NEAT / HyperNEAT
... Historical Markings: Keep a global counter; every time a neuron or synapse is added, assign the value of the counter, and increment it. Genes/synapses can be disabled, but remain in the genome. ...
... Historical Markings: Keep a global counter; every time a neuron or synapse is added, assign the value of the counter, and increment it. Genes/synapses can be disabled, but remain in the genome. ...
Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates
... In bacteria, the ND Bacillus subtilis GluRS has been extensively studied (9). Due to the lack of a canonical glutaminyltRNA synthetase (GlnRS) in this organism, the ND-GluRS is an essential enzyme in Gln-tRNA formation as it generates GlutRNAGln. This product is then converted to Gln-tRNAGln by Glu- ...
... In bacteria, the ND Bacillus subtilis GluRS has been extensively studied (9). Due to the lack of a canonical glutaminyltRNA synthetase (GlnRS) in this organism, the ND-GluRS is an essential enzyme in Gln-tRNA formation as it generates GlutRNAGln. This product is then converted to Gln-tRNAGln by Glu- ...
Diapositive 1 - LBGI Bioinformatique et Génomique Intégratives
... Actin Related Proteins (ARPs) are key players in major biological processes important for cell life. In cytoskeleton activities, the ARP2/3 complex is essential for actin dynamics, ARP1 and ARP11 are involved in microtubule based vesicle trafficking, in nuclear functions (transcriptional activation, ...
... Actin Related Proteins (ARPs) are key players in major biological processes important for cell life. In cytoskeleton activities, the ARP2/3 complex is essential for actin dynamics, ARP1 and ARP11 are involved in microtubule based vesicle trafficking, in nuclear functions (transcriptional activation, ...
The mapping of linear B-cell epitope regions in desmoglein 1 and 3
... recombinant protein fragments [29]. Bhol et al. identified two antigenic protein fragments BOS1 (aa50-79) and BOS6 (aa200-229) from Dgs3 protein sequence [18] with the usage of 12 synthetic 30mer peptides nearly covering the Dsg3 aa50-468 region. Dworschak et al. analyzed 254 14mer peptides from the ...
... recombinant protein fragments [29]. Bhol et al. identified two antigenic protein fragments BOS1 (aa50-79) and BOS6 (aa200-229) from Dgs3 protein sequence [18] with the usage of 12 synthetic 30mer peptides nearly covering the Dsg3 aa50-468 region. Dworschak et al. analyzed 254 14mer peptides from the ...
Gene Expression
... results. Real-time PCR assays with β2-Microglobulin (β2M) were performed in seven tenfold serial dilutions of the same in-vitro transcribed human RNA. Each assay was performed in triplicate on a LightCycler® 2.0 Instrument. The LightCycler® FastStart DNA MasterPLUS Hybridization Probes was used for ...
... results. Real-time PCR assays with β2-Microglobulin (β2M) were performed in seven tenfold serial dilutions of the same in-vitro transcribed human RNA. Each assay was performed in triplicate on a LightCycler® 2.0 Instrument. The LightCycler® FastStart DNA MasterPLUS Hybridization Probes was used for ...
Increased Functional Half-life of Fibroblast Growth Factor
... developmental and metabolic processes. The 22 members of the human FGF family have a conserved ~120 amino acid core, however, they exhibit significant difference in size (17-25kDa) and sequence (13-71% sequence similarity) (Itoh and Ornitz 2004, 2008). Despite of the variations, three positions are ...
... developmental and metabolic processes. The 22 members of the human FGF family have a conserved ~120 amino acid core, however, they exhibit significant difference in size (17-25kDa) and sequence (13-71% sequence similarity) (Itoh and Ornitz 2004, 2008). Despite of the variations, three positions are ...
Gene Section SRXN1 (sulfiredoxin 1) Atlas of Genetics and Cytogenetics
... In adult, Srx protein was found in internal organs such as mouse liver and kidney. Expression pattern of Srx in embryonic development is not clear. Transcriptional regulation of Srx expression is mainly mediated through AP-1 and/or Nrf-2 activation (Jeong et al., 2012). In yeast, it may also be nega ...
