Lecture 1 - Edward Dennis - University of California San Diego
... Department of Pharmacology, School of Medicine University of California, San Diego Copyright/attribution notice: You are free to copy, distribute, adapt and transmit this tutorial or individual slides (without alteration) for academic, non-profit and non-commercial purposes. Attribution: Edward A. D ...
... Department of Pharmacology, School of Medicine University of California, San Diego Copyright/attribution notice: You are free to copy, distribute, adapt and transmit this tutorial or individual slides (without alteration) for academic, non-profit and non-commercial purposes. Attribution: Edward A. D ...
Protein
... Albumins - They are soluble in water and dilute solutions of salts. They are widely distributed in nature, mostly seen in seeds. They are coagulated by heat. Example: β-Amylose Albumin of Barley, Leucosine, legumeline, serum albumin. Also in egg white, plasma of blood, milk. Globulins - They are alm ...
... Albumins - They are soluble in water and dilute solutions of salts. They are widely distributed in nature, mostly seen in seeds. They are coagulated by heat. Example: β-Amylose Albumin of Barley, Leucosine, legumeline, serum albumin. Also in egg white, plasma of blood, milk. Globulins - They are alm ...
22 Virus Bacteria AP Bio 2015
... The basic “life” cycle of virus particles relies on using the basic machinery of host cells to replicate more virus particles. Virus has no Ribosomes or other organelles that could allow them to isolate specific functions within their capsid, and this perhaps prevents them from being more complex. H ...
... The basic “life” cycle of virus particles relies on using the basic machinery of host cells to replicate more virus particles. Virus has no Ribosomes or other organelles that could allow them to isolate specific functions within their capsid, and this perhaps prevents them from being more complex. H ...
Hierarchical DNA Sequencing
... the process of obtaining many reads sequencing from random locations in DNA, to detect overlaps and assemble CS262 Lecture 9, Win07, Batzoglou ...
... the process of obtaining many reads sequencing from random locations in DNA, to detect overlaps and assemble CS262 Lecture 9, Win07, Batzoglou ...
gene mutation -unit-2-study mat-2012
... drastically altered. Since translation takes place in the 5’.3’ direction, a frameshift mutation near the 3’ end of the gene results in only the terminal part orthe polypeptide chain being altered. This may result in a functional protein. The several variants of haemoglobin are belived to have arise ...
... drastically altered. Since translation takes place in the 5’.3’ direction, a frameshift mutation near the 3’ end of the gene results in only the terminal part orthe polypeptide chain being altered. This may result in a functional protein. The several variants of haemoglobin are belived to have arise ...
Ketoglutarate Dehydrogenase Complex
... isolated and sequenced, so that the amino acid sequence (primary structure) for each protein is known. E1k is 2-oxoglutarate decarboxylase, and is also referred to as 2oxoacid decarboxylase (EC 1.2.4.2). The human protein contains 962 amino acids linked to a 40-amino acid leader sequence.6–8 E2k is ...
... isolated and sequenced, so that the amino acid sequence (primary structure) for each protein is known. E1k is 2-oxoglutarate decarboxylase, and is also referred to as 2oxoacid decarboxylase (EC 1.2.4.2). The human protein contains 962 amino acids linked to a 40-amino acid leader sequence.6–8 E2k is ...
7.1 The lac Operon
... heparin—a polyanion that binds to any RNA polymerase that is free or loosely bound to DNA and keeps it from binding to DNA. They also added all the remaining components of the RNA polymerase reaction except CTP. ...
... heparin—a polyanion that binds to any RNA polymerase that is free or loosely bound to DNA and keeps it from binding to DNA. They also added all the remaining components of the RNA polymerase reaction except CTP. ...
Subunit Isoform of X,K-ATPase in Human Skeletal Muscle
... In our previous paper we described homologous human and rat cDNAs encoding a novel X,K-ATPase -subunit (4). RT-PCR showed that the corresponding gene has a highly specific expression pattern being active only in skeletal muscle and at a low level in heart, and silent in other tissues tested. To emp ...
... In our previous paper we described homologous human and rat cDNAs encoding a novel X,K-ATPase -subunit (4). RT-PCR showed that the corresponding gene has a highly specific expression pattern being active only in skeletal muscle and at a low level in heart, and silent in other tissues tested. To emp ...
Using virus-induced gene silencing to identify novel regulators of
... After two weeks in the growth chambers, gene silencing in some of the plants was evident by a change in phenotype. At this time, all plants were bombarded with GFP DNA. The vectors containing 1x GFP and 2x GFP DNA were first scraped off a plate into a flask with a 500 ml solution of LB medium with t ...
... After two weeks in the growth chambers, gene silencing in some of the plants was evident by a change in phenotype. At this time, all plants were bombarded with GFP DNA. The vectors containing 1x GFP and 2x GFP DNA were first scraped off a plate into a flask with a 500 ml solution of LB medium with t ...
