Sugar Transport in (Hyper-)Thermophilic Archaea
... tengcongensis (Bao et al., 2002) contain a number of genes that are uniquely homologous to archaeal genes. It was proposed that due to adaptation to hyperthermophily, lateral gene transfer exists between hyperthermophilic bacteria and archaea (Nesbo et al., 2001). The highest maximal growth temperat ...
... tengcongensis (Bao et al., 2002) contain a number of genes that are uniquely homologous to archaeal genes. It was proposed that due to adaptation to hyperthermophily, lateral gene transfer exists between hyperthermophilic bacteria and archaea (Nesbo et al., 2001). The highest maximal growth temperat ...
Protein synthesis: Twenty three amino acids and
... Another key point in this approach was the use of tyrosyltRNA synthetase, which does not have any known editing activity. The principles of this approach are not specific to a particular amino acid, and so can be extended to other synthetase–tRNA pairs on a case-by-case basis for the selection (from ...
... Another key point in this approach was the use of tyrosyltRNA synthetase, which does not have any known editing activity. The principles of this approach are not specific to a particular amino acid, and so can be extended to other synthetase–tRNA pairs on a case-by-case basis for the selection (from ...
Effects of glucose and insulin on insulin receptor gene expression
... nucleotide probe of region -57 to -35 of the FAS gene were linked to a reporter gene and the resultant construct was used for transfection, the reporter gene activity was significantly increased in hepatocytes in response to glucose/insulin treatment and the glucose/insulin stimulation was suppresse ...
... nucleotide probe of region -57 to -35 of the FAS gene were linked to a reporter gene and the resultant construct was used for transfection, the reporter gene activity was significantly increased in hepatocytes in response to glucose/insulin treatment and the glucose/insulin stimulation was suppresse ...
Anti-c-myc antibody 9E10 - Protein Engineering, Design and Selection
... Fig. 2. Western blot of purified scFv 1F9 variants with myc-tag fusions. Shortened (snmyc) and shortened and mutated (mutmyc) myc-tags are compared to sc-Fv containing the original myc-tag sequence (EQKLISEEDLN). The scFv variants with the changed myc-tags have as short a tag as possible, the last t ...
... Fig. 2. Western blot of purified scFv 1F9 variants with myc-tag fusions. Shortened (snmyc) and shortened and mutated (mutmyc) myc-tags are compared to sc-Fv containing the original myc-tag sequence (EQKLISEEDLN). The scFv variants with the changed myc-tags have as short a tag as possible, the last t ...
The common northern periwinkle, Littorina littorea
... values within hours (Fig. 2). Complementing these findings, L. littorea hepatopancreas also showed a dramatic reduction in nuclear transcription rates during anoxia. The rate of mRNA elongation (measured as 32P-UTP incorporation into nascent mRNAs by isolated nuclei) dropped to less than one-third o ...
... values within hours (Fig. 2). Complementing these findings, L. littorea hepatopancreas also showed a dramatic reduction in nuclear transcription rates during anoxia. The rate of mRNA elongation (measured as 32P-UTP incorporation into nascent mRNAs by isolated nuclei) dropped to less than one-third o ...
A prophage-encoded actin-like protein required for efficient viral
... The relative amount of circular phage DNA was determined via quantitative PCR (qPCR). Therefore, C. glutamicum wild type, the alpC deletion strain, and the alpA deletion strain were grown in 5 ml BHI (Brain Heart Infusion, Difco) for about 6 h at 30◦ C. A second precultivation was performed in CGXII ...
... The relative amount of circular phage DNA was determined via quantitative PCR (qPCR). Therefore, C. glutamicum wild type, the alpC deletion strain, and the alpA deletion strain were grown in 5 ml BHI (Brain Heart Infusion, Difco) for about 6 h at 30◦ C. A second precultivation was performed in CGXII ...
Amino Acid Metabolism
... enzymes, and muscle tissue. The primary function of protein is growth and repair of body tissue (anabolism). Proteins can also be used as energy through catabolic (breakdown of tissues) reactions, such as gluconeogenesis—the process of making glucose from amino acids, lactate, glycerol, or pyruvate ...
... enzymes, and muscle tissue. The primary function of protein is growth and repair of body tissue (anabolism). Proteins can also be used as energy through catabolic (breakdown of tissues) reactions, such as gluconeogenesis—the process of making glucose from amino acids, lactate, glycerol, or pyruvate ...
On the trail of protein sequences
... My introduction to computers came about from an interest in biochemical evolution, a subject that first fascinated me many years ago when I was a graduate student. The laboratory in which I did my graduate training was working on blood proteins—especially those involved in blood coagulation—and a nu ...
