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... Currently, it is still in most cases impossible to purify and describe all of the major and minor allergens of natural allergen sources that are active in aqueous buffers. Thanks to the advance in the field of molecular genetics, this problem can be overcome by using cDNAs for recombinant production ...
... Currently, it is still in most cases impossible to purify and describe all of the major and minor allergens of natural allergen sources that are active in aqueous buffers. Thanks to the advance in the field of molecular genetics, this problem can be overcome by using cDNAs for recombinant production ...
INPS: predicting the impact of non-synonymous variations on protein
... about mutations in the same protein site (Wainreb et al., 2011) and ensemble predictors (Pires et al., 2014b). The available methods were trained under different conditions and on different datasets. They address three different questions. Briefly, they can: (i) predict the DDG real values (in regre ...
... about mutations in the same protein site (Wainreb et al., 2011) and ensemble predictors (Pires et al., 2014b). The available methods were trained under different conditions and on different datasets. They address three different questions. Briefly, they can: (i) predict the DDG real values (in regre ...
science - Madison Central High
... toddler, genetics has captured my curiosity1. I’ve always been amazed that the diversity of Earth’s 8.7 million species results from such tiny differences in DNA from one to the next. I hope this Power Guide can help you learn more about why those differences make the difference that they do. Throug ...
... toddler, genetics has captured my curiosity1. I’ve always been amazed that the diversity of Earth’s 8.7 million species results from such tiny differences in DNA from one to the next. I hope this Power Guide can help you learn more about why those differences make the difference that they do. Throug ...
Journal of Bacteriology
... uberolysin (28), butyrivibriocin (29), gassericin (30), and lactocyclicin (31). For this reason, it was assumed that the product of the gene cluster around RBAM_029230 (in the following, named acn) might be involved in the synthesis and processing of a circular peptide which is the putative antibact ...
... uberolysin (28), butyrivibriocin (29), gassericin (30), and lactocyclicin (31). For this reason, it was assumed that the product of the gene cluster around RBAM_029230 (in the following, named acn) might be involved in the synthesis and processing of a circular peptide which is the putative antibact ...
Coordinated concentration changes of transcript and metabolites in Saccharomyces cerevisiae.
... predictions. Studies aimed at addressing this question have relied on computing correlation coefficients between profiles of transcript and metabolite concentrations, which can then be ranked [7] or used to co-cluster the metabolomic and transcriptomic data [8]. However, it is possible that other ty ...
... predictions. Studies aimed at addressing this question have relied on computing correlation coefficients between profiles of transcript and metabolite concentrations, which can then be ranked [7] or used to co-cluster the metabolomic and transcriptomic data [8]. However, it is possible that other ty ...
Similarities between putative transport proteins of plant viruses
... sequence of proteins encoded by CMV strain O (Hayakawa et al., 1989) and the very similar strain Y (Nitta et al., 1988) were not separately analysed. Three ORF I product caulimoviral sequences were used: CaMV, Cabb S isolate (Franck et al., 1980), CERV (Hull et aL, 1986) and FMV (Richins et al., 198 ...
... sequence of proteins encoded by CMV strain O (Hayakawa et al., 1989) and the very similar strain Y (Nitta et al., 1988) were not separately analysed. Three ORF I product caulimoviral sequences were used: CaMV, Cabb S isolate (Franck et al., 1980), CERV (Hull et aL, 1986) and FMV (Richins et al., 198 ...
DO ANTIBODIES RECOGNIZE AMINO ACID SIDE CHAINS OF
... Our results show that two internal image mAbs that mimic the rabbit al allotype contain unique V sequences homologous to the original antigen but in reverse orientation . Synthetic peptide inhibition experiments confirmed the ability of a reversed sequence to resemble the nominal antigenic epitope ...
... Our results show that two internal image mAbs that mimic the rabbit al allotype contain unique V sequences homologous to the original antigen but in reverse orientation . Synthetic peptide inhibition experiments confirmed the ability of a reversed sequence to resemble the nominal antigenic epitope ...
