Test 3
... Regulatory gene Gal4 binds to UASG as a dimer in absence of Galatose Gal80P binds to Gal4 and prevents it from acting as an activator In presence of Galatose Galactose binds to Gal3P Gal3p binds to GAL80P Binding of GAL3p to GAL80p allows Gal4 to act as activator Now HMG protein allow DNA to bend SW ...
... Regulatory gene Gal4 binds to UASG as a dimer in absence of Galatose Gal80P binds to Gal4 and prevents it from acting as an activator In presence of Galatose Galactose binds to Gal3P Gal3p binds to GAL80P Binding of GAL3p to GAL80p allows Gal4 to act as activator Now HMG protein allow DNA to bend SW ...
25 transcription, translation
... What are the three stages of DNA to protein? Where does each occur? Walk through each. There were 4 different RNAs involved: Identify each along with its use. ...
... What are the three stages of DNA to protein? Where does each occur? Walk through each. There were 4 different RNAs involved: Identify each along with its use. ...
Slide 1
... from pyruvate carboxylase using UCSC table browser and Notepad++ 1.5 Construct in both cases sequence logo and frequency plot. Can you identify (regulatory) sequence motifs? ...
... from pyruvate carboxylase using UCSC table browser and Notepad++ 1.5 Construct in both cases sequence logo and frequency plot. Can you identify (regulatory) sequence motifs? ...
Finding genes and detecting mutations
... insertions/deletions of < 10bp are harder to detect. Small changes such as single base mutations can be detected in many ways • Purify DNA fragment to be analysed, usually by PCR. A label (radioactive or fluorescent) can be incorporated at this stage. – You can also start with mRNA, by first reverse ...
... insertions/deletions of < 10bp are harder to detect. Small changes such as single base mutations can be detected in many ways • Purify DNA fragment to be analysed, usually by PCR. A label (radioactive or fluorescent) can be incorporated at this stage. – You can also start with mRNA, by first reverse ...
Positive Gene Regulation
... Regulatory proteins stimulate gene expression by binding DNA & stimulating transcription (positive control) or binding to repressors to inactivate repressor function. Some genes are continuously expressed; they are always turned “on” EX: ribosomal genes ...
... Regulatory proteins stimulate gene expression by binding DNA & stimulating transcription (positive control) or binding to repressors to inactivate repressor function. Some genes are continuously expressed; they are always turned “on” EX: ribosomal genes ...
Gene Expression
... • tRNA is a single strand of RNA that is folded into the shape of a clover. It has an anticodon that matches the codon on the mRNA, and a spot for holding the amino acid that matches the codon. ...
... • tRNA is a single strand of RNA that is folded into the shape of a clover. It has an anticodon that matches the codon on the mRNA, and a spot for holding the amino acid that matches the codon. ...
BiGCaT
... Figure 9-87. Control of the poly-A tail length affects both mRNA stability and mRNA translation. (A) Most translated mRNAs have poly-A tails that exceed a minimum length of about 30 As. The tails on selected mRNAs can be either elongated or rapidly cleaved in the cytosol, and this will have an effe ...
... Figure 9-87. Control of the poly-A tail length affects both mRNA stability and mRNA translation. (A) Most translated mRNAs have poly-A tails that exceed a minimum length of about 30 As. The tails on selected mRNAs can be either elongated or rapidly cleaved in the cytosol, and this will have an effe ...
Unit Three “Cell Proliferation and Genetics”
... Categories of RNA • Ribosomal RNA (rRNA) – DNA serves as template for production of rRNA; formed in the nucleus of a cell; moves into the cytoplasm to bond with proteins; rRNA + proteins make up Ribosomes (site of protein synthesis) • Messenger RNA (mRNA) – DNA serves as template for production of ...
... Categories of RNA • Ribosomal RNA (rRNA) – DNA serves as template for production of rRNA; formed in the nucleus of a cell; moves into the cytoplasm to bond with proteins; rRNA + proteins make up Ribosomes (site of protein synthesis) • Messenger RNA (mRNA) – DNA serves as template for production of ...
DNA, RNA, and Proteins part 2 - Tri-City
... and enzymes that regulate body functions, are all made of proteins. Name some parts of the human body that contain ...
... and enzymes that regulate body functions, are all made of proteins. Name some parts of the human body that contain ...
Human Genetics
... AAs to ribosomes. The AA’s join in cytoplasm to form proteins. 20 types. Loop structure – Ribosomal RNA: (rRNA) Joins with proteins made in cytoplasm to form the subunits of ribosomes. Linear ...
