PhD Thesis - Cox Group
... Polyketides are formed by a similar biosynthetic pathway to that of fatty acids. They are formed by repetitive decarboxylative condensation of simple acetate units in the form of acetyl CoA 24 and malonyl CoA 25 (in some cases propionate and butyrate units) in a head to tail manner. In polyketide p ...
... Polyketides are formed by a similar biosynthetic pathway to that of fatty acids. They are formed by repetitive decarboxylative condensation of simple acetate units in the form of acetyl CoA 24 and malonyl CoA 25 (in some cases propionate and butyrate units) in a head to tail manner. In polyketide p ...
Functional characterization of polypeptide release factor 1b in the
... UAA/UGA through Ser-Pro-Phe (SPF) [4,5]. In higher eukaryotes, there is only one RF-I, eRF1 (eukaryotic release factor 1), which can decode all three stop codons [6–8]. ...
... UAA/UGA through Ser-Pro-Phe (SPF) [4,5]. In higher eukaryotes, there is only one RF-I, eRF1 (eukaryotic release factor 1), which can decode all three stop codons [6–8]. ...
Channel-mediated lactic acid transport: a novel function for
... proteins, together with EcAqpZ, EcGlpF, and human Aqp0 as an outgroup, were aligned with ClustalX2 [32] and a phylogenetic tree constructed using the neighbour-joining method [33]. Five distinct phylogenetic groups were deduced from this alignment (Figure 1A). In order to infer a potential role of e ...
... proteins, together with EcAqpZ, EcGlpF, and human Aqp0 as an outgroup, were aligned with ClustalX2 [32] and a phylogenetic tree constructed using the neighbour-joining method [33]. Five distinct phylogenetic groups were deduced from this alignment (Figure 1A). In order to infer a potential role of e ...
From Sequence to Function
... other during evolution (see section 1-1). Most of the commonly used alignment programs give each aligned pair of amino acids a score based on the likelihood of that particular match occurring. These scores are usually obtained from reference tables of the observed frequencies of particular substitut ...
... other during evolution (see section 1-1). Most of the commonly used alignment programs give each aligned pair of amino acids a score based on the likelihood of that particular match occurring. These scores are usually obtained from reference tables of the observed frequencies of particular substitut ...
Molecular insights into RNA and DNA helicase evolution from the
... molecule called RNA. DNA consists of two strands that are wound around each other in a double helix. RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a three-dimensional structure that is held in shape by regions of the molecule interacting with each ...
... molecule called RNA. DNA consists of two strands that are wound around each other in a double helix. RNA is made in a similar way to DNA, but it is usually present as a single strand that folds into a three-dimensional structure that is held in shape by regions of the molecule interacting with each ...
The DnaE polymerase from Deinococcus radiodurans features
... strength conditions or in the presence of manganese. Further assays were performed using a ssDNA and a dsDNA, whose recombination yields a DNA substrate. Surprisingly, αDr was found to be incapable of recombination-dependent DNA polymerase activity, whereas αEc was competent in this action. However, ...
... strength conditions or in the presence of manganese. Further assays were performed using a ssDNA and a dsDNA, whose recombination yields a DNA substrate. Surprisingly, αDr was found to be incapable of recombination-dependent DNA polymerase activity, whereas αEc was competent in this action. However, ...
Lab: Colony PCR amplification of the 16S ribosomal RNA gene I
... Microbial community structure is a critical determinant of the biogeochemical processes occurring within the pelagic marine ecosystem. Due to the difficulties of cultivating many marine microbes, microbial ecologists frequently employ molecular biology-based techniques to reveal the complexity of aq ...
... Microbial community structure is a critical determinant of the biogeochemical processes occurring within the pelagic marine ecosystem. Due to the difficulties of cultivating many marine microbes, microbial ecologists frequently employ molecular biology-based techniques to reveal the complexity of aq ...
Investigating the link between tRNA and mRNA - EMBL-EBI
... of anticodon isoacceptors, each decoding a single codon into its corresponding amino acid. In this thesis I discuss the central importance of the codon– anticodon interface to mrna-to-protein translation, and how its stability is maintained during the life of the cell. This thesis summarises my rese ...
... of anticodon isoacceptors, each decoding a single codon into its corresponding amino acid. In this thesis I discuss the central importance of the codon– anticodon interface to mrna-to-protein translation, and how its stability is maintained during the life of the cell. This thesis summarises my rese ...
Variation in Glutenin Protein Subunits of Wheat
... However, the resolution of protein subunits obtained in these studies was less than ideal because both groups of workers employed a single, continuous-pH gel system. Since discontinuous-pH, two-gel systems, which first concentrate the proteins into a narrow starting zone, generally give better resol ...
... However, the resolution of protein subunits obtained in these studies was less than ideal because both groups of workers employed a single, continuous-pH gel system. Since discontinuous-pH, two-gel systems, which first concentrate the proteins into a narrow starting zone, generally give better resol ...
Ketone Bodies Mimic the Life Span Extending
... The toxicity of ROS/RNS is ameliorated by the NADPH system (Fig. 2), the redox potential of which is made more negative by the metabolism of ketone bodies (19,38,39). The redox potential of the free cytosolic [NADP1]/[NADPH] system is about 20.42 V, about the same redox potential as hydrogen and is ...
... The toxicity of ROS/RNS is ameliorated by the NADPH system (Fig. 2), the redox potential of which is made more negative by the metabolism of ketone bodies (19,38,39). The redox potential of the free cytosolic [NADP1]/[NADPH] system is about 20.42 V, about the same redox potential as hydrogen and is ...
