Glycoprotein IIIa Is Phosphorylated in Intact Human
... 1). The purified material was essentially homogeneous (Fig 1, lane 1). Only one component of the complex was phosphorylated. This was identified as G P IIIa (lanes 2 and 4) on the basis of its co-electrophoresis with iodinated G P IIIa (lanes 3 and 5) from the purified G P IIb-IIIa complex. The poss ...
... 1). The purified material was essentially homogeneous (Fig 1, lane 1). Only one component of the complex was phosphorylated. This was identified as G P IIIa (lanes 2 and 4) on the basis of its co-electrophoresis with iodinated G P IIIa (lanes 3 and 5) from the purified G P IIb-IIIa complex. The poss ...
Termination of translation: interplay of mRNA, rRNAs and release
... by the fact that the antibiotic neomycin, which binds to conserved sequences of 16S rRNA (Moazed and Noller, 1987) and induces high levels of error in translation of sense codons by tRNAs, does not make bacterial RFs more error prone (Freistroffer et al., 2000). ...
... by the fact that the antibiotic neomycin, which binds to conserved sequences of 16S rRNA (Moazed and Noller, 1987) and induces high levels of error in translation of sense codons by tRNAs, does not make bacterial RFs more error prone (Freistroffer et al., 2000). ...
Loss of the Mili-interacting Tudor domain–containing protein
... kidney (HEK) 293T cell cultures. Anti-HA Figure 1 Tdrd1 is a Mili-specific interacting factor. (a) Above, known protein domains of Tdrd1 are immunoprecipitation and western blot ana- shown. Middle, Tdrd1 interacts with Mili, but not Miwi, in adult mouse testes extracts. Reciprocally, lysis with the ...
... kidney (HEK) 293T cell cultures. Anti-HA Figure 1 Tdrd1 is a Mili-specific interacting factor. (a) Above, known protein domains of Tdrd1 are immunoprecipitation and western blot ana- shown. Middle, Tdrd1 interacts with Mili, but not Miwi, in adult mouse testes extracts. Reciprocally, lysis with the ...
2nd_lecture
... between two cytochrome c sequences is proportional to the phylogenetic difference between the species from which they are derived • This observation can be used to build phylogenetic trees of proteins • This is the basis for studies of molecular ...
... between two cytochrome c sequences is proportional to the phylogenetic difference between the species from which they are derived • This observation can be used to build phylogenetic trees of proteins • This is the basis for studies of molecular ...
Stochastic and reversible aggregation of mRNA with expanded CUG
... To analyze the dynamics of the CUG-rich RNA foci in living cells, the lacZ–MS2–5CUG and lacZ–MS2–145CUG cell lines were infected with retroviral vector expressing MS2–GFP, and live-cell microscopy was performed at a rate of three images per second using a spinning disk confocal microscope, to visual ...
... To analyze the dynamics of the CUG-rich RNA foci in living cells, the lacZ–MS2–5CUG and lacZ–MS2–145CUG cell lines were infected with retroviral vector expressing MS2–GFP, and live-cell microscopy was performed at a rate of three images per second using a spinning disk confocal microscope, to visual ...
Splice switching efficiency and specificity for oligonucleotides
... The use of antisense oligonucleotides to modulate splicing patterns has gained increasing attention as a therapeutic platform and, hence, the mechanisms of splice switching oligonucleotides are of interest. Cells expressing luciferase pre-mRNA interrupted by an aberrantly spliced β-globin intron, He ...
... The use of antisense oligonucleotides to modulate splicing patterns has gained increasing attention as a therapeutic platform and, hence, the mechanisms of splice switching oligonucleotides are of interest. Cells expressing luciferase pre-mRNA interrupted by an aberrantly spliced β-globin intron, He ...
A chitin-binding domain in a marine bacterial
... To examine the ecology and evolution of microbial chitinases, especially the chitin-binding domain, one of the chitinase genes (&A) from the marine bacterium Vibrio harveyi was analysed. The deduced amino acid sequence of ChiA is not very similar overall to other proteins, except for two regions, th ...
... To examine the ecology and evolution of microbial chitinases, especially the chitin-binding domain, one of the chitinase genes (&A) from the marine bacterium Vibrio harveyi was analysed. The deduced amino acid sequence of ChiA is not very similar overall to other proteins, except for two regions, th ...
Case Study: BPTI
... pancreas. BPTI is a member of the serine protease family of inhibitors [1]. A hallmark of this class of enzymes are the many conserved cysteine residues that form disulfide bonds stabilizing the proteins’ three-dimensional structures. BPTI has a relatively broad specificity in that it can inhibit se ...
