The Diversity of Lysine-Acetylated Proteins in Escherichia coli
... were identified, including enzymes involved in protein synthesis, carbohydrate metabolism, TCA cycle, amino acid and nucleotide biosyntheses, chaperones, transcription, biosynthesis of cellular constituents, detoxification, and energy metabolism (Fig. 2A). Most notably, many protein synthesis and ca ...
... were identified, including enzymes involved in protein synthesis, carbohydrate metabolism, TCA cycle, amino acid and nucleotide biosyntheses, chaperones, transcription, biosynthesis of cellular constituents, detoxification, and energy metabolism (Fig. 2A). Most notably, many protein synthesis and ca ...
PDF - International Journal of Medical Sciences
... were polymorphisms existing in two important antigens, MPT64 (4) and PstS1 (5) in clinical M. tuberculosis strains isolated from China. This may be the reason for changes in the antigens produced, which may in turn cause alteration of related functions, thereby allowing immune evasion. Some other pr ...
... were polymorphisms existing in two important antigens, MPT64 (4) and PstS1 (5) in clinical M. tuberculosis strains isolated from China. This may be the reason for changes in the antigens produced, which may in turn cause alteration of related functions, thereby allowing immune evasion. Some other pr ...
Pattern searches for the identification of putative lipoprotein genes in
... Lpp processing enzymes may be subtly different. One exception within this criterion is the Cu,Zn superoxide dismutase Lpp of Mycobacterium tuberculosis where non-denaturing electrophoresis indicated that the native membrane-associated enzyme activity is electrophoretically identical to that which ha ...
... Lpp processing enzymes may be subtly different. One exception within this criterion is the Cu,Zn superoxide dismutase Lpp of Mycobacterium tuberculosis where non-denaturing electrophoresis indicated that the native membrane-associated enzyme activity is electrophoretically identical to that which ha ...
Achromatopsia caused by novel mutations in both CNGA3 and
... single heterozygous mutations could be found in CNGA3 in three families and in CNGB3 in one family; the most likely explanation for this is that the second mutation is in either an intron or promoter region that is outside the regions sequenced, or an as yet unidentified exon. It is also possible th ...
... single heterozygous mutations could be found in CNGA3 in three families and in CNGB3 in one family; the most likely explanation for this is that the second mutation is in either an intron or promoter region that is outside the regions sequenced, or an as yet unidentified exon. It is also possible th ...
A Method to Identify Protein Sequences that Fold into a Known
... 3D structure profiles. In order to search a sequence database for are subdivided into two types, labeled P, and P, in order of the proteins most compatible with an environment string, we used increasing polarity. Since we treat water as polar, exposed positions the Profile method (25, 26), which was ...
... 3D structure profiles. In order to search a sequence database for are subdivided into two types, labeled P, and P, in order of the proteins most compatible with an environment string, we used increasing polarity. Since we treat water as polar, exposed positions the Profile method (25, 26), which was ...
Stringent Response in Myxococcus xanthus
... Starve for amino acids Fruiting body formation Starve for amino acids Accumulation of (p)ppGpp Accumulate (p)ppGpp ??Starve for aa Fruiting bodies Is this a causal relationship? Is there a connection between initiation of fruiting body development and (p)ppGpp accumulation? ...
... Starve for amino acids Fruiting body formation Starve for amino acids Accumulation of (p)ppGpp Accumulate (p)ppGpp ??Starve for aa Fruiting bodies Is this a causal relationship? Is there a connection between initiation of fruiting body development and (p)ppGpp accumulation? ...
OPTIMIZER: a web server for optimizing the codon usage of DNA
... a codon usage table in a variety of formats, including tables from the Codon Usage Database (9), or they can choose between 153 pre-computed codon usage tables for ribosomal protein genes or a group of highly expressed genes from prokaryotic genomes under translational selection. Users can also choo ...
... a codon usage table in a variety of formats, including tables from the Codon Usage Database (9), or they can choose between 153 pre-computed codon usage tables for ribosomal protein genes or a group of highly expressed genes from prokaryotic genomes under translational selection. Users can also choo ...
On Periodicity in the Occurrence of Nucleotides in Protein Coding
... a genome, which are identified by locating the START and the STOP codons in the genome. There may be overlapping ORFs. To keep things simple, let us consider only prokaryotic genomes so that there are no introns ...
... a genome, which are identified by locating the START and the STOP codons in the genome. There may be overlapping ORFs. To keep things simple, let us consider only prokaryotic genomes so that there are no introns ...
PCR Optimization: Reaction Conditions and
... molecules. For DNA amplification, the optimized reaction buffer (XL Buffer) contains DMSO and glycerol, and is included with both the component product enzyme and complete GeneAmp® XL PCR Kit. PCR Thermal Profiles Using the GeneAmp® PCR System 9700 or 2720 with the two-temperature PCR protocol and ...
... molecules. For DNA amplification, the optimized reaction buffer (XL Buffer) contains DMSO and glycerol, and is included with both the component product enzyme and complete GeneAmp® XL PCR Kit. PCR Thermal Profiles Using the GeneAmp® PCR System 9700 or 2720 with the two-temperature PCR protocol and ...
Exploring Proteins - Weber State University
... • Immunoglobulins (antibodies) can be utilized as powerful analytical tools in biochemistry • An antibody (Immunoglobulin, Ig) is a complex protein formed by an animal in response to the presence of a foreign substance (most often foreign proteins). • An antibody usually exhibits specific and high ...
