Patents and Biotechnology
... DNA is a double-stranded helix composed of complementary nucleic acid basepairs: adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G). DNA consists of sequences of nucleic acids, such as ACTTAGGAC. Proteins are composed of amino acids, which can fold to make complex structures ...
... DNA is a double-stranded helix composed of complementary nucleic acid basepairs: adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G). DNA consists of sequences of nucleic acids, such as ACTTAGGAC. Proteins are composed of amino acids, which can fold to make complex structures ...
Chpt14_Translation.doc
... 1. Actively translating proteins were labeled with radioactive amino acids for a brief time (short relative to the time required to complete synthesis). 2. Completed polypeptides were collected, digested with trypsin, and the amount of radioactivity in tryptic fragments was determined. 3. Tryptic fr ...
... 1. Actively translating proteins were labeled with radioactive amino acids for a brief time (short relative to the time required to complete synthesis). 2. Completed polypeptides were collected, digested with trypsin, and the amount of radioactivity in tryptic fragments was determined. 3. Tryptic fr ...
Functional characterization of dosage‐dependent lethal mutation of
... this strain caused cell lysis even under normal conditions. SUB62, being a wild-type strain for UBI4, by contrast remained unaffected by UbEP42 expression (Prabha et al., 2010). These results suggested that UbEP42 seemed to obstruct the process of protein degradation in SUB60 cells either by hamperi ...
... this strain caused cell lysis even under normal conditions. SUB62, being a wild-type strain for UBI4, by contrast remained unaffected by UbEP42 expression (Prabha et al., 2010). These results suggested that UbEP42 seemed to obstruct the process of protein degradation in SUB60 cells either by hamperi ...
HotStarTaq® Plus DNA Polymerase and Master Mix and
... minimizing the risk of contamination. Lengthy calculations are avoided and setting up amplification reactions is fast and easy. Since errors due to pipetting variability and miscalculations are reduced, PCR results are highly reproducible. In addition, the hot start provided by HotStarTaq Plus DNA P ...
... minimizing the risk of contamination. Lengthy calculations are avoided and setting up amplification reactions is fast and easy. Since errors due to pipetting variability and miscalculations are reduced, PCR results are highly reproducible. In addition, the hot start provided by HotStarTaq Plus DNA P ...
- Wiley Online Library
... of information about the bacterial genome. The public databases now contain the sequences of billions of nucleotides coding for entire genomes, as well as for individual structural and functional genes, while the protein databases contain the amino acid sequences of the polypeptides encoded by many ...
... of information about the bacterial genome. The public databases now contain the sequences of billions of nucleotides coding for entire genomes, as well as for individual structural and functional genes, while the protein databases contain the amino acid sequences of the polypeptides encoded by many ...
EPISTASIS & METABOLISM Presented by Chintan Joshi
... Conclusions 1. Interactions observed for Synechocystis and E. coli under aerobic growth for glucose are similar. ...
... Conclusions 1. Interactions observed for Synechocystis and E. coli under aerobic growth for glucose are similar. ...
Full-Text PDF
... amounts of foreign DNA have made the herpesviruses a promising target for the development of gene therapy and vaccine vectors. For these purposes, replication-limited herpesvirus vectors have been developed through the deletion of an essential viral gene, which can subsequently be provided in trans ...
... amounts of foreign DNA have made the herpesviruses a promising target for the development of gene therapy and vaccine vectors. For these purposes, replication-limited herpesvirus vectors have been developed through the deletion of an essential viral gene, which can subsequently be provided in trans ...
Isolated Spinach Ribulose-1,5-Bisphosphate
... transgenic tobacco plants,” Proc. Nat. Acad. Sci. USA 89:8394—8398 (1992)). These studies have been inconclu ...
... transgenic tobacco plants,” Proc. Nat. Acad. Sci. USA 89:8394—8398 (1992)). These studies have been inconclu ...
