Conservation and relative importance of residues across protein
... consequently have different evolutionary pressure for their conservation. The degree of conservation of each interface residue can be defined in terms of sequence entropy at that position in the polypeptide chain across all of the homologous proteins. At least three types of contacts can be identifi ...
... consequently have different evolutionary pressure for their conservation. The degree of conservation of each interface residue can be defined in terms of sequence entropy at that position in the polypeptide chain across all of the homologous proteins. At least three types of contacts can be identifi ...
Accurate and Comprehensive Mapping of Multi-omic Data
... Pathway analysis provides a useful biological context for differentially expressed entities resulting from the analysis of high-throughput data in any ‘omics’ (for example genomics, transcriptomics, proteomics, or metabolomics) experiment. Pathways overrepresented or enriched in the entities of inte ...
... Pathway analysis provides a useful biological context for differentially expressed entities resulting from the analysis of high-throughput data in any ‘omics’ (for example genomics, transcriptomics, proteomics, or metabolomics) experiment. Pathways overrepresented or enriched in the entities of inte ...
Analyses of 16S rRNA and RuBisCO large subunit genes from an
... and nontronite deposit are formed by aerobic oxidation of sulphide, ferrous and other ions which may favor the dominance of the aerobic RuBisCO form I. This explanation is supported by the observation that all the Loihi 16S rDNA OTUs were affiliated to the aerobic species of Proteobacteria. Another ...
... and nontronite deposit are formed by aerobic oxidation of sulphide, ferrous and other ions which may favor the dominance of the aerobic RuBisCO form I. This explanation is supported by the observation that all the Loihi 16S rDNA OTUs were affiliated to the aerobic species of Proteobacteria. Another ...
Molecular and Phylogenetic Characterization of Cytokine Genes
... Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA Abstract: Bats (order Chiroptera) represent nearly one fourth of the approximately ...
... Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA Abstract: Bats (order Chiroptera) represent nearly one fourth of the approximately ...
Effects of Protein-Deprivation on the Regeneration of Rat Liver after
... dietary groups. Samples of liver were immediately frozen in liquid N2 to prevent endogenous protein degradation. The amino acids were categorized for the purpose of discussion into non-essential, essential and urea-cycle intermediates and substrates (Table 4). The hepatic pools of most non-essential ...
... dietary groups. Samples of liver were immediately frozen in liquid N2 to prevent endogenous protein degradation. The amino acids were categorized for the purpose of discussion into non-essential, essential and urea-cycle intermediates and substrates (Table 4). The hepatic pools of most non-essential ...
Biochemical Oscillations
... Cooperative binding of ligands to a multisubunit enzyme is not limited to substrates. Other small molecules may bind to the enzyme and alter its catalytic properties (either its affinity for substrates or its rate of converting bound substrates into products). Such enzymes are called “allosteric” bec ...
... Cooperative binding of ligands to a multisubunit enzyme is not limited to substrates. Other small molecules may bind to the enzyme and alter its catalytic properties (either its affinity for substrates or its rate of converting bound substrates into products). Such enzymes are called “allosteric” bec ...
Paraoxonase-2 Gene (PON2) G148 Variant Associated with
... contrast to PON1, which is mainly expressed in the liver, PON2 is expressed in a variety of tissues, including the pancreas (8). Although the physiological role of the PON2 gene product is unknown, its tissue distribution suggests a role that unique from that of paraoxonase (8). The PDK4 product mod ...
... contrast to PON1, which is mainly expressed in the liver, PON2 is expressed in a variety of tissues, including the pancreas (8). Although the physiological role of the PON2 gene product is unknown, its tissue distribution suggests a role that unique from that of paraoxonase (8). The PDK4 product mod ...
Analyzing human variation with Galaxy
... Part 1: Filtering out SNPs found in genomes of healthy individuals Uploading files Using Galaxy libraries Basic filtering ...
... Part 1: Filtering out SNPs found in genomes of healthy individuals Uploading files Using Galaxy libraries Basic filtering ...
