Correlating ribosome function with high
... Figure 1. The ribosome functional centers. (a) The two ribosomal subunits. Left: the small ribosomal subunit (T30S) [4]. The approximate positions of codon–anticodon interactions of A-, P- and E-tRNAs are shown and the main functional domains are indicated: H, head; L, latch; P, platform; S, shoulde ...
... Figure 1. The ribosome functional centers. (a) The two ribosomal subunits. Left: the small ribosomal subunit (T30S) [4]. The approximate positions of codon–anticodon interactions of A-, P- and E-tRNAs are shown and the main functional domains are indicated: H, head; L, latch; P, platform; S, shoulde ...
RNA polyadenylation and decay in mitochondria and chloroplasts.
... mRNA (excluding histone mRNAs) is a well‐defined and long‐known phenomenon in eukaryotes (Fig. 1) (6). Historically, the observations that mRNA was mostly retained on an oligo(dT) column, and that cDNA could be obtained by reverse transcription of total RNA using an oligo(dT) primer, paved the way f ...
... mRNA (excluding histone mRNAs) is a well‐defined and long‐known phenomenon in eukaryotes (Fig. 1) (6). Historically, the observations that mRNA was mostly retained on an oligo(dT) column, and that cDNA could be obtained by reverse transcription of total RNA using an oligo(dT) primer, paved the way f ...
Chemistry 1010
... – 9000 different proteins in a cell – Individual human being >100,000 different – Fibrous Protein • Insoluble in H2O • Used mainly for structural purposes ...
... – 9000 different proteins in a cell – Individual human being >100,000 different – Fibrous Protein • Insoluble in H2O • Used mainly for structural purposes ...
RNA Synthesis and Degradation during Antibiotic
... washed and resuspended in antibiotic-free medium from which uracil, thymine, glucose, ammonium chloride or phosphate was omitted. Ordinarily, organisms treated as above and resuspended in complete medium began to divide only after incubation for 45 min. The requirement for various nutrients during t ...
... washed and resuspended in antibiotic-free medium from which uracil, thymine, glucose, ammonium chloride or phosphate was omitted. Ordinarily, organisms treated as above and resuspended in complete medium began to divide only after incubation for 45 min. The requirement for various nutrients during t ...
Structural insights into the coupling of virion assembly and rotavirus
... proteins effectively neutralize rotaviruses. Thus, individual rotavirus strains are often classified and described by a binary serotype system (GxP[y])18 (BOX 2) analogous to the HxNy designation of influenza virus strains. A comparison of reovirus and rotavirus virions, as representatives of the tu ...
... proteins effectively neutralize rotaviruses. Thus, individual rotavirus strains are often classified and described by a binary serotype system (GxP[y])18 (BOX 2) analogous to the HxNy designation of influenza virus strains. A comparison of reovirus and rotavirus virions, as representatives of the tu ...
Descriptions of Banbury Conference
... The highly productive discussions focused attention on the role of FMRP as a mRNA binding protein and upon the likelihood that the phenotype of FXS reflects where and if the mRNAs that FMRP are translated within the neuron (or other cell). Of particular interest were the first reports, independently ...
... The highly productive discussions focused attention on the role of FMRP as a mRNA binding protein and upon the likelihood that the phenotype of FXS reflects where and if the mRNAs that FMRP are translated within the neuron (or other cell). Of particular interest were the first reports, independently ...
Anterior PAR proteins function during cytokinesis and
... the four-cell stage. Localization of PAR-6-mCherry and PAR-2-GFP are temporally and spatially localized along the cleavage furrow membrane. PAR-6 and PAR-2 localize to the furrow membrane during ingression but are distinct in that PAR-2-GFP is excluded from the extreme tip of the furrow (white arrow ...
... the four-cell stage. Localization of PAR-6-mCherry and PAR-2-GFP are temporally and spatially localized along the cleavage furrow membrane. PAR-6 and PAR-2 localize to the furrow membrane during ingression but are distinct in that PAR-2-GFP is excluded from the extreme tip of the furrow (white arrow ...
Protein sequence databases
... when the first protein sequence database was published as printed version, Margaret Dayhoff defined the basic principles of this discipline with only a small number of sequences at hand. Nowadays, with more than a million protein sequences available in public databases, a constantly growing number o ...
