Characterization of the first cultured representative of
... the ARB software package (Ludwig et al., 2004). On the basis of the curated guide tree included in the SSU Ref NR 99 database, a comprehensive data set of reference sequences representing major clades of the PVC superphylum were selected. Thereafter, remaining alignment errors revealed by visual ins ...
... the ARB software package (Ludwig et al., 2004). On the basis of the curated guide tree included in the SSU Ref NR 99 database, a comprehensive data set of reference sequences representing major clades of the PVC superphylum were selected. Thereafter, remaining alignment errors revealed by visual ins ...
Evolution of hard proteins in the sauropsid integument in relation to
... Fig. 1 Macroscopic aspect of reptilian scales (A–D) and histology of the epidermis in scales of different reptiles (E–M). (A) Overlapped trunk scale of snake (Natrix natrix). Bar, 0.5 mm. (B) Little overlapping scale of ventral region of midtrunk region of the tuatara (Sphenodon punctatus). Bar, 0.5 ...
... Fig. 1 Macroscopic aspect of reptilian scales (A–D) and histology of the epidermis in scales of different reptiles (E–M). (A) Overlapped trunk scale of snake (Natrix natrix). Bar, 0.5 mm. (B) Little overlapping scale of ventral region of midtrunk region of the tuatara (Sphenodon punctatus). Bar, 0.5 ...
Identification of DNA polymorphism in cultivars using RAPD and AFLP
... restriction of the genomic DNA but this is then followed by selective rounds of PCR amplifications of the restricted fragments. The fragments are amplified by P33 labeled primers designed to the sequence of the restricted site, plus one of the three additional selected nucleotides. Only fragments c ...
... restriction of the genomic DNA but this is then followed by selective rounds of PCR amplifications of the restricted fragments. The fragments are amplified by P33 labeled primers designed to the sequence of the restricted site, plus one of the three additional selected nucleotides. Only fragments c ...
Isolation, cloning and sequence analysis of the lactate
... The enzyme Taq DNA Polymerase was used to amplify the DNA. This polymerase often adds a single deoxyadenosine to the 3’-end of the amplified fragments which makes these products suitable for use for T vector cloning. The reaction mixture contained 5 µl of Taq DNA Polymerase buffer, which is supplied ...
... The enzyme Taq DNA Polymerase was used to amplify the DNA. This polymerase often adds a single deoxyadenosine to the 3’-end of the amplified fragments which makes these products suitable for use for T vector cloning. The reaction mixture contained 5 µl of Taq DNA Polymerase buffer, which is supplied ...
Site-directed mutagenesis of streptococcal plasmin receptor protein
... receptor protein, Plr, from the group A streptococcal strain 64/14 revealed that it is a glyceraldehyde-3-phosphatedehydrogenase and that the plr gene is present on the chromosome as a single copy. This study continues the functional characterization of Plr as a plasmin receptor. Attempts at inserti ...
... receptor protein, Plr, from the group A streptococcal strain 64/14 revealed that it is a glyceraldehyde-3-phosphatedehydrogenase and that the plr gene is present on the chromosome as a single copy. This study continues the functional characterization of Plr as a plasmin receptor. Attempts at inserti ...
Prolonged organ retention and safety of plasmid DNA
... a versatile, inexpensive, and useful nonviral transfection vector.1 A variety of cell types such as monocytes,2 dendritic cells,3 myoblast cells,4 and hepatocytes5 were studied as target cells for PEI-mediated gene transfection. In vivo, PEI was shown to be an efficient transfection vector in severa ...
... a versatile, inexpensive, and useful nonviral transfection vector.1 A variety of cell types such as monocytes,2 dendritic cells,3 myoblast cells,4 and hepatocytes5 were studied as target cells for PEI-mediated gene transfection. In vivo, PEI was shown to be an efficient transfection vector in severa ...
A SOLUBLE RIBONUCLEIC ACID INTERMEDIATE IN PROTEIN
... irreversibly into cY-peptide linkage in protein has been used in our laboratories for a number of years as a measure of protein synthesis. The essential components of this system are the microsomal ribonucleoprotein particles, certain enzymes derived from the soluble protein fraction, adenosine trip ...