... In adult, Srx protein was found in internal organs such as mouse liver and kidney. Expression pattern of Srx in embryonic development is not clear. Transcriptional regulation of Srx expression is mainly mediated through AP-1 and/or Nrf-2 activation (Jeong et al., 2012). In yeast, it may also be nega ...
Fatty acid transport proteins: a current view of a
... downregulated in the adipose tissue of ob/ob mice, which have increased adipogenesis44. Other negative regulators of FATPs include endotoxin, tumor necrosis factor α and interleukin 1, which can mediate a dramatic reduction of hamster liver FATP1 expression45. One caveat is that these studies are ba ...
... downregulated in the adipose tissue of ob/ob mice, which have increased adipogenesis44. Other negative regulators of FATPs include endotoxin, tumor necrosis factor α and interleukin 1, which can mediate a dramatic reduction of hamster liver FATP1 expression45. One caveat is that these studies are ba ...
Going from where to why—interpretable
... subcellular localization that addresses these issues. Due to its simple architecture, YLoc can identify the relevant features of a protein sequence contributing to its subcellular localization, e.g. localization signals or motifs relevant to protein sorting. We present several example applications w ...
... subcellular localization that addresses these issues. Due to its simple architecture, YLoc can identify the relevant features of a protein sequence contributing to its subcellular localization, e.g. localization signals or motifs relevant to protein sorting. We present several example applications w ...
Sequence Alignment - Mainlab Bioinformatics
... Discontiguous MEGABLAST is better at finding nucleotide sequences similar, but not identical, to your nucleotide query. This program uses non-contiguous word within a longer window of template. In coding mode, the third base wobbling is taken into consideration by focusing on finding matches at the ...
... Discontiguous MEGABLAST is better at finding nucleotide sequences similar, but not identical, to your nucleotide query. This program uses non-contiguous word within a longer window of template. In coding mode, the third base wobbling is taken into consideration by focusing on finding matches at the ...
2013_BJ_SCFBio_Website
... *Desired: A sensitivity & specificity of unity (all true genes are predicted with no false positives). While, the above methods have improved over the years and it is remarkable that they perform so well with limited experimental data to train on, more research, new methods transferable across speci ...
... *Desired: A sensitivity & specificity of unity (all true genes are predicted with no false positives). While, the above methods have improved over the years and it is remarkable that they perform so well with limited experimental data to train on, more research, new methods transferable across speci ...
Maxime - Tech Dragon Limited
... Note : The PCR process is covered by patents issued and applicable in certain countries. iNtRON Biotechnology does not encourage or support the unauthorized or unlicensed use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not requi ...
... Note : The PCR process is covered by patents issued and applicable in certain countries. iNtRON Biotechnology does not encourage or support the unauthorized or unlicensed use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not requi ...
Spore Formation Bacillus subtilis during Compartmentalization of
... focus primarily on B. subtilis but include discussion of other species where we think it appropriate. We discuss in detail the genetic and biochemical experiments that have led to the discovery and characterization of cell-specific programs of gene expression. Since sporulation follows a distinct se ...
... focus primarily on B. subtilis but include discussion of other species where we think it appropriate. We discuss in detail the genetic and biochemical experiments that have led to the discovery and characterization of cell-specific programs of gene expression. Since sporulation follows a distinct se ...
Optimizing cofactor availability for the production of recombinant
... the point of induction until the end of cultivation. Considering the complex and so far poorly understood regulation of heme biosynthesis, a multitude of single or combined factors may have either positive or negative effects on this pathway. Thus, additional studies will be required to unravel the ...
... the point of induction until the end of cultivation. Considering the complex and so far poorly understood regulation of heme biosynthesis, a multitude of single or combined factors may have either positive or negative effects on this pathway. Thus, additional studies will be required to unravel the ...
Transcriptomic Footprints Disclose Specificity of Reactive Oxygen
... peroxidases (APXs), catalases (CATs), glutathione peroxidases, and peroxiredoxins are localized within these subcellular compartments (Mittler et al., 2004). The main reasons why the proposed selective signaling by specific ROS remained difficult to tackle experimentally were because until recently, ...
... peroxidases (APXs), catalases (CATs), glutathione peroxidases, and peroxiredoxins are localized within these subcellular compartments (Mittler et al., 2004). The main reasons why the proposed selective signaling by specific ROS remained difficult to tackle experimentally were because until recently, ...
