Industrial biotechnology: Tools and applications
... “Omics” and in silico approaches Increased genome sequencing efforts have ushered in a new era of systems biology, in which entire cellular networks are analyzed and optimized for application in the development of strains and bioprocesses. The properties of these complex cellular networks cannot be ...
... “Omics” and in silico approaches Increased genome sequencing efforts have ushered in a new era of systems biology, in which entire cellular networks are analyzed and optimized for application in the development of strains and bioprocesses. The properties of these complex cellular networks cannot be ...
Molecular cloning and characterization of an acyl
... To recover full length cDNA sequence of the AhFatB1 genes in allotetraploid peanut, PCR amplifications were conducted on cDNA derived from developing seed of four different peanut genotypes (Table 1). Primers were designed based on the putative 5’ and 3’-untranslated regions of the contigs we obtain ...
... To recover full length cDNA sequence of the AhFatB1 genes in allotetraploid peanut, PCR amplifications were conducted on cDNA derived from developing seed of four different peanut genotypes (Table 1). Primers were designed based on the putative 5’ and 3’-untranslated regions of the contigs we obtain ...
Protein Folding Cell and Mol Biology Lab
... (see end of this file for selected text pages on CDK2/cyclin, Calmodulin, protein folding diseases, prions and chaperones Protein Structure Sites: Protein Folding (Chapt. 3 of World of the Cell) and Enzymes (Chapt. 6): How a protein folds in 3D space is important for protein function. If a protein d ...
... (see end of this file for selected text pages on CDK2/cyclin, Calmodulin, protein folding diseases, prions and chaperones Protein Structure Sites: Protein Folding (Chapt. 3 of World of the Cell) and Enzymes (Chapt. 6): How a protein folds in 3D space is important for protein function. If a protein d ...
HiFliQ Ni-NTA FPLC Columns - User Guide
... cell pellet under native conditions using Protein Ark HiFliQ Ni-NTA FPLC Columns. Reagent amounts given apply to IPTG-induced bacterial culture of a well-expressed protein (approximately 10-50 mg/l). Cells are lysed with lysozyme because it is an inexpensive and efficient method for cells that have ...
... cell pellet under native conditions using Protein Ark HiFliQ Ni-NTA FPLC Columns. Reagent amounts given apply to IPTG-induced bacterial culture of a well-expressed protein (approximately 10-50 mg/l). Cells are lysed with lysozyme because it is an inexpensive and efficient method for cells that have ...
... B12. (10 pts) Please do one of the following three choices. Please indicate the choice that you are answering. Choice A: Individuals with glycogen storage diseases are often missing the enzyme glycogen phosphorylase. i) How would this deficiency affect the liver's ability to respond to epinephrine? ...
How to study proteins by circular dichroism
... Keywords: Circular dichroism; Protein structure; Secondary structure; Protein folding; Ligand binding ...
... Keywords: Circular dichroism; Protein structure; Secondary structure; Protein folding; Ligand binding ...
How to study proteins by circular dichroism
... Keywords: Circular dichroism; Protein structure; Secondary structure; Protein folding; Ligand binding ...
... Keywords: Circular dichroism; Protein structure; Secondary structure; Protein folding; Ligand binding ...
Quantitative Proteomics of Transporter Expression in Brain Capillary
... whether genetic modification of one transporter causes changes in regulation and/or expression (up or down) of other critical transporter proteins that may influence their functional role in drug disposition to the brain. Certainly, up-regulation of P-gp in the Bcrp1(⫺/⫺) mice could, in part, explai ...
... whether genetic modification of one transporter causes changes in regulation and/or expression (up or down) of other critical transporter proteins that may influence their functional role in drug disposition to the brain. Certainly, up-regulation of P-gp in the Bcrp1(⫺/⫺) mice could, in part, explai ...
analyzing gene and protein sequences
... hemoglobin may polymerize, especially under low oxygen conditions, and cause sickling of red blood cells. The normally doughnut-shaped red blood cells can move through blood vessels with ease. Abnormal sickle cells may get stuck in the narrow blood vessels. The inability of the blood cells to flow t ...
