A family of diatom-like silicon transporters in the siliceous loricate
... to the S. diplocostata SITs over 193 amino acids. The remaining contigs, mostly shorter than 250 bp, encode protein fragments similar to short stretches within this region of the protein, but the fragmentary genome data precludes determination of how many genes they represent. We conclude that D. gr ...
... to the S. diplocostata SITs over 193 amino acids. The remaining contigs, mostly shorter than 250 bp, encode protein fragments similar to short stretches within this region of the protein, but the fragmentary genome data precludes determination of how many genes they represent. We conclude that D. gr ...
Transcript Fold Change - University of Saskatchewan
... responses in the livers of fathead minnows exposed to: Oil sands process affected water (OSPW). OSPW is generated during the extraction of bitumen form oil sands deposits of Alberta, Canada. It can be toxic to aquatic wildlife, but little is know about the specific mechanisms of toxicity. RNAseq i ...
... responses in the livers of fathead minnows exposed to: Oil sands process affected water (OSPW). OSPW is generated during the extraction of bitumen form oil sands deposits of Alberta, Canada. It can be toxic to aquatic wildlife, but little is know about the specific mechanisms of toxicity. RNAseq i ...
Restriction Digest of pAMP and pKAN
... Hind III and BamH I have digested the original plasmids and that we have the correct restriction fragments. Gel electrophoresis is a procedure commonly used to separate fragments of DNA according to molecular size or number of base pairs. DNA fragments will migrate through the agarose maze. DNA, bec ...
... Hind III and BamH I have digested the original plasmids and that we have the correct restriction fragments. Gel electrophoresis is a procedure commonly used to separate fragments of DNA according to molecular size or number of base pairs. DNA fragments will migrate through the agarose maze. DNA, bec ...
Structure, function and regulation of pyruvate carboxylase
... biotinylation of apoenzyme to holoenzyme, rather than by gene induction. In contrast, changes in PC specific activity of Rhodobacter capsulatus under different growth conditions are mediated at the level of enzyme synthesis [50]. -Aspartate is known to inhibit PC activity and biotinylation in yeast ...
... biotinylation of apoenzyme to holoenzyme, rather than by gene induction. In contrast, changes in PC specific activity of Rhodobacter capsulatus under different growth conditions are mediated at the level of enzyme synthesis [50]. -Aspartate is known to inhibit PC activity and biotinylation in yeast ...
CS273_SequenceSimilarity1
... Still OK? Most changes in functional sites are deleterious and will be rejected ...
... Still OK? Most changes in functional sites are deleterious and will be rejected ...
A novel sensitive method for the detection of user
... Motivation: Most biological sequences contain compositionally biased segments in which one or more residue types are significantly overrepresented. The function and evolution of these segments are poorly understood. Usually, all types of compositionally biased segments are masked and ignored during ...
... Motivation: Most biological sequences contain compositionally biased segments in which one or more residue types are significantly overrepresented. The function and evolution of these segments are poorly understood. Usually, all types of compositionally biased segments are masked and ignored during ...
A Multidrug Resistance–Associated Protein Involved in
... et al., 1999). MRPs use the energy generated by the hydrolysis of MgATP to facilitate the transmembrane movement of a variety of small molecules. Substrates are not limited to glutathionated compounds but include a range of heterocyclic organic anions and heavy metals (Rea et al., 1998). Such compou ...
... et al., 1999). MRPs use the energy generated by the hydrolysis of MgATP to facilitate the transmembrane movement of a variety of small molecules. Substrates are not limited to glutathionated compounds but include a range of heterocyclic organic anions and heavy metals (Rea et al., 1998). Such compou ...
Interaction of DNA with ribosomes in cell-free protein
... culture in inorganic salt medium 10 under continuous illumination at 30 °C. The single steps of the prepara tion of ribosomes and supernatant enzymes have been previously described *. After disruption of the cells with glass beads, the homogenate was precentrifuged 30 min at 30 000 g. From this sup ...
... culture in inorganic salt medium 10 under continuous illumination at 30 °C. The single steps of the prepara tion of ribosomes and supernatant enzymes have been previously described *. After disruption of the cells with glass beads, the homogenate was precentrifuged 30 min at 30 000 g. From this sup ...
Novel DNA Polymerase Increases Efficiency of Multiple PCR
... long-range PCR, LAMP, and PCDR, a recently developed method that was presented in another Biotechniques paper published in February 2013. First, the researchers compared the properties of SD polymerase to Bst polymerase in LAMP, carrying out reactions at 63° C with or without an initial two-minute, ...
... long-range PCR, LAMP, and PCDR, a recently developed method that was presented in another Biotechniques paper published in February 2013. First, the researchers compared the properties of SD polymerase to Bst polymerase in LAMP, carrying out reactions at 63° C with or without an initial two-minute, ...
a server for analyzing and predicting RNA
... protein–RNA complex. The output is a display of the sequence of each chain in the complex, with a label for each residue; ‘þ’ for residues that are within the specified distance cutoff and ‘’ for residues that do not have any atoms within the distance cutoff. The calculated RNA-binding residues are al ...
... protein–RNA complex. The output is a display of the sequence of each chain in the complex, with a label for each residue; ‘þ’ for residues that are within the specified distance cutoff and ‘’ for residues that do not have any atoms within the distance cutoff. The calculated RNA-binding residues are al ...
TFE3 contains two activation domains, one acidic and the other
... suggesting that these regions are important for function. Using two different assays, we show that the Inland C-terminal activation domains of TFE3 act synergistically. This synergism explains in part the ability of TFE3S to act as a dominant negative. Our domain analysis of TFE3 is incorporated int ...
