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Protein Tyrosine Nitration
Protein Tyrosine Nitration

... Specific proteins modified by nitration have been detected in more than 50 human disorders Associated with oxidative stress, most of the nitrating agents require the formation of reactive nitrogen and oxygen species Localized at site(s) of injury and in selective cell types Only a selective numb ...
RNA-binding proteins and RNA metabolism: a new scenario in the
RNA-binding proteins and RNA metabolism: a new scenario in the

... up-regulated, respectively, and both miRNAs were capable of binding directly to TDP-43. These TDP43-miRNA interactions have been also shown to affect the expression levels of candidate cellular transcripts, such as the cyclin-dependent kinase 6 (Cdk6), which was previously found to be up-regulated f ...
Severe factor XI deficiency caused by a Gly555 to Glu mutation
Severe factor XI deficiency caused by a Gly555 to Glu mutation

... normal FXI. On SDS–PAGE, recombinant FXIWT and FXI-Glu555 run as 160 kDa proteins unreduced and 80 kDa reduced (Fig. 1b), consistent with the homodimeric structure of plasma FXI [1]. Plasma FXI and FXIWT have comparable specific activities in aPTT assays (200 U mg)1 and 180 U mg)1, respectively); how ...
Pathways to abscisic acid-regulated gene expression
Pathways to abscisic acid-regulated gene expression

... similar to the amino-termini of ARF1, a transcription factor that binds to auxin response elements (Ulmasov et al., 1997), the ARF1-like MONOPTEROS gene involved in embryo axis formation and vascular development (Table 1) (Hardtke & Berleth, 1998), and several Arabidopsis DNA-binding proteins of unk ...
Restriction Digest of pAMP and pKAN
Restriction Digest of pAMP and pKAN

... You might have predicted that when loaded into the electrophoresis gel, K- and A- would produce only a single DNA band; there’s no reason why you would think otherwise. However, it is likely that two or three bands will appear in the undigested K- and A- plasmid lanes. This is because plasmids isol ...
e Study of RNA Polymerase Pausing by Optical Traps
e Study of RNA Polymerase Pausing by Optical Traps

... duration. The cell implements different pausing mechanisms based on the conditions and nutrient requirements in the cell. For example, in Escherichia coli, pausing might couple transcription and translation by synchronizing the interaction between RNA synthesis and ribosome movement in the coding po ...
Characterisation of the novel proteins expressed in corn line 1507
Characterisation of the novel proteins expressed in corn line 1507

... Southwestern corn borer (Diatraea grandiosella), black cutworm (Agrotis ipsilon) and armyworms (Spodoptera sp.). These insects are common pests of corn in the United States where it is intended for this variety to be grown commercially. The Cry1F protein is encoded by the cry1F gene derived from Bac ...
The Molecular Genetics of Bacteriophage P22.
The Molecular Genetics of Bacteriophage P22.

... MIT, Cambridge, 1974). The early genes of P22 are organized into two operons which flank the c2 repressor gene (15, 16). Transcription of each of these operons starts at a promoter/operator complex near the c2 gene and proceeds in the direction away from the c2 gene. The transcription of these opero ...
IOSR Journal of Computer Engineering (IOSR-JCE)
IOSR Journal of Computer Engineering (IOSR-JCE)

... protein contains a unique amino acid sequence which has a set of generic codes called „codon‟. ‟Codon‟ is a set of three nucleotide as for example AUG is a code that contains adenine-uracil-guanine nucleotides respectively. On the other hand, DNA sequence contains four nucleotides such as adenine (A ...
Monocyte chemoattrac- tant protein
Monocyte chemoattrac- tant protein

... It remains controversial whether blockade of the MCP-1 and CCR-2 signaling pathway influences Th cell polarization. MCP-1 can stimulate IL-4 production (17) and its overexpression is associated with defects in cell-mediated immunity (18). Gu et al. demonstrated that lymph node cells from MCP-1defici ...
pcr
pcr

... the DNA polymerase used; Taq polymerase has its optimum activity temperature at 75-80°C, and commonly a temperature of 72°C is used with this enzyme. • At this step the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding dNTP's that are complementary to the ...
The Arabidopsis stearoyl-acyl carrier protein-desaturase
The Arabidopsis stearoyl-acyl carrier protein-desaturase

... pathogens, the host plants initiate one or more signal ...
Anaerobic degradation of aromatic amino acids by
Anaerobic degradation of aromatic amino acids by

... qRT-PCR. Primer pairs used for qRT-PCR analysis of gene transcript ...
History and Function
History and Function

Supplementary Materials
Supplementary Materials

... package termed iPAGE (for information-theoretic Pathway Analysis of Gene Expression3). We compiled gene-sets based on GO annotations4, transcription factor regulons5 and known stress response pathways6. These modules are available in the Supplementary Information. In short, iPAGE calculates the mutu ...
Engineering of factors determining alpha-amylase and
Engineering of factors determining alpha-amylase and

... All A-amylases studied have a small residue at this position (Gly, Leu, Ser, Thr or Val), in contrast to CGTases which have a more bulky aromatic residue (Tyr or Phe) at this position, which is highly conserved. Characterization of the F196G mutant CGTase of T. thermosulfurigenes EM1 revealed that, ...
Snorks Lab File
Snorks Lab File

... protein molecules and that this is virtually the same mechanism for all life forms. ...
using the crispr/cas9 gene editing tool to develop a cure for
using the crispr/cas9 gene editing tool to develop a cure for

... population of mice with Duchenne muscular dystrophy. To do so, Long used the germline technique, whereby he crossed a female with two copies of the mutated DMD gene with a male housing the mutated DMD gene on its only X chromosome, thus ensuring that the parents would produce offspring with DMD. He ...
Discovery of genes in the Pacific oyster (Crassostrea gigas) involved
Discovery of genes in the Pacific oyster (Crassostrea gigas) involved

... 50 mL non-sterile of seawater. Sixteen oysters held in 8 L of seawater were inoculated via a 3 hour immersion bath with V. vulnificus at an initial concentration of 4.56x1019 CFU/L. Control oysters (n=16) were likewise placed in 8L of seawater. Following exposure, oysters were harvested aseptically ...
Mechanisms of Unidirectional Translocation & Unwinding
Mechanisms of Unidirectional Translocation & Unwinding

in a patient with Ambras syndrome
in a patient with Ambras syndrome

Metalloenzyme Functions
Metalloenzyme Functions

... The database of Clusters of Orthologous Groups of proteins (COGs), which represents an attempt on a phylogenetic classification of the proteins encoded in complete genomes, currently consists of 2791 COGs including 45 350 proteins from 30 genomes of bacteria, archaea and the yeast Saccharomyces cere ...
Regulation of Arabidopsis 14-3
Regulation of Arabidopsis 14-3

... amino acids glutamate, glycine, glutamine and γ-aminobutyric acid (GABA), have wellestablished roles as neurotransmitters in the mammalian central nervous system (Reimer et al. 2001). It has been suggested that some of these nitrogen-containing molecules, including glutamate, glycine and GABA may al ...
Analysis - The Journal of Cell Biology
Analysis - The Journal of Cell Biology

Role of Streptococcus sanguinis sortase A in bacterial
Role of Streptococcus sanguinis sortase A in bacterial

... 10557. Lane 4: S. pyogenes strain SSI-1. Strain SSI-1 is known to express the Fn-binding protein FbaB [16]. ...
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Silencer (genetics)

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