SURVEY AND SUMMARY Regulation of mammalian nucleotide
... To sustain energy and precursors for RNA biosynthesis, the ATP concentration in cells is generally maintained above 1 mM (71) with a high ATP/ADP.Pi ratio (72), so that there is sufficient free energy available to drive endergonic reactions. There are limits to the maintenance of these levels, below ...
... To sustain energy and precursors for RNA biosynthesis, the ATP concentration in cells is generally maintained above 1 mM (71) with a high ATP/ADP.Pi ratio (72), so that there is sufficient free energy available to drive endergonic reactions. There are limits to the maintenance of these levels, below ...
Expanding the Genetic Code of Escherichia coli
... A unique transfer RNA (tRNA)/aminoacyl-tRNA synthetase pair has been generated that expands the number of genetically encoded amino acids in Escherichia coli. When introduced into E. coli, this pair leads to the in vivo incorporation of the synthetic amino acid O-methyl-L-tyrosine into protein in re ...
... A unique transfer RNA (tRNA)/aminoacyl-tRNA synthetase pair has been generated that expands the number of genetically encoded amino acids in Escherichia coli. When introduced into E. coli, this pair leads to the in vivo incorporation of the synthetic amino acid O-methyl-L-tyrosine into protein in re ...
Metabolic and physiological interdependencies in the
... of chemosynthetic Gammaproteobacteria in its gills: a sulfur oxidizer and a methane oxidizer. Despite numerous investigations over the last decades, the degree of interdependence between the three symbiotic partners, their individual metabolic contributions, as well as the mechanism of carbon transf ...
... of chemosynthetic Gammaproteobacteria in its gills: a sulfur oxidizer and a methane oxidizer. Despite numerous investigations over the last decades, the degree of interdependence between the three symbiotic partners, their individual metabolic contributions, as well as the mechanism of carbon transf ...
One gene, many neuropsychiatric disorders
... of genetic correlation among disorders, it is unlikely to explain the high genetic correlation observed between bipolar disorder and schizophrenia6,7. In addition, the shared genetic risk of multiple neuropsychiatric disorders is reminiscent of what is observed for autoimmune disorders2. Moreover, m ...
... of genetic correlation among disorders, it is unlikely to explain the high genetic correlation observed between bipolar disorder and schizophrenia6,7. In addition, the shared genetic risk of multiple neuropsychiatric disorders is reminiscent of what is observed for autoimmune disorders2. Moreover, m ...
1. SVM example: Computational Biology Assume a fixed species
... 1. SVM example: Computational Biology Assume a fixed species f (e.g. baker's yeast, s. cerevisae) has genome Z (collection of genes). Typically a transcription factor (TF) > binds to the promoter (upstream) DNA near 1 and initiates transcription. In this case we say 1 is a target of >. Question: giv ...
... 1. SVM example: Computational Biology Assume a fixed species f (e.g. baker's yeast, s. cerevisae) has genome Z (collection of genes). Typically a transcription factor (TF) > binds to the promoter (upstream) DNA near 1 and initiates transcription. In this case we say 1 is a target of >. Question: giv ...
Week Date Hours Topic Standard IBO Assessment Statement Labs
... synovial fluid, joint capsule, named bones and antagonistic muscles (biceps and triceps). Outline the functions of the structures in the human elbow joint named in 11.2.2. Compare the movements of the hip joint and the knee joint. Describe the structure of striated muscle fibers, including the myofi ...
... synovial fluid, joint capsule, named bones and antagonistic muscles (biceps and triceps). Outline the functions of the structures in the human elbow joint named in 11.2.2. Compare the movements of the hip joint and the knee joint. Describe the structure of striated muscle fibers, including the myofi ...
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families
... One major challenge is to develop effective methods for assigning specific functions to proteins within large families and for classifying family members into groups that share functional characteristics. While multiple sequence alignments can be incredibly useful in predicting functionally or struc ...
... One major challenge is to develop effective methods for assigning specific functions to proteins within large families and for classifying family members into groups that share functional characteristics. While multiple sequence alignments can be incredibly useful in predicting functionally or struc ...
GFP - Bio-Rad
... Developed over five years, Biotechnology Explorer kits and curricula have been written for teachers, by teachers, and have been extensively field-tested in a broad range of classroom settings from high school through the undergraduate level. Easy-to-use Biotechnology Explorer kits are the perfect wa ...
... Developed over five years, Biotechnology Explorer kits and curricula have been written for teachers, by teachers, and have been extensively field-tested in a broad range of classroom settings from high school through the undergraduate level. Easy-to-use Biotechnology Explorer kits are the perfect wa ...