... pancreas. BPTI is a member of the serine protease family of inhibitors [1]. A hallmark of this class of enzymes are the many conserved cysteine residues that form disulfide bonds stabilizing the proteins’ three-dimensional structures. BPTI has a relatively broad specificity in that it can inhibit se ...
from dicp.ac.cn
... p38α can directly interact with the α and β subunits of CK2 to activate the holoenzyme (17). p38 and CK2 both co-immunoprecipitate with p53 (18, 19). Anisomycin and tumor necrosis factor-α (TNF-α)-induced phosphorylation of p53 at Ser392 requires p38 MAPK kinase and CK2 activities (17). In additiona ...
... p38α can directly interact with the α and β subunits of CK2 to activate the holoenzyme (17). p38 and CK2 both co-immunoprecipitate with p53 (18, 19). Anisomycin and tumor necrosis factor-α (TNF-α)-induced phosphorylation of p53 at Ser392 requires p38 MAPK kinase and CK2 activities (17). In additiona ...
The Foamy Virus Gag Proteins: What Makes Them Different?
... cleavage sites do not abrogate particle release, but lead to noninfectious viruses [14,20]. Interestingly, despite their close proximity, a mutation in one cannot be functionally substituted by the presence of the two other cleavage sites. This suggests that all three secondary cleavage sites have a ...
... cleavage sites do not abrogate particle release, but lead to noninfectious viruses [14,20]. Interestingly, despite their close proximity, a mutation in one cannot be functionally substituted by the presence of the two other cleavage sites. This suggests that all three secondary cleavage sites have a ...
The Ovine Lens Cytoskeleton - Lincoln University Research Archive
... Intermediate filament proteins are referred to as such on the basis of their average diameter (10 nm) is intermediate to that of microfilaments (characteristically 5-8 nm in diameter) and the microtubules (typically 20-25 nm in diameter; Strelkov et al., 2003; Pitz & Moll, 2002). Intermediate filame ...
... Intermediate filament proteins are referred to as such on the basis of their average diameter (10 nm) is intermediate to that of microfilaments (characteristically 5-8 nm in diameter) and the microtubules (typically 20-25 nm in diameter; Strelkov et al., 2003; Pitz & Moll, 2002). Intermediate filame ...
RNA helicase DDX19 stabilizes ribosomal elongation and
... presence of the characteristic eponymous sequence AspGlu-Ala-Asp (DEAD) (5). One representative of this family is the human ATP-dependent RNA-helicase DDX19 (3). It is an essential protein that is conserved in eukaryotes (6,7). Like in all members of the family, the helicase core of DDX19 consists o ...
... presence of the characteristic eponymous sequence AspGlu-Ala-Asp (DEAD) (5). One representative of this family is the human ATP-dependent RNA-helicase DDX19 (3). It is an essential protein that is conserved in eukaryotes (6,7). Like in all members of the family, the helicase core of DDX19 consists o ...
Molecular diversity through RNA editing: a balancing act
... properties of the intrinsically promiscuous RNA editing machinery paired with the characteristic Alu pair RNA secondary structure induces highly efficient multiple site editing in Alu elements. In fact, predictions based on the existing data posit that more than 98% of all pre-mRNAs are subject to A ...
... properties of the intrinsically promiscuous RNA editing machinery paired with the characteristic Alu pair RNA secondary structure induces highly efficient multiple site editing in Alu elements. In fact, predictions based on the existing data posit that more than 98% of all pre-mRNAs are subject to A ...
Protein stabilization: a common consequence of mutations
... Myc is overexpressed in many cancers as a result of gene rearrangement or ampli®cation, but coding sequence changes which cluster in the N-terminal transactivation domain also appear to play a role in tumour progression. The prototypic v-Myc gene of MC29 virus diers from avian c-Myc by a series of ...
... Myc is overexpressed in many cancers as a result of gene rearrangement or ampli®cation, but coding sequence changes which cluster in the N-terminal transactivation domain also appear to play a role in tumour progression. The prototypic v-Myc gene of MC29 virus diers from avian c-Myc by a series of ...
Vanadium-Binding Proteins (Vanabins)
... determine the ratio of vanadium atoms per protein molecule at various concentrations of free vanadium(IV)(VO2+) and vanadium(V)([VO4]3-) ions. We used vanadium(IV) ions coordinated by iminodiacetic acid (IDA), because free vanadium(IV) ions precipitate at neutral pH. IDA was chosen because it does ...
... determine the ratio of vanadium atoms per protein molecule at various concentrations of free vanadium(IV)(VO2+) and vanadium(V)([VO4]3-) ions. We used vanadium(IV) ions coordinated by iminodiacetic acid (IDA), because free vanadium(IV) ions precipitate at neutral pH. IDA was chosen because it does ...
Protein targeting, translocation and Escherichia coli Proteomic analysis of substrate-pathway relationships
... Single spanning α-helical membrane proteins have of one TM domain, while multispanning α-helical membrane proteins have of two or more TM domains connected together by hydrophilic loop regions. The loop regions can be short linkers consisting of just a few amino acids or they can consist of a long ...
