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Heat shock response in hyperthermophilic microorganisms
Heat shock response in hyperthermophilic microorganisms

... constitutively and is the major protein produced during heat shock. Its constitutive expression and high abundance during normal growth suggests that the chaperone is important for stabilizing protein intermediates at all growth temperatures. Hyperthermophilic archaea are unique in that they do not ...
The Carboxyl-Terminal Region of Protein C Is
The Carboxyl-Terminal Region of Protein C Is

... (Fig 4e). Mock-transfected CHO cells did not show any specific immunolabelling (Fig 4g and h). From these results, we conclude that the transport of PC407 and PC408 from rough ER to the Golgi apparatus is blocked and that these mutant proteins are retained in the rough ER, whereas PC409 was transpor ...
sorghum protein - Simply Sorghum
sorghum protein - Simply Sorghum

... bran is a rich source of condensed tannins, also known as proanthocyanidins, specifically containing epicatechin and catechin. Black sorghum bran contains anthocyanins, including 3-deoxyanthocyanins (apigeninidin) and luteolinidin7. The high levels of 3-deoxyanthocyanins are relatively rare in natur ...


... h) stop codon i) mRNA termination ii) Discuss the role of any 4 of the 9 labels in the expression of recombinant protein. Be sure to indicate your choices. a) Antibiotic resistance gene: Provides a method to insure the plasmid remains in the bacteria. Only those bacteria with the plasmid will grow i ...
the cortex adhaerens mosaic of lens fiber cells
the cortex adhaerens mosaic of lens fiber cells

... cadherin-based, catenin-containing complexes, alternating with patches formed by the EPPD complexes. The latter, a new type of junctional plaque ensemble of proteins hitherto known only from certain other cell types, must be added to the list of major lens cortex proteins. We here discuss its possib ...
Measurement of the synthesis of liver
Measurement of the synthesis of liver

... It is important to keep these limitations in mind in the consideration of all in vitro work, as it may be argued that optimum function is not being achieved under the conditions of study. It seems reasonable, none the less, to use such systems for comparative studies, especially where control period ...
Proteins - Cathkin High School
Proteins - Cathkin High School

... acids known as essential amino acids. Only eight amino acids are regarded as being essential for humans although a further two are required in childhood. Some amino acids have more than one amino group or more than one carboxyl group and these allow chains to form branches. With twenty different ami ...
locating domains
locating domains

... • Site-directed mutagenesis studies, which can give insights as to residues involved in active or binding sites • Knowledge of proteolytic cleavage sites, post-translational modifications, such as phosphorylation or glycosylation can suggest residues that must be accessible, etc. • Remember to keep ...
The 1B (NS2), 1C (NS1) and N Proteins of Human Respiratory
The 1B (NS2), 1C (NS1) and N Proteins of Human Respiratory

... where the Long strain differed from A2. At these 15 positions, none of the nucleotide differences in any strain resulted in a change in amino acid coding assignment. For these 15 positions, strains A2 and 18537 were identical at five nucleotides, Long and 18537 were identical at nine, and 18537 was ...
PBI 6 Features on Teacher`s Map 2-08.qxp
PBI 6 Features on Teacher`s Map 2-08.qxp

... The Translation Start Site (AUG) is located at nucleotides 62,187- 62,189. All proteins begin with the amino acid methionine, Met, encoded by nucleotides AUG. This rule is a consequence of the mechanism that cells use to begin protein synthesis; a special Met-tRNA initiates assembly of the two subun ...
Cloning of Murine gp91ph”” cDNA and Functional
Cloning of Murine gp91ph”” cDNA and Functional

... kD that was immunoreactive with the gp9IPh"'antibody and which comigrated with the gp91ph"' species detected in nonadherent murine BM cells (of which -50% are neutrophils and -30% mononuclear phagocytes) (Fig 2A, lanes 7 and 8). There was some clone-to-clone variation in the level of expression, whi ...
Post Exercise Carbohydrates May Be Counter
Post Exercise Carbohydrates May Be Counter