... irreversibly into cY-peptide linkage in protein has been used in our laboratories for a number of years as a measure of protein synthesis. The essential components of this system are the microsomal ribonucleoprotein particles, certain enzymes derived from the soluble protein fraction, adenosine trip ...
Nutritional Genomics
... Several pathways were enriched with differentially expressed genes Many pathways related to embryo development and immune system GO terms - Tube development, Immune response, Cytokine binding InterPro motifs - Several terms associated with immunoglobulin motifs KEGG pathway - cytokine-cytokine r ...
... Several pathways were enriched with differentially expressed genes Many pathways related to embryo development and immune system GO terms - Tube development, Immune response, Cytokine binding InterPro motifs - Several terms associated with immunoglobulin motifs KEGG pathway - cytokine-cytokine r ...
and phylogenetic characterization of Shuni virus Genomic
... The M segment of SAE 18/09 was 4351 nt in length, with a 30 UTR of 23 and a 50 UTR of 113 nucleotides (Fig. 1b). Phylogenetic analysis of the M segment sequences of members of the Simbu group demonstrated that SAE 18/09 was most closely related to Schmallenberg virus (SBV), with 61.4 % nucleotide se ...
... The M segment of SAE 18/09 was 4351 nt in length, with a 30 UTR of 23 and a 50 UTR of 113 nucleotides (Fig. 1b). Phylogenetic analysis of the M segment sequences of members of the Simbu group demonstrated that SAE 18/09 was most closely related to Schmallenberg virus (SBV), with 61.4 % nucleotide se ...
Structure of a Plasmodium yoelii gene
... (Maniatis et al. 1982) using a kit from Boehringer and was used as a probe. Unincorporated label was removed by Sephadex G-50 column filtration (Maniatis et al. 1982). Conditions for prehybridization and hybridization were the same as those for Northern blot analysis (see below). For Northern blot a ...
... (Maniatis et al. 1982) using a kit from Boehringer and was used as a probe. Unincorporated label was removed by Sephadex G-50 column filtration (Maniatis et al. 1982). Conditions for prehybridization and hybridization were the same as those for Northern blot analysis (see below). For Northern blot a ...
Global Proteomics of the Extremophile Black Fungus Cryomyces
... carotenoids in colourless and brown-red formations; and 3) the dense cell wall built of chitin, melanin and polysaccharides [9]. All those characteristics make the fungi able to survive diverse stress factors [10]-[12]; they are even known to be survivals of outer space and Mars like environments [1 ...
... carotenoids in colourless and brown-red formations; and 3) the dense cell wall built of chitin, melanin and polysaccharides [9]. All those characteristics make the fungi able to survive diverse stress factors [10]-[12]; they are even known to be survivals of outer space and Mars like environments [1 ...
SCID Screening: A New York State of Mind
... Mutations in the ADA gene reduce or eliminate the activity of the enzyme adenosine deaminase Toxic buildup of deoxyadenosine ensues ...
... Mutations in the ADA gene reduce or eliminate the activity of the enzyme adenosine deaminase Toxic buildup of deoxyadenosine ensues ...
Human mitochondrial leucyl tRNA synthetase can suppress non
... D Synthesis of mitochondrially encoded proteins increases upon aaRS overexpression. Mitochondrially encoded proteins in 143B.206 Rho+, T1V1 and T1L1 cells with and without aaRS overexpression, were pulse labeled with 35S-methionine and separated by 15% SDS–PAGE. Below the autoradiogram is a western ...
... D Synthesis of mitochondrially encoded proteins increases upon aaRS overexpression. Mitochondrially encoded proteins in 143B.206 Rho+, T1V1 and T1L1 cells with and without aaRS overexpression, were pulse labeled with 35S-methionine and separated by 15% SDS–PAGE. Below the autoradiogram is a western ...
Raven/Johnson Biology 8e
... B. Answer b is incorrect. The DNA microarray is only sensitive to the presence or absence of an mRNA, not DNA. The correct answer is c—The pattern of gene expression C. Answer c is correct. DNA microarray technology uses fluorescent probes to examine the pattern of gene expression in various samples ...
... B. Answer b is incorrect. The DNA microarray is only sensitive to the presence or absence of an mRNA, not DNA. The correct answer is c—The pattern of gene expression C. Answer c is correct. DNA microarray technology uses fluorescent probes to examine the pattern of gene expression in various samples ...
