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The Malaria Parasite`s Chloroquine Resistance Transporter is a
The Malaria Parasite`s Chloroquine Resistance Transporter is a

... that the sequence similarity shared by the query protein and the retrieved protein arose by chance. A P value ,1024 is considered to indicate a significant sequence similarity between the two proteins, consistent with their having a related biological function. Following the Plasmodia proteins, the ...
Nutrients and Diet
Nutrients and Diet

... Rickets (child – bones do not form properly (bowlegs, knock-knees)). ...


... Choice A: The aminoacyl synthetase that attaches Ala to the correct tRNA can also, by mistake, attach the amino acid Gly. If Gly is attached, then it is removed by hydrolysis at a separate editing site on the enzyme. Based on the structure of these amino acids, provide a sketch or description of the ...
The Case Against a Darwinian Origin of Protein Folds
The Case Against a Darwinian Origin of Protein Folds

... chances of confusing any one amino acid for any other during protein synthesis, and (as already noted) we see that the products of this tightly controlled process are long proteins. Lastly, we see that these long proteins perform an impressive variety of functions with equally impressive specificity ...
The Purification and Characterization of the Highly Labeled
The Purification and Characterization of the Highly Labeled

... tivity of about 4,200 c.p.m. per absorbance unit. After dialysis and lyophilization the material was passed through a column of A-5 M agarose, which is capable of fractionating molecules in the molecular weight range of 5 x 105 to 5 x 10G, the smaller components requiring a larger volume for elution ...
From: Methods in Molecular Biology, vol. 408
From: Methods in Molecular Biology, vol. 408

... the three kingdoms of life, as well as to elaborate first identifiers of IDP function. The first predictor of ID regions was reported in 1997 (54). This two-layer feedforward neural network, which achieved a surprising accuracy of about 70% clearly marked the beginning of a new epoch by showing that ...
Journal of Bacteriology
Journal of Bacteriology

... NaCl in column buffer. Further purification of the Fe protein. The Fe protein fraction from the first DEAE-52 column was heat treated at 55°C for 5 min and cooled quickly, and denatured proteins were centrifuged down. The supernatant was diluted with three volumes of 50 mM Tris acetate buffer (pH 7. ...
Complete Mitochondrial DNA Sequence and Amino Acid Analysis of
Complete Mitochondrial DNA Sequence and Amino Acid Analysis of

... AF390098; Liverpool AeCOI, AY056596; Formosus AeCOI, AY056597 and Moyo-R AeCOI, AF380835. Comparative studies of mitochondrial DNA (mtDNA) among different groups have revealed an overall well conserved organization across metazoa but significant differences also exist. For example, compared to verte ...
Regulation of hepatic metabolism by AMPK - HAL
Regulation of hepatic metabolism by AMPK - HAL

... by phosphorylation of the carbohydrate response element binding protein (ChREBP), reducing its DNA binding capacity and nuclear translocation, and down-regulating sterol regulatory element-binding protein-1c (SREBP-1c) gene expression and stability probably through SIRT1-dependent deacetylation [1, ...
Full Article
Full Article

... In order to counteract oxidative stress and to maintain redox balance, enzymatic and non-enzymatic anti-oxidant systems have evolved. The products of genes that are induced in response to oxidative stress may confer protection against subsequent adversities or serve to signal stress to neighboring c ...
Protein - people.vcu.edu
Protein - people.vcu.edu

... A blueprint would solve these problems, specifying for each protein where it's supposed to go. This is not the answer nature found. There are no blueprints, and the protein must contain within itself information specifying its ultimate location. Since protein are nothing more than sequences of amino ...
Regulation of protein synthesis by insulin
Regulation of protein synthesis by insulin

... reveal that the S6Ks are not required for the normal control of 5 -TOP mRNA translation. Thus additional and so far unknown targets for mTOR signalling must be involved in modulating 5 -TOP mRNA translation (Figure 2). The ability of agents such as insulin to activate ribosomal protein biosynthesi ...
Binding of a Growth Hormone- Inducible Nuclear Factor Is Mediated
Binding of a Growth Hormone- Inducible Nuclear Factor Is Mediated