... hemoglobin may polymerize, especially under low oxygen conditions, and cause sickling of red blood cells. The normally doughnut-shaped red blood cells can move through blood vessels with ease. Abnormal sickle cells may get stuck in the narrow blood vessels. The inability of the blood cells to flow t ...
subunits of succinyl CoA ligase of tomato
... Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNA ...
... Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNA ...
Detailed Supporting Information
... debris was removed by centrifugation and the resulting cell extract (supernatant) was immediately used for enzyme assays or stored at -20C. All operations were carried out on ice. The supernatant of this sample was used to determine total protein concentration in enzyme samples using standard Brad ...
... debris was removed by centrifugation and the resulting cell extract (supernatant) was immediately used for enzyme assays or stored at -20C. All operations were carried out on ice. The supernatant of this sample was used to determine total protein concentration in enzyme samples using standard Brad ...
Restriction fragment differential display of pediocin
... Fig. 1. Schematic outline of the RFDD-PCR technology. After synthesis from total RNA, the double-stranded cDNA is digested with TaqI. Following digestion, two specially designed DNA adaptors (see enlargement in box) are ligated to the cDNA fragments. Adaptor ligation has three possible outcomes. One ...
... Fig. 1. Schematic outline of the RFDD-PCR technology. After synthesis from total RNA, the double-stranded cDNA is digested with TaqI. Following digestion, two specially designed DNA adaptors (see enlargement in box) are ligated to the cDNA fragments. Adaptor ligation has three possible outcomes. One ...
Alfalfa Cyclins: Differential Expression during the Cell
... suspension cultured cells that were induced to form somatic embryos with redundant oligonucleotides encoding the conserved amino acid sequences K-Y-E-E-M(I)-Y-Pfor the cyclin A and B type and I-L-V(I)-D-W-L-Vfor the cyclin B class (see Methods for the nucleotide sequences). Positive colonies were is ...
... suspension cultured cells that were induced to form somatic embryos with redundant oligonucleotides encoding the conserved amino acid sequences K-Y-E-E-M(I)-Y-Pfor the cyclin A and B type and I-L-V(I)-D-W-L-Vfor the cyclin B class (see Methods for the nucleotide sequences). Positive colonies were is ...
Fatty Acid Biosynthesis Pathways in Methylomicrobium buryatense
... Methane utilization by methanotrophic bacteria is an attractive application for biotechnological conversion of natural or biogas into high-added-value products. Haloalcaliphilic methanotrophic bacteria belonging to the genus Methylomicrobium are among the most promising strains for methane-based bio ...
... Methane utilization by methanotrophic bacteria is an attractive application for biotechnological conversion of natural or biogas into high-added-value products. Haloalcaliphilic methanotrophic bacteria belonging to the genus Methylomicrobium are among the most promising strains for methane-based bio ...
Cardiac Energy Dependence on Glucose Increases
... Background-—Long chain acyl-CoA synthetases (ACSL) catalyze long-chain fatty acids (FA) conversion to acyl-CoAs. Temporal ACSL1 inactivation in mouse hearts (Acsl1H/) impaired FA oxidation and dramatically increased glucose uptake, glucose oxidation, and mTOR activation, resulting in cardiac hyper ...
... Background-—Long chain acyl-CoA synthetases (ACSL) catalyze long-chain fatty acids (FA) conversion to acyl-CoAs. Temporal ACSL1 inactivation in mouse hearts (Acsl1H/) impaired FA oxidation and dramatically increased glucose uptake, glucose oxidation, and mTOR activation, resulting in cardiac hyper ...
Applied and Environmental Microbiologyy
... Recent reports have shown that several strains of Pseudomonas putida produce N-acylhomoserine lactones (AHLs). These signal molecules enable bacteria to coordinately express certain phenotypic traits in a densitydependent manner in a process referred to as quorum sensing. In this study we have clone ...