Molecular cloning of a cDNA encoding a novel Ca2+
... judged to be full-length, encoding a polypeptide of 323 amino acids. The C-terminal 114 amino acids of the product were 40^51% similar to a secreted nuclease from Staphylococcus aureus [2], Nuc nuclease [3], and ParB nuclease [4], and 82% similar (62% similarity through the overall stretch) to a gen ...
... judged to be full-length, encoding a polypeptide of 323 amino acids. The C-terminal 114 amino acids of the product were 40^51% similar to a secreted nuclease from Staphylococcus aureus [2], Nuc nuclease [3], and ParB nuclease [4], and 82% similar (62% similarity through the overall stretch) to a gen ...
Supplemental Information
... interface was clear, and the aqueous layer was washed once with chloroform. An equal volume of isopropanol was added to the resulting aqueous extract and the mixture was incubated on crushed dry ice 20-30 min prior to centrifugation (20 min, 15,000 x g). The resulting pellet was dissolved in 50 mM ...
... interface was clear, and the aqueous layer was washed once with chloroform. An equal volume of isopropanol was added to the resulting aqueous extract and the mixture was incubated on crushed dry ice 20-30 min prior to centrifugation (20 min, 15,000 x g). The resulting pellet was dissolved in 50 mM ...
insulin history
... recombinant insulin analog, Humalog (two amino acids are changed, which increases the ability of the body to use the recombinant insulin). Since the introduction of Humulin, many more recombinant protein drugs have been developed, including human growth hormone, erythropoietin, and parathyroid hormo ...
... recombinant insulin analog, Humalog (two amino acids are changed, which increases the ability of the body to use the recombinant insulin). Since the introduction of Humulin, many more recombinant protein drugs have been developed, including human growth hormone, erythropoietin, and parathyroid hormo ...
Protein profiling of the dimorphic, pathogenic fungus,Penicillium
... Results: Whole cell proteins from the early stages of mould and yeast development in P. marneffei were resolved by two-dimensional gel electrophoresis. Selected proteins were recovered and sequenced by capillary-liquid chromatography-nanospray tandem mass spectrometry. Putative identifications were ...
... Results: Whole cell proteins from the early stages of mould and yeast development in P. marneffei were resolved by two-dimensional gel electrophoresis. Selected proteins were recovered and sequenced by capillary-liquid chromatography-nanospray tandem mass spectrometry. Putative identifications were ...
Gene encoding the group B streptococcal protein R4, its
... prototypic reference antisera for R4 in double-diffusion (results not shown). The trypsin-extracted R4 showed a precipitin result with anti-R4 antiserum for both controls. As the classic example of pepsin sensitivity for pepsin at pH2, no precipitin result was shown. At pH4, pH6 and pH8, however, pr ...
... prototypic reference antisera for R4 in double-diffusion (results not shown). The trypsin-extracted R4 showed a precipitin result with anti-R4 antiserum for both controls. As the classic example of pepsin sensitivity for pepsin at pH2, no precipitin result was shown. At pH4, pH6 and pH8, however, pr ...
paper - Lirmm
... based on nucleotide pair matches between respective codons and amino acid substitution probabilities. One of the main differences between this scoring scheme and the one we present further in this paper is that our scores target nucleotide symbols explicitly, and are computed by taking into account ...
... based on nucleotide pair matches between respective codons and amino acid substitution probabilities. One of the main differences between this scoring scheme and the one we present further in this paper is that our scores target nucleotide symbols explicitly, and are computed by taking into account ...
Exploring Tomato Gene Functions Based on Coexpression Modules
... One of the major challenges of plant-systems biology is in understanding genotype-phenotype associations. In that context, biological networks can increase our understanding of how biomolecules interact to function in plants (Fukushima et al., 2009; Stitt et al., 2010). Large-scale data from genome- ...
... One of the major challenges of plant-systems biology is in understanding genotype-phenotype associations. In that context, biological networks can increase our understanding of how biomolecules interact to function in plants (Fukushima et al., 2009; Stitt et al., 2010). Large-scale data from genome- ...
The Role of the C-terminal Tail of the Ribosomal Protein S13 in Pr
... d recombination techn nology utilizzed the reco ombination function an nd enzymess of phage λ (17, 18). f constructing chrom mosomally engineered d λ‐red recombineeringg is a highlly effectivee method for Escherichia a coli strainss. This techn nique is far more conveenient than n rest ...
