HIS-Select Nickel Affinity Gel (P6611) - Technical - Sigma
... The HIS-Select Nickel Affinity Gel is stored in 30% ethanol. The ethanol must be removed just prior to use. Thoroughly resuspend the affinity gel with gentle inversion and remove an appropriate aliquot for use. Take only the amount of affinity gel that is necessary for the purification to be done. T ...
... The HIS-Select Nickel Affinity Gel is stored in 30% ethanol. The ethanol must be removed just prior to use. Thoroughly resuspend the affinity gel with gentle inversion and remove an appropriate aliquot for use. Take only the amount of affinity gel that is necessary for the purification to be done. T ...
Write on zinc fingers
... The most straightforward method to generate new zinc finger arrays is to combine smaller zinc finger "modules" of known specificity. The structure of the zinc finger protein Zif268 bound to DNA described by Pavletich and Pabo in their 1991 publication has been key to much of this work and describes ...
... The most straightforward method to generate new zinc finger arrays is to combine smaller zinc finger "modules" of known specificity. The structure of the zinc finger protein Zif268 bound to DNA described by Pavletich and Pabo in their 1991 publication has been key to much of this work and describes ...
CHEMICAL SYNTHESIS OF PROTEINS : A TOOL FOR PROTEIN
... An ingenious stratagem useful to understand and modulate the structural and functional features of the proteins refers to the modification of their chemical structure. In this regard, the chemical synthesis of proteins appears a key tool, as it allows the unlimited modification of a polypeptide chai ...
... An ingenious stratagem useful to understand and modulate the structural and functional features of the proteins refers to the modification of their chemical structure. In this regard, the chemical synthesis of proteins appears a key tool, as it allows the unlimited modification of a polypeptide chai ...
View/Open - VUW research archive - Victoria University of Wellington
... having a second lab family. I would also like to acknowledge the fellow students, past and present, who I have come to know, in particular, Ploi Yibmantasiri, Janice Cheng, Nathaniel Dasaym, Bede Busby, Peter Birchirm, Katie Zeier, and Dr Christine Stockholm. Thank you for ...
... having a second lab family. I would also like to acknowledge the fellow students, past and present, who I have come to know, in particular, Ploi Yibmantasiri, Janice Cheng, Nathaniel Dasaym, Bede Busby, Peter Birchirm, Katie Zeier, and Dr Christine Stockholm. Thank you for ...
Starting the protein synthesis machine: eukaryotic
... unwind RNA secondary structure in the cap-proximal region of the mRNA. It does so much more efficiently as part of the eIF4F complex and aided by eIF4B, although the two proteins probably do not interact directly. eIF4A has a dumbbell structure consisting of two domains connected by a flexible linke ...
... unwind RNA secondary structure in the cap-proximal region of the mRNA. It does so much more efficiently as part of the eIF4F complex and aided by eIF4B, although the two proteins probably do not interact directly. eIF4A has a dumbbell structure consisting of two domains connected by a flexible linke ...
The Early Interaction of the Outer Membrane Protein PhoE with
... material) on SDS-polyacrylamide gel electrophoresis (PhoE) or immunoprecipitated (OmpA). The release values for PhoE presented in the text are an average of at least three experiments. Pulse-chase Labeling—Cells were grown exactly as described above and pulsed for 30 s with [35S]methionine (30 Ci p ...
... material) on SDS-polyacrylamide gel electrophoresis (PhoE) or immunoprecipitated (OmpA). The release values for PhoE presented in the text are an average of at least three experiments. Pulse-chase Labeling—Cells were grown exactly as described above and pulsed for 30 s with [35S]methionine (30 Ci p ...
The SMN Complex Is Associated with snRNPs throughout Their
... The common neurodegenerative disease spinal muscular atrophy is caused by reduced levels of the survival of motor neurons (SMN) protein. SMN associates with several proteins (Gemin2 to Gemin6) to form a large complex which is found both in the cytoplasm and in the nucleus. The SMN complex functions ...
... The common neurodegenerative disease spinal muscular atrophy is caused by reduced levels of the survival of motor neurons (SMN) protein. SMN associates with several proteins (Gemin2 to Gemin6) to form a large complex which is found both in the cytoplasm and in the nucleus. The SMN complex functions ...
A novel exon within the mdm2 gene modulates translation
... is predicted to initiate translation at AUG62 (Figure 3c). When translated in vitro, the truncated mutant comigrated with mdm2a suggesting that the N-terminus of the putative mdm2a protein is equivalent to AUG62 of mdm2 (Figure 3a, lanes 3 and 4). Mdm2a translation in vitro occurs through a re-initi ...
... is predicted to initiate translation at AUG62 (Figure 3c). When translated in vitro, the truncated mutant comigrated with mdm2a suggesting that the N-terminus of the putative mdm2a protein is equivalent to AUG62 of mdm2 (Figure 3a, lanes 3 and 4). Mdm2a translation in vitro occurs through a re-initi ...
