Comparison of sequence-based and structure
... the families have low average sequence identities, three families have high average %I but have poor correlation coefficient. These families are either from multi-domain or small protein classes. Further investigations on these three families revealed possible reasons for the poor correlation between ...
... the families have low average sequence identities, three families have high average %I but have poor correlation coefficient. These families are either from multi-domain or small protein classes. Further investigations on these three families revealed possible reasons for the poor correlation between ...
Urine Reflection of Changes in Blood
... In paper published on Science China Life Science [5], two different anticoagulants were used (unfractionated heparin and argatroban) to disrupt the blood coagulation status of adult female SD rats. Changes in urine and plasma proteomic profiles in response to treatment with the drugs were compared wi ...
... In paper published on Science China Life Science [5], two different anticoagulants were used (unfractionated heparin and argatroban) to disrupt the blood coagulation status of adult female SD rats. Changes in urine and plasma proteomic profiles in response to treatment with the drugs were compared wi ...
Imaging Single-mRNA Localization and Translation in Live Neurons
... (Dictenberg et al., 2008) and for mRNA delivery to dendritic spines in response to synaptic stimulation (Kao et al., 2010). Live imaging of neurons also showed that Huntingtin (Htt), huntingtin-associated protein 1 (HAP1), KIF5A, and dynein intermediate chain are associated with -actin mRNA during ...
... (Dictenberg et al., 2008) and for mRNA delivery to dendritic spines in response to synaptic stimulation (Kao et al., 2010). Live imaging of neurons also showed that Huntingtin (Htt), huntingtin-associated protein 1 (HAP1), KIF5A, and dynein intermediate chain are associated with -actin mRNA during ...
The Phosphoproteome of a Chlamydomonas reinhardtii Eyespot
... and Sickmann, 2005). This is due to a few facts. (1) Phosphoproteins can have more than one phosphorylation site and the phosphorylation status of these sites can fluctuate, depending on the physiological conditions of the cells. (2) Only a small portion of a given protein in the cell can be phospho ...
... and Sickmann, 2005). This is due to a few facts. (1) Phosphoproteins can have more than one phosphorylation site and the phosphorylation status of these sites can fluctuate, depending on the physiological conditions of the cells. (2) Only a small portion of a given protein in the cell can be phospho ...
Protein Posttranslational Modifications: The Chemistry of Proteome
... in eukaryotic genomes to generate diversity in the corresponding proteomes, the inventory of all proteins in a cell or organism. Proteomes may be two to three orders of magnitude more complex (> 1 000 000 molecular species of proteins) than the encoding genomes would predict. The first route of dive ...
... in eukaryotic genomes to generate diversity in the corresponding proteomes, the inventory of all proteins in a cell or organism. Proteomes may be two to three orders of magnitude more complex (> 1 000 000 molecular species of proteins) than the encoding genomes would predict. The first route of dive ...
Molecular mechanisms of Salmonella invasion
... regions that likely span the inner membrane [38], and some homologues of these proteins, identified in other pathogens, are known to be membrane proteins. Thus, Spa proteins are probably structural components which assist in the translocation process, since non-polar mutations in the spa genes aboli ...
... regions that likely span the inner membrane [38], and some homologues of these proteins, identified in other pathogens, are known to be membrane proteins. Thus, Spa proteins are probably structural components which assist in the translocation process, since non-polar mutations in the spa genes aboli ...
P4-0065 RNA/DNA/Protein Purification Kit
... • Optional: The use of β-mercaptoethanol in lysis is highly recommended for most tissues, particularly those known to have high RNAse content (ex: pancreas). It is also recommended for users who wish to isolate RNA for sensitive downstream applications. Add 10 µL of β-mercaptoethanol (provided by th ...
... • Optional: The use of β-mercaptoethanol in lysis is highly recommended for most tissues, particularly those known to have high RNAse content (ex: pancreas). It is also recommended for users who wish to isolate RNA for sensitive downstream applications. Add 10 µL of β-mercaptoethanol (provided by th ...
Snapshots of RNA polymerase II transcription initiation
... either TBP crosslinking to DNA is indirect via one of these other transcription factors or, more likely, that TBP is not permitted to dwell on inactive promoters in vivo. The second hypothesis is supported by the observation that mutation of Mot1, a protein that can dissociate DNAbound TBP [29,30], ...
