Quantitative iTRAQ Proteomics Revealed Possible Roles for
... among which 4375–5082 proteins were found to be present at sufficient amounts to be reliably quantified (Table 1). To evaluate the quantitative precision and reproducibility of these analyses, a linear regression analysis was carried out. As an example, Figure 3 depicts the plots of the replicates o ...
... among which 4375–5082 proteins were found to be present at sufficient amounts to be reliably quantified (Table 1). To evaluate the quantitative precision and reproducibility of these analyses, a linear regression analysis was carried out. As an example, Figure 3 depicts the plots of the replicates o ...
Effects of 6-Thioguanine on RNA Biosynthesis in Regenerating Rat
... cleotide not inhibit the reaction, but in contrast, it was found to partially substitute for the natural substrate. Gray and Rachmeler (6) reported that 6-TG was incorporated into Escherichia coli tRNA and that this incorporation appar ently affected the amino acid acceptor activities of some of the ...
... cleotide not inhibit the reaction, but in contrast, it was found to partially substitute for the natural substrate. Gray and Rachmeler (6) reported that 6-TG was incorporated into Escherichia coli tRNA and that this incorporation appar ently affected the amino acid acceptor activities of some of the ...
SURVEY AND SUMMARY Origins of tmRNA: the
... associated with Small protein B (SmpB). tmRNA is a hybrid molecule carrying out both transfer and messenger RNA activities, and its total length varies between about 260 and 430 nucleotides, depending on the cell species (Figure 4). It is always aminoacylated by alanine. As for SmpB, its topology ma ...
... associated with Small protein B (SmpB). tmRNA is a hybrid molecule carrying out both transfer and messenger RNA activities, and its total length varies between about 260 and 430 nucleotides, depending on the cell species (Figure 4). It is always aminoacylated by alanine. As for SmpB, its topology ma ...
RNA Structure
... mRNA. One is rRNA. XXVII. Ribosomal RNA (rRNA) [S27] a. rRNA is composed of two parts. One part is RNA the second part is protein. 2/3 of ribosome is RNA and 1/3 is protein. So it is a RNA-protein complex. b. Ribosome is one to translate mRNA into protein. The role of rRNA is to serve as a scaffold ...
... mRNA. One is rRNA. XXVII. Ribosomal RNA (rRNA) [S27] a. rRNA is composed of two parts. One part is RNA the second part is protein. 2/3 of ribosome is RNA and 1/3 is protein. So it is a RNA-protein complex. b. Ribosome is one to translate mRNA into protein. The role of rRNA is to serve as a scaffold ...
involvement of protein tyrosine phosphorylation of human sperm in
... the most prevalent form of post-translational modification in metazoan cell and along with allosteric modulation, is recognized as a universal mechanism for regulating function of proteins involved in many biological processes. Receptors for several growth factors are themselves tyrosine protein kin ...
... the most prevalent form of post-translational modification in metazoan cell and along with allosteric modulation, is recognized as a universal mechanism for regulating function of proteins involved in many biological processes. Receptors for several growth factors are themselves tyrosine protein kin ...
X-ray structure of the C-terminal domain of a coronavirus nucle
... was further purified by heparin affinity chromatography, concentrated to 1-2 mg/ml and was checked for monodispersity by dynamic light scattering (Dynapro) and negative stain electron microscopy. Limited proteolytic cleavage of full length N protein (1-2 mg/ml) was carried out with 2% (wt trypsin /w ...
... was further purified by heparin affinity chromatography, concentrated to 1-2 mg/ml and was checked for monodispersity by dynamic light scattering (Dynapro) and negative stain electron microscopy. Limited proteolytic cleavage of full length N protein (1-2 mg/ml) was carried out with 2% (wt trypsin /w ...
Comparative proteomic network signatures in seminal plasma of
... DEPs were obtained by applying different constraints for significance tests and/or fold-change cutoffs based on the average SpC of the protein from multiple runs. Appropriate filters were used to identify DEPs that were ...
... DEPs were obtained by applying different constraints for significance tests and/or fold-change cutoffs based on the average SpC of the protein from multiple runs. Appropriate filters were used to identify DEPs that were ...