... Recent reports have shown that several strains of Pseudomonas putida produce N-acylhomoserine lactones (AHLs). These signal molecules enable bacteria to coordinately express certain phenotypic traits in a densitydependent manner in a process referred to as quorum sensing. In this study we have clone ...
Rumen Protected Fat
... Coating of fibrous portion of diet with lipids Modification in cellulose degrading bacteria Toxic to cellulolytic bacteria ...
... Coating of fibrous portion of diet with lipids Modification in cellulose degrading bacteria Toxic to cellulolytic bacteria ...
Distribution of major serum proteins in an airbrea
... The total number of protein bands in PAGE profiles of C. punctata unfractioned serum (control) was visually counted as seventeen (Fig. 1A). Overloading obscured clear resolution of fastest migrating β-globulin bands (Fig. 1A, lanes 1,4 and 5) labeled as transferrins (Trf), while that labeled as albu ...
... The total number of protein bands in PAGE profiles of C. punctata unfractioned serum (control) was visually counted as seventeen (Fig. 1A). Overloading obscured clear resolution of fastest migrating β-globulin bands (Fig. 1A, lanes 1,4 and 5) labeled as transferrins (Trf), while that labeled as albu ...
Proteins in Serum and Urine - Ústav lékařské biochemie a
... presence of surface-active substances. Organic dyes are used that react with albumin much faster than with other proteins. They usually contain -SO3H group. Bromcresol purple or bromcresol green can serve as examples. Reference values (S-Albumin): 35 – 53 g/l For estimation of albumin in other fluid ...
... presence of surface-active substances. Organic dyes are used that react with albumin much faster than with other proteins. They usually contain -SO3H group. Bromcresol purple or bromcresol green can serve as examples. Reference values (S-Albumin): 35 – 53 g/l For estimation of albumin in other fluid ...
Slide 1
... Glucose synthesis Glucose biosynthesis Glucose formation Glucose anabolism Gluconeogenesis ...
... Glucose synthesis Glucose biosynthesis Glucose formation Glucose anabolism Gluconeogenesis ...
Response of Jujube Fruits to Exogenous Oxalic Acid Treatment
... treatment. In order to determine the details of protein spots in Fig. 5, we magnified the areas of protein spots of interest in Fig. 7A, in which the marked numbers of the protein spots are the same as those in Fig. 5. Fig. 7B shows the abundance of the proteins which are involved in metabolism and e ...
... treatment. In order to determine the details of protein spots in Fig. 5, we magnified the areas of protein spots of interest in Fig. 7A, in which the marked numbers of the protein spots are the same as those in Fig. 5. Fig. 7B shows the abundance of the proteins which are involved in metabolism and e ...
Insulin mRNA to Protein Kit© A 3DMD Paper BioInformatics and Mini
... (exon) is interrupted by two intervening sequences (introns). Introns are copied into the precursor mRNA by the RNA polymerase that transcribes the β-globin gene. These introns are then spliced out of the precursor mRNA as it is being transported to the cytoplasm where the mature mRNA will be transl ...
... (exon) is interrupted by two intervening sequences (introns). Introns are copied into the precursor mRNA by the RNA polymerase that transcribes the β-globin gene. These introns are then spliced out of the precursor mRNA as it is being transported to the cytoplasm where the mature mRNA will be transl ...
Plant Physiology, Fifth Edition
... Plant Life: Unifying Principles 2 Overview of Plant Structure 2 Plant cells are surrounded by rigid cell walls 2 New cells are produced by dividing tissues called meristems 2 Three major tissue systems make up the plant body 4 Plant Cell Organelles 4 Biological membranes are phospholipid bilayers th ...
... Plant Life: Unifying Principles 2 Overview of Plant Structure 2 Plant cells are surrounded by rigid cell walls 2 New cells are produced by dividing tissues called meristems 2 Three major tissue systems make up the plant body 4 Plant Cell Organelles 4 Biological membranes are phospholipid bilayers th ...