... either TBP crosslinking to DNA is indirect via one of these other transcription factors or, more likely, that TBP is not permitted to dwell on inactive promoters in vivo. The second hypothesis is supported by the observation that mutation of Mot1, a protein that can dissociate DNAbound TBP [29,30], ...
9.3 The Three-Dimensional Structure of Proteins, Continued
... 9.3 The Three-Dimensional Structure of Proteins, Continued • The helix is a coiled structure, and much like the coil of a telephone cord, it is a right-handed coil. • This coil is stabilized by hydrogen bonds between the carbonyl oxygen of one amino acid and the N—H hydrogen atom of another amino ...
... 9.3 The Three-Dimensional Structure of Proteins, Continued • The helix is a coiled structure, and much like the coil of a telephone cord, it is a right-handed coil. • This coil is stabilized by hydrogen bonds between the carbonyl oxygen of one amino acid and the N—H hydrogen atom of another amino ...
Ubiquinone
... containing paracrystalline structures. • This lysyl-tRNA mutation is also one of the causes of adult-onset (type II) diabetes. ...
... containing paracrystalline structures. • This lysyl-tRNA mutation is also one of the causes of adult-onset (type II) diabetes. ...
Overview for Electrophoresis and Western Blotting
... The major advantages of discontinuous buffer systems are that relatively large volumes of dilute protein samples can be applied to the gel and resolution is much greater than that obtained with continuous systems. This increased resolution is a direct result of the way proteins concentrate, or stack ...
... The major advantages of discontinuous buffer systems are that relatively large volumes of dilute protein samples can be applied to the gel and resolution is much greater than that obtained with continuous systems. This increased resolution is a direct result of the way proteins concentrate, or stack ...
Intro to Cell Biology - Mrs. Florio's Science Class
... ACIDS used by cells ____________ to ________________ make proteins ...
... ACIDS used by cells ____________ to ________________ make proteins ...
“molecular” versus “colloidal”: controversies in biology and
... strongly opposed to another concept of sharp specificity, the notion of distinguishable bacterial species, put forward by Ferdinand Cohn and, later, Robert Koch (21). Colloidal chemists, whose explanations relied on physical concepts such as adsorption and electrical properties, which allowed only f ...
... strongly opposed to another concept of sharp specificity, the notion of distinguishable bacterial species, put forward by Ferdinand Cohn and, later, Robert Koch (21). Colloidal chemists, whose explanations relied on physical concepts such as adsorption and electrical properties, which allowed only f ...
Dirty Business - American Chemical Society
... helps in the metabolism of sugars and organic acids Phenylalanine - Influences certain chemicals in the brain that relate to pain sensation. Helpful for some people with Parkinson’s disease and has been used to treat chronic pain. It is used in elevating the mood since it is so closely involved with ...
... helps in the metabolism of sugars and organic acids Phenylalanine - Influences certain chemicals in the brain that relate to pain sensation. Helpful for some people with Parkinson’s disease and has been used to treat chronic pain. It is used in elevating the mood since it is so closely involved with ...
Identification and Characterization of Two Temperature
... research on S. suis in recent years, little is known about pathogenesis and virulence factors. Studies on the pathogenicity of S. suis are complicated by the presence of multiple serotypes (of which currently 35 are known) based on the capsular polysaccharides (CPS) and by the high diversity in leve ...
... research on S. suis in recent years, little is known about pathogenesis and virulence factors. Studies on the pathogenicity of S. suis are complicated by the presence of multiple serotypes (of which currently 35 are known) based on the capsular polysaccharides (CPS) and by the high diversity in leve ...
Targeting Infectious Disease: Structural and functional
... pathway, a pathway that produces isoprenoid precursors. Isoprenoids are compounds essential to all living organisms, and the isoprenoid precursors can be produced by two distinct pathways. In humans, isoprenoid precursors are synthesized by the mevalonate pathway. In contrast, mycobacteria and proto ...
... pathway, a pathway that produces isoprenoid precursors. Isoprenoids are compounds essential to all living organisms, and the isoprenoid precursors can be produced by two distinct pathways. In humans, isoprenoid precursors are synthesized by the mevalonate pathway. In contrast, mycobacteria and proto ...