... AAs to ribosomes. The AA’s join in cytoplasm to form proteins. 20 types. Loop structure – Ribosomal RNA: (rRNA) Joins with proteins made in cytoplasm to form the subunits of ribosomes. Linear ...
Diversity of genomes and the tree of life
... related genes that have resulted from a gene duplication event within a single genome, and are likely to have diverged in their function ...
... related genes that have resulted from a gene duplication event within a single genome, and are likely to have diverged in their function ...
Biology Genetics Unit: Online Activities 1.) Go to the link: http://learn
... 1.) Go to the link: http://learn.genetics.utah.edu/content/begin/dna/ Run through the “What is DNA?” interactive. This will act as a review. A.) What type of cells are examined? _________________________________ B.) What molecule contains all the genetic material necessary for a cell to carry on all ...
... 1.) Go to the link: http://learn.genetics.utah.edu/content/begin/dna/ Run through the “What is DNA?” interactive. This will act as a review. A.) What type of cells are examined? _________________________________ B.) What molecule contains all the genetic material necessary for a cell to carry on all ...
Chapter 10 Topic: RNA transcription Main concepts: •Beadle and
... • “Transcribe and Translate a Gene” http://learn.genetics.utah.edu/units/basics/transcribe/ is an interactive animation that walks you through transcription and translation. Notice that you must first find the “start” codon before the reading frame will appear. • “DNA transcription tutorial” http:// ...
... • “Transcribe and Translate a Gene” http://learn.genetics.utah.edu/units/basics/transcribe/ is an interactive animation that walks you through transcription and translation. Notice that you must first find the “start” codon before the reading frame will appear. • “DNA transcription tutorial” http:// ...
CHAPTER 18 LECTURE NOTES: CONTROL OF GENE
... In Drosophila, differential splicing of one mRNA transcript (sxl) initiates a cascade that eventually determines the sex characteristics of the fly. A transcription factor that activates a promoter of the sxl gene early in development is encoded on the X chromosome of flies. This factor functions as ...
... In Drosophila, differential splicing of one mRNA transcript (sxl) initiates a cascade that eventually determines the sex characteristics of the fly. A transcription factor that activates a promoter of the sxl gene early in development is encoded on the X chromosome of flies. This factor functions as ...
Protein Synthesis
... polymerase binds to these promoters • DNA molecule in the region separates when RNA polymerase binds • Template: separated DNA chain used for transcription • RNA polymerase adds complementary RNA nucleotides to the newly forming RNA molecule (uses complementary base pairing) ...
... polymerase binds to these promoters • DNA molecule in the region separates when RNA polymerase binds • Template: separated DNA chain used for transcription • RNA polymerase adds complementary RNA nucleotides to the newly forming RNA molecule (uses complementary base pairing) ...
Nucleic Acids and Protein Synthesis
... Transcription (continued) • RNA polymerase attaches to the first nucleotide and adds on complementary bases using uracil instead of thymine • Continues until the polymerase reaches the termination signal • What do you think a termination signal does? • Termination signal causes the polymerase to r ...
... Transcription (continued) • RNA polymerase attaches to the first nucleotide and adds on complementary bases using uracil instead of thymine • Continues until the polymerase reaches the termination signal • What do you think a termination signal does? • Termination signal causes the polymerase to r ...
adjusted p-value 3.317x10-25 Position in the ranked list of CD40L
... Supplementary Figure 1: Global gene expression changes of CD40L stimulation are highly comparable in distinct Burkitt Lymphoma cell lines (Ramos and BL2). Geneset Enrichment Analyses were utilized to investigate the similarities of the CD40L effects on gene expression profiles of Ramos and BL2 cells ...
... Supplementary Figure 1: Global gene expression changes of CD40L stimulation are highly comparable in distinct Burkitt Lymphoma cell lines (Ramos and BL2). Geneset Enrichment Analyses were utilized to investigate the similarities of the CD40L effects on gene expression profiles of Ramos and BL2 cells ...
Chapter 13: The Genetic Code and Transcription
... proceeds. Chain elongation will proceed under the direction of an enzyme. Transcription will proceed down the entire gene until it encounters a specific nucleotide sequence that acts as a termination sequence. Termination sequences are especially important to prokaryotes because of the proximity of ...
... proceeds. Chain elongation will proceed under the direction of an enzyme. Transcription will proceed down the entire gene until it encounters a specific nucleotide sequence that acts as a termination sequence. Termination sequences are especially important to prokaryotes because of the proximity of ...