... My introduction to computers came about from an interest in biochemical evolution, a subject that first fascinated me many years ago when I was a graduate student. The laboratory in which I did my graduate training was working on blood proteins—especially those involved in blood coagulation—and a nu ...
Exploring your protein - QIAGEN Bioinformatics
... Figure 16: The CRAC motif in Prosite format. It specifies that going from the N- to the C-terminus the first amino acid should be a leucine or a valine, then one to five amino acids of any kind, then a tyrosine, then again one to five amino acids of any kind, and finally a lysine or an arginine. Two ...
... Figure 16: The CRAC motif in Prosite format. It specifies that going from the N- to the C-terminus the first amino acid should be a leucine or a valine, then one to five amino acids of any kind, then a tyrosine, then again one to five amino acids of any kind, and finally a lysine or an arginine. Two ...
Directed Evolution of ATP Binding Proteins from a Zinc Finger
... RXR117 and RXR122 were discovered from a subsequent screen of post-round 8 sequences (see Supplemental Data) for soluble proteins by using fusions to green fluorescent protein (GFP) in E. coli [35]. In order to obtain concentrated protein samples for biochemical and biophysical characterization, the ...
... RXR117 and RXR122 were discovered from a subsequent screen of post-round 8 sequences (see Supplemental Data) for soluble proteins by using fusions to green fluorescent protein (GFP) in E. coli [35]. In order to obtain concentrated protein samples for biochemical and biophysical characterization, the ...
A Protein Factor in the Nutrition of Paramecium
... linoleic and oleic acid. After some preliminhry work with natural oils, particularly corn oil, it was found that these two fatty acids in a three to one ratio promoted the best growth of Pararnecium caudatum. The fact that in the early work with all three species the yeast autolysates contained both ...
... linoleic and oleic acid. After some preliminhry work with natural oils, particularly corn oil, it was found that these two fatty acids in a three to one ratio promoted the best growth of Pararnecium caudatum. The fact that in the early work with all three species the yeast autolysates contained both ...
Simple Models of Protein Folding
... amino acids, namely hydrophobic and hydrophilic each with a spin value 0 or 1. The energy between non-sequential residues is then determined based on both the spin and type of residue [2]. One of the main qualitative differences presented by the increased complexity of the four amino acid type model ...
... amino acids, namely hydrophobic and hydrophilic each with a spin value 0 or 1. The energy between non-sequential residues is then determined based on both the spin and type of residue [2]. One of the main qualitative differences presented by the increased complexity of the four amino acid type model ...
Amino Acid Requirements and Post-absorptive Metabolism in Cattle
... The largest component of the so-called maintenance requirement is MFP. As discussed previously, this component currently differs substantially between NRC (2001) and CNCPS (Fox et al., 2004, Table 1). Another peculiarity of this component of requirement is that no efficiency of utilization of absorb ...
... The largest component of the so-called maintenance requirement is MFP. As discussed previously, this component currently differs substantially between NRC (2001) and CNCPS (Fox et al., 2004, Table 1). Another peculiarity of this component of requirement is that no efficiency of utilization of absorb ...
Identification of a family of BspA like surface proteins of Entamoeba
... weight of the EhLRRP1 protein is 60.5 kDa, and the larger size of the peptide recognized in both reducing and non-reducing gels (data not shown) likely represents post-translational modifications to the EhLRRP1 protein. The EhLRRP1 protein has multiple potential phosphorylation sites and one glycosy ...
... weight of the EhLRRP1 protein is 60.5 kDa, and the larger size of the peptide recognized in both reducing and non-reducing gels (data not shown) likely represents post-translational modifications to the EhLRRP1 protein. The EhLRRP1 protein has multiple potential phosphorylation sites and one glycosy ...
Stringent Response in Myxococcus xanthus
... How do we test to see if DK527is really a relA- mutant? ...
... How do we test to see if DK527is really a relA- mutant? ...
AminoMax Updates.
... If sample 3 is compared to Sample 1, there is still an improvement in digestible RUP. However, the indigestible material, which will be lost in manure, also increased. Thus, it is not really meaningful to compare ingredients based on RUP. It is important to be aware of the indigestible fraction as w ...
... If sample 3 is compared to Sample 1, there is still an improvement in digestible RUP. However, the indigestible material, which will be lost in manure, also increased. Thus, it is not really meaningful to compare ingredients based on RUP. It is important to be aware of the indigestible fraction as w ...