... when the first protein sequence database was published as printed version, Margaret Dayhoff defined the basic principles of this discipline with only a small number of sequences at hand. Nowadays, with more than a million protein sequences available in public databases, a constantly growing number o ...
Effect of the addition of CMC on the aggregation behaviour
... Mw shift toward higher values. Also, SPI contains more tyrosine residues than WPC, 3.3% vs. 2.5%, respectively (Stuchell and Krochta, 1994). Moreover, CMC seemed to interact with the protein mixture in ...
... Mw shift toward higher values. Also, SPI contains more tyrosine residues than WPC, 3.3% vs. 2.5%, respectively (Stuchell and Krochta, 1994). Moreover, CMC seemed to interact with the protein mixture in ...
Sample pages 1 PDF
... nematodes. As early as in fully-developed J2 in the egg, the extensions of the subventral glands of root-knot and cyst nematodes are packed with secretory granules whereas the dorsal gland extension is relatively empty. During the transition from host-root penetration to feeding site induction and m ...
... nematodes. As early as in fully-developed J2 in the egg, the extensions of the subventral glands of root-knot and cyst nematodes are packed with secretory granules whereas the dorsal gland extension is relatively empty. During the transition from host-root penetration to feeding site induction and m ...
The Importance of Cell Motility
... describing the complex equilibria. Dr. Mechthild Schroeter continues to work along with Dr. Gabriele Pfitzer's laboratory, on the caldesmon-myosin interaction in knock-out mice. Unless one is aware of these possibilities when designing experiments one may be misled. Under some conditions caldesmon i ...
... describing the complex equilibria. Dr. Mechthild Schroeter continues to work along with Dr. Gabriele Pfitzer's laboratory, on the caldesmon-myosin interaction in knock-out mice. Unless one is aware of these possibilities when designing experiments one may be misled. Under some conditions caldesmon i ...
Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets Christian Hentrich
... work has shown that it is possible to form phospholipid vesicles that contain dextran/PEG ATPSs (Helfrich et al. 2002; Long et al. 2005; Dominak et al. 2010), and that these systems are able to partition RNA to sub-regions within a vesicle. We were able to encapsulate a dextran/ PEG ATPS inside olei ...
... work has shown that it is possible to form phospholipid vesicles that contain dextran/PEG ATPSs (Helfrich et al. 2002; Long et al. 2005; Dominak et al. 2010), and that these systems are able to partition RNA to sub-regions within a vesicle. We were able to encapsulate a dextran/ PEG ATPS inside olei ...
Coordination of Genomic RNA Packaging with Viral Assembly in HIV-1
... A similar observation was made in Rous sarcoma virus (RSV) where the NC domain was demonstrated to be essential for cytoplasmic oligomerization [10]. The assembly of oligomers through NC does not require specific interactions with viral RNA, since replacement of the domain with dimerizing leucine zi ...
... A similar observation was made in Rous sarcoma virus (RSV) where the NC domain was demonstrated to be essential for cytoplasmic oligomerization [10]. The assembly of oligomers through NC does not require specific interactions with viral RNA, since replacement of the domain with dimerizing leucine zi ...
Lectins as PIant Defense Proteins
... TYPE 2 RIPS: A SPECIAL CLASS OF CHIMEROLECTINS WlTH A GENERAL TOXlClTY TOWARD ALL ...
... TYPE 2 RIPS: A SPECIAL CLASS OF CHIMEROLECTINS WlTH A GENERAL TOXlClTY TOWARD ALL ...
C urrent and prospective applications of metal ion–protein
... the main targets for hard metal ions such as Fe(III) and Mg(II). A well-accepted concept is that the spatial distribution of histidine residues over a protein surface and their accessibility would influence the retention behavior of the protein molecule [33]. Several high metal affinity peptides hav ...
... the main targets for hard metal ions such as Fe(III) and Mg(II). A well-accepted concept is that the spatial distribution of histidine residues over a protein surface and their accessibility would influence the retention behavior of the protein molecule [33]. Several high metal affinity peptides hav ...
Site-selective incorporation and ligation of
... oxidised under certain conditions (cysteine and methionine residues in particular).23 Therefore, the reaction is usually performed with or quenched by another component such as excess methionine or ethylene glycol, followed by purification of the oxidised protein,25,26 which exists in an equilibrium ...
