new subfamily of fungal subtilases: structural and functional
A new subfamily of fungal subtilases: structural and functional

... temperatures (Table 1). Amplification experiments for each pcsl ...
Alterations in gene expression in T1α null lung: a model of deficient
Alterations in gene expression in T1α null lung: a model of deficient

... their lungs with the first few breaths [11]. In normal late fetal and adult lungs, T1α protein is uniquely expressed in the apical membrane of type I alveolar epithelial cells, which form over 90% of the alveolar surface that is specialized for gas exchange [12-14]. In the absence of this protein th ...
Expression and purification of four different rhizobial acyl carrier
Expression and purification of four different rhizobial acyl carrier

... has been named AcpXL (Brozek et al., 1996). The overall amino acid sequence identities of these four ACPs range from only 26 to 32 % (Brozek et al., 1996), and a well-conserved amino acid region can only be observed around the phosphopantetheine-binding site. However, the three-dimensional structure ...
PROTEINS
PROTEINS

... This indicator is based on the assumption that more nitrogen is retained when the essential amino acids are present in sufficient amounts to meet the needs for growth. A food with a biological value of 70 or more is considered capable of supporting growth. The higher the biological value the better ...
Current Microbiology
Current Microbiology

... Indole-3-acetic acid (IAA), a major plant growth hormone of the auxin class, has been found to be produced by numerous organisms including of plants, bacteria, fungi, and algae. The identification of IAA intermediates demonstrated five different pathways of IAA biosynthesis in bacteria [15]. Many re ...
Document
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... Now, puromycin hydrochloride is particularly useful for the selection of cell types harbouring ...
Review the mechanism of protein folding
Review the mechanism of protein folding

... for expression and some may attached for some specialised folding and most destabilise polypeptide chain degraded by chaperones (Tomala, Korona ...
Transforming growth factor β1
Transforming growth factor β1

... properties and plays an important role in the regulation of cell proliferation and differentiation. These extraskeletal effects are relevant in the pathogenesis and treatment of many causes of chronic liver disease. (Lazo et al., 2011). Vitamin D mediates its intracellular signals via its receptor V ...
Positively selected sites on the surface glycoprotein (G) of infectious
Positively selected sites on the surface glycoprotein (G) of infectious

... et al., 2000; Troyer & Kurath, 2003) in the following respects: (i) all (except the outgroup) were descended from group M strains; (ii) the two major clades that were observed were divided so that clade A–B contained the reference sequences associated with subgroups A and B of group M as previously ...
Protein Metabolism and Acidosis
Protein Metabolism and Acidosis

... degradation between control and acidotic rats. This revealed that without ATP, acid induced proteolysis did not occur. Thus, it can be concluded that acidosis stimulates muscle protein degradation by activating the adenosine triphosphate-dependent pathway involving ubiquitin and proteasomes. (Figure ...
The Logic Linking Protein Acetylation and Metabolism
The Logic Linking Protein Acetylation and Metabolism

... CDKs to drive the cell cycle under these conditions. More generally, phosphorylation-based signaling pathways may impinge on sirtuins to exert an additional layer of control over protein acetylation. How will the flow of carbon provide acetyl-CoA for acetylation of cytosolic/ nuclear proteins under ...
Statistical analysis of DNA microarray data
Statistical analysis of DNA microarray data

... describe the entire complement of proteins in a given biological organism or system at a given time, i.e. the protein products of the genome. The term has been applied to several different types of biological systems. A cellular proteome is the collection of proteins found in a particular cell type ...
Slide 1
Slide 1

... - Approximately 25-40% above basal metabolic needs as measured by metabolic cart or estimated with equations (de Klerk et al, Int Care Med, 2002). -20 to 25% as carbohydrates (insulin as needed) 30 to 40% lipid formulations (20% lipid emulsions) 40 to 50% protein ...
Characterizing the Secreted Proteome of Mycobacterium
Characterizing the Secreted Proteome of Mycobacterium

... Key Features for Detecting and Quantifying Secreted Proteins The goal of this research was to characterize proteins secreted specifically by the ESX-1 secretion apparatus into the culture supernatant. The analytical challenge lies in the fact that there are many other proteins present in the culture ...
Psittacine Beak and Feather Disease Virus Nucleotide Sequence
Psittacine Beak and Feather Disease Virus Nucleotide Sequence

... features of the genome were most closely related to PCV. Like PCV (Meehan et al., 1997), BFDV contained seven major ORFs and lacked a distinctive noncoding region, thus affording highly efficient use of genetic material in both of these viruses. Both viruses have three ORFs in the encapsidated stran ...
Article - Nature
Article - Nature

... GrhP,13 RubR, ORF11,14 and TblS, which belong to the same clade, have no catalytic Cys ...
Complete sequence analysis of the genome of the bacterium
Complete sequence analysis of the genome of the bacterium

... The central enzyme for DNA replication in bacteria is the DNA polymerase III holoenzyme (32), which consists of 10 subunits in E.coli, a DNA polymerase subunit α and nine accessory proteins (ε, υ, τ, γ, δ, δ′, χ, ψ and β). Mycoplasma pneumoniae codes for two potential α subunits (the gene name in th ...
attachment 2 - Food Standards Australia New Zealand
attachment 2 - Food Standards Australia New Zealand

... including the phenoxy auxins such as 2,4-dichlorophenoxyacetic acid (2,4-D), and to the herbicides glufosinate ammonium and glyphosate. Tolerance to 2,4-D is achieved through expression of the enzyme aryloxyalkanoatedioxygenase-12 (AAD-12) encoded by the aad-12 gene derived from Delftia acidovorans, ...
PURIFICATION OF TAP TAGGED YEAST PROTEINS  Annika Väntänen
PURIFICATION OF TAP TAGGED YEAST PROTEINS Annika Väntänen

... for example 3-hydroxymyristoyl-ACP, are possibly used for protein acylation which is one type of lipid modifications of proteins. The best characterised physiological function of the pathway is to provide the octanoyl chain (octanoylACP), a substrate, for lipoic acid synthesis which seems to be an e ...
SOD binds cell-adhesive peroxidase - Journal of Cell Science
SOD binds cell-adhesive peroxidase - Journal of Cell Science

... surface; it could be released from the membrane with high salt. It was thus concluded that the peroxinectin-binding protein is an extracellular SOD (EC-SOD) and a peripheral membrane protein, presumably kept at the cell surface via ionic interaction with its C-terminal region. This interaction with ...
The relative molecular mass, heterogeneity and subunit composition
The relative molecular mass, heterogeneity and subunit composition

... (Fig. Ib), yielded a value of 280000 f 30000, which confirms the result of Schwenke et al. (1980), who used a less direct procedure (combining sedimentation & diffusion coefficients via the Svedberg equation). It differs significantly from the value obtained by Gill & Tung (1976), who also used a se ...
Presentation - people.vcu.edu
Presentation - people.vcu.edu

... • Does MeCP2 specifically affect MBP and MAG? ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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