Biocatalytic degradation of pollutants
... ADP and Agrobacterium radiobacter J14a [21]. Likewise, Ralstonia eutropha JMP134(pJP4), which grows on the herbicide 2,4-dichlorophenoxyacetate (2,4D), showed an inducible chemotactic response to 2,4D [22]. The response required the presence of the TfdK permease, a member of the major facilitator su ...
... ADP and Agrobacterium radiobacter J14a [21]. Likewise, Ralstonia eutropha JMP134(pJP4), which grows on the herbicide 2,4-dichlorophenoxyacetate (2,4D), showed an inducible chemotactic response to 2,4D [22]. The response required the presence of the TfdK permease, a member of the major facilitator su ...
Analysis of expressed sequence tags from Cryptomeria japonica
... proteins that were not homologous to any proteins in current databases. The CjMP1-related genes formed a multi-gene family and were expressed specifically in the pollen grains of C. japonica. An analysis of homologies between ESTs from C. japonica pollen and proteins in the Structural Database of Al ...
... proteins that were not homologous to any proteins in current databases. The CjMP1-related genes formed a multi-gene family and were expressed specifically in the pollen grains of C. japonica. An analysis of homologies between ESTs from C. japonica pollen and proteins in the Structural Database of Al ...
The complete mitochondrial genome of Channa argus, Channa
... Ala, Asn, Cys, Tyr, Ser, Glu and Pro) located on the L-strand, and all genes were similar in length to those in other bony fishes (Miya et al. 2003). The gene order was identical to that obtained for other vertebrates (Guo et al. 2004). The number of encoding amino acid for protein-coding genes (PGC ...
... Ala, Asn, Cys, Tyr, Ser, Glu and Pro) located on the L-strand, and all genes were similar in length to those in other bony fishes (Miya et al. 2003). The gene order was identical to that obtained for other vertebrates (Guo et al. 2004). The number of encoding amino acid for protein-coding genes (PGC ...
Novel surface layer protein genes in Bacillus
... performed on positively charged nylon membranes (Roche) using a TurboBlotter (Schleicher & Schuell), following the manufacturer’s instructions. Northern blotting of total RNA was performed according to published methods (Sambrook et al., 1989), by applying 2 mg total RNA of Cd2+-treated cells, and 5 ...
... performed on positively charged nylon membranes (Roche) using a TurboBlotter (Schleicher & Schuell), following the manufacturer’s instructions. Northern blotting of total RNA was performed according to published methods (Sambrook et al., 1989), by applying 2 mg total RNA of Cd2+-treated cells, and 5 ...
Evolutionary Relationships and Protein Domain Architecture in an
... small subunit. The sequence of domain IV is related to calmodulin, leading to the hypothesis that calpains are modular and have evolved by fusion of a calmodulin-like gene with a protease precursor domain. In addition to typical calpains that conform to the four-domain structure, unconventional calp ...
... small subunit. The sequence of domain IV is related to calmodulin, leading to the hypothesis that calpains are modular and have evolved by fusion of a calmodulin-like gene with a protease precursor domain. In addition to typical calpains that conform to the four-domain structure, unconventional calp ...
Cholesterol, steroids, and related molecules
... These general principles can be used to rationalize two examples of normal tissuespecific hormone synthesis, and one pathological state, congenital adrenal hyperplasia due to 21 alpha hydroxylase deficiency. Cortisol biosynthesis. Cortisol is a glucocorticoid hormone, activating the transcription of ...
... These general principles can be used to rationalize two examples of normal tissuespecific hormone synthesis, and one pathological state, congenital adrenal hyperplasia due to 21 alpha hydroxylase deficiency. Cortisol biosynthesis. Cortisol is a glucocorticoid hormone, activating the transcription of ...
insulin history
... Part II – The Discovery of Insulin Before insulin was discovered, being diagnosed with type 1 (insulin dependent) diabetes was a death sentence. Type 1 diabetes leads to high blood glucose levels (hyperglycemia), which can damage the kidneys, the brain, the cardiovascular system, the eyes (leading t ...
... Part II – The Discovery of Insulin Before insulin was discovered, being diagnosed with type 1 (insulin dependent) diabetes was a death sentence. Type 1 diabetes leads to high blood glucose levels (hyperglycemia), which can damage the kidneys, the brain, the cardiovascular system, the eyes (leading t ...
Molecular Cloning, Sequencing, and Expression of the Glutamine
... had a G+C content of 68.7%, which agrees with an estimated total G+C content of 68.4 to 72.1% for Frankia strains in general (2). The genetic code permits the use of G or C in the third position in codons for all 20 amino acids and in the first position for Arg and Leu; no choice exists in the secon ...