Dual-topology membrane proteins Escherichia coli Susanna Seppälä
... Cellular identity relies on the existence of the cellular membrane, a semipermeable barrier that encloses any cell and defines its boundary. In many cells, the interior is further divided into membrane enclosed compartments with specialized functions (organelles), and multicellular organisms are, si ...
... Cellular identity relies on the existence of the cellular membrane, a semipermeable barrier that encloses any cell and defines its boundary. In many cells, the interior is further divided into membrane enclosed compartments with specialized functions (organelles), and multicellular organisms are, si ...
Solubility of recombinant Src homology 2 domains expressed in E
... by TANGO, we generated TFV → GYT and FV → YT mutants of the TSAd-90-188-PHRD and TSAd-90-188-PAAS constructs, and compared their expression to that of the ALX SH2 domain. These constructs were chosen, as they contained the shortest flanking sequences compared to the original TSAd 1-TD construct, and ...
... by TANGO, we generated TFV → GYT and FV → YT mutants of the TSAd-90-188-PHRD and TSAd-90-188-PAAS constructs, and compared their expression to that of the ALX SH2 domain. These constructs were chosen, as they contained the shortest flanking sequences compared to the original TSAd 1-TD construct, and ...
Understanding the acetylome: translating targeted
... versible acetylation (1, 32, 63, 76). These modifications also appeared functionally relevant, as an enrichment of acetylated histones was observed in transcribed DNA sequences (62). Acetylation results in neutralization of lysine residues located in amino-terminal domains of histones (62). Function ...
... versible acetylation (1, 32, 63, 76). These modifications also appeared functionally relevant, as an enrichment of acetylated histones was observed in transcribed DNA sequences (62). Acetylation results in neutralization of lysine residues located in amino-terminal domains of histones (62). Function ...
Diacylglycerol kinases - University of Toronto Mississauga
... these domains may bind DAG, perhaps localizing DGKs to where DAG accumulates. However, no DGK C1 domain has so far been conclusively shown to bind DAG. In fact, structural predictions suggest that most DGK C1 domains may not bind DAG. For example, Hurley et al. [26] noted that the amino acid sequenc ...
... these domains may bind DAG, perhaps localizing DGKs to where DAG accumulates. However, no DGK C1 domain has so far been conclusively shown to bind DAG. In fact, structural predictions suggest that most DGK C1 domains may not bind DAG. For example, Hurley et al. [26] noted that the amino acid sequenc ...
Replication-Coupled Packaging Mechanism in Positive
... Expression and biological activity of FHV agrotransformants. The agroinfiltration system has been used successfully for the DNA-based expression of biologically active RNA components of BMV in N. benthamiana (6, 25). To extend this system to FHV, N. benthamiana leaves were infiltrated with Agrobacte ...
... Expression and biological activity of FHV agrotransformants. The agroinfiltration system has been used successfully for the DNA-based expression of biologically active RNA components of BMV in N. benthamiana (6, 25). To extend this system to FHV, N. benthamiana leaves were infiltrated with Agrobacte ...
Histones - scientia.global
... Histone mRNAs are synthesised in a distinct subcompartment of the nucleus, termed the histone locus body (HLB), that concentrates many of the factors that are required for histone mRNA biosynthesis. As mentioned above, the protein FLASH and U7 snRNP are components of the HLB that participate in 3′ p ...
... Histone mRNAs are synthesised in a distinct subcompartment of the nucleus, termed the histone locus body (HLB), that concentrates many of the factors that are required for histone mRNA biosynthesis. As mentioned above, the protein FLASH and U7 snRNP are components of the HLB that participate in 3′ p ...
Reactive cysteine in proteins: Protein folding - Genoma
... Besides glutathione, thiol proteins such as thioredoxin, glutaredoxin (also known as thioltransferase) and protein disulfide isomerase are also involved in the regulation of the intracellular redox balance and, therefore, they are also known as thiol/disulfide oxido-reductases. Thioredoxin appears t ...
... Besides glutathione, thiol proteins such as thioredoxin, glutaredoxin (also known as thioltransferase) and protein disulfide isomerase are also involved in the regulation of the intracellular redox balance and, therefore, they are also known as thiol/disulfide oxido-reductases. Thioredoxin appears t ...
Cellular functions of the BRCA tumour
... two highly conserved domains located in the N- and Cterminal regions of the protein, which include a RING domain located in the N-terminus and two tandem BRCT motifs at the extreme C-terminal end (Figure 1). The N-terminal ...
... two highly conserved domains located in the N- and Cterminal regions of the protein, which include a RING domain located in the N-terminus and two tandem BRCT motifs at the extreme C-terminal end (Figure 1). The N-terminal ...
Plant/Fungi Total RNA Purification 96-Well Kit
... Norgen’s Plant/Fungi Total RNA Purification 96-Well Kit provides a rapid method for the highthroughput isolation of total RNA from plants and fungi. The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the ...