Nucleotide sequence of the thioredoxin gene from
... the sequence of another Sau3A fragment (Fig. 2) from the thioredoxin gene~ a 15-mer oligodeoxyribonucleotide was synthesized (Table 1) for use as a primer in the sequence analysis. The isolated 3-kb fragment was used as template in direct dideoxy sequence analysis after heat-induced strand separatio ...
... the sequence of another Sau3A fragment (Fig. 2) from the thioredoxin gene~ a 15-mer oligodeoxyribonucleotide was synthesized (Table 1) for use as a primer in the sequence analysis. The isolated 3-kb fragment was used as template in direct dideoxy sequence analysis after heat-induced strand separatio ...
Polyunsaturated fatty acids stimulate hepatic UCP
... insulin, 1 U/ml penicillin, 1 g/ml streptomycin, 2 mM Lglutamine, and 5.5 mM glucose before experimental treatment. After the plating incubation, 667 g of Matrigel (Collaborative Biomedical Products, Bedford, MA) were added to each plate to provide an extracellular matrix overlay (33). Determinati ...
... insulin, 1 U/ml penicillin, 1 g/ml streptomycin, 2 mM Lglutamine, and 5.5 mM glucose before experimental treatment. After the plating incubation, 667 g of Matrigel (Collaborative Biomedical Products, Bedford, MA) were added to each plate to provide an extracellular matrix overlay (33). Determinati ...
Detailed proteome analysis of growing cells of the planctomycete
... families (Fig. 1, Table 1). Other functional protein groups being highly abundant in the intracellular proteome are involved in carbohydrate metabolism (particular glycolysis and the pentose phosphate pathway) and transport processes. The results indicate that, although R. baltica is a slowly growin ...
... families (Fig. 1, Table 1). Other functional protein groups being highly abundant in the intracellular proteome are involved in carbohydrate metabolism (particular glycolysis and the pentose phosphate pathway) and transport processes. The results indicate that, although R. baltica is a slowly growin ...
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... The reaction can be halted by addition of acid or another stop reagent. Using sulfuric acid turns TMB yellow. The colour may be read at 450 nm. ...
... The reaction can be halted by addition of acid or another stop reagent. Using sulfuric acid turns TMB yellow. The colour may be read at 450 nm. ...
amino acid
... tubulin, and а variety of other proteins comprise the cytoskeleton. Defense. А wide variety of proteins have а protective role. Examples found in vertebrates include keratin, the protein found in skin cells that aids in protecting the organism against mechanical and chemical injury. The blood-clotti ...
... tubulin, and а variety of other proteins comprise the cytoskeleton. Defense. А wide variety of proteins have а protective role. Examples found in vertebrates include keratin, the protein found in skin cells that aids in protecting the organism against mechanical and chemical injury. The blood-clotti ...
BioPerf: A Benchmark Suite to Evaluate High-Performance
... has spurred growing interest in large scale analysis of genetic information. Bioinformatics applications, which explore computational methods to allow researchers to sift through the massive biological data and extract useful information, are becoming increasingly important computer workloads. This ...
... has spurred growing interest in large scale analysis of genetic information. Bioinformatics applications, which explore computational methods to allow researchers to sift through the massive biological data and extract useful information, are becoming increasingly important computer workloads. This ...
Powerpoint
... Protein Kinase-like Superfamily E. Scheeff and P.E. Bourne 2005 PLoS Comp. Biol. 1(5): e49. ...
... Protein Kinase-like Superfamily E. Scheeff and P.E. Bourne 2005 PLoS Comp. Biol. 1(5): e49. ...
A novel atlas of gene expression in human skeletal
... metabolic link between mTOR and glycolysis has also been described where low glycolytic flux leads to binding of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to the mTORC1-regulator Rheb thereby inhibiting mTORC1 signaling and suppression of protein synthesis [12]. To understand more the molecul ...
... metabolic link between mTOR and glycolysis has also been described where low glycolytic flux leads to binding of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to the mTORC1-regulator Rheb thereby inhibiting mTORC1 signaling and suppression of protein synthesis [12]. To understand more the molecul ...
2. CYCLIC AMINOACIDS 2.1. Aromatic
... PROTEINS The major cell components of any living organism (25% of wet weight and 45-50% of dry weight) Play important roles in all biological processes Elementary composition: C 51-55%, O 21-23%, N 15-18%, H 6-7%, S 0.3-2.5% Structure - they are – high-molecular (the mass of single-chain protein is ...
... PROTEINS The major cell components of any living organism (25% of wet weight and 45-50% of dry weight) Play important roles in all biological processes Elementary composition: C 51-55%, O 21-23%, N 15-18%, H 6-7%, S 0.3-2.5% Structure - they are – high-molecular (the mass of single-chain protein is ...