... an alternative pathway for GH-mediatedgene activation. The Spi 2.1 gene does not contain a sequence related to the consensusAP-l-binding site. However, Fos expressionitself is may be mediatedby a p91-like protein in a complex with other phosphoproteins.Thus, GH may activate both p91-type and MAP kin ...
Tissue-specific expression of AUX1 in maize roots
Tissue-specific expression of AUX1 in maize roots

... observed in mesocotyls and leaves (data not shown), as expected from the diverse actions of auxin in the whole plant. Interestingly however, ZmAUX1 expression could not be detected in coleoptiles, which are very responsive to exogenously applied auxin and have been extensively used for auxin transpo ...
pDsRed-Monomer-C1 Vector Information
pDsRed-Monomer-C1 Vector Information

... pDsRed-Monomer-C1 can be used to construct fusions to the C-terminus of DsRed-Monomer. If a fusion construct retains the fluorescent properties of the native DsRed-Monomer protein, its expression can be monitored by flow cytometry and its localization in vivo can be determined by fluorescence micros ...
Patrick Cramer Anton Meinhart, Tobias Silberzahn and
Patrick Cramer Anton Meinhart, Tobias Silberzahn and

... and remains phosphorylated during RNA chain elongation. Several CTD kinases have been described (4), but only one CTD phosphatase, called Fcp1, is known (5, 6). In addition to pol II, several other proteins of the transcription machinery are phosphorylated (7), and RNA processing complexes contain p ...
C1qRP Is a Heavily O-Glycosylated Cell Surface Protein Involved in
C1qRP Is a Heavily O-Glycosylated Cell Surface Protein Involved in

... modification of the technique of Lionetti et al. (19), as described (20). Greater than 95% of the cells in each preparation were monocytes according to size analysis on a Coulter Channelyzer (Hialeah, FL). Previous analysis has substantiated that such populations are nonspecific esterase positive an ...
An Efficient Protocol for Identifying Separation-of-Function
An Efficient Protocol for Identifying Separation-of-Function

... telomere length decline in a wild-type strain (i.e., in the presence of the wild-type Est3 protein expressed from its native genomic locus), whereas overexpression of the Est3W21A or Est3-D86A proteins had no impact (Lee et al. 2008 and Figure 1A; see also below). To examine whether this differentia ...
Goat Milk - Mt. Capra
Goat Milk - Mt. Capra

... less to say, protein is nothing less than essential for all aspects of life. An important concept to note, however, is that not all proteins are created equal. Far from it. In fact, an entire ratings system has been created to categorize the biological value (BV) of the protein in different substanc ...
Section L Regulation of Transcription in Prokaryotes
Section L Regulation of Transcription in Prokaryotes

... Transcription regulation patterns: • By transcriptional repressors: such as the lac repressor; • By transcriptional activators: such as the CRP; • By different s to direct RNApol binding different promoter: Functions of s factors :  The abb’w core enzyme of RNA polymerase is unable to start transcr ...
Genome-wide analysis of the GRAS gene family in
Genome-wide analysis of the GRAS gene family in

... development. Physic nut (Jatropha curcas L.) was found to have a total of 48 GRAS family members (JcGRAS), 15 more than those found in Arabidopsis. The JcGRAS genes were divided into 12 subfamilies or 15 ancient monophyletic lineages based on the phylogenetic analysis of GRAS proteins from both flow ...
results and discussion
results and discussion

... tools translates nucleotide sequences by using ...
TD3 5.08 – PKS/NRPS pathways and mass
TD3 5.08 – PKS/NRPS pathways and mass

... - biosynthesizes yersiniabactin (Ybt), an Fe III chelator - Ybt strips Fe3+ from host proteins and is used for Y pestis survival - Ybt synthesized by hybrid NRPS/PKS system D= Dalton = g/mol ...
Slide 1
Slide 1

... AS it is proved in past that machine learning techniques are elegant in classifying the biological data. Hau and sun applied the SVM for classification of prokaryotic and eukaryotic proteins and shown that it is better than statistical methods as well as other machine learning techniques such as AN ...
Analysis - The Journal of Cell Biology
Analysis - The Journal of Cell Biology

... The casein kinase I (CKI) proteins originally were characterized as ubiquitous serine/threonine kinases with a preference for acidic substrates such as casein. Although members of this family were among the first kinases purified, elucidating their function and regulation has been difficult. Recentl ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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