... d recombination techn nology utilizzed the reco ombination function an nd enzymess of phage λ (17, 18). f constructing chrom mosomally engineered d λ‐red recombineeringg is a highlly effectivee method for Escherichia a coli strainss. This techn nique is far more conveenient than n rest ...
Human Ig heavy chain CDR3 regions in adult
... especially in humans (7,14–17). The importance of HCDR3 diversity is illustrated by the fact that this region forms the center of the antigen binding site and provides essential residues for antigen binding (2,3,18–22). Given the central location of the HCDR3 within the antigen-binding pocket, major ...
... especially in humans (7,14–17). The importance of HCDR3 diversity is illustrated by the fact that this region forms the center of the antigen binding site and provides essential residues for antigen binding (2,3,18–22). Given the central location of the HCDR3 within the antigen-binding pocket, major ...
Quantitative RT-PCR Platform to Measure Transcript Levels of C and
... reads/ESTs prior to their assembly into transcripts (Mitchell et al. 2007). Another approach is to search ...
... reads/ESTs prior to their assembly into transcripts (Mitchell et al. 2007). Another approach is to search ...
Isr J Chem (2010) - Weizmann Institute of Science
... dynamic tunnel and in eubacteria they emerge into a shelter formed by ribosome-bound trigger-factor which acts as a “chaperone” to prevent aggregation and misfolding. The peptidyl transferase center (PTC) is the primary catalytic core of the ribosome. It is responsible for promoting the chemical cat ...
... dynamic tunnel and in eubacteria they emerge into a shelter formed by ribosome-bound trigger-factor which acts as a “chaperone” to prevent aggregation and misfolding. The peptidyl transferase center (PTC) is the primary catalytic core of the ribosome. It is responsible for promoting the chemical cat ...
Integrating metabolic, transcriptional regulatory and
... ABSTRACT Motivation: The effort to build a whole-cell model requires the development of new modeling approaches, and in particular, the integration of models for different types of processes, each of which may be best described using different representation. Fluxbalance analysis (FBA) has been usef ...
... ABSTRACT Motivation: The effort to build a whole-cell model requires the development of new modeling approaches, and in particular, the integration of models for different types of processes, each of which may be best described using different representation. Fluxbalance analysis (FBA) has been usef ...
Myrick LK, Nakamoto-Kinoshita M, Lindor NM, Warren ST , et al. Fragile X syndrome due to a missense mutation. European Journal of Human Genetics (2014) 22, 1185-1189.
... This gene harbors a CGG-repeat in its 50 untranslated region. In normal individuals, repeat length is polymorphic, with 29–30 repeats being the most common allele.4 In patients with FXS, there is a large expansion of the repeat sequence to over 200 repeats, referred to as the full mutation. Once the ...
... This gene harbors a CGG-repeat in its 50 untranslated region. In normal individuals, repeat length is polymorphic, with 29–30 repeats being the most common allele.4 In patients with FXS, there is a large expansion of the repeat sequence to over 200 repeats, referred to as the full mutation. Once the ...
RESEARCH ARTICLES Characterization of the Long
... selection among 31 amino acid properties in the three lineages of interest. TreeSAAP allows us to identify these property changes and classify them into categories on a gradient from conservative to radical change. Based on data set– specific nucleotide substitution patterns, a neutral model of expe ...
... selection among 31 amino acid properties in the three lineages of interest. TreeSAAP allows us to identify these property changes and classify them into categories on a gradient from conservative to radical change. Based on data set– specific nucleotide substitution patterns, a neutral model of expe ...
Functional Requirements of the Yellow Fever Virus Capsid Protein
... Although it is known that the flavivirus capsid protein is essential for genome packaging and formation of infectious particles, the minimal requirements of the dimeric capsid protein for virus assembly/disassembly have not been characterized. By use of a trans-packaging system that involved packagi ...
... Although it is known that the flavivirus capsid protein is essential for genome packaging and formation of infectious particles, the minimal requirements of the dimeric capsid protein for virus assembly/disassembly have not been characterized. By use of a trans-packaging system that involved packagi ...