... had a G+C content of 68.7%, which agrees with an estimated total G+C content of 68.4 to 72.1% for Frankia strains in general (2). The genetic code permits the use of G or C in the third position in codons for all 20 amino acids and in the first position for Arg and Leu; no choice exists in the secon ...
WebMOTIFS: Web-based integrated motif discovery
... several microarray platforms for S. cerevisiae, M. musculus, and H. sapiens. WebMOTIFS automates all the remaining steps and sends the user an email with a link to the results, which are kept on our server for 30 days. In addition to graphical output, the data can be downloaded in text-based formats ...
... several microarray platforms for S. cerevisiae, M. musculus, and H. sapiens. WebMOTIFS automates all the remaining steps and sends the user an email with a link to the results, which are kept on our server for 30 days. In addition to graphical output, the data can be downloaded in text-based formats ...
Volume 18 - Number 9 September 2014 Atlas of Genetics and Cytogenetics
... contains a proline rich, SH3 domain binding motif, three tyrosine containing SH2 domain binding sites (Xu et al., 2007), of which a YXXM motif is for PI3 kinase subunit p85 binding (Lodyga et al., 2009). In the middle region, there are two pleckstrin homology domains and another tyrosine binding mot ...
... contains a proline rich, SH3 domain binding motif, three tyrosine containing SH2 domain binding sites (Xu et al., 2007), of which a YXXM motif is for PI3 kinase subunit p85 binding (Lodyga et al., 2009). In the middle region, there are two pleckstrin homology domains and another tyrosine binding mot ...
K-12 MG1655 Escherichia coli Blocks the Aerobic
... cyoABCD, and cbdAB) and a quinol monooxygenase gene (ygiN) from the E. coli K-12 MG1655 genome resulted in the activation of ArcA aerobically. These mutations resulted in reduction of the oxygen uptake rate by nearly 98% and production of D-lactate as a sole by-product under oxic and anoxic conditio ...
... cyoABCD, and cbdAB) and a quinol monooxygenase gene (ygiN) from the E. coli K-12 MG1655 genome resulted in the activation of ArcA aerobically. These mutations resulted in reduction of the oxygen uptake rate by nearly 98% and production of D-lactate as a sole by-product under oxic and anoxic conditio ...
Alternative isoleucine synthesis pathway in
... Cyanothece sp. ATCC 51142 is an aerobic N2-fixing and hydrogen-producing cyanobacterium. Isotopomer analysis of its amino acids revealed an identical labelling profile for leucine and isoleucine when Cyanothece 51142 was grown mixotrophically using 2-13C-labelled glycerol as the main carbon source. ...
... Cyanothece sp. ATCC 51142 is an aerobic N2-fixing and hydrogen-producing cyanobacterium. Isotopomer analysis of its amino acids revealed an identical labelling profile for leucine and isoleucine when Cyanothece 51142 was grown mixotrophically using 2-13C-labelled glycerol as the main carbon source. ...
Research Project Final Report
... the wild C genome species and the B.napus diversity sets. The sequences were analysed for changes which may affect protein structure and hence enzyme function i.e. have an effect on fatty acid profile and content. A significant number of different alleles were identified for each gene. The wild C ge ...
... the wild C genome species and the B.napus diversity sets. The sequences were analysed for changes which may affect protein structure and hence enzyme function i.e. have an effect on fatty acid profile and content. A significant number of different alleles were identified for each gene. The wild C ge ...
Evolution of Cellular Data Processing
... 3.2.2 Group translocation systems: primeval forms of dataprocessing networks combining food uptake with gene regulation The best-known representatives of this family of membrane transporters are the PEP (phosphoenol pyruvate)-powered phosphotransferase systems, abbreviated as PTS. They combine membr ...
... 3.2.2 Group translocation systems: primeval forms of dataprocessing networks combining food uptake with gene regulation The best-known representatives of this family of membrane transporters are the PEP (phosphoenol pyruvate)-powered phosphotransferase systems, abbreviated as PTS. They combine membr ...
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... slight green hue (Eugster and Marki-Fischer, 1991). Betalains also have a rather limited distribution, as they are found exclusively in the Caryophyllales, a group of angiosperms that include beetroot (Stafford, 1994). Despite this limited distribution, betalains can assume a wide range of colours f ...
... slight green hue (Eugster and Marki-Fischer, 1991). Betalains also have a rather limited distribution, as they are found exclusively in the Caryophyllales, a group of angiosperms that include beetroot (Stafford, 1994). Despite this limited distribution, betalains can assume a wide range of colours f ...
Dynamics of the trp Operon
... * Cluster of genes controlled by a single (?) feedback regulatory mechanism. ...
... * Cluster of genes controlled by a single (?) feedback regulatory mechanism. ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.