Biotechnology Explorer - Bio-Rad
... In this activity, students will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to one large chromosome, bacteria naturally contain one or more small circular pieces of DNA called plasmids. Plasmid DNA usually contains genes for one or more ...
... In this activity, students will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to one large chromosome, bacteria naturally contain one or more small circular pieces of DNA called plasmids. Plasmid DNA usually contains genes for one or more ...
Metabolic Engineering to Modify Flower Color
... Modification of the anthocyanidins mainly with glycosylation and acylation results in a wide variety of anthocyanins. Hundreds of anthocyanins have been purified and their structures determined (Strack and Wray 1994). Besides the structures of anthocyanins, changes of vacuolar pH, intermolecular sta ...
... Modification of the anthocyanidins mainly with glycosylation and acylation results in a wide variety of anthocyanins. Hundreds of anthocyanins have been purified and their structures determined (Strack and Wray 1994). Besides the structures of anthocyanins, changes of vacuolar pH, intermolecular sta ...
Chapter 6 Pichia pastoris
... the low frequency of multiple gene insertion events, the rate of transformation is between 1 and 10%, and is detected in Zeocin resistant transformants (Invitrogen Corporation, 2001). Multi-copy events can occur as gene insertions either at the AOX1 or the aox1: ARG4 loci ...
... the low frequency of multiple gene insertion events, the rate of transformation is between 1 and 10%, and is detected in Zeocin resistant transformants (Invitrogen Corporation, 2001). Multi-copy events can occur as gene insertions either at the AOX1 or the aox1: ARG4 loci ...
Analyses of complete nucleotide sequence of Iranian isolate of
... analyzed by t-test. There was a high correlation, often more than 0.9 between nucleotide identity values pertaining to individual genes and the complete genome. There was no significant difference between the two analyses in t-test (Table 5). Phylogenetic relationships among SCMVsubgroup viruses wer ...
... analyzed by t-test. There was a high correlation, often more than 0.9 between nucleotide identity values pertaining to individual genes and the complete genome. There was no significant difference between the two analyses in t-test (Table 5). Phylogenetic relationships among SCMVsubgroup viruses wer ...
Identification of proteins that putatively bind the
... The sequence of bHLH proteins is variable outside the bHLH domain, but members with similar features can be grouped into subfamilies (Pires and Dolan, 2009). SPCH, MUTE, and FAMA belong to the Ia subfamily. In addition to the bHLH domain, these three proteins share a conserved C-terminal sequence co ...
... The sequence of bHLH proteins is variable outside the bHLH domain, but members with similar features can be grouped into subfamilies (Pires and Dolan, 2009). SPCH, MUTE, and FAMA belong to the Ia subfamily. In addition to the bHLH domain, these three proteins share a conserved C-terminal sequence co ...
A-level Human Biology Question Paper Unit 04 - Bodies and
... Wrapping a person in blankets leads to an immediate warming of the skin. This stimulates thermoreceptors in the skin. This stimulation of thermoreceptors in the skin causes the fall in core body temperature seen in the first ten minutes. Suggest how. ...
... Wrapping a person in blankets leads to an immediate warming of the skin. This stimulates thermoreceptors in the skin. This stimulation of thermoreceptors in the skin causes the fall in core body temperature seen in the first ten minutes. Suggest how. ...
Anaerobic degradation of aromatic amino acids by
... and aromatic amino acids (the exceptions were valine, methionine, asparagine, aspartate and histidine) as a sole carbon and energy source. To the best of our knowledge, F. placidus is the first organism found to grow via anaerobic respiration with such a wide range of amino acids as the sole electro ...
... and aromatic amino acids (the exceptions were valine, methionine, asparagine, aspartate and histidine) as a sole carbon and energy source. To the best of our knowledge, F. placidus is the first organism found to grow via anaerobic respiration with such a wide range of amino acids as the sole electro ...
Markers of proliferation and differentiation in human keratinocyte
... Analysis of tissue response Data from keratinocyte monolayers are transposed to RHE : • The role of signaling intermediates and • The activation of particular (stressresponse) signaling pathways are currently under investigation See the work of L.-M. Koeper ...
... Analysis of tissue response Data from keratinocyte monolayers are transposed to RHE : • The role of signaling intermediates and • The activation of particular (stressresponse) signaling pathways are currently under investigation See the work of L.-M. Koeper ...
Two Genes with Similarity to Bacterial Response Regulators Are
... that predicted from our sequence. The predicted IBC7 protein is 72% identical and 91% similar to IBC6 across the response regulator domain (Figure 1C). In addition to the putative response regulator domain, the predicted IBC7 amino acid sequence contains a 10-kD C-terminal extension that has a Ser/P ...