Tetrahydrobiopterin and its functions
... The latter function of BH4 seems to have a dual nature, depending on the concentration of the cofactor and the cell type, between proliferative activity and trigger for apoptosis. Since BH4 (and related tetrahydropterins) can form radicals, it can act as a generator or scavenger of reactive oxygen s ...
... The latter function of BH4 seems to have a dual nature, depending on the concentration of the cofactor and the cell type, between proliferative activity and trigger for apoptosis. Since BH4 (and related tetrahydropterins) can form radicals, it can act as a generator or scavenger of reactive oxygen s ...
(I). Forward mutation: The change from wild-type to mutant
... (III). Dominant negative mutations (I). Loss of function mutations: Wild type alleles typically encode a product necessary for a specific biological function. If a mutation occurs in that allele, the function for which it encodes is also lost. The general term for these mutations is loss-of-func ...
... (III). Dominant negative mutations (I). Loss of function mutations: Wild type alleles typically encode a product necessary for a specific biological function. If a mutation occurs in that allele, the function for which it encodes is also lost. The general term for these mutations is loss-of-func ...
Expressway™ Cell-Free E. coli Expression System
... Successful use of the Expressway™ Cell-Free E. coli Expression System to synthesize recombinant protein requires the addition of a DNA template containing the gene of interest placed within the proper context of transcription and translation regulatory elements including a bacteriophage T7 RNA polym ...
... Successful use of the Expressway™ Cell-Free E. coli Expression System to synthesize recombinant protein requires the addition of a DNA template containing the gene of interest placed within the proper context of transcription and translation regulatory elements including a bacteriophage T7 RNA polym ...
JNK1 plays an important part in this process provides an
... archaeal H/ACA RNA and the Cbf5–Nop10 dimer [5]. Moreover, the PUA domain is essential for RNP assembly and activity because (in the presence of substrate RNA) it supports both processes, even when added in trans [6]. By using the coordinates of the TruB–tRNA complex and a previously solved complex ...
... archaeal H/ACA RNA and the Cbf5–Nop10 dimer [5]. Moreover, the PUA domain is essential for RNP assembly and activity because (in the presence of substrate RNA) it supports both processes, even when added in trans [6]. By using the coordinates of the TruB–tRNA complex and a previously solved complex ...
Transcriptome analysis of the two unrelated fungal
... Aspergillus species. As mentioned above, only one wild-type strain of A. chrysogenum is currently available, which was isolated from Sardinian coastal seawater. Interestingly, the most closely-related fungus carrying pcbC found in our analysis is the marine fungus K. tethys [40]. The first and thus ...
... Aspergillus species. As mentioned above, only one wild-type strain of A. chrysogenum is currently available, which was isolated from Sardinian coastal seawater. Interestingly, the most closely-related fungus carrying pcbC found in our analysis is the marine fungus K. tethys [40]. The first and thus ...
NIH Public Access
... which is the model acetogenic bacterium that has been widely used for elucidating the WoodLjungdahl pathway of CO and CO2 fixation. This pathway, which is also known as the reductive acetyl-CoA pathway, allows acetogenic (often called homoacetogenic) bacteria to convert glucose stoichiometrically in ...
... which is the model acetogenic bacterium that has been widely used for elucidating the WoodLjungdahl pathway of CO and CO2 fixation. This pathway, which is also known as the reductive acetyl-CoA pathway, allows acetogenic (often called homoacetogenic) bacteria to convert glucose stoichiometrically in ...
Full-Text PDF
... Currently, it is still in most cases impossible to purify and describe all of the major and minor allergens of natural allergen sources that are active in aqueous buffers. Thanks to the advance in the field of molecular genetics, this problem can be overcome by using cDNAs for recombinant production ...
... Currently, it is still in most cases impossible to purify and describe all of the major and minor allergens of natural allergen sources that are active in aqueous buffers. Thanks to the advance in the field of molecular genetics, this problem can be overcome by using cDNAs for recombinant production ...
... a) lac operator: binds lac repressor, used to control production of mRNA b) start codon: Initiates protein synthesis. c) ribosome binding site: Binds mRNA to ribosome (30 s subunit) d) stop codon: Terminates protein synthesis. e) mRNA termination: Terminates mRNA synthesis, recruiting rho factor. f) ...