... mitochondrial dysfunction plays a central role in the pathogenesis of NAFLD, and that NAFLD is a mitochondrial disease (Pessayre and Fromenty, 2005). The liver, as a super metabolic achiever in the body, plays a critical role in metabolism of carbohydrate, protein and fat to maintain blood glucose a ...
Double muscling in cattle due to mutations in the myostatin gene
... blot analysis, this mutation was found in both alleles in 10y10 double-muscled Piedmontese cattle examined. This mutation is likely to result in a complete or almost complete loss of function, as this cysteine residue is invariant not only among all myostatin sequences but also among all known membe ...
... blot analysis, this mutation was found in both alleles in 10y10 double-muscled Piedmontese cattle examined. This mutation is likely to result in a complete or almost complete loss of function, as this cysteine residue is invariant not only among all myostatin sequences but also among all known membe ...
Cloning and analysis of the mobile element gypsy from D. virilis
... 5'PyPuT/cTGCATAc/TPyPy which is repeated 12 times (8 in some copies of D. melanogaster gypsy, see ref. 5). It seems very likely that this region acts also as an enhancer in D. virilis and binds a product of the same gene, su(Hw). A poly(A) block is also present in both species upstream of the enhanc ...
... 5'PyPuT/cTGCATAc/TPyPy which is repeated 12 times (8 in some copies of D. melanogaster gypsy, see ref. 5). It seems very likely that this region acts also as an enhancer in D. virilis and binds a product of the same gene, su(Hw). A poly(A) block is also present in both species upstream of the enhanc ...
Metabolomic and Gene Expression Profiles Exhibit Modular Genetic
... factors to the mechanism of a complex human disease such as MetS is daunting because of the expense of procuring the enormous sample sizes necessary to make statistically valid conclusions, particularly in the face of tremendous genetic and lifestyle variation. However, it is becoming increasingly e ...
... factors to the mechanism of a complex human disease such as MetS is daunting because of the expense of procuring the enormous sample sizes necessary to make statistically valid conclusions, particularly in the face of tremendous genetic and lifestyle variation. However, it is becoming increasingly e ...
The aconitase of Escherichia cok purification of the
... 10 mM-MgSO,. The cultures were concentrated 20-fold in 10 mMMgS04 for phage adsorption (15 min at room temperature with an m.0.i. of 2) and diluted into 20ml prewarmed LB broth containing 10 m~-MgsO,. The infected bacteria were incubated at 37 "C with shaking, and samples (1.5 ml) were pelleted (150 ...
... 10 mM-MgSO,. The cultures were concentrated 20-fold in 10 mMMgS04 for phage adsorption (15 min at room temperature with an m.0.i. of 2) and diluted into 20ml prewarmed LB broth containing 10 m~-MgsO,. The infected bacteria were incubated at 37 "C with shaking, and samples (1.5 ml) were pelleted (150 ...
Write on zinc fingers
... Cys2His2 The Cys2His2-like fold group is by far the best-characterized class of zinc fingers and is extremely common in mammalian transcription factors. These domains adopt a simple ββα fold and have the amino acid Sequence motif: X2-Cys-X2,4-Cys-X12-His-X3,4,5-His This class of zinc fingers can hav ...
... Cys2His2 The Cys2His2-like fold group is by far the best-characterized class of zinc fingers and is extremely common in mammalian transcription factors. These domains adopt a simple ββα fold and have the amino acid Sequence motif: X2-Cys-X2,4-Cys-X12-His-X3,4,5-His This class of zinc fingers can hav ...
Turnip yellow mosaic virus RNA can be separated into two distinct
... When the TYMV RNA was not heated or treated with SDS before being loaded on the gel, little or no 300 000 molecular weight component could be detected. 3 - Preparation of purified heavy and light RNAs In order to prepare large quantities of the purified light and heavy RNAs we passed heat- and SDS- ...
