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SeeProtStructFromPDB
SeeProtStructFromPDB

... This tutorial serves as a basic introduction to viewing protein structures in Protein Data Bank (PDB), an international archive of structural data for biological molecules. In addition to summary information for a particular molecular structure, PDB also provides different viewing options. A structu ...
Systemic Organ Wasting Induced by Localized Expression of the
Systemic Organ Wasting Induced by Localized Expression of the

... 2004). Downregulation of IGF-1 signaling in skeletal muscles decreases Akt activity and in turn increases Foxo activity, which induces muscle protein degradation through the ubiquitin-proteasome system and autophagy (Han et al., 2013). Moreover, the transforming growth factor b family members myosta ...
Folie 1 - FLI
Folie 1 - FLI

... Chou-Fasman Secondary Structure Prediction • Assign all of the residues in the peptide the appropriate set of parameters. • Scan through the peptide and identify regions where 4 out of 6 contiguous residues have P(a-helix) > 100. • That region is declared an alpha-helix. Extend the helix in both di ...
PDF - FEMS Microbiology Letters
PDF - FEMS Microbiology Letters

... The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 58, and cloned into pIN-III(lppp-5)A3. The resulting plasmid p ...
Innovative Purification Protocol for Heparin Binding
Innovative Purification Protocol for Heparin Binding

... controlled heating for milk, beer, etc, without affecting flavors [39-41]. The advances in the science of biology in the 18th and 19th centuries allowed the development of microbial bases for the brewing and baking process [42]. The post second world war period saw the application of pharmaceutical ...
Expression of a novel cadherin (EP-cadherin) in unfertilized eggs
Expression of a novel cadherin (EP-cadherin) in unfertilized eggs

... The differences found between the two are marked in Fig. 2. The nucleotide and the deduced amino acid sequences of clone 4 are shown in Fig. 3. Comparison of the protein sequence to that of known cadherins revealed considerable degree of homology to both Eand P-cadherin is shown in Fig. 4. As can be ...
Atom depth in protein structure and function
Atom depth in protein structure and function

... area. Depth has been found to be correlated with several molecular, residue and atomic properties, such as average protein domain size, protein stability, free energy of formation of protein complexes, amino acid type hydrophobicity, residue conservation and hydrogen/deuterium amide proton exchange ...
IOSR Journal of Computer Engineering (IOSR-JCE)
IOSR Journal of Computer Engineering (IOSR-JCE)

... repetitions, direct repetitions, palindrome repetitions, adjourning repetitions. At first, the protein sequences are processed and repetitions are detected. Then the repetitions are parsed using SCP and two outputs can be obtained such as (1) Dictionary items, D which contains repeated patterns, rep ...
An hierarchical artificial neural network system for the classification
An hierarchical artificial neural network system for the classification

... However, these algorithms focus on the localization of transmembrane segments in known integral membrane proteins and are not suited to the discrimination of membrane proteins from non-membrane proteins. Recently, we have published the PRED-TMR method in an attempt to improve the fine localization o ...
The protein acetylome and the regulation of metabolism - Serval
The protein acetylome and the regulation of metabolism - Serval

... (Nat3) and Mitochondrial Distribution and Morphology 20 (Mdm20), while the N-Acetyl Transferase C complex (NatC) contains the Maintenance of Killer proteins Mak3, Mak10 and Mak31. Homologs to all these subunits are present in plants, and complementation of yeast natc mutants with the homologous Arab ...
The Role of the C-terminal Tail of the Ribosomal Protein S13 in Pr
The Role of the C-terminal Tail of the Ribosomal Protein S13 in Pr

... ME6)  encodes  a  hexa‐h histidine  afffinity  tag  att  2008.  This  strain  (derived  from  the  p  the  3’‐end  of  the  rpllL  gene  as  well  as  a  kanamycin  resistance  gene;  thus  enabling  single  step for  researrch  as  it  enables  bacterial  ribosome  purification  which  is  convenie ...
The Three Dimensional Structure of Proteins
The Three Dimensional Structure of Proteins

... Binding Domain (or the Rossman Fold), the Sushi Domain plays a role in protein-protein binding, the Kringle Domain often plays a role in protein-ligand and/or protein-protein binding, and the Epidermal Growth Factor Domain may be / is involved in interactions between extracellular proteins and the c ...
PDF - World Wide Journals
PDF - World Wide Journals