Based Method for Identification of Horizontal Gene Transfer in Bacteria
... These are molecules that, when joined together, make up the structural units of RNA and DNA. A nucleotide is composed of a nucleobase (nitrogenous base), a five-carbon sugar (either ribose or 2'-deoxyribose), and one to three phosphate groups. The International Union of Pure and Applied Chemistry (I ...
... These are molecules that, when joined together, make up the structural units of RNA and DNA. A nucleotide is composed of a nucleobase (nitrogenous base), a five-carbon sugar (either ribose or 2'-deoxyribose), and one to three phosphate groups. The International Union of Pure and Applied Chemistry (I ...
Introduction
... • try to find some function which often works • do not stick to real physics Will my drug dissolve in water or oil (lipid) ? (important) • sounds like chemistry • usually approached by machine learning • number of atoms, types of atoms, … http://www.cgl.ucsf.edu/chimera/ImageGallery/, bluetongue vir ...
... • try to find some function which often works • do not stick to real physics Will my drug dissolve in water or oil (lipid) ? (important) • sounds like chemistry • usually approached by machine learning • number of atoms, types of atoms, … http://www.cgl.ucsf.edu/chimera/ImageGallery/, bluetongue vir ...
Targeting to the T. gondii plastid
... is mediated by an N-terminal bipartite targeting sequence composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis ( ...
... is mediated by an N-terminal bipartite targeting sequence composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis ( ...
Document
... DNA modifications, which lead in the regulation of specific genes. ds RNA dependent mechanism can act at both transcriptional as well as post transcriptional levels. This type of gene expression is given different names in ...
... DNA modifications, which lead in the regulation of specific genes. ds RNA dependent mechanism can act at both transcriptional as well as post transcriptional levels. This type of gene expression is given different names in ...
Mitochondrial quality control by the ubiquitin
... Vms1 regulates mitochondrial protein degradation We recently identified a mechanism in yeast whereby a novel protein, which we have named Vms1 [VCP (valosincontaining protein) (p97)/Cdc48-associated mitochondrialstress-responsive 1], recruits Cdc48/p97 and Npl4 to stressed mitochondria [15]. Under n ...
... Vms1 regulates mitochondrial protein degradation We recently identified a mechanism in yeast whereby a novel protein, which we have named Vms1 [VCP (valosincontaining protein) (p97)/Cdc48-associated mitochondrialstress-responsive 1], recruits Cdc48/p97 and Npl4 to stressed mitochondria [15]. Under n ...
Electrophoresis
... • Analytical uses of this technique center on protein nucleic acid characterization (e.g. purity, size, or molecular weight, and composition) • Acrylamide is neurotoxin, however, the reagents must be ...
... • Analytical uses of this technique center on protein nucleic acid characterization (e.g. purity, size, or molecular weight, and composition) • Acrylamide is neurotoxin, however, the reagents must be ...
Membrane nanodomains in plants: capturing form, function, and
... The plasma membrane is the interface between the cell and the external environment. Plasma membrane lipids provide scaffolds for proteins and protein complexes that are involved in cell to cell communication, signal transduction, immune responses, and transport of small molecules. In animals, fungi, ...
... The plasma membrane is the interface between the cell and the external environment. Plasma membrane lipids provide scaffolds for proteins and protein complexes that are involved in cell to cell communication, signal transduction, immune responses, and transport of small molecules. In animals, fungi, ...
Human Dcp2: a catalytically active mRNA decapping enzyme
... human a-actinin (ABD). To test for decapping activity, these proteins were incubated with an in vitro transcribed RNA 49 nucleotides in length carrying a labelled cap structure. The products of the reaction were fractionated by thin layer chromatography (TLC), revealed by autoradiography and identi® ...
... human a-actinin (ABD). To test for decapping activity, these proteins were incubated with an in vitro transcribed RNA 49 nucleotides in length carrying a labelled cap structure. The products of the reaction were fractionated by thin layer chromatography (TLC), revealed by autoradiography and identi® ...
chemistry bulletin 2005
... W, Ureaplasma urealyticum, Mycoplasma pneumoniae, Mycoplasma genitalium. ...
... W, Ureaplasma urealyticum, Mycoplasma pneumoniae, Mycoplasma genitalium. ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.