... • Immunoglobulins (antibodies) can be utilized as powerful analytical tools in biochemistry • An antibody (Immunoglobulin, Ig) is a complex protein formed by an animal in response to the presence of a foreign substance (most often foreign proteins). • An antibody usually exhibits specific and high ...
PINK1 positively regulates HDAC3 to suppress p53
... deacetylase activity. We demonstrate that PINK1 prevents H2O2-induced C-terminal cleavage of HDAC3 by inhibiting caspase-7 activity, which was reversed by protein phosphatase 4c (PP4c). PINK1-mediated phosphorylation of HDAC3 enhances its direct association with p53 and causes subsequent hypoacetyla ...
... deacetylase activity. We demonstrate that PINK1 prevents H2O2-induced C-terminal cleavage of HDAC3 by inhibiting caspase-7 activity, which was reversed by protein phosphatase 4c (PP4c). PINK1-mediated phosphorylation of HDAC3 enhances its direct association with p53 and causes subsequent hypoacetyla ...
Cloning and sequencing of a gene encoding acidophilic amylase
... from cells grown on plates by washing with 0.5 M-Na acetate buffer (pH 5.0) (Boyer et al., 1979). This suggests that the protein is associated with the cytoplasmic membrane or cell wall at least under the growth conditions tested. We also isolated amylase activity by washing cells grown on plates. T ...
... from cells grown on plates by washing with 0.5 M-Na acetate buffer (pH 5.0) (Boyer et al., 1979). This suggests that the protein is associated with the cytoplasmic membrane or cell wall at least under the growth conditions tested. We also isolated amylase activity by washing cells grown on plates. T ...
The Macrophage Colony-Stimulating Factor 1Response Signature
... A reproducible subset of breast carcinomas shows the CSF1 response signature. In each of the five whole tumor breast carcinoma data sets, a subset of similar size (17-25%) of breast carcinomas shows the CSF1 response gene signature (Fig. 1). Cases of breast cancer with the CSF1 response signature we ...
... A reproducible subset of breast carcinomas shows the CSF1 response signature. In each of the five whole tumor breast carcinoma data sets, a subset of similar size (17-25%) of breast carcinomas shows the CSF1 response gene signature (Fig. 1). Cases of breast cancer with the CSF1 response signature we ...
Effect of Six Decades of Selective Breeding on
... To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, deter ...
... To evaluate the extent of the genetic change and its effects on the seed protein composition of soybean cultivars released during the past 60 years, representative ancestral cultivars and those derived from selective breeding were grown in a side-by-side comparison. Total seed protein content, deter ...
Applied Microbiology and Biotechnology
... as methane source, a bacterial consortium is necessary to support growth over longer periods in continuous cultures. In different bioreactors of Norferm Danmark A/S, three bacteria consistently invaded M. capsulatus cultures growing under semi-sterile conditions in continuous culture. These bacteria ...
... as methane source, a bacterial consortium is necessary to support growth over longer periods in continuous cultures. In different bioreactors of Norferm Danmark A/S, three bacteria consistently invaded M. capsulatus cultures growing under semi-sterile conditions in continuous culture. These bacteria ...
University of Groningen Sugar transport in
... Hyperthermophilic archaea show important metabolic adaptations for growth on carbohydrates under hostile conditions. For carbohydrate uptake so far only ABC-type transporters have been described that are equipped with a uniquely high affinity as compared to mesophilic bacterial systems. This allows ...
... Hyperthermophilic archaea show important metabolic adaptations for growth on carbohydrates under hostile conditions. For carbohydrate uptake so far only ABC-type transporters have been described that are equipped with a uniquely high affinity as compared to mesophilic bacterial systems. This allows ...
Genomic analysis and comparison of very virulent infectious bursal
... manipulated in one context and another in vaccines development. Although much interest was given to VP2 alone in vaccines development but it is also found that VP3 and VP4 are equally important. Researchers found that plasmids DNA encoding VP2 initiated a very low level of neutralizing antibody whic ...
... manipulated in one context and another in vaccines development. Although much interest was given to VP2 alone in vaccines development but it is also found that VP3 and VP4 are equally important. Researchers found that plasmids DNA encoding VP2 initiated a very low level of neutralizing antibody whic ...
Platinum DNA polymerases
... Figure 1. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq polymerase. It is difficu ...
... Figure 1. Relative fidelity values of different DNA polymerases. Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq polymerase. It is difficu ...
Managing people in sport organisations
... Copyright © 2009 by Academic Press. All rights reserved. ...
... Copyright © 2009 by Academic Press. All rights reserved. ...
Comparative transcriptomics reveals key gene expression
... chemostat. This revealed that the human and bovine pathogens show differential expression of genes encoding a range of functions, including cell wall and secreted proteins, transcriptional regulators, PE/PPE proteins, lipid metabolism and toxin–antitoxin pairs. Furthermore, we probed the gene expres ...
... chemostat. This revealed that the human and bovine pathogens show differential expression of genes encoding a range of functions, including cell wall and secreted proteins, transcriptional regulators, PE/PPE proteins, lipid metabolism and toxin–antitoxin pairs. Furthermore, we probed the gene expres ...
A tightly regulated and reversibly inducible siRNA expression
... of target gene expression. In addition, several different schemes for both the tet-responsive Tet-repressoroperator- and IPTG-responsive lac repressor-operatormediated inducible shRNA expression suffer from the low levels of background transcription in the non-inducing condition. Therefore, construc ...
... of target gene expression. In addition, several different schemes for both the tet-responsive Tet-repressoroperator- and IPTG-responsive lac repressor-operatormediated inducible shRNA expression suffer from the low levels of background transcription in the non-inducing condition. Therefore, construc ...