The maize ID1 flowering time regulator is a zinc finger protein with
... domain. Unexpectedly, site-directed mutagenesis of the ID1 protein showed that zinc ®ngers located at each end of the ID domain are not required for binding to the consensus motif despite the fact that one of these zinc ®ngers is a canonical C2H2 DNA binding domain. In addition, an ID1 in vitro dele ...
... domain. Unexpectedly, site-directed mutagenesis of the ID1 protein showed that zinc ®ngers located at each end of the ID domain are not required for binding to the consensus motif despite the fact that one of these zinc ®ngers is a canonical C2H2 DNA binding domain. In addition, an ID1 in vitro dele ...
The Basics of RT-PCR
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
Drug Development
... • Absorption - Passes GI track into blood stream • Distribution - Gets to target tissue (blood brain barrier) • Metabolism – Not readily metabolized • Excretion – Not readily secreted • Toxicity – Not toxic to other cells or tissues ...
... • Absorption - Passes GI track into blood stream • Distribution - Gets to target tissue (blood brain barrier) • Metabolism – Not readily metabolized • Excretion – Not readily secreted • Toxicity – Not toxic to other cells or tissues ...
Materials and Methods
... whether some sequence parameters have a differential influence across the range of observed expression and solubility score values. A parameter can potentially show an equivalent effect across the entire score range; in this case, an increase in the parameter would have the same influence on the pro ...
... whether some sequence parameters have a differential influence across the range of observed expression and solubility score values. A parameter can potentially show an equivalent effect across the entire score range; in this case, an increase in the parameter would have the same influence on the pro ...
Finding of a novel fungal immunomodulatory protein coding
... De novo transcriptome assembly and bioinformatics analyses Sequenced reads were assembled using the bioinfomatics algorithms employed by the Newbler 2.6 assembler (Roche®). In order to identify mRNA transcripts related to the expression of immunomodulatory proteins in G. australe mycelium, a local B ...
... De novo transcriptome assembly and bioinformatics analyses Sequenced reads were assembled using the bioinfomatics algorithms employed by the Newbler 2.6 assembler (Roche®). In order to identify mRNA transcripts related to the expression of immunomodulatory proteins in G. australe mycelium, a local B ...
The Predicted Candidates of Arabidopsis Plastid Inner Envelope
... make use of certain features of plastid-targeted proteins. The plastid genome itself encodes only about 120 single-copy genes and, therefore, must rely on proteins from the nucleus. The protein constituents that are encoded in the nuclear genome are synthesized in the cytoplasm as higher Mr precurso ...
... make use of certain features of plastid-targeted proteins. The plastid genome itself encodes only about 120 single-copy genes and, therefore, must rely on proteins from the nucleus. The protein constituents that are encoded in the nuclear genome are synthesized in the cytoplasm as higher Mr precurso ...
Comparative genomic analysis of carbon and nitrogen assimilation
... strain. We identified canonical forms of Rubisco encoded in the genomes of both Acidithiobacillus species inspected. In A. ferrooxidans DSM 16786, two gene copies encoding Rubisco form I (cbbSL1 and cbbSL2) and one copy encoding Rubisco form II (cbbM) were identified, as described previously [28], w ...
... strain. We identified canonical forms of Rubisco encoded in the genomes of both Acidithiobacillus species inspected. In A. ferrooxidans DSM 16786, two gene copies encoding Rubisco form I (cbbSL1 and cbbSL2) and one copy encoding Rubisco form II (cbbM) were identified, as described previously [28], w ...
Towards an Analysis of the Rice Mitochondrial Proteome
... Purified rice (Oryza sativa) mitochondrial proteins have been arrayed by isoelectric focusing/polyacrylamide gel electrophoresis (PAGE), by blue-native (BN) PAGE, and by reverse-phase high-performance liquid chromatography (LC) separation (LC-mass spectrometry [MS]). From these protein arrays, we ha ...
... Purified rice (Oryza sativa) mitochondrial proteins have been arrayed by isoelectric focusing/polyacrylamide gel electrophoresis (PAGE), by blue-native (BN) PAGE, and by reverse-phase high-performance liquid chromatography (LC) separation (LC-mass spectrometry [MS]). From these protein arrays, we ha ...