... When the TYMV RNA was not heated or treated with SDS before being loaded on the gel, little or no 300 000 molecular weight component could be detected. 3 - Preparation of purified heavy and light RNAs In order to prepare large quantities of the purified light and heavy RNAs we passed heat- and SDS- ...
PDF - Fred Hutchinson Cancer Research Center
... rate of 5:6 105 mutations per nucleotide per tissueculture generation—a value similar to that estimated previously by others using a somewhat different experimental approach (Parvin et al. 1986). The rates of different types of mutations are in table 3 and possess expected features such as an ele ...
... rate of 5:6 105 mutations per nucleotide per tissueculture generation—a value similar to that estimated previously by others using a somewhat different experimental approach (Parvin et al. 1986). The rates of different types of mutations are in table 3 and possess expected features such as an ele ...
Effects of Natural Selection on Interpopulation Divergence
... by altering regulation (Kimura and Ohta 1974; Nei 1987). Estimation of gene diversity (heterozygosity) at 1442 SNP sites in an ethnically diverse sample of humans revealed consistently reduced gene diversities at sites where SNPs caused amino acid changes, particularly those predicted to be disrupti ...
... by altering regulation (Kimura and Ohta 1974; Nei 1987). Estimation of gene diversity (heterozygosity) at 1442 SNP sites in an ethnically diverse sample of humans revealed consistently reduced gene diversities at sites where SNPs caused amino acid changes, particularly those predicted to be disrupti ...
Full Text - The International Journal of Developmental Biology
... to the cellular and subcellular levels and, from the 1950’s on, to the molecular level, transformed experimental embryology to developmental biology”. The term “decline” of experimental embryology was falsely interpreted by some authors as a decline of the Freiburg school. That this opinion is wrong ...
... to the cellular and subcellular levels and, from the 1950’s on, to the molecular level, transformed experimental embryology to developmental biology”. The term “decline” of experimental embryology was falsely interpreted by some authors as a decline of the Freiburg school. That this opinion is wrong ...
Figure 4-24, step 1
... RNA polymerase binds and “unwinds” DNA mRNA created from sense strand mRNA is processed by RNA interference ...
... RNA polymerase binds and “unwinds” DNA mRNA created from sense strand mRNA is processed by RNA interference ...
Biology Review
... Primary structure- linear unique sequence of amino acids joined by peptide bonds. The primary structure of a protein is its unique sequence of amino acids Peptide bonds are polar and therefore the C=O of one amino acid can also H bond to the N-H of another amino acid, and a water molecule is formed ...
... Primary structure- linear unique sequence of amino acids joined by peptide bonds. The primary structure of a protein is its unique sequence of amino acids Peptide bonds are polar and therefore the C=O of one amino acid can also H bond to the N-H of another amino acid, and a water molecule is formed ...
A Metabolic Node in Action: Chorismate
... gene is not regulated at the transcriptional level. It is not derepressed by the general amino acid control system, as no consensus for the GCN4 activator protein binding site was found.74 The enzyme is a dimer and its activity is under dual control as it is modulated by a tenfold activation by Trp ...
... gene is not regulated at the transcriptional level. It is not derepressed by the general amino acid control system, as no consensus for the GCN4 activator protein binding site was found.74 The enzyme is a dimer and its activity is under dual control as it is modulated by a tenfold activation by Trp ...
A Brief History of PCR - Bio-Rad
... PCR can identify disease-causing organisms much earlier than other methods, since it looks for the DNA of the organism itself, not its proteins or its effect on our immune system. PCR has even been used to diagnose diseases of the past, by amplifying minute amounts of disease-related DNA in preserve ...
... PCR can identify disease-causing organisms much earlier than other methods, since it looks for the DNA of the organism itself, not its proteins or its effect on our immune system. PCR has even been used to diagnose diseases of the past, by amplifying minute amounts of disease-related DNA in preserve ...
