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AAV User Manual - Vigene Biosciences
AAV User Manual - Vigene Biosciences

... Combine tissue specific promoters and Cre dependent FLEX-On inducible design can get more restricted tissue specific and temporal expression. In FLEX-ON system, gene is in reverse orientation down stream of promoter, and the gene is flanked by two oppositely orientated loxPs. In the absence of Cr ...
Volume 18 - Number 9 September 2014 Atlas of Genetics and Cytogenetics
Volume 18 - Number 9 September 2014 Atlas of Genetics and Cytogenetics

... contains a proline rich, SH3 domain binding motif, three tyrosine containing SH2 domain binding sites (Xu et al., 2007), of which a YXXM motif is for PI3 kinase subunit p85 binding (Lodyga et al., 2009). In the middle region, there are two pleckstrin homology domains and another tyrosine binding mot ...
Dissecting protein function: an efficient protocol for
Dissecting protein function: an efficient protocol for

... function mutations) can often uncover a previously unknown role for a protein in a particular biological process. However, most mutations are identified based on loss-of-function (LOF) phenotypes, which cannot differentiate between separation-of-function alleles vs. mutations that encode unstable/un ...
DNA markers in plant genome analysis With the advent of molecular
DNA markers in plant genome analysis With the advent of molecular

... genomic library. The genomic libraries are easy to construct and almost all sequence types are included; however, a large number of interspersed repeats are found in inserts, that detect a large number of restriction fragments forming complex patterns. In plants, this problem is overcome to some ext ...
Improving representation of biological responses
Improving representation of biological responses

... Valerie Wood Varsha Khodiyar Stan Laulederkind ...
Answers to Scoring in Scrabble (English Word Play)
Answers to Scoring in Scrabble (English Word Play)

... Lysine rich region could also be a Lysine every other amino acid (KNKNKN) (AA[AG].{3}){4,} the .{3} specifies the intervening codon. To allow some but not all amino acids to occupy this “lysine rich region” we would have to write the regex for each one allowed and separate them with the OR symbol | ...
Odd-paired controls frequency doubling in Drosophila segmentation
Odd-paired controls frequency doubling in Drosophila segmentation

... More recent models of pair-rule patterning recognise that the pair-rule genes form a complex gene regulatory network that mediates dynamic patterns of expression (Edgar et al. 1989; Sánchez & Thieffry 2003; Jaynes & Fujioka 2004). However, whereas other stages of Drosophila segmentation have been ex ...
- Wiley Online Library
- Wiley Online Library

... decade because of its potential applications in agro-industrial processes, such as the pulp and paper industry and biofuel production. These studies have shown that xylan bioconversion is mediated by a wide array of enzymes (Collins et al., 2005; Dodd & Cann, 2009). Although the xylanolytic systems ...
Translation
Translation

Direct binding of Nur77/NAK-1 to the plasminogen activator inhibitor
Direct binding of Nur77/NAK-1 to the plasminogen activator inhibitor

... ⫺250 to ⫺270 ⌬NBRE]) were cloned into a luciferase expression vector, pUBT-luc.36 At 24 hours before transfection, HUVECs were seeded in 6-well tissue-culture plates to reach 80% to 90% confluence the next morning. Transient transfections were performed by using the Lipofectamine Plus reagent (Invit ...
Alignment
Alignment

... • PAM was developed by Margaret Dayhoff and co-workers in 1978 by examining 1572 mutations between 71 families of closely related proteins • BLOSUM is more recent and computed from blocks of sequences with sufficient ...
food produced from glyphosate tolerant sugar beet line 77
food produced from glyphosate tolerant sugar beet line 77

... into sugar beet plants by the addition of two new genes. One of these genes encodes the CP4-EPSPS protein, a key enzyme in the biosynthesis of aromatic amino acids in plants and microbes that is not sensitive to applications of glyphosate. The second gene, the gox gene, encodes the glyphosate oxidor ...
Autotaxin–Lysophosphatidic Acid Axis Acts Downstream of
Autotaxin–Lysophosphatidic Acid Axis Acts Downstream of