Comparative genomics provides evidence for the 3
... and 62% identical to each other at the amino acid level respectively. The accA and accD most closely related to the sequences in C. aurantiacus are reported in Table 2. The colocalization of an accC gene downstream of accDA in the Roseiflexus sp. RS-1 and R. castenholzii genomes provided evidence th ...
... and 62% identical to each other at the amino acid level respectively. The accA and accD most closely related to the sequences in C. aurantiacus are reported in Table 2. The colocalization of an accC gene downstream of accDA in the Roseiflexus sp. RS-1 and R. castenholzii genomes provided evidence th ...
Complete DNA sequence of yeast chromosome II.
... genetic information of a number of organisms by establishing detailed maps and finally complete sequences of their genomes. With the present level of sequencing methodology, early efforts at genome sequencing have been concentrated on organisms with less complex genomes. In this context, model organ ...
... genetic information of a number of organisms by establishing detailed maps and finally complete sequences of their genomes. With the present level of sequencing methodology, early efforts at genome sequencing have been concentrated on organisms with less complex genomes. In this context, model organ ...
Acetyl-coenzyme A carboxylase – an attractive enzyme for biotechnolo
... (Davis et al., 2000). All genes were assembled in an artificial operon under bacteriophage T7 promoter. Grouping all genes under the same tightly controlled, inducible promoter on a low copy plasmid was essential for the project as an abnormal ratio and high concentration of ACCase subunits are harm ...
... (Davis et al., 2000). All genes were assembled in an artificial operon under bacteriophage T7 promoter. Grouping all genes under the same tightly controlled, inducible promoter on a low copy plasmid was essential for the project as an abnormal ratio and high concentration of ACCase subunits are harm ...
Acetyl-coenzyme A carboxylase – an attractive enzyme for biotechnolo
... (Davis et al., 2000). All genes were assembled in an artificial operon under bacteriophage T7 promoter. Grouping all genes under the same tightly controlled, inducible promoter on a low copy plasmid was essential for the project as an abnormal ratio and high concentration of ACCase subunits are harm ...
... (Davis et al., 2000). All genes were assembled in an artificial operon under bacteriophage T7 promoter. Grouping all genes under the same tightly controlled, inducible promoter on a low copy plasmid was essential for the project as an abnormal ratio and high concentration of ACCase subunits are harm ...
A General Method Applicable to the Search for Similarities in the
... are followed, the maximum match mill be the same. The cells of the array whi& contributed to the maximum match, may be determined by recording the origin of the number that mas added to each cell when the array was operated upon. 3. Evaluating the Significance of the Maximum Match A given maximum ma ...
... are followed, the maximum match mill be the same. The cells of the array whi& contributed to the maximum match, may be determined by recording the origin of the number that mas added to each cell when the array was operated upon. 3. Evaluating the Significance of the Maximum Match A given maximum ma ...
OPTIMIZER: a web server for optimizing the codon usage of DNA
... highly expressed genes usually use the minimal subset of codons that are recognized by the most abundant tRNA species, their ENc values are expected to be low. Figure 1 shows some of the outputs provided by the optimization of a DNA sequence: for example, the query and optimized sequences and an ali ...
... highly expressed genes usually use the minimal subset of codons that are recognized by the most abundant tRNA species, their ENc values are expected to be low. Figure 1 shows some of the outputs provided by the optimization of a DNA sequence: for example, the query and optimized sequences and an ali ...
Powerpoint - Wishart Research Group
... EWILLITALLCEASE >P12348 Sequence 3 MQWERTGHFDALKPQWERTYHEREISANTHERS... ...
... EWILLITALLCEASE >P12348 Sequence 3 MQWERTGHFDALKPQWERTYHEREISANTHERS... ...
Spnr, a Murine RNA-binding Protein That Is Localized to
... A eDNA probe containing a 3-kb insert corresponding to the Spnrgene was labeled with biotin-ll-dUTP by nick translation (GIBCO BRL, Gaithersburg, MD). The size of the product was determined to be between 200 and 400 bp. Metaphase chromosome preparations from lymphocytes of a male C57BL/6J mouse and ...
... A eDNA probe containing a 3-kb insert corresponding to the Spnrgene was labeled with biotin-ll-dUTP by nick translation (GIBCO BRL, Gaithersburg, MD). The size of the product was determined to be between 200 and 400 bp. Metaphase chromosome preparations from lymphocytes of a male C57BL/6J mouse and ...
Results - BioMed Central
... classification for all Unigenes and to understand the distribution of gene functions of ...
... classification for all Unigenes and to understand the distribution of gene functions of ...