Discovery of Paralogous Nuclear Gene Sequences Coding for the
... DeSalle (1996) suggested that nucleic acid sequences and their translated amino acid codings could be combined to address this problem. We performed combined, as well as separate, amino acid and DNA sequence analyses. Intron sequences were removed from the alignments prior to analysis, and the prese ...
... DeSalle (1996) suggested that nucleic acid sequences and their translated amino acid codings could be combined to address this problem. We performed combined, as well as separate, amino acid and DNA sequence analyses. Intron sequences were removed from the alignments prior to analysis, and the prese ...
lncRNA in
... • Arbitrarily defined as >200bp RNA’s that do not code for protein • Excludes Pseudogenes and Nonsense Mediated Decay products ...
... • Arbitrarily defined as >200bp RNA’s that do not code for protein • Excludes Pseudogenes and Nonsense Mediated Decay products ...
Chicken eggshell matrix proteins related to anti
... 1. The most abundant egg white proteins have been shown to be localized in the eggshell. Ovalbumin is localized in the mammillae of the eggshell (Hincke 1995) and is predominant at the initial stage of eggshell formation. Lysozyme (Hincke et al., 2000) and ovotransferrin (Gautron et al., 2001a) are ...
... 1. The most abundant egg white proteins have been shown to be localized in the eggshell. Ovalbumin is localized in the mammillae of the eggshell (Hincke 1995) and is predominant at the initial stage of eggshell formation. Lysozyme (Hincke et al., 2000) and ovotransferrin (Gautron et al., 2001a) are ...
Three multidomain esterases from the cellulolytic
... More specifically, they differ at residues 10 and 11 within their putative calcium-binding motifs (Fig. 4) ; these two residues have been proposed by Pages et al. (1997) to be particularly important in cohesin-binding selectivity of dockerins from Clostridium spp. The R. flavefaciens dockerins do no ...
... More specifically, they differ at residues 10 and 11 within their putative calcium-binding motifs (Fig. 4) ; these two residues have been proposed by Pages et al. (1997) to be particularly important in cohesin-binding selectivity of dockerins from Clostridium spp. The R. flavefaciens dockerins do no ...
Molecular and Structural Characterization of
... et al., 2000, 2001; Zouhar et al., 2001) and are thought to be essential for the recognition of the aglycone moiety. Furthermore, Ala-467 was demonstrated to make contact with the methoxy group of DIMBOAGlc. While Trp-378 and Phe-198 are conserved in rye at the corresponding positions, Phe-205, Phe- ...
... et al., 2000, 2001; Zouhar et al., 2001) and are thought to be essential for the recognition of the aglycone moiety. Furthermore, Ala-467 was demonstrated to make contact with the methoxy group of DIMBOAGlc. While Trp-378 and Phe-198 are conserved in rye at the corresponding positions, Phe-205, Phe- ...
in Graminaceous Plants
... we noticed that NAAT activity was also present in the fraction passing through the DEAE column from the roots of Fe-deficient barley only. Initially, we thought that the NAAT activity in this fraction was NAAT-I and NAAT-II that failed to bind to the DEAE Sephacel gel. However, after the fraction pa ...
... we noticed that NAAT activity was also present in the fraction passing through the DEAE column from the roots of Fe-deficient barley only. Initially, we thought that the NAAT activity in this fraction was NAAT-I and NAAT-II that failed to bind to the DEAE Sephacel gel. However, after the fraction pa ...
A Pneumocystis carinii multi-gene family with
... 1993). The library was screened with a cDNA clone containing a region of a P. carinii f. sp. carinii MSG gene (EMBL accession number 20870, donated by C. J. Delves and F. Volpe), as part of a study examining subtelomeric sequences in P. carinii. A relatively high number of recombinant plaques gave p ...
... 1993). The library was screened with a cDNA clone containing a region of a P. carinii f. sp. carinii MSG gene (EMBL accession number 20870, donated by C. J. Delves and F. Volpe), as part of a study examining subtelomeric sequences in P. carinii. A relatively high number of recombinant plaques gave p ...