... Single spanning α-helical membrane proteins have of one TM domain, while multispanning α-helical membrane proteins have of two or more TM domains connected together by hydrophilic loop regions. The loop regions can be short linkers consisting of just a few amino acids or they can consist of a long ...
Synthetic Physical Interactions Map Kinetochore
... strongest 156 interactions using the SPI methodology with 16 replicates per strain (Figure 1, C and D). This high-density retesting confirmed the strongest interactions (Figure S1C), and identified 37 GFP-tagged proteins that consistently produce a strong SPI phenotype with Mad2-GBP (Figure 1C, dashed ...
... strongest 156 interactions using the SPI methodology with 16 replicates per strain (Figure 1, C and D). This high-density retesting confirmed the strongest interactions (Figure S1C), and identified 37 GFP-tagged proteins that consistently produce a strong SPI phenotype with Mad2-GBP (Figure 1C, dashed ...
A proteomic study of African elephant milk: Inter
... which contains the base uracil instead of thiamine in DNA. The mRNA which is now the blueprint for the protein and determines the amino acid sequence of the protein it codes for moves to the cytosol and the endoplasmic reticulum (ER) where translation occurs to form a polypeptide with the aid of tRN ...
... which contains the base uracil instead of thiamine in DNA. The mRNA which is now the blueprint for the protein and determines the amino acid sequence of the protein it codes for moves to the cytosol and the endoplasmic reticulum (ER) where translation occurs to form a polypeptide with the aid of tRN ...
Structural studies on lysosomal proteins
... encoding lysosomal or lysosome related proteins result in over 50 different lysosomal storage disorders (LSDs) affecting 1 out of every 7700 newborn children. For instance, the first described LSD, Pompe disease, is caused by a mutation that impairs the function of lysosomal !-glucosidase (GAA) and ...
... encoding lysosomal or lysosome related proteins result in over 50 different lysosomal storage disorders (LSDs) affecting 1 out of every 7700 newborn children. For instance, the first described LSD, Pompe disease, is caused by a mutation that impairs the function of lysosomal !-glucosidase (GAA) and ...
pdf file - John Innes Centre
... and GlnK, and their homologs are commonly found in other bacteria (12). The conserved genetic linkage between glnK and amtB suggested a functional association between GlnK and AmtB (10), and indeed an increase in cellular nitrogen status leads to AmtB-GlnK association and consequent inactivation of ...
... and GlnK, and their homologs are commonly found in other bacteria (12). The conserved genetic linkage between glnK and amtB suggested a functional association between GlnK and AmtB (10), and indeed an increase in cellular nitrogen status leads to AmtB-GlnK association and consequent inactivation of ...
Lactococcus lactis as expression host for the biosynthetic
... quantum yield (Q) are highly sensitive parameters for monitoring changes in the structure and dynamics of a protein. In numerous studies, the intrinsic protein fluorescence was used to monitor processes like enzyme kinetics, protein–ligand interactions or protein (un)folding. Modern molecular biolog ...
... quantum yield (Q) are highly sensitive parameters for monitoring changes in the structure and dynamics of a protein. In numerous studies, the intrinsic protein fluorescence was used to monitor processes like enzyme kinetics, protein–ligand interactions or protein (un)folding. Modern molecular biolog ...
TRIzol Reagent
... For additional information on stopping points, see Focus 20:2 p. 36. Considerations for sample: Sample volume should NOT exceed 10% of the volume of the reagent. Use of NP40 or Triton in the sample: The sample may contain up to 1.0% NP40 or Triton X 100 without interfering with isolation. Scaling of ...
... For additional information on stopping points, see Focus 20:2 p. 36. Considerations for sample: Sample volume should NOT exceed 10% of the volume of the reagent. Use of NP40 or Triton in the sample: The sample may contain up to 1.0% NP40 or Triton X 100 without interfering with isolation. Scaling of ...
How do potentials derived from structural databases relate to true
... high linear correlation coefficient (ranging from 0.84 to 0.91) (Mimy & Shakhnovich, 1996). However, the repulsive terms in the potentials were systematically underestimated when compared with the attractive terms. The potentials also seemed to be overoptimized because the derived potentials gave an ...
... high linear correlation coefficient (ranging from 0.84 to 0.91) (Mimy & Shakhnovich, 1996). However, the repulsive terms in the potentials were systematically underestimated when compared with the attractive terms. The potentials also seemed to be overoptimized because the derived potentials gave an ...
PDF
... cortex of the oocyte, whereas the methylation of another subset controls germ cell migration during embryogenesis. These results demonstrate a crucial role of arginine methylation in directing the subcellular localisation of SmB and that this modification contributes specifically to the establishmen ...
... cortex of the oocyte, whereas the methylation of another subset controls germ cell migration during embryogenesis. These results demonstrate a crucial role of arginine methylation in directing the subcellular localisation of SmB and that this modification contributes specifically to the establishmen ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.