VegT initiates endoderm formation
... transcription factor VegT do not express the endodermal genes, endodermin, Xsox17α, Xlhbox8, insulin and Ifabp, suggesting that VegT plays a role in endoderm formation (Zhang et al., 1998). However, the effect of depleting VegT mRNA on early endoderm formation was not examined. Several other approac ...
... transcription factor VegT do not express the endodermal genes, endodermin, Xsox17α, Xlhbox8, insulin and Ifabp, suggesting that VegT plays a role in endoderm formation (Zhang et al., 1998). However, the effect of depleting VegT mRNA on early endoderm formation was not examined. Several other approac ...
Time-Resolved Transcriptome Analysis of Bacillus subtilis
... of amino-acids and nucleotides, transcriptional regulation, flagellar motility, chemotaxis, phage proteins, sporulation, and many genes of unknown function. Different amino acid treatments were compared in terms of both the global temporal profiles and the 5-minute quick regulations, and between-exp ...
... of amino-acids and nucleotides, transcriptional regulation, flagellar motility, chemotaxis, phage proteins, sporulation, and many genes of unknown function. Different amino acid treatments were compared in terms of both the global temporal profiles and the 5-minute quick regulations, and between-exp ...
The Primary Structure of a 4.0-kDa Photosystem I Polypeptide
... Enzymatic cleavage of the PSI-I polypeptide with pepsin was Photosystem I (PS I)’ in plants and cyanobacteriacatalyzes the photochemical transfer of electrons from plastocyanin to carried out by dissolving the lyophilized PSI-I polypeptide (5 nmol) in 100 p l of 0.2% acetic acid (adjusted to pH 2.0 ...
... Enzymatic cleavage of the PSI-I polypeptide with pepsin was Photosystem I (PS I)’ in plants and cyanobacteriacatalyzes the photochemical transfer of electrons from plastocyanin to carried out by dissolving the lyophilized PSI-I polypeptide (5 nmol) in 100 p l of 0.2% acetic acid (adjusted to pH 2.0 ...
Drought response in the spikes of barley: gene expression in the
... constant variance from block to block. The significance of differential gene expression in stressed and non-stressed organs was determined using the F statistic. To control false discoveries due to multiple hypotheses testing, we used the q value method described by Storey and Tibshirani (2003). Pro ...
... constant variance from block to block. The significance of differential gene expression in stressed and non-stressed organs was determined using the F statistic. To control false discoveries due to multiple hypotheses testing, we used the q value method described by Storey and Tibshirani (2003). Pro ...
The novel genome organization of the insect picorna
... 6420–8969 (ORF-2). One more ORF (greater than 10 kDa) was identified, nucleotides 2225–2602, which is contained within the sequence encoding ORF-1 but in the 1 frame. ORF-1 and ORF-2 account for 86 % of the DCV genome ; the other 14 % consists of non-coding or untranslated regions. These include th ...
... 6420–8969 (ORF-2). One more ORF (greater than 10 kDa) was identified, nucleotides 2225–2602, which is contained within the sequence encoding ORF-1 but in the 1 frame. ORF-1 and ORF-2 account for 86 % of the DCV genome ; the other 14 % consists of non-coding or untranslated regions. These include th ...
Biosynthesis of branched-chain amino acids is
... Sanjuán-Pinilla et al., 2002; Truchet et al., 1980). This is often generally the case for auxotrophic mutants, because the compounds that cannot be synthesized by the rhizobium might also not be provided by the plant in the rhizosphere or during the nodulation process. The symbiotic defect can ofte ...
... Sanjuán-Pinilla et al., 2002; Truchet et al., 1980). This is often generally the case for auxotrophic mutants, because the compounds that cannot be synthesized by the rhizobium might also not be provided by the plant in the rhizosphere or during the nodulation process. The symbiotic defect can ofte ...