... suggesting that these regions are important for function. Using two different assays, we show that the Inland C-terminal activation domains of TFE3 act synergistically. This synergism explains in part the ability of TFE3S to act as a dominant negative. Our domain analysis of TFE3 is incorporated int ...
... amino acid Gly. If Gly is attached, then it is removed by hydrolysis at a separate editing site on the enzyme. Based on the structure of these amino acids, provide a sketch or description of the site which adds the amino acid to the tRNA and the separate site that will remove Gly but not Ala from th ...
Isolation of Vibrio harveyi Acyl Carrier Protein and the fabG, acpP
... protein 78% identical to E. coli 3-ketoacyl-ACP reductase (21); acpP (nucleotides 1282 to 1512; see below); and fabF (nucleotides 1609 to 2853), encoding a 43.1-kDa protein 76% identical to E. coli 3-ketoacyl-ACP synthase II. The latter protein was originally reported as 3-ketoacyl-ACP synthase IV, ...
... protein 78% identical to E. coli 3-ketoacyl-ACP reductase (21); acpP (nucleotides 1282 to 1512; see below); and fabF (nucleotides 1609 to 2853), encoding a 43.1-kDa protein 76% identical to E. coli 3-ketoacyl-ACP synthase II. The latter protein was originally reported as 3-ketoacyl-ACP synthase IV, ...
The Old World monkey DAZ (Deleted in
... The Y-chromosomal cynDAZ cDNA The cynDAZ cDNA sequence (Fig. 1) is a composite of the partial cDNA of the remaining fifth clone and a 5′-RACE product using adult monkey testis mRNA as a template. The 5′-RACE products overlapped the cDNA insert by 50 nucleotides and extended 471 nucleotides further t ...
... The Y-chromosomal cynDAZ cDNA The cynDAZ cDNA sequence (Fig. 1) is a composite of the partial cDNA of the remaining fifth clone and a 5′-RACE product using adult monkey testis mRNA as a template. The 5′-RACE products overlapped the cDNA insert by 50 nucleotides and extended 471 nucleotides further t ...
Cloning and Functional Characterization of a Phospholipid
... a Glu aligning with this position but instead has Ala or Ser, which have smaller side chains. Lipases, as well as HsLCAT, contain a so-called lid domain which is closed by a disulfide bridge. The lid structure occurs between the two Cys, C74 and C98, at the N- and C- terminal parts of the segment in ...
... a Glu aligning with this position but instead has Ala or Ser, which have smaller side chains. Lipases, as well as HsLCAT, contain a so-called lid domain which is closed by a disulfide bridge. The lid structure occurs between the two Cys, C74 and C98, at the N- and C- terminal parts of the segment in ...
Alpha and beta subunits of pyruvate dehydrogenase E1
... within a clade composed exclusively of eukaryotic mitochondrial PDH genes (with bootstrap support between 70 and 97%), which is itself related to the alpha-proteobacterial PDH clade (bootstrap support between 95 and 100%). This position for the microsporidian sequence is exactly what one would expec ...
... within a clade composed exclusively of eukaryotic mitochondrial PDH genes (with bootstrap support between 70 and 97%), which is itself related to the alpha-proteobacterial PDH clade (bootstrap support between 95 and 100%). This position for the microsporidian sequence is exactly what one would expec ...
The protein acetylome and the regulation of metabolism - Serval
... For example acetylation is required for tropomyosin binding to actin, it modifies the kinetic properties of the rat glycine N-methyltransferase, causes the loss of specific peptidase activities of 20S proteosome, modifies the interaction strength between the E2 enzyme Ubiquitin Conjugating 12 (Ubc12 ...
... For example acetylation is required for tropomyosin binding to actin, it modifies the kinetic properties of the rat glycine N-methyltransferase, causes the loss of specific peptidase activities of 20S proteosome, modifies the interaction strength between the E2 enzyme Ubiquitin Conjugating 12 (Ubc12 ...
περισσότερες πληροφορίες
... plasmid DNA in bacteria - such as E. coli - a process which occurs in nature, albeit seldom. • Transformation is achieved by modifying some chemicalphysical properties of the bacterial membrane using chemical substances (CaCl2) associated with rapid heat shock, or an electric shock at high voltage ( ...
... plasmid DNA in bacteria - such as E. coli - a process which occurs in nature, albeit seldom. • Transformation is achieved by modifying some chemicalphysical properties of the bacterial membrane using chemical substances (CaCl2) associated with rapid heat shock, or an electric shock at high voltage ( ...
Computational Biology - Bioinformatik
... - double-stranded RNA molecules, - that are 20-25 nucleotides in length (often precisely 21 nt) and play a variety of roles in biology. Most notably, siRNA is involved in the RNA interference (RNAi) pathway, where it interferes with the expression of a specific gene. In addition to their role in the ...
... - double-stranded RNA molecules, - that are 20-25 nucleotides in length (often precisely 21 nt) and play a variety of roles in biology. Most notably, siRNA is involved in the RNA interference (RNAi) pathway, where it interferes with the expression of a specific gene. In addition to their role in the ...
Origins of Life PDF
... to form ribonucleotides (a chemical composed of a ribose, a nucleobase, and a phosphate). In time, perhaps aided by mineral or clay catalysts, ribonucleotides assembled into long chain polymers to form RNAs of varying sequences (see Figure 2, next page). These diverse RNAs accumulated on the planet, ...
... to form ribonucleotides (a chemical composed of a ribose, a nucleobase, and a phosphate). In time, perhaps aided by mineral or clay catalysts, ribonucleotides assembled into long chain polymers to form RNAs of varying sequences (see Figure 2, next page). These diverse RNAs accumulated on the planet, ...