C-terminal Truncation of p21H Preserves Crucial Kinetic and
... for the Cys-A-A-X-OH motif at the endof the chain (with A being an aliphatic residue). This motif has been shown to be responsible for anchoring the protein to the cell membrane (4-8). Due to their homology with other guanine nucleotide binding proteins, it is thought that p21 proteins function as s ...
... for the Cys-A-A-X-OH motif at the endof the chain (with A being an aliphatic residue). This motif has been shown to be responsible for anchoring the protein to the cell membrane (4-8). Due to their homology with other guanine nucleotide binding proteins, it is thought that p21 proteins function as s ...
Protein splicing of PRP8 mini-inteins from species of the genus
... represents a negative control without DNA ...
... represents a negative control without DNA ...
GFP-labelled Rubisco and aspartate aminotransferase are present
... freely through stromules as well. Furthermore, the movement of GFP suggests that macromolecules like DNA and RNA might also traf®c. Stromules may, therefore, function as pathways for movement of metabolites and macromolecules between plastids in different parts of the cell. Hence, stromules may poss ...
... freely through stromules as well. Furthermore, the movement of GFP suggests that macromolecules like DNA and RNA might also traf®c. Stromules may, therefore, function as pathways for movement of metabolites and macromolecules between plastids in different parts of the cell. Hence, stromules may poss ...
Gene Section NOTCH2 (Notch homolog 2 (Drosophila)) Atlas of Genetics and Cytogenetics
... complex with RBP-j kappa and Mastermind proteins and activates genes of the hairy/enhancer of split (hes) family. Notch2 behaves very similar to Notch1 at the biochemical level, and in fact, C-terminal intracellular region of Notch1 can functionally replace that of Notch2 in vivo. However specific f ...
... complex with RBP-j kappa and Mastermind proteins and activates genes of the hairy/enhancer of split (hes) family. Notch2 behaves very similar to Notch1 at the biochemical level, and in fact, C-terminal intracellular region of Notch1 can functionally replace that of Notch2 in vivo. However specific f ...
Cloning and structure of three rainbow trout C3
... the trout C3 isoforms have any related activity ...
... the trout C3 isoforms have any related activity ...
Transfer of genetic material between the
... varying from 11 kbp (Arabidopsis Genome Initiative, 2000) to 20 kbp (Shahmuradov et al., 2003). These fragments were generally ,1 kb in length. This small amount of chloroplast DNA is in contrast to that found in the rice genome where the DNA from the rice chloroplast aligned with a total of between ...
... varying from 11 kbp (Arabidopsis Genome Initiative, 2000) to 20 kbp (Shahmuradov et al., 2003). These fragments were generally ,1 kb in length. This small amount of chloroplast DNA is in contrast to that found in the rice genome where the DNA from the rice chloroplast aligned with a total of between ...
Sequential Expression of Macromolecule
... The ‘late’ expression of the synthesis of antibiotics and other secondary metabolites following intensive growth is a well-known phenomenon. It is equivalent to a differentiation process in which genetic information coding for growth and for antibiotic biosynthesis is sequentially expressed. In broa ...
... The ‘late’ expression of the synthesis of antibiotics and other secondary metabolites following intensive growth is a well-known phenomenon. It is equivalent to a differentiation process in which genetic information coding for growth and for antibiotic biosynthesis is sequentially expressed. In broa ...
Biochemical and Biophysical Research Communications
... germicidal activity to gram-positive bacteria such as Staphylococcus aureus [3,4]. In addition, they have antifungal activity against Leptosphaeria maculans, which causes black root rot of canola [5]. The amino acid chain of fusaricidin is not ribosomally synthesized by encoding, as are other genera ...
... germicidal activity to gram-positive bacteria such as Staphylococcus aureus [3,4]. In addition, they have antifungal activity against Leptosphaeria maculans, which causes black root rot of canola [5]. The amino acid chain of fusaricidin is not ribosomally synthesized by encoding, as are other genera ...
The Invention of Proteomic Code and mRNA
... as the chief proponent of the Big Bang theory in cosmology. He proposed a Diamond Code [4-6] where DNA acted directly as a template for assembling amino acids into proteins. Various combinations of bases along one of the grooves in the double helix could form distinctively shaped cavities into which ...
... as the chief proponent of the Big Bang theory in cosmology. He proposed a Diamond Code [4-6] where DNA acted directly as a template for assembling amino acids into proteins. Various combinations of bases along one of the grooves in the double helix could form distinctively shaped cavities into which ...
mbe.oxfordjournals.org - Oxford Academic
... biogenesis, nucleolar assembly, and regulation of vital cellular processes. However, this nucleolar inventory has not unveiled a specific consensus motif necessary for nucleolar binding. The ribosomal protein family characterized by their basic nature should exhibit distinct binding sequences that e ...
... biogenesis, nucleolar assembly, and regulation of vital cellular processes. However, this nucleolar inventory has not unveiled a specific consensus motif necessary for nucleolar binding. The ribosomal protein family characterized by their basic nature should exhibit distinct binding sequences that e ...
the function and synthesis of ribosomes
... by the long extensions of four r-proteins, but these are too far away to participate directly in catalysis. From this, it was concluded that the peptidyl-transfer reaction is catalysed by RNA1,20. On the basis of the structure of the 50S subunit in complex with analogues that mimic the substrates fo ...
... by the long extensions of four r-proteins, but these are too far away to participate directly in catalysis. From this, it was concluded that the peptidyl-transfer reaction is catalysed by RNA1,20. On the basis of the structure of the 50S subunit in complex with analogues that mimic the substrates fo ...