A Simple Introduction to NCBI BLAST
... sequence (Figure 6a). Looking at the descriptions, it appears that our unknown D. yakuba genomic DNA has sequence homology to the gene legless in D. melanogaster. Comparing the descriptions for the top eight hits, we noticed that only the D. melanogaster hit has an accession number that begins with ...
... sequence (Figure 6a). Looking at the descriptions, it appears that our unknown D. yakuba genomic DNA has sequence homology to the gene legless in D. melanogaster. Comparing the descriptions for the top eight hits, we noticed that only the D. melanogaster hit has an accession number that begins with ...
April Fools Paper slide
... Single nucleotide mismatches between the crRNA and target sequence abolishes DNA cleavage • Cleavage efficiency was tested with an array of crRNAs with a single base mismatch from the target • Mismatches up to 11 bp 5’ of the PAM site abolished cleavage • Mismatches farther upstream retained effici ...
... Single nucleotide mismatches between the crRNA and target sequence abolishes DNA cleavage • Cleavage efficiency was tested with an array of crRNAs with a single base mismatch from the target • Mismatches up to 11 bp 5’ of the PAM site abolished cleavage • Mismatches farther upstream retained effici ...
Biotechnology and Genetic Engineering
... genes responsible for disease development, by insertion, alteration, or removal of genes within an individual’s cells. – In most gene therapy studies, a normal gene is inserted into the genome to replace an abnormal, disease-causing gene. ...
... genes responsible for disease development, by insertion, alteration, or removal of genes within an individual’s cells. – In most gene therapy studies, a normal gene is inserted into the genome to replace an abnormal, disease-causing gene. ...
DNA copy number analysis by MAPH: molecular diagnostic
... Fragile X syndrome but negative for the FMR1 repeat. In 83 normal individuals, three putative polymorphic changes were found (a deletion at XpYp and duplications at 1q and XqYq), which shows that MAPH can detect and give accurate estimates for the allele frequency of uncommon variants in the populat ...
... Fragile X syndrome but negative for the FMR1 repeat. In 83 normal individuals, three putative polymorphic changes were found (a deletion at XpYp and duplications at 1q and XqYq), which shows that MAPH can detect and give accurate estimates for the allele frequency of uncommon variants in the populat ...
Evolutionary molecular genetic clocks—a perpetual exercise in
... almost exclusively to the functionally terms ‘evolutionary’ + ‘molecular’ + ‘clock’. nearly neutral changes.”16 The high evolutionary rates reported by Zuckerkandl and Pauling paleontologist. They were two of the chief architects of the along with their ideas about amino acid substitution rates so-c ...
... almost exclusively to the functionally terms ‘evolutionary’ + ‘molecular’ + ‘clock’. nearly neutral changes.”16 The high evolutionary rates reported by Zuckerkandl and Pauling paleontologist. They were two of the chief architects of the along with their ideas about amino acid substitution rates so-c ...
Table S2
... Pds1: Inhibits the onset of anaphase by binding and sequestering the Esp1 protease that cleaves the cohesin complexes that hold sister chromatids together. Binding of Pds1 to Esp1 was reported to depend in Cdc28 phosphorylation[53] Sic1: Inhibitor of Clb-Cdc28. Phosphorylation of Sic1 by Cln-Cdc28 a ...
... Pds1: Inhibits the onset of anaphase by binding and sequestering the Esp1 protease that cleaves the cohesin complexes that hold sister chromatids together. Binding of Pds1 to Esp1 was reported to depend in Cdc28 phosphorylation[53] Sic1: Inhibitor of Clb-Cdc28. Phosphorylation of Sic1 by Cln-Cdc28 a ...
PDF
... determination and analysis of long DNA sequences. To analyse these data we have written a number of programs for a Cromemco Z80 based microcomputer. some of which are illustrated in Figs. 1 and 2. In this paper we would like to focus on the level of analysis that occurs prior to the running of seque ...
... determination and analysis of long DNA sequences. To analyse these data we have written a number of programs for a Cromemco Z80 based microcomputer. some of which are illustrated in Figs. 1 and 2. In this paper we would like to focus on the level of analysis that occurs prior to the running of seque ...