... Figure IA and IB in the online-only Data Supplement). After sorting, RNA was extracted and hybridized to an Agilent 4×44 microarray. The data were analyzed to compare between the outcomes of (1) high and normal lipoprotein levels (apoCII versus WT), (2) low and normal lipoprotein levels (stl versus ...
food produced from glyphosate
food produced from glyphosate

... into sugar beet plants by the addition of two new genes. One of these genes encodes the CP4-EPSPS protein, a key enzyme in the biosynthesis of aromatic amino acids in plants and microbes that is not sensitive to applications of glyphosate. The second gene, the gox gene, encodes the glyphosate oxidor ...
Gene Section PKM2 (pyruvate kinase isoenzyme type M2) in Oncology and Haematology
Gene Section PKM2 (pyruvate kinase isoenzyme type M2) in Oncology and Haematology

... the highly active tetrameric form. When FBP levels drop below a critical value the tetrameric form dissociates to the dimeric form. Dimerization of M2PK is induced by direct interaction with different oncoproteins, i.e. pp60v-src, A-Raf and HPV-16 E7. The importance of M2-PK for oncogenesis is furth ...
General Biology I Final Exam
General Biology I Final Exam

... • A fatty acid with one or more double bonds, not fully hydrogenated ...
Synthesis of RNA - Stamm revision
Synthesis of RNA - Stamm revision

... splint oligonucleotide. Could you make a figure for this? The required RNA segments can either all be prepared by chemical synthesis or larger fragments can be generated enzymatically by in vitro transcription using T7 RNA polymerase. Although T7 RNA polymerase accepts certain modified NTPs as subst ...
Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof
Single-Molecule Fluorescence Using Nucleotide Analogs: A Proof

... Table S1 in the Supporting Information). The surfaceimmobilized dsDNAs were first localized by exciting Cy3 with a 532 nm laser, and we monitored its fluorescence until photobleaching (∼40 s) (Figure 2d). Next, we switched to a 325 nm laser to monitor 2AP fluorescence (Figure 2d). Of the >200 dsDNA mol ...
Lactase persistence DNA variant enhances
Lactase persistence DNA variant enhances

... primarily in people of northern European descent and is attributed to inheritance of an unidentified autosomal dominant mutation that prevents the normal maturational decline in lactase expression (6). Enattah et al. (7), using linkage disequilibrium and haplotype analysis in humans, have recently r ...
AN ABSTRACT OF THE THESIS OF Sara R. Fassio for the degree of
AN ABSTRACT OF THE THESIS OF Sara R. Fassio for the degree of

... determinants comprise a network of genes called the ToxT regulon, whose expression is modulated by a hierarchy of transcriptional regulators (17). These regulators include the transcription factors AphA and AphB, which positively regulate transcription of the twocomponent regulatory system tcpPH. Th ...
Oxypred: Prediction and Classification of Oxygen-Binding
Oxypred: Prediction and Classification of Oxygen-Binding

Lactase persistence DNA variant enhances
Lactase persistence DNA variant enhances

... primarily in people of northern European descent and is attributed to inheritance of an unidentified autosomal dominant mutation that prevents the normal maturational decline in lactase expression (6). Enattah et al. (7), using linkage disequilibrium and haplotype analysis in humans, have recently r ...
Histones - scientia.global
Histones - scientia.global

... database and contains a novel 73-aminoacid RNA-binding domain. Professor Marzluff and his group developed antibodies specific for the cloned SLBP, which allowed them to study the function of SLBP. These advances by Professor Marzluff and his team have paved the way for much of the research performed ...
List
List

... the lesion frequency generated by these fingers is 10 to 20% at the optimal dose. UV2 = low expression promoter for omega-ZFP selections The kan gene in the F1/2/3 bbs plasmids generates a 1.2 kb dropout when the plasmids are digested with BbsI. This simplified the purification of double-cut backbon ...
• 100 times faster than dynamic programming. • Good for database
• 100 times faster than dynamic programming. • Good for database

... S= score of High-scoring segment pair (HSP) ...
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Silencer (genetics)

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