... that predicted from our sequence. The predicted IBC7 protein is 72% identical and 91% similar to IBC6 across the response regulator domain (Figure 1C). In addition to the putative response regulator domain, the predicted IBC7 amino acid sequence contains a 10-kD C-terminal extension that has a Ser/P ...
109 GENES OF ARABIDOPSIS THALIANA INVOLVED IN WAX
... development, as well as in the inflorescence [8]. CER2 encodes a CoA-dependent acyltransferase, a component of the fatty acid elongase complex, apparently located in the nucleus [37; 14]. The cer2 mutant shows reduced levels of the decarbonylation pathway products and it accumulates C26 and C28 acyl ...
... development, as well as in the inflorescence [8]. CER2 encodes a CoA-dependent acyltransferase, a component of the fatty acid elongase complex, apparently located in the nucleus [37; 14]. The cer2 mutant shows reduced levels of the decarbonylation pathway products and it accumulates C26 and C28 acyl ...
... of RNA indicated liver to be the major site of at-M/HI-30 mRNA expression with lower levels observed in the stomach. The results suggest that modulation of at-M/HI-30 geue expression could play a role during porcine growth. Increased laT! L-chain mRNA levels may be particularly important in fetal an ...
A tale of two functions: enzymatic activity and
... assay with a CT b-enhanced yellow fluorescent protein (eYFP) fusion protein as the donor and the RNA binding dye Sytox Orange (Molecular Probes) as the acceptor. The gene for eYFP was amplified with primers eYFP5 and eYFP3, digested with XhoI and PacI, and cloned into equivalently digested pGDM9 and p ...
... assay with a CT b-enhanced yellow fluorescent protein (eYFP) fusion protein as the donor and the RNA binding dye Sytox Orange (Molecular Probes) as the acceptor. The gene for eYFP was amplified with primers eYFP5 and eYFP3, digested with XhoI and PacI, and cloned into equivalently digested pGDM9 and p ...
and paralogue-specific functions of acyl-CoA
... acyl-CoAs to acyl-CoA-utilizing enzymes and modulate their regulatory functions [12]. The ACBP family comprises basal acyl-CoA-binding proteins containing only the ACBP domain and larger proteins that contain an N-terminal ACBD (acyl-CoAbinding domain) as well as other domains. The prototypical basa ...
... acyl-CoAs to acyl-CoA-utilizing enzymes and modulate their regulatory functions [12]. The ACBP family comprises basal acyl-CoA-binding proteins containing only the ACBP domain and larger proteins that contain an N-terminal ACBD (acyl-CoAbinding domain) as well as other domains. The prototypical basa ...
Rapid Publication - Journal of Clinical Investigation
... We report here the cloning and sequence analysis of fulllength cDNAs from an ADA-deficient cell line. Four apparently identical, though independently isolated, clones have been sequenced. In each, a G to A mutation at base number 302 of the coding sequence was found, which predicts a glutamine resid ...
... We report here the cloning and sequence analysis of fulllength cDNAs from an ADA-deficient cell line. Four apparently identical, though independently isolated, clones have been sequenced. In each, a G to A mutation at base number 302 of the coding sequence was found, which predicts a glutamine resid ...
workshop - Dr Amy Yasko
... Previous studies have suggested that lead exposure may be associated with increased risk of amyotrophic lateral sclerosis (ALS). Polymorphisms in the genes for -aminolevulinic acid dehydratase (ALAD) and the vitamin D receptor (VDR) may affect susceptibility to lead exposure. We used data from a cas ...
... Previous studies have suggested that lead exposure may be associated with increased risk of amyotrophic lateral sclerosis (ALS). Polymorphisms in the genes for -aminolevulinic acid dehydratase (ALAD) and the vitamin D receptor (VDR) may affect susceptibility to lead exposure. We used data from a cas ...
INO1 - of /home/sholmes/web
... • Periphery localization and increase in mRNA expression are concurrent, with the highest level of increase in mRNA expression occurring after periphery localization is complete • These genes remain at the periphery long after the cells are returned to repressing conditions and mRNA expression retur ...
... • Periphery localization and increase in mRNA expression are concurrent, with the highest level of increase in mRNA expression occurring after periphery localization is complete • These genes remain at the periphery long after the cells are returned to repressing conditions and mRNA expression retur ...
Plant cell walls as targets for biotechnology
... polysaccharides have been purified and their cDNAs have been cloned but we have only recently begun to identify the genes responsible for polysaccharide synthesis. A major milestone just passed was the identification of a gene encoding a catalytic subunit of cellulose synthase [1••] and proof of its ...
... polysaccharides have been purified and their cDNAs have been cloned but we have only recently begun to identify the genes responsible for polysaccharide synthesis. A major milestone just passed was the identification of a gene encoding a catalytic subunit of cellulose synthase [1••] and proof of its ...