Cloning and Molecular Analysis of the Plasma ... Paramecium tetraurelia
... known genes [ 10, 11, 361 and additional isoforms which are the result of alternative RNA splicing [lo, 11, 361. Members of the gene family have been cloned from a variety of tissues of higher organisms including: human erythrocytes, teratoma cells, intestine, smooth muscles of rabbit and pig, and r ...
... known genes [ 10, 11, 361 and additional isoforms which are the result of alternative RNA splicing [lo, 11, 361. Members of the gene family have been cloned from a variety of tissues of higher organisms including: human erythrocytes, teratoma cells, intestine, smooth muscles of rabbit and pig, and r ...
Selective and specific cleavage of the D 1 and D2 proteins of
... [13], the mobility shifts and aggregation resulted from the actions of active oxygen species (see Refs. [25.26]). No fragments of the 47-kDa protein were detected in each of the three different treatments by immunoblotting with anti47 (data not shown). If the marked mobility shifts of protein bands ...
... [13], the mobility shifts and aggregation resulted from the actions of active oxygen species (see Refs. [25.26]). No fragments of the 47-kDa protein were detected in each of the three different treatments by immunoblotting with anti47 (data not shown). If the marked mobility shifts of protein bands ...
GEFs: master regulators of G
... studies of Ras-directed GEFs is instructive for our understanding of these mechanisms8. There are several distinct families of Ras-GEF and, although all members of these families contain cdc25 domains, each is responsive to different sets of cellular inputs. SOS, for example, contains an SH3 domain, ...
... studies of Ras-directed GEFs is instructive for our understanding of these mechanisms8. There are several distinct families of Ras-GEF and, although all members of these families contain cdc25 domains, each is responsive to different sets of cellular inputs. SOS, for example, contains an SH3 domain, ...
University of Groningen Stress responses and sugar metabolism in
... In addition to these phosphoproteins also low molecular weight molecules such as NADP, glucose‐6‐phosphate (G6P) and fructose‐1,6‐bisphosphate (FBP) can modulate either DNA‐ binding properties or the interaction with the transcriptional machinery of the CcpA‐(HPr‐ Ser ...
... In addition to these phosphoproteins also low molecular weight molecules such as NADP, glucose‐6‐phosphate (G6P) and fructose‐1,6‐bisphosphate (FBP) can modulate either DNA‐ binding properties or the interaction with the transcriptional machinery of the CcpA‐(HPr‐ Ser ...
SV 96 Total RNA Isolation System Technical Bulletin
... 3.A. Direct Purification of RNA The successful isolation of intact RNA requires four steps: effective disruption of cells or tissue, denaturation of nucleoprotein complexes, inactivation of endo-genous ribonuclease (RNase) activity, and removal of contaminating DNA and protein. The most important s ...
... 3.A. Direct Purification of RNA The successful isolation of intact RNA requires four steps: effective disruption of cells or tissue, denaturation of nucleoprotein complexes, inactivation of endo-genous ribonuclease (RNase) activity, and removal of contaminating DNA and protein. The most important s ...
Cell-Free Phospholipid Biosynthesis by Gene
... and implementation of complex genetic and metabolic pathways that can support essential cellular functions. Here, we show that phospholipid biosynthesis, a multiple-step process involved in cell membrane homeostasis, can be reconstituted starting from the genes encoding for all necessary proteins. A ...
... and implementation of complex genetic and metabolic pathways that can support essential cellular functions. Here, we show that phospholipid biosynthesis, a multiple-step process involved in cell membrane homeostasis, can be reconstituted starting from the genes encoding for all necessary proteins. A ...
Dynamic changes of yak (Bos grunniens) gut microbiota during
... alignment length, and finally we identified a total of 6754 KEGG orthologous groups (KOs). Classifications of ...
... alignment length, and finally we identified a total of 6754 KEGG orthologous groups (KOs). Classifications of ...