Process Biochemistry
... Another important result is the insignificant differences among diverse ways of storing the raw protein materials, which contradicts the common belief that freezing has negative effects on the solubility of the proteins from raw materials collected for industrial processing [24]. Our results agree wi ...
... Another important result is the insignificant differences among diverse ways of storing the raw protein materials, which contradicts the common belief that freezing has negative effects on the solubility of the proteins from raw materials collected for industrial processing [24]. Our results agree wi ...
Methods and approaches for the comprehensive characterization
... of peptides. These complex mixtures need to be fractionated before further analysis. Initial proteomic separation techniques were based on gel electrophoresis, either one-dimensional (1-DE) or two-dimensional electrophoresis (2-DE), in which proteins or peptides are separated based on their charge a ...
... of peptides. These complex mixtures need to be fractionated before further analysis. Initial proteomic separation techniques were based on gel electrophoresis, either one-dimensional (1-DE) or two-dimensional electrophoresis (2-DE), in which proteins or peptides are separated based on their charge a ...
Chapter 8
... Enhancers: regulatory sequences located farther away from the start site. First identified in studies of the promoter of virus SV40. Activity of enhancers does not depend on their distance from, or orientation with respect to the transcription ...
... Enhancers: regulatory sequences located farther away from the start site. First identified in studies of the promoter of virus SV40. Activity of enhancers does not depend on their distance from, or orientation with respect to the transcription ...
Studies of focal adhesion assembly
... specific contacts with the talin PTB domain. Structurebased mutagenesis also showed that these specific membrane proximal contacts with the β tail are required for integrin activation and that they are unique to the talin PTB domain ...
... specific contacts with the talin PTB domain. Structurebased mutagenesis also showed that these specific membrane proximal contacts with the β tail are required for integrin activation and that they are unique to the talin PTB domain ...
Abiogenesis
... • RNA strings merge with bilayer shells – Lipid bilayers form ‘vesicles’ or shells ...
... • RNA strings merge with bilayer shells – Lipid bilayers form ‘vesicles’ or shells ...
Structure-activity Relationships in Flexible Protein Domains
... be established. Overall, when exogenously introduced into cells the GDIs behave as negative regulators by maintaining rho GTPases as inactive cytosolic forms which are unable to effectively interact with GEFs and/or downstream target molecules. However, in some situations, for example, ezrin/radixin ...
... be established. Overall, when exogenously introduced into cells the GDIs behave as negative regulators by maintaining rho GTPases as inactive cytosolic forms which are unable to effectively interact with GEFs and/or downstream target molecules. However, in some situations, for example, ezrin/radixin ...
video slide - Biology at Mott
... Some genes can encode more than one kind of polypeptide, depending on which segments are treated as exons during RNA splicing Such variations are called alternative RNA splicing Because of alternative splicing, the number of different proteins an organism can produce is much greater than its number ...
... Some genes can encode more than one kind of polypeptide, depending on which segments are treated as exons during RNA splicing Such variations are called alternative RNA splicing Because of alternative splicing, the number of different proteins an organism can produce is much greater than its number ...
Iron-sulfur proteins and the role of iron in the gene expression of
... units can be further combined in clusters that contain up to eight iron ions. Simple clusters are coordinated in proteins through iron bound by cysteinyl ligands or, less often, by the mixture of cysteinyl and histidyl ligands. In complex clusters, which can be derived from simple precursors by chem ...
... units can be further combined in clusters that contain up to eight iron ions. Simple clusters are coordinated in proteins through iron bound by cysteinyl ligands or, less often, by the mixture of cysteinyl and histidyl ligands. In complex clusters, which can be derived from simple precursors by chem ...
Manual: Universal Human miRNA Reference RNA
... Stratagene Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis ...
... Stratagene Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis ...
Document
... • Introns are spliced out of pre-mRNAs to produce the mature mRNA that is translated. • Alternative splicing recognizes different splice sites in different tissue types. • The mature mRNAs in each tissue possess different exons, resulting in different polypeptide products from the same gene. ...