Polyclonal Anti-Collagen Type I
... glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69 ...
... glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69 ...
Additional Methods_Office2004
... both TIR and reflected fluorescence. All imaging was performed using a Nikon 100X 1.49 NA Apo TIRF oil immersion objective and collected over a 40.96 μm × 40.96 μm area onto an Andor iXon 897 EMCCD camera (Andor Technology, UK), with a frame size of 256 × 256 pixels and a corresponding pixel size of ...
... both TIR and reflected fluorescence. All imaging was performed using a Nikon 100X 1.49 NA Apo TIRF oil immersion objective and collected over a 40.96 μm × 40.96 μm area onto an Andor iXon 897 EMCCD camera (Andor Technology, UK), with a frame size of 256 × 256 pixels and a corresponding pixel size of ...
Journal of Steroid Biochemistry and Molecular Biology Cloning of
... 11-Hydroxysteroid dehydrogenase type 1 (11HSD1) is an enzyme that interconverts active 11hydroxy glucocorticoids (cortisol, corticosterone) and their inactive 11-oxo derivatives (cortisone, 11-dehydrocorticosterone). Although bidirectional, it is considered to operate in vivo as an 11-reductase tha ...
... 11-Hydroxysteroid dehydrogenase type 1 (11HSD1) is an enzyme that interconverts active 11hydroxy glucocorticoids (cortisol, corticosterone) and their inactive 11-oxo derivatives (cortisone, 11-dehydrocorticosterone). Although bidirectional, it is considered to operate in vivo as an 11-reductase tha ...
Roseobacter gallaeciensis sp. nov., a new marine - HAL
... BSc1, BSc4 and BSc20 were isolated from the surface of collectors used to allow scallop larval settlement (Instituto Oceanografico). All strains were selected on marine agar 2216 (MA; Difco). Subcultures of Roseobacter aµgicola, Roseobacter denitrificans and Roseobacter litoralis derived from ATCC s ...
... BSc1, BSc4 and BSc20 were isolated from the surface of collectors used to allow scallop larval settlement (Instituto Oceanografico). All strains were selected on marine agar 2216 (MA; Difco). Subcultures of Roseobacter aµgicola, Roseobacter denitrificans and Roseobacter litoralis derived from ATCC s ...
Paper 1
... To sustain energy and precursors for RNA biosynthesis, the ATP concentration in cells is generally maintained above 1 mM (71) with a high ATP/ADP.Pi ratio (72), so that there is sufficient free energy available to drive endergonic reactions. There are limits to the maintenance of these levels, below ...
... To sustain energy and precursors for RNA biosynthesis, the ATP concentration in cells is generally maintained above 1 mM (71) with a high ATP/ADP.Pi ratio (72), so that there is sufficient free energy available to drive endergonic reactions. There are limits to the maintenance of these levels, below ...
Nutrition acquisition strategies during fungal infection of plants
... Nitrogen constitutes a smaller part of the storage compounds in the spore and might represent a rate-limiting factor during penetration. Nevertheless, elevated expression of a di/ tri-peptide transporter (Ptr2) at the prepenetration stage in Stagonospora nodorum is not essential for pathogenicity (S ...
... Nitrogen constitutes a smaller part of the storage compounds in the spore and might represent a rate-limiting factor during penetration. Nevertheless, elevated expression of a di/ tri-peptide transporter (Ptr2) at the prepenetration stage in Stagonospora nodorum is not essential for pathogenicity (S ...
the genetic and cytogenetic localization of the three structural genes
... Stocks: The Drosophila strain Oregon-R was used as the reference wild-type strain in this work and, unless indicated otherwise, the LSP-1 of all stocks was the same as Oregon-R. Stocks were maintained on standard yeast-cornmeal-agar medium in half-pint milk bottles at 25". The other wild-type stocks ...
... Stocks: The Drosophila strain Oregon-R was used as the reference wild-type strain in this work and, unless indicated otherwise, the LSP-1 of all stocks was the same as Oregon-R. Stocks were maintained on standard yeast-cornmeal-agar medium in half-pint milk bottles at 25". The other wild-type stocks ...
LS1a Problem Set #2
... be bound to a MAPKK and nothing else, etc). Kinases are sequestered by scaffold proteins that are not fully occupied by kinases, and as a result, they are unable to participate in the signaling cascade. As the scaffolds each have multiple binding sites, you can treat them each individually for their ...
... be bound to a MAPKK and nothing else, etc). Kinases are sequestered by scaffold proteins that are not fully occupied by kinases, and as a result, they are unable to participate in the signaling cascade. As the scaffolds each have multiple binding sites, you can treat them each individually for their ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.