... At this time the consensus in the literature is that the use of a balanced amino acid mixture along with glucose or high glycemic carbohydrates taken immediately after exercise and then again a short time later would seem to optimize the immediate anabolic effects of exercise. 1 There’s no doubt tha ...
Rare variant discovery using family based studies / John Blangero
Rare variant discovery using family based studies / John Blangero

... •  SNP  located  within  exon  1  of  MOG   •  A  membrane  protein  expressed  on   the  oligodendrocyte  cell  surface   and  the  outermost  surface  of   myelin  sheaths   •  Primary  target  an
The nucleotide sequence of the gene encoding the attachment
The nucleotide sequence of the gene encoding the attachment

... the L gene of CDV (see Fig. 1). The numbering in this sequence begins at the conserved gene start sequence in the H gene and ends at position 1946 of the consensus 3'terminal sequence. Genes of similar size have been described for MV (1954 nucleotides; Alkhatib & Briedis, 1986) and RPV (1953 nucleot ...
Functional second genes generated by retrotransposition of the X
Functional second genes generated by retrotransposition of the X

... marrow (13,14). So far, this is the only reported case in which an RP gene mutation has been found associated with human disease, although how this RP defect causes the DBA phenotype is still unknown. Together with the above-mentioned Drosophila mutants, this implies that two copies of each RP gene ...
Protein Structure Analysis
Protein Structure Analysis

... Experimental electron density map and model fitting (apoE four helix bundle) ...
Protein Structure
Protein Structure

... The charged amino acids may be either acidic or basic. At low pH, proteins are positively charged due to the basic groups on lysine and arginine, whereas, at high pH, proteins are negatively charged due to the acidic groups on aspartic and glutamic acids. The peptide backbone of proteins is composed ...
Invention
Invention

... modulating the activity of IRDBP-1 by increasing at least one of the intracellular level, DNA binding activity, gene transcriptional activity, proteolytic cleavage, nuclear entry, and phosphorylation of IRDBP-1, thereby increasing glucose transport into a cell of the animal or human, wherein when th ...
What is metabolic engineering?
What is metabolic engineering?

... • The basic construction of such protein chips has some similarities to DNA chips, such as the use of a glass or plastic surface dotted with an array of molecules. • Known proteins are analyzed using functional assays that are on the chip. For example, chip surfaces can contain enzymes, receptor pro ...
Block III - Madhya Pradesh Bhoj Open University
Block III - Madhya Pradesh Bhoj Open University

... bacteria. Partial destruction of cell wall takes place at the point of contact. At this site, Rhizobium embedded in a thread of mucilagineous substance invades in the root tissue and multiplies within the root hair. Some of the bacteria enlarge to form membrane bound structures called bacteriods. Th ...
Protein Structure Analysis - G
Protein Structure Analysis - G

... A β-sheet is formed when hydrogen bonds are formed between two parts of the protein chain that can be far apart. The Tertiary structure is basically the folding of the α-helices and β-sheets into a more complex structure by the interaction of their amino acid side chains. The rules governing the dif ...
Bax - Hypromatrix
Bax - Hypromatrix

... 0.2 µg/µl, store at 4 oC For research use only BACKGROUND Bax (for Bcl-associated X protein) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax has extensive ...
Genes required for Lactococcus garvieae survival in a fish host
Genes required for Lactococcus garvieae survival in a fish host

... Laboratory media appear to be a limited tool when trying to study the molecular mechanisms of disease, since it is very difficult to mimic the complex and changing environment of the host. In an attempt to overcome these limitations, Hensel et al. (1995) developed a method for ...
File
File

... • Nucleic acids are polymers of nucleotides that store and transmit genetic information. – Deoxyribonucleic acid (DNA) holds the genetic information in all cellular organisms and some ...
Amino Acids - CSUN Moodle
Amino Acids - CSUN Moodle

... The Human Genome Project • Sequencing of the human genome allows for: – Identification and categorization of different haplotypes – Understanding the differences between humans and chimpanzees • Based on phylogenetic trees and comparison of differences • Especially in regulatory sequences, which ma ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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