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Chap 3
Chap 3

... (2) lagging strand: synthesized discontinuously (Fig. 3-3). Okazaki fragments can act as a primer. The nicks between fragments are sealed by DNA ligase. 9. DNA polymerase I has both 3’-5’and 5’-3’exonuclease activity (remove or excise nucleotides just added). This “proofreading” function ensures fid ...
Document
Document

Chapter 17 - TeacherWeb
Chapter 17 - TeacherWeb

... transfer RNA Small, ~80 nucleotides long. tRNA exists as a single-stranded molecule. However, regions of double helix can form where there is some base pair complementation (U and A , G and C), resulting in hairpin loops. The RNA molecule with its hairpin loops is said to have a secondary structure ...
242140_Fx_DNA-RNA
242140_Fx_DNA-RNA

... 10. What are the names of the gene and the enzyme responsible for the glowing in a firefly’s tail? 11. After finding the correct gene, what does RNA Polymerase actually do? 12. After transcription, what happens to the mRNA strand? (Where in the cell ...
DNA to Protein Synthesis
DNA to Protein Synthesis

... DNA must be copied to messenger RNA (mRNA) mRNA goes from nucleus to the ribosomes in cytoplasm mRNA complements known as codons ...
Transactivation Assay Introduction Regulation of gene expression at
Transactivation Assay Introduction Regulation of gene expression at

... Regulation of gene expression at the level of transcription is one of the most efficient means for cells to change their function and/or respond to changes in their environment. (How else can cells regulate gene expression?*). Eukaryotic transcription is regulated by a segment of DNA called an enhan ...
Molecular evolution - Integrative Biology
Molecular evolution - Integrative Biology

... “The Gene Ontology project provides an ontology of defined terms representing gene product properties. The ontology covers three domains: cellular component, the parts of a cell or its extracellular environment; molecular function, the elemental activities of a gene product at the molecular level, s ...
Recombinant DNA and gene cloning To use an unique feature(s) of
Recombinant DNA and gene cloning To use an unique feature(s) of

... To use an unique feature(s) of your gene/gene product to isolate the DNA fragment containing your gene from a library of DNA fragments. Difficulty in isolating genes (needle in a long line of connected needles): A gene is a small part of a large DNA (0.01% of an average chromosome); DNA pieces all h ...
Prodigiosin Production in E. Coli
Prodigiosin Production in E. Coli

... of Gram negative, rod shaped bacteria ...
Regulation of gene e
Regulation of gene e

... After translation, various types of protein processing, including cleavage and the addition of chemical groups, are subject to control Proteasomes are giant protein complexes that bind protein molecules and degrade ...
DNA, RNA and Protein Synthesis 1. Define: Nucleotide
DNA, RNA and Protein Synthesis 1. Define: Nucleotide

... Nucleotide – Nucleotides are small, organic molecules made up of a pentose sugar (ribose or deoxyribose), a phosphate group and one nitrogenous base (adenine, guanine, cytosine, thymine or uracil). Nucleotides are used as the "building blocks" of nucleic acids (DNA and RNA). They are also used to fo ...
The Biocreative Task in SEER
The Biocreative Task in SEER

... Gene Entity Pitfalls • Language is complex Stably transfected human kidney 293 cells expressing the wild type rat LH / CG receptor ( rLHR ) or receptors with C-terminal tails truncated at residues 653 , 631 , or 628 (designated rLHR-t653 , rLHR-t631 , and rLHR-t628 ) were used to probe the importan ...
Epigenetic regulators as novel treatments
Epigenetic regulators as novel treatments

... Some definitions: Epigenetics-the study of heritable changes in gene expression without changing the DNA sequence; this occurs at 3 levels of organization: 1) methylation of cytosine nucleotides within coding sequences and at promoter sites that alter transcription rates 2) changes in chromatin pro ...
Exam V2002 - English
Exam V2002 - English

... Immunoglobulins consist of heavy and light chains which are both composed of variable and constant amino acid sequences (Figure 14.7). In early B-lymphocyte (or T-cell) development the genes for the immunoglobulin proteins are assembled by recombination from gene segments that code for the variable ...
university of oslo
university of oslo

... Immunoglobulins consist of heavy and light chains which are both composed of variable and constant amino acid sequences (Figure 14.7). In early B-lymphocyte (or T-cell) development the genes for the immunoglobulin proteins are assembled by recombination from gene segments that code for the variable ...
5b Gene Expression
5b Gene Expression

... - The Nature of Chromosomes - The Cell Cycle - Mitosis and Cytokinesis - Cancer Cells: Abnormal Cell Cycle • The Expression of Genes as Proteins: DNA gene --> RNA --> Protein - Transcription by RNA Polymerase (DNA gene --> mRNA) - The Three Types of RNA ...
Does your DNA define you Ans
Does your DNA define you Ans

... Now we have more understanding of the epigenome and how it is related to health and disease, this knowledge can be exploited to help develop drugs which change gene expression profiles. Unlike the genome which is largely static, the epigenome is more dynamic and we have more influence over it. Since ...
Analytical and Chromatography - Sigma
Analytical and Chromatography - Sigma

A Probabilistic Model to Integrate Microarray and ChIP Data
A Probabilistic Model to Integrate Microarray and ChIP Data

... • ChIP data is notoriously noisy; for example the same transcription factor (MSN4) in the same conditions (rich medium) is found to bind 32 genes in Lee et al. and 57 genes in Harbison et al. (the intersection is 20 genes). • Posterior estimation helps with false positives, not with ...
What is RNA, and How Does it Differ from DNA?
What is RNA, and How Does it Differ from DNA?

Biology 212 General Genetics
Biology 212 General Genetics

A graph-theoretic modeling on GO space for biological interpretation
A graph-theoretic modeling on GO space for biological interpretation

... Not only DNA microarray data, but also any kinds of group analysis with any ontology having an identical structure with GO ...
Chapter 17: Gene Expression Gene Expression DNA houses all
Chapter 17: Gene Expression Gene Expression DNA houses all

...  The Genetic Code o Must code for 20 amino acids o Codon  If each base a ‘letter’, how long word?  1 letter = 4 words  2 letters = 16 words  3 letters = 64 words  61 codons for AAs, 3 for ‘stop’ o Uniform Across Species ...
A1983QZ35500002
A1983QZ35500002

... amphibians, fish, and certain insects are known to amplify their ribosomal RNA genes, and there are now examples of amplification of genes for proteins. A phenomenon that I termed ‘forced gene amplification’ is a response by which cells become resistant to a drug by amplifying the gene whose product ...
Apoptosis-associtated pathways are induced vy Phytophthora
Apoptosis-associtated pathways are induced vy Phytophthora

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