MARKER GENE TRANSFER TO WINTER DURUM WHEAT (Triticum
... transformation studies. For the tested gold particles, 1.6 µm average diameter was found to give better results if used at 650 psi bombardment pressure and at 6 cm target distance. However 1.0 µm average diameter gold particles was found to be more effective at 1100 psi bombardment pressures at 6 cm ...
... transformation studies. For the tested gold particles, 1.6 µm average diameter was found to give better results if used at 650 psi bombardment pressure and at 6 cm target distance. However 1.0 µm average diameter gold particles was found to be more effective at 1100 psi bombardment pressures at 6 cm ...
MÉu b×a luËn v¨n th¹c sÜ cã in ch÷ nhò khæ 210x297mm
... Avian influenza (AI) is a highly contagious disease cause by type A influenza virus, a member of the Orthomyxoviridae family. Influenza A virus causes major and frequently fatal in birds, as well as in mammals including humans. At times of influenza A virus subtype H5N1 outbreaks, control of further ...
... Avian influenza (AI) is a highly contagious disease cause by type A influenza virus, a member of the Orthomyxoviridae family. Influenza A virus causes major and frequently fatal in birds, as well as in mammals including humans. At times of influenza A virus subtype H5N1 outbreaks, control of further ...
Quantitative analysis of the effect of the mutation frequency on the
... and then were incubated for 45 min. The cells were pelleted by centrifugation and were resuspended in PBS to yield a FACS throughput rate of ⬇2,000 events兾sec. For flow cytometric analyses, the cells were analyzed 5–10 min after resuspension. Each library was subjected to four or five rounds of sele ...
... and then were incubated for 45 min. The cells were pelleted by centrifugation and were resuspended in PBS to yield a FACS throughput rate of ⬇2,000 events兾sec. For flow cytometric analyses, the cells were analyzed 5–10 min after resuspension. Each library was subjected to four or five rounds of sele ...
The Organellar Genome and Metabolic Potential
... approach, the major part of this genome (41,666 bp) has been sequenced and reconstructed as a single contig. This part of the organellar genome contains an almost complete set of genes found in the mitochondrial genomes of other ciliates (table 1, fig. 1). In addition, we found a stretch larger than ...
... approach, the major part of this genome (41,666 bp) has been sequenced and reconstructed as a single contig. This part of the organellar genome contains an almost complete set of genes found in the mitochondrial genomes of other ciliates (table 1, fig. 1). In addition, we found a stretch larger than ...
proposal for complex variants
... According to the current HGVS recommendations, a single nucleotide substitution in the gene conversion and intronic insertion examples above would have to be described using the accession.version number of the sequence submitted to GenBank. Please note that the purpose of the extended description is ...
... According to the current HGVS recommendations, a single nucleotide substitution in the gene conversion and intronic insertion examples above would have to be described using the accession.version number of the sequence submitted to GenBank. Please note that the purpose of the extended description is ...
Alteration by site-directed mutagenesis of the
... This plasmid (pFS-X) was then digested with Xhol and ligated to the isolated mutagenized recB fragment, to reconstruct the original pFS 11-04, except now containing the mutation in the recB gene. The orientation of the inserted fragment was checked by digesting the resulting plasmid (pFS-BK29Q) with ...
... This plasmid (pFS-X) was then digested with Xhol and ligated to the isolated mutagenized recB fragment, to reconstruct the original pFS 11-04, except now containing the mutation in the recB gene. The orientation of the inserted fragment was checked by digesting the resulting plasmid (pFS-BK29Q) with ...
translation and protein structure
... to each other. The tendency for hydrophilic water molecules to interact with each other and for hydrophobic molecules to interact with each other is the very same tendency that leads to the formation of oil droplets in water. This is also the reason why most hydrophobic amino acids tend to be buried ...
... to each other. The tendency for hydrophilic water molecules to interact with each other and for hydrophobic molecules to interact with each other is the very same tendency that leads to the formation of oil droplets in water. This is also the reason why most hydrophobic amino acids tend to be buried ...