Protein-protein interaction networks (I): data preprocessing
... happen that they never find close to each other because they could be localized in different cell types or could be expressed at different points. (ii) False-negatives: Interactions that where unidentified in the experiment. If two proteins interact outside the nucleus, they may not do so inside the ...
... happen that they never find close to each other because they could be localized in different cell types or could be expressed at different points. (ii) False-negatives: Interactions that where unidentified in the experiment. If two proteins interact outside the nucleus, they may not do so inside the ...
CH Zinc Fingers As DNA Binding Domains
... fingers in that it requires an additional, non-zinc finger domain to establish the binding to the target DNA.^^ The other classes, that is the triple, multiple-adjacent and separated-paired C2H2 fingers, bind to the specific DNA sequence without the aid of other domains. Both the triple C2H2 and the ...
... fingers in that it requires an additional, non-zinc finger domain to establish the binding to the target DNA.^^ The other classes, that is the triple, multiple-adjacent and separated-paired C2H2 fingers, bind to the specific DNA sequence without the aid of other domains. Both the triple C2H2 and the ...
Invited Chapter One
... Of the three subfamilies that include only nematode proteins two contain a single sequence and the third contains three proteins. Even though they signal for a DPP/BMP subfamily member and they are clearly R-Smads, SMA-2 and SMA-3 are different enough from other R-Smads (and each other) that they ea ...
... Of the three subfamilies that include only nematode proteins two contain a single sequence and the third contains three proteins. Even though they signal for a DPP/BMP subfamily member and they are clearly R-Smads, SMA-2 and SMA-3 are different enough from other R-Smads (and each other) that they ea ...
Innovative Purification Protocol for Heparin Binding
... size that enable it to fit the intended substrate very snugly [17]. It also has the correct chemical properties to bind the substrate efficiently. The active site also contains certain amino acids that are involved in the chemical reaction catalyzed by the enzyme [18]. Although not all proteins are ...
... size that enable it to fit the intended substrate very snugly [17]. It also has the correct chemical properties to bind the substrate efficiently. The active site also contains certain amino acids that are involved in the chemical reaction catalyzed by the enzyme [18]. Although not all proteins are ...
Supplementary material: Additional file 1 Chavali
... and these codes were derived from the DrugBank database. Drugs can have more than one code (e.g. aspirin) and some share the same code(s) (e.g. phenothiazine derivative drugs – promethazine and trimeprazine). Taking into account multiple yet distinct second‐leve ...
... and these codes were derived from the DrugBank database. Drugs can have more than one code (e.g. aspirin) and some share the same code(s) (e.g. phenothiazine derivative drugs – promethazine and trimeprazine). Taking into account multiple yet distinct second‐leve ...
doc - Environmental Protection Authority
... GM strains may or may not be lost during subsequent breeding, depending on the viability and mating success of the mice containing them. In addition, some genetic modifications are spontaneously lost or do not express themselves (for a variety of reasons) so that the modifications may not be retaine ...
... GM strains may or may not be lost during subsequent breeding, depending on the viability and mating success of the mice containing them. In addition, some genetic modifications are spontaneously lost or do not express themselves (for a variety of reasons) so that the modifications may not be retaine ...
EPICENTRE Enzyme Catalog
... Polymerase is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity f ...
... Polymerase is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity f ...
Supplementary Information (doc 3773K)
... Subcellular fractionation analysis showed that Drosha is mainly increased in the nucleus of EWS KO (-/-) cells. The protein levels of DGCR8 and Dicer were not changed. Lamin B, nucleus marker; UBF, nucleolar marker; and eIF3η, cytosolic marker. ...
... Subcellular fractionation analysis showed that Drosha is mainly increased in the nucleus of EWS KO (-/-) cells. The protein levels of DGCR8 and Dicer were not changed. Lamin B, nucleus marker; UBF, nucleolar marker; and eIF3η, cytosolic marker. ...