... oxidised under certain conditions (cysteine and methionine residues in particular).23 Therefore, the reaction is usually performed with or quenched by another component such as excess methionine or ethylene glycol, followed by purification of the oxidised protein,25,26 which exists in an equilibrium ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... the rough endoplasmic reticulum (RER) are sorted and delivered to their respective vacuoles. Thus, different targeting machineries for each type of vacuole must be involved in the protein transport in these cells. Kirsch et al. (1994) demonstrated that the clathrin-coated vesicles contain a potentia ...
... the rough endoplasmic reticulum (RER) are sorted and delivered to their respective vacuoles. Thus, different targeting machineries for each type of vacuole must be involved in the protein transport in these cells. Kirsch et al. (1994) demonstrated that the clathrin-coated vesicles contain a potentia ...
Abiotic stress in plants: Late Embryogenesis Abundant proteins Imen Amara
... survival or death of the affected plants (Ahuja I 2010; Xiong et al. 2002; Yamaguchi-Shinozaki and Shinozaki 2006). In Arabidopsis gene networks of stress-inducible genes have been identified and have been classified into two groups: regulatory and functional genes (Cramer et al. 2011; Shinozaki and ...
... survival or death of the affected plants (Ahuja I 2010; Xiong et al. 2002; Yamaguchi-Shinozaki and Shinozaki 2006). In Arabidopsis gene networks of stress-inducible genes have been identified and have been classified into two groups: regulatory and functional genes (Cramer et al. 2011; Shinozaki and ...
Mechanistic Studies Of Drug Resistance Conferred By An ABC
... Fig. 1.1 Schematic representation of the conserved motifs in DrrA…………………………….43 Fig. 1.2 Disulfide cross-linking between S319C in DrrA and S23C or C260 in DrrB………….45 Fig. 1.3 Disulfide cross-linking between T311C, S302C, S287C, S253C, or S232C in DrrA and S23C in DrrB… ............................. ...
... Fig. 1.1 Schematic representation of the conserved motifs in DrrA…………………………….43 Fig. 1.2 Disulfide cross-linking between S319C in DrrA and S23C or C260 in DrrB………….45 Fig. 1.3 Disulfide cross-linking between T311C, S302C, S287C, S253C, or S232C in DrrA and S23C in DrrB… ............................. ...
A Survey of Left-handed Helices in Protein Structures
... very rare. Stretches of amino acids with unusual backbone conformations (e.g. left-handed helices) often appear at ligand-binding sites, protein–protein interfaces or other functional sites. It has been suggested that proteins may sacrifice a part of their stability to form an effective functional s ...
... very rare. Stretches of amino acids with unusual backbone conformations (e.g. left-handed helices) often appear at ligand-binding sites, protein–protein interfaces or other functional sites. It has been suggested that proteins may sacrifice a part of their stability to form an effective functional s ...
Protein Quality Control as a Strategy for Cellular Regulation
... conditions that demand its levels to be altered. Examples include the regulated degradation of p53,4 temporally programmed destruction of cyclins and other cell cycle regulators,5 and the selective degradation of glucose-synthesizing enzymes after feeding.6 In all cases, unique features of the targe ...
... conditions that demand its levels to be altered. Examples include the regulated degradation of p53,4 temporally programmed destruction of cyclins and other cell cycle regulators,5 and the selective degradation of glucose-synthesizing enzymes after feeding.6 In all cases, unique features of the targe ...
universidad complutense de madrid - E-Prints Complutense
... organization, maintenance of nuclear morphology, mechanotransduction, physical connection between the cytoskeleton and the nucleoskeleton etc. These functions are also fulfilled in the plant cells although they lack genes encoding lamins and most lamin-binding proteins. The plant lamina was describe ...
... organization, maintenance of nuclear morphology, mechanotransduction, physical connection between the cytoskeleton and the nucleoskeleton etc. These functions are also fulfilled in the plant cells although they lack genes encoding lamins and most lamin-binding proteins. The plant lamina was describe ...
The proposed channel-enzyme transient receptor potential
... eLife digest Ion channels are proteins that allow specific charged particles to move across the membranes of cells – for example to travel in or out of a cell, or between different parts of the same cell. Almost all ion channels are gated, meaning that they can open and close in response to differen ...
... eLife digest Ion channels are proteins that allow specific charged particles to move across the membranes of cells – for example to travel in or out of a cell, or between different parts of the same cell. Almost all ion channels are gated, meaning that they can open and close in response to differen ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.