FEMS Microbiology Letters
... E. coli auxotroph lacking cysteine synthase loci could be complemented with A. brasilense Sp7 cysK. The 3.0-kb HindIII-EcoRI fragment bearing cysK contained two additional ORFs encoding a putative transcriptional regulator and dUTPase. Insertional disruption of the cysK gene did not produce a cystei ...
... E. coli auxotroph lacking cysteine synthase loci could be complemented with A. brasilense Sp7 cysK. The 3.0-kb HindIII-EcoRI fragment bearing cysK contained two additional ORFs encoding a putative transcriptional regulator and dUTPase. Insertional disruption of the cysK gene did not produce a cystei ...
Characterisation of the Aspergillus niger dapB gene, which encodes
... transformants compared to the control strain. This increase is clearly significant and shows that increased dapB mRNA levels correlate with higher DPP IV activity. Measurements of DPP IV activity in the culture supernatant of the transformants did not detect any Fig. 3 a, b Analysis of the dapB trans ...
... transformants compared to the control strain. This increase is clearly significant and shows that increased dapB mRNA levels correlate with higher DPP IV activity. Measurements of DPP IV activity in the culture supernatant of the transformants did not detect any Fig. 3 a, b Analysis of the dapB trans ...
Specialized Functions of Major Histocompatibility Complex Class I
... M the polymorphic H-2 K, D, and L genes, located in the centromeric segment of the H-2 complex (1, 2), and the more numerous and less polymorphic "nonclassical" telomeric genes encoded in the H-2 Q, T, and M (formerly Qa-TlaHmt) regions (2). While products of the former are known to bind and present ...
... M the polymorphic H-2 K, D, and L genes, located in the centromeric segment of the H-2 complex (1, 2), and the more numerous and less polymorphic "nonclassical" telomeric genes encoded in the H-2 Q, T, and M (formerly Qa-TlaHmt) regions (2). While products of the former are known to bind and present ...
Costs of life - Dynamics of the protein inventory of
... of data-independent acquisition methods such as LC-MSE 20,21. Complemented with the Hi3 absolute quantification18 even more comprehensive quantitative data sets can be generated22. However, there are only very few studies that combine the dynamics of the entire protein inventory with interpretation ...
... of data-independent acquisition methods such as LC-MSE 20,21. Complemented with the Hi3 absolute quantification18 even more comprehensive quantitative data sets can be generated22. However, there are only very few studies that combine the dynamics of the entire protein inventory with interpretation ...
Heat shock response in hyperthermophilic microorganisms
... number of viable cells decreases exponentially with time, and this rate of decline increases exponentially with increasing temperature [32]. However, if a culture is exposed to a mild hyperthermal stress temperature prior to exposure to a more lethal temperature, then the number of viable cells in t ...
... number of viable cells decreases exponentially with time, and this rate of decline increases exponentially with increasing temperature [32]. However, if a culture is exposed to a mild hyperthermal stress temperature prior to exposure to a more lethal temperature, then the number of viable cells in t ...
3-1 Cyclin-Dependent Kinases
... shared by all protein kinases. By definition, all Cdks share the feature that their enzymatic activation requires the binding of a regulatory cyclin subunit. In most cases, full activation also requires phosphorylation of a threonine residue near the kinase active site. Although originally identifie ...
... shared by all protein kinases. By definition, all Cdks share the feature that their enzymatic activation requires the binding of a regulatory cyclin subunit. In most cases, full activation also requires phosphorylation of a threonine residue near the kinase active site. Although originally identifie ...
A Study of the Asp110–Glu112 Region of EcoRII Restriction
... EcoRII restriction endonuclease belongs to the type IIe restriction enzymes, which need the recognition of two DNA sites for their catalytic act [9]. The active enzyme–substrate complex consists of two identical subunits of EcoRII endonuclease interacting with two specific DNA sites [10]. But the cr ...
... EcoRII restriction endonuclease belongs to the type IIe restriction enzymes, which need the recognition of two DNA sites for their catalytic act [9]. The active enzyme–substrate complex consists of two identical subunits of EcoRII endonuclease interacting with two specific DNA sites [10]. But the cr ...
Polyunsaturated fatty acids stimulate hepatic UCP
... ever, adult humans have very little brown adipose tissue, making it unlikely to be a major contributor to human weight regulation. The discovery of UCP-2 and UCP-3, which are more widely expressed, has revived this hypothesis. UCP-2, like UCP-1, has been shown to be able to dissipate the proton grad ...
... ever, adult humans have very little brown adipose tissue, making it unlikely to be a major contributor to human weight regulation. The discovery of UCP-2 and UCP-3, which are more widely expressed, has revived this hypothesis. UCP-2, like UCP-1, has been shown to be able to dissipate the proton grad ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.