Protein kinases of the human malaria parasite Plasmodium
... • the calmodulin-dependent kinases (CaMK), and • the tyrosine kinases (TyrK). ePKs that did not clearly fit into any of these groups were placed into the OPK ("other protein kinases") group. The primary structure of all enzymes in these groups conform to the model described by Hanks, in which the ca ...
... • the calmodulin-dependent kinases (CaMK), and • the tyrosine kinases (TyrK). ePKs that did not clearly fit into any of these groups were placed into the OPK ("other protein kinases") group. The primary structure of all enzymes in these groups conform to the model described by Hanks, in which the ca ...
Cra-mediated regulation of Escherichia coli adenylate cyclase
... of many genes (Saier & Ramseier, 1996). cAMP is also essential for transcriptional regulation of many genes via its receptor protein CRP. When complexed with CAMP, CRP binds to specific sites upstream of promoters, causing transcriptional activation or repression (Kolb et al., 1993). A CRP binding s ...
... of many genes (Saier & Ramseier, 1996). cAMP is also essential for transcriptional regulation of many genes via its receptor protein CRP. When complexed with CAMP, CRP binds to specific sites upstream of promoters, causing transcriptional activation or repression (Kolb et al., 1993). A CRP binding s ...
2. Large-scale Metabolic Reconstruction
... especially during the stage of manual curation. For the mouse genome-scale metabolic model, however, we were able to rapidly reconstruct a compartmentalized model from well-curated metabolic databases online. The prototype model was comprehensive. Apart from minor compound naming and compartmentaliz ...
... especially during the stage of manual curation. For the mouse genome-scale metabolic model, however, we were able to rapidly reconstruct a compartmentalized model from well-curated metabolic databases online. The prototype model was comprehensive. Apart from minor compound naming and compartmentaliz ...
Identification of fungal oxaloacetate hydrolyase within the
... oxidation of glyoxylate7 and glycolaldehyde.8 The acetoacetate/OAH route predominates in oxalate producers examined to date1,9–14 and therefore the oah gene might be used for the classification of fungi as potential oxalate producers. Recently, the genomes of a large number of oxalate and nonoxalate ...
... oxidation of glyoxylate7 and glycolaldehyde.8 The acetoacetate/OAH route predominates in oxalate producers examined to date1,9–14 and therefore the oah gene might be used for the classification of fungi as potential oxalate producers. Recently, the genomes of a large number of oxalate and nonoxalate ...
CMBI
... – How do the proteins encoded in genomes interact with each other to produce cells and phenotypes ? – To predict such functional interactions between proteins as there exist e.g. in metabolic pathways, signalling pathways or protein complexes ...
... – How do the proteins encoded in genomes interact with each other to produce cells and phenotypes ? – To predict such functional interactions between proteins as there exist e.g. in metabolic pathways, signalling pathways or protein complexes ...
Analysis of similarity of the S1 gene in infectious bronchitis virus (IBV
... Coronaviruses have the largest RNA virus genomes and replicate by means of the specific enveloped transcription. Moreover, its mismatching percent and the combination percent is very high, so the virus can mutate easily (Moore et al 1998). Thus the viruses’ antigenicity and pathogenicity are changed ...
... Coronaviruses have the largest RNA virus genomes and replicate by means of the specific enveloped transcription. Moreover, its mismatching percent and the combination percent is very high, so the virus can mutate easily (Moore et al 1998). Thus the viruses’ antigenicity and pathogenicity are changed ...
Tools Enabling Metabolic Parents LEarning
... Humans have chromosomes composed of DNA. Genes are pieces of DNA that carry the genetic instruction. Each chromosome may have several thousand genes. The word mutation means a change or error in the genetic instruction. We inherit particular chromosomes from the egg of the mother and sperm of the fa ...
... Humans have chromosomes composed of DNA. Genes are pieces of DNA that carry the genetic instruction. Each chromosome may have several thousand genes. The word mutation means a change or error in the genetic instruction. We inherit particular chromosomes from the egg of the mother and sperm of the fa ...