MUSCULAR DYSTROPHY, DUCHENNE TYPE
... complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. ...
... complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. ...
Lecture 0 - Introduction - LCQB
... pumps drugs and poisons out of cells recognizes foreign objects ...
... pumps drugs and poisons out of cells recognizes foreign objects ...
Bioinformatics Toolbox
... routines include spatial plots of microarray data (maimage, redgreencmap), box plots (maboxplot), loglog plots (maloglog), and intensity-ratio plots (mairplot). You can also view clustered expression profiles (clustergram, redgreencmap). You can create 2–D scatter plots of principal components from ...
... routines include spatial plots of microarray data (maimage, redgreencmap), box plots (maboxplot), loglog plots (maloglog), and intensity-ratio plots (mairplot). You can also view clustered expression profiles (clustergram, redgreencmap). You can create 2–D scatter plots of principal components from ...
Structural Bioinformatics - LCQB
... pumps drugs and poisons out of cells recognizes foreign objects ...
... pumps drugs and poisons out of cells recognizes foreign objects ...
Molecular modeling of HIV-1 reverse
... A computer model of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) either alone, or complexed with a non-nucleoside inhibitor (NNI), was constructed using crystal coordinate data from a subset of the protein surrounding the binding pocket region. Molecular mechanics calculati ...
... A computer model of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) either alone, or complexed with a non-nucleoside inhibitor (NNI), was constructed using crystal coordinate data from a subset of the protein surrounding the binding pocket region. Molecular mechanics calculati ...
DNA and RNA Extraction Controls Performance Summary
... an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR. Bioline have specially developed a DNA Extraction Control (DEC) and RNA Extraction Control (REC), which more closely mimic the test sample, as compared to sp ...
... an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR. Bioline have specially developed a DNA Extraction Control (DEC) and RNA Extraction Control (REC), which more closely mimic the test sample, as compared to sp ...
Quantitative RT–PCR Platform to Measure Transcript Levels of C
... polyploid nature of wheat genomes, however, makes genetic and functional analyses extremely challenging. As tetraploid durum wheat accounts for only about 5% of global wheat production (Peng et al. 2011), much less effort has been dedicated to developing genomic resources for durum wheat than for he ...
... polyploid nature of wheat genomes, however, makes genetic and functional analyses extremely challenging. As tetraploid durum wheat accounts for only about 5% of global wheat production (Peng et al. 2011), much less effort has been dedicated to developing genomic resources for durum wheat than for he ...
Identification of enzymes involved in anaerobic benzene
... probably encoded by ORFs 95–96 and 98 in culture BF (Table 1). Benzoate specific protein expression. In benzoate-grown cells different putatively bam-related ORFs probably encoding benzoate-CoA ligase (ORF 194; Table 1) and benzoyl-CoA reductase (ORFs 53, 56, 86, 152, 155, 157, 159, 168 and 178; Tab ...
... probably encoded by ORFs 95–96 and 98 in culture BF (Table 1). Benzoate specific protein expression. In benzoate-grown cells different putatively bam-related ORFs probably encoding benzoate-CoA ligase (ORF 194; Table 1) and benzoyl-CoA reductase (ORFs 53, 56, 86, 152, 155, 157, 159, 168 and 178; Tab ...
Supplemental Material
... pSwtRlacZwhiteRz. The choice of these five amino acid residues is based on the published structure of E. coli -gal (JUERS et al. 2001), in which these five amino acid residues seem to contribute to the formation of dimer and tetramer of -gal, which is essential for its activity. We predicted tha ...
... pSwtRlacZwhiteRz. The choice of these five amino acid residues is based on the published structure of E. coli -gal (JUERS et al. 2001), in which these five amino acid residues seem to contribute to the formation of dimer and tetramer of -gal, which is essential for its activity. We predicted tha ...
... mitochondrial dysfunction plays a central role in the pathogenesis of NAFLD, and that NAFLD is a mitochondrial disease (Pessayre and Fromenty, 2005). The liver, as a super metabolic achiever in the body, plays a critical role in metabolism of carbohydrate, protein and fat to maintain blood glucose a ...