... Norgen’s Plant/Fungi Total RNA Purification 96-Well Kit provides a rapid method for the highthroughput isolation of total RNA from plants and fungi. The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the ...
Report Organelles in Blastocystis that Blur the
... of the multienzyme PDH complex (Figure 3 and Table S2). Thus, Blastocystis has two ways to decarboxylate pyruvate to form acetyl-CoA: one involves the classic mitochondrial PDH complex and the other involves the anaerobic PFO (Figure 3). Interestingly, although no PFO has been found yet in Nyctother ...
... of the multienzyme PDH complex (Figure 3 and Table S2). Thus, Blastocystis has two ways to decarboxylate pyruvate to form acetyl-CoA: one involves the classic mitochondrial PDH complex and the other involves the anaerobic PFO (Figure 3). Interestingly, although no PFO has been found yet in Nyctother ...
Small aminoacyl transfer centers at GU within a larger RNA
... Thus, though total aminoacylation slows thirty-fold with increased loop constraint from surrounding sequences, activity remains detectable. In fact, aminoacylated product accumulation appears to be a sensitive monitor of structure at the site of the ribozyme, perhaps usable for this purpose in other ...
... Thus, though total aminoacylation slows thirty-fold with increased loop constraint from surrounding sequences, activity remains detectable. In fact, aminoacylated product accumulation appears to be a sensitive monitor of structure at the site of the ribozyme, perhaps usable for this purpose in other ...
Two Microtubule-Associated Proteins of the
... negative staining indicated that microtubules were crisscrossed with each other, and very little if any bundling phenomenon was observed (Fig. 2D). These individual microtubules were brought together by the AtMAP65-6-induced crisscrosses to form a dense mesh-like network (Fig. 2C). To test whether s ...
... negative staining indicated that microtubules were crisscrossed with each other, and very little if any bundling phenomenon was observed (Fig. 2D). These individual microtubules were brought together by the AtMAP65-6-induced crisscrosses to form a dense mesh-like network (Fig. 2C). To test whether s ...
Molecular insights into RNA and DNA helicase evolution from the
... RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a three-dimensional structure that is held in shape by regions of the molecule interacting with each other. Before DNA and RNA can perform their essential tasks in cells, enzymes called helicases must s ...
... RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a three-dimensional structure that is held in shape by regions of the molecule interacting with each other. Before DNA and RNA can perform their essential tasks in cells, enzymes called helicases must s ...
YangSpr07
... RGD peptides are short peptide fragments derived from the amino acid sequence of several extracellular matrix proteins, such as fibrinogen, fibronectin, vitronectin, collagen, and laminin. The amino acid sequence Arg-Gly-Asp or RGD present on extracellular matrix proteins is known to be a requiremen ...
... RGD peptides are short peptide fragments derived from the amino acid sequence of several extracellular matrix proteins, such as fibrinogen, fibronectin, vitronectin, collagen, and laminin. The amino acid sequence Arg-Gly-Asp or RGD present on extracellular matrix proteins is known to be a requiremen ...
Refining the Definition of Plant Mitochondrial
... III subunits; Table I). However, in both rice and Arabidopsis, a notable exception was Glu dehydrogenase (GDH), which had an N-terminal acetylated intact sequence (Table I; Supplemental Fig. S1). Specific peptides from other regions of the different GDH isoforms listed in Table I, from gel protein s ...
... III subunits; Table I). However, in both rice and Arabidopsis, a notable exception was Glu dehydrogenase (GDH), which had an N-terminal acetylated intact sequence (Table I; Supplemental Fig. S1). Specific peptides from other regions of the different GDH isoforms listed in Table I, from gel protein s ...
Structural Mechanisms for Regulation of Membrane
... As indicated in Figure 1A, Rab proteins possess a characteristic GTPase fold with two ‘switch regions’ that contact the γ phosphate of GTP and exhibit large conformational differences between the inactive and active states (11,12). An essential Mg2+ cofactor is required for high affinity nucleotide ...
... As indicated in Figure 1A, Rab proteins possess a characteristic GTPase fold with two ‘switch regions’ that contact the γ phosphate of GTP and exhibit large conformational differences between the inactive and active states (11,12). An essential Mg2+ cofactor is required for high affinity nucleotide ...
Proteins of Human Milk. I. Identification of Major Components
... M ilk secretion. T he fat globules of milk bud off breast exocri ne cells and are covered wit h authentic cell membrane, which may be isolated a nd analyzed (3 , 24-36). Specific surface- differe nt iation an tigens of human mammary epithelial cells have been isolated from membranes of fat globules ...
... M ilk secretion. T he fat globules of milk bud off breast exocri ne cells and are covered wit h authentic cell membrane, which may be isolated a nd analyzed (3 , 24-36). Specific surface- differe nt iation an tigens of human mammary epithelial cells have been isolated from membranes of fat globules ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.