... feathers is used in other industrial applications such as clothing, insulation and bedding, producing biodegradable polymers and enzymes and also as a medium for culturing microbes. A higher quantity of pretreated feather is utilized to produce a digestible dietary protein feedstuff for poultry and ...
Chapter 5 - macromolecules
Chapter 5 - macromolecules

... bonds that form between the —OH group on the 3 carbon of one nucleotide and the phosphate on the 5 carbon on the next abc…lmnop • sugar-phosphate backbone (side of ladder) with nitrogenous bases as rungs of ladder • The sequence of bases along a DNA or mRNA polymer is the genetic code, the instruc ...
$doc.title

... be established, using a combination of enzymes found in various diverse, unrelated organisms. These novel pigmentation pathways can then lead to the expression of blue or black pigments that have never before been found in any flower. ...
LIN-28 co-transcriptionally binds primary let
LIN-28 co-transcriptionally binds primary let

... MicroRNAs (miRNAs) function as ~22-nucleotide (nt) guide RNAs in the RNA-induced silencing complex (RISC) by binding to partially complementary sites in target mRNAs, causing inhibition of translation or destabilization. Let-7 miRNA that originally discovered in C. elegans is conserved across specie ...
Decision Document
Decision Document

... On October 22nd, 2004, an application was received from Monsanto S.A.I.C., to carry out the assessment of human and animal food safety of the transformation event LY038 x MON810, high lysine corn with Lepidoptera insects resistance. This application was reviewed in order to confirm the compliance wi ...
Biosynthesis of the dystonia-associated AAA ATPase torsinA at the
Biosynthesis of the dystonia-associated AAA ATPase torsinA at the

... present in mammalian cells [4,5,10,11]. In order to characterize these, we first examined the biosynthesis of torsinA in a cell-free translation system. Four distinct membrane-associated products were generated when translation was carried out in the presence of ER-derived membranes (Figure 1B, lane ...
A Support Vector Machine Approach for LTP Using Amino Acid
A Support Vector Machine Approach for LTP Using Amino Acid

... newly developed method was tested with the above six plants. Comparison with All-plant method In this test, an All-plant method was developed in SVM using all the three kernels. For creating this, six plants were taken namely arabidopsis (Arabidopsis thaliana), wheat (Triticum aestivum), maize (Zea ...
Full-Text
Full-Text

... nitrogen sources. Other yeast prions are involved in the regulation of transcription, translation and in the biogenesis of ribosomes [28]. Even though amyloids in yeast do not cause severe disease as in mammals, they share several features with prions as they are transmissible and continuously repli ...
Genetic and biochemical identification of the
Genetic and biochemical identification of the

... Taq DNA polymerase (Transgen). PCR products were purified using an agarose gel DNA fragment recovery kit (TIANGEN). Cloning of PCR fragments was performed with the pEASY-T1 cloning vector systems (Transgen). Various plasmids (Table 1) for genetic disruption and complementation in C. glutamicum and f ...
Local protein synthesis in neuronal axons: why and
Local protein synthesis in neuronal axons: why and

... a series of important discoveries that have firmly established local protein synthesis as one of key mechanisms underlying activity-dependent structural changes in the synapse (3). In contrast to the dendrite, however, polyribosomes were not readily detected on the other side of the synapse, the axo ...
DNA Transformation LAb
DNA Transformation LAb

...  Cells that can be transformed by DNA  Some can become competent by certain environmental conditions  EX. E-coli ...
Genetically Encoded Fluorescent Reporters of Histone
Genetically Encoded Fluorescent Reporters of Histone

... proteins around which DNA is wrapped in chromatin. Phosphorylation, acetylation, and methylation of the N-terminal tails of histone proteins strongly influence transcription of neighboring genes by altering steric access to DNA and by introducing or removing protein docking sites.1 Despite the impor ...
Heat shock response in hyperthermophilic microorganisms
Heat shock response in hyperthermophilic microorganisms

... constitutively and is the major protein produced during heat shock. Its constitutive expression and high abundance during normal growth suggests that the chaperone is important for stabilizing protein intermediates at all growth temperatures. Hyperthermophilic archaea are unique in that they do not ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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