... • Introns are spliced out of pre-mRNAs to produce the mature mRNA that is translated. • Alternative splicing recognizes different splice sites in different tissue types. • The mature mRNAs in each tissue possess different exons, resulting in different polypeptide products from the same gene. ...
SpoIIQ Anchors Membrane Proteins on Both Sides of
... A powerful system in which to identify the anchors responsible for localizing proteins at particular sites is the process of sporulation in Bacillus subtilis. Many proteins involved in spore formation localize to defined subcellular positions and this localization is critical for their activity. Upo ...
... A powerful system in which to identify the anchors responsible for localizing proteins at particular sites is the process of sporulation in Bacillus subtilis. Many proteins involved in spore formation localize to defined subcellular positions and this localization is critical for their activity. Upo ...
Myosin binding proteins - Journal of Cell Science
... understanding of this protein family we have characterized the domains in each of these two proteins which are required for targeting the proteins to their native site(s) in the sarcomere during myogenesis. Cultures of skeletal muscle myoblasts were transfected with expression plasmids encoding muta ...
... understanding of this protein family we have characterized the domains in each of these two proteins which are required for targeting the proteins to their native site(s) in the sarcomere during myogenesis. Cultures of skeletal muscle myoblasts were transfected with expression plasmids encoding muta ...
local copy pdf
... for helping to reveal the role of telomeres, the end bits of chromosomes that help protect genetic instructions during cell division. But more than a decade ago, Szostak shifted his lab’s focus to exploring how life on Earth may have gotten its start. He would dearly love to know the recipe for the ...
... for helping to reveal the role of telomeres, the end bits of chromosomes that help protect genetic instructions during cell division. But more than a decade ago, Szostak shifted his lab’s focus to exploring how life on Earth may have gotten its start. He would dearly love to know the recipe for the ...
Initial characterization of ayrRABC
... he proper localization of many proteins requires their translocation across one or more membranes. The general secretory (Sec) pathway, conserved throughout bacteria, is the canonical translocation pathway and is responsible for translocating the vast majority of secreted proteins across the cytopla ...
... he proper localization of many proteins requires their translocation across one or more membranes. The general secretory (Sec) pathway, conserved throughout bacteria, is the canonical translocation pathway and is responsible for translocating the vast majority of secreted proteins across the cytopla ...
What are Membranes?
... FIGURE 11-19 Restricted motion of the erythrocyte chloride-bicarbonate exchanger and glycophorin. The proteins span the membrane and are tethered to spectrin, a cytoskeletal protein, by another protein, ankyrin, limiting their lateral mobility. Ankyrin is anchored in the membrane by a covalently bo ...
... FIGURE 11-19 Restricted motion of the erythrocyte chloride-bicarbonate exchanger and glycophorin. The proteins span the membrane and are tethered to spectrin, a cytoskeletal protein, by another protein, ankyrin, limiting their lateral mobility. Ankyrin is anchored in the membrane by a covalently bo ...
GPCR–G fusion proteins
... and G proteins19–22.) The efficiency of GPCR–G-protein coupling depends on the ratio of GPCRs to G proteins and on the absolute concentrations of each23. In addition, segregation of receptors and G proteins into different membrane microdomains constrains signal output24. The precise determination of ...
... and G proteins19–22.) The efficiency of GPCR–G-protein coupling depends on the ratio of GPCRs to G proteins and on the absolute concentrations of each23. In addition, segregation of receptors and G proteins into different membrane microdomains constrains signal output24. The precise determination of ...
Structure and function of the chloroplast signal recognition particle
... therefore posed the question which structural characteristics of the LHCPs determine the transit complex formation with cpSRP. By analogy, with the co-translational targeting system where binding of cytosolic SRP54 to a substrate protein is mediated via its hydrophobic signal sequence, it was shown ...
... therefore posed the question which structural characteristics of the LHCPs determine the transit complex formation with cpSRP. By analogy, with the co-translational targeting system where binding of cytosolic SRP54 to a substrate protein is mediated via